Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 682,521 G→C 8.0% A362A (GCC→GCG RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034682,5210G→C 8.0% 44.7 ‑3.0 ‑0.0288A362A (GCC→GCGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (6/17):  ref base (131/133):  total (137/151)
Fisher's exact test strand distribution p-value = 4.81e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.29e-01

Only 100 of 303 total aligned reads displayed.

TCTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCG  >  NC_014034/682421‑682620
                                                                                                    |                                                                                                    
tCTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTgg                                                                                                      <  2:1945615/101‑1 (MQ=255)
 cTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTggg                                                                                                     >  2:314217/1‑101 (MQ=255)
   gcgcGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGcc                                                                                                   >  2:6224446/1‑101 (MQ=255)
     gctgTGGACAGCGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGt                                                                                                 <  2:6924576/98‑1 (MQ=255)
     gcgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGt                                                                                                 <  2:3387638/101‑1 (MQ=255)
      cgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTg                                                                                                <  1:6756648/101‑1 (MQ=255)
        gTGGACAAGGCGTCGATCTGCGCCGAACTCAGAGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCAGCGTGCCCAGAACCACCAGCTGGGCCGTGCt                                                                                              >  1:1287486/1‑101 (MQ=255)
         tGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTc                                                                                             >  1:70176/1‑101 (MQ=255)
         tGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTc                                                                                             <  1:1649839/101‑1 (MQ=255)
         tGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTc                                                                                             >  2:484843/1‑101 (MQ=255)
          ggACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTGCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCtga                                                                                            >  1:3885273/1‑99 (MQ=255)
          ggACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCa                                                                                            <  1:5946774/101‑1 (MQ=255)
          ggACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCa                                                                                            <  1:1050650/101‑1 (MQ=255)
            aCAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgc                                                                                          >  1:5174039/1‑101 (MQ=255)
            aCAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgc                                                                                          >  1:4456422/1‑101 (MQ=255)
             cAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcg                                                                                         >  1:481731/1‑101 (MQ=255)
             cAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcg                                                                                         >  1:2096199/1‑101 (MQ=255)
             cAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcg                                                                                         >  2:211740/1‑101 (MQ=255)
              aaGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcgc                                                                                        <  2:1782713/101‑1 (MQ=255)
              aaGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcgc                                                                                        >  1:1782777/1‑101 (MQ=255)
               aGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGcgcc                                                                                       <  1:2655793/101‑1 (MQ=255)
                   gTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCa                                                                                   <  2:176278/101‑1 (MQ=255)
                    gcGATCTGCGCCGAACCCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCAt                                                                                  <  2:5652698/100‑1 (MQ=255)
                     cGATCTGCGCCGCACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCAAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATc                                                                                 >  1:6405084/1‑101 (MQ=255)
                     cGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATc                                                                                 >  1:5588181/1‑101 (MQ=255)
                      gatcgccgcCCAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCt                                                                                <  2:6944124/95‑1 (MQ=255)
                      gATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCt                                                                                >  2:4218629/1‑101 (MQ=255)
                       aTCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTg                                                                               >  1:920018/1‑101 (MQ=255)
                         cTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTgcg                                                                             >  2:7052802/1‑101 (MQ=255)
                         cTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTgcg                                                                             >  2:5561967/1‑101 (MQ=255)
                          tGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTgcgc                                                                            >  2:3967372/1‑101 (MQ=255)
                          tGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTgcgc                                                                            <  2:1255767/101‑1 (MQ=255)
                            cgcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGccc                                                                          <  1:1683710/101‑1 (MQ=255)
                             gcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGcccc                                                                         <  1:2759366/101‑1 (MQ=255)
                                 aaCTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTc                                                                     >  1:4031977/1‑101 (MQ=255)
                                 aaCTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTc                                                                     >  2:6394900/1‑101 (MQ=255)
                                   cTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAg                                                                   <  1:4921963/101‑1 (MQ=255)
                                   cTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAg                                                                   <  1:5434609/101‑1 (MQ=255)
                                    tCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                  <  1:1468878/101‑1 (MQ=255)
                                     cAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgcg                                                                 >  2:793822/1‑101 (MQ=255)
                                        cgcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccg                                                              <  2:5477250/101‑1 (MQ=255)
                                        cgcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccg                                                              <  1:3789192/101‑1 (MQ=255)
                                         gcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccgc                                                             <  2:2738733/101‑1 (MQ=255)
                                         gcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgccgc                                                             >  2:3846013/1‑101 (MQ=255)
                                          ccTGGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCgccgcc                                                            <  2:2194669/101‑1 (MQ=255)
                                          ccTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCgccgcc                                                            >  1:1586026/1‑101 (MQ=255)
                                            tCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAg                                                          <  2:3251582/101‑1 (MQ=255)
                                             cggcggCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGt                                                         >  1:6081616/1‑101 (MQ=255)
                                              ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                        >  1:6331158/1‑101 (MQ=255)
                                               gcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTc                                                       <  1:1112693/101‑1 (MQ=255)
                                                cggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCg                                                      <  2:2382694/101‑1 (MQ=255)
                                                  gcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGAt                                                    >  2:1592417/1‑101 (MQ=255)
                                                  gcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGAt                                                    <  2:5623203/101‑1 (MQ=255)
                                                     tcctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.t                                                >  2:4586600/1‑101 (MQ=255)
                                                         cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                            >  1:763722/1‑101 (MQ=255)
                                                         cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                            >  1:6962347/1‑101 (MQ=255)
                                                         cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                            >  1:4316127/1‑101 (MQ=255)
                                                         cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTAGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                            >  2:3442580/1‑101 (MQ=255)
                                                         cGGACAGCGCAGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                            >  2:6513449/1‑101 (MQ=255)
                                                          ggACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCa                                           >  2:3552720/1‑101 (MQ=255)
                                                           gACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGGGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAg                                          >  1:3375190/1‑101 (MQ=255)
                                                           gACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAg                                          <  1:281286/101‑1 (MQ=255)
                                                           gACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGCCAGCGCCGCCAGTTCGATCG.TGCTCAg                                          >  1:2299509/1‑101 (MQ=255)
                                                                  ccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCCGcc                                   >  2:6687337/1‑101 (MQ=255)
                                                                      gATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGt                               >  1:2958497/1‑101 (MQ=255)
                                                                       aTCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTc                              >  2:6098344/1‑101 (MQ=255)
                                                                         cTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCt                            >  1:6563995/1‑101 (MQ=255)
                                                                         cTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCt                            <  1:6667593/101‑1 (MQ=255)
                                                                          tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                           <  2:5242545/101‑1 (MQ=255)
                                                                           gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                          >  2:5074117/1‑101 (MQ=255)
                                                                            ccGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCCTGTCCTCgg                         >  2:4101628/1‑101 (MQ=255)
                                                                             cgcgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGt                        <  2:1316721/101‑1 (MQ=255)
                                                                             cgcgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGt                        <  1:5648954/101‑1 (MQ=255)
                                                                              gcgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTc                       <  2:5694793/101‑1 (MQ=255)
                                                                               cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                      >  2:2877453/1‑101 (MQ=255)
                                                                               cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                      <  2:3022142/101‑1 (MQ=255)
                                                                                gTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGa                     <  1:6143026/101‑1 (MQ=255)
                                                                                  gCCCAGACCCCCCAGCTGGGCCGTGCTCAGCGCCCCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaaa                   <  1:3276695/101‑1 (MQ=255)
                                                                                  gCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaaa                   >  2:1986538/1‑101 (MQ=255)
                                                                                  gCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaaa                   <  1:6224765/101‑1 (MQ=255)
                                                                                       gAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcccc              >  2:6304724/1‑101 (MQ=255)
                                                                                       gAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcccc              <  1:966823/101‑1 (MQ=255)
                                                                                         gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt            >  2:5904823/5‑101 (MQ=37)
                                                                                         gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt            <  1:241782/97‑1 (MQ=37)
                                                                                         gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt            <  2:246308/97‑1 (MQ=37)
                                                                                         cccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt            <  2:4166158/97‑1 (MQ=37)
                                                                                         aCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCtt            <  1:271889/101‑1 (MQ=255)
                                                                                          ccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttg           <  2:4808431/98‑2 (MQ=37)
                                                                                          ccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttg           <  1:4647565/98‑2 (MQ=37)
                                                                                          cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc           >  2:5019398/1‑101 (MQ=255)
                                                                                           cgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttga          <  2:3537008/99‑3 (MQ=37)
                                                                                             ccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgc        >  2:4439116/1‑101 (MQ=255)
                                                                                              cAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgcg       >  1:356547/1‑101 (MQ=25)
                                                                                              cAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgcg       >  1:509923/1‑101 (MQ=25)
                                                                                               aGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc      <  1:651113/101‑1 (MQ=255)
                                                                                                 cTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcgg    >  2:3645343/1‑101 (MQ=255)
                                                                                                  tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc   >  2:4930378/1‑101 (MQ=255)
                                                                                                  tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc   >  1:5781970/1‑101 (MQ=255)
                                                                                                   gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg  >  1:3012388/1‑101 (MQ=255)
                                                                                                   gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg  >  2:3412781/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCG  >  NC_014034/682421‑682620

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG