Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 78,028 G→A 100% G150E (GGG→GAG)  aroE → shikimate 5‑dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403478,0280G→A100.0% 1268.6 326G150E (GGG→GAG) aroEshikimate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  new base (131/195):  ref base (0/0):  total (131/195)

Only 100 of 332 total aligned reads displayed.

CCGGGCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGGGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGGATG  >  NC_014034/77931‑78125
                                                                                                 |                                                                                                 
ccGGGCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGAt                                                                                                <  2:6407000/101‑1 (MQ=255)
  gggCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATcc                                                                                              <  1:3844188/101‑1 (MQ=255)
  gggCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATcc                                                                                              <  1:1673516/101‑1 (MQ=255)
  gggCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATcc                                                                                              >  1:4321679/1‑101 (MQ=255)
    gCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGc                                                                                            <  1:4547397/101‑1 (MQ=255)
      tGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAt                                                                                          <  1:1536147/101‑1 (MQ=255)
      tGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAt                                                                                          >  2:6139762/1‑101 (MQ=255)
      gggCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAt                                                                                          <  2:5812301/100‑1 (MQ=255)
        gCAGGCCAGGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAttt                                                                                        <  2:2714596/101‑1 (MQ=255)
        gCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAttt                                                                                        <  1:6781571/101‑1 (MQ=255)
        gCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAttt                                                                                        <  1:6392124/101‑1 (MQ=255)
        gCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAttt                                                                                        >  1:4199763/1‑101 (MQ=255)
         cgggCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTc                                                                                       <  1:3397439/99‑1 (MQ=255)
         cAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTc                                                                                       >  1:556542/1‑101 (MQ=255)
         cAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTc                                                                                       >  2:4565426/1‑101 (MQ=255)
          aGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTcc                                                                                      <  2:3346304/101‑1 (MQ=255)
          aGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTcc                                                                                      <  1:404959/101‑1 (MQ=255)
          aGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTcc                                                                                      >  2:3556131/1‑101 (MQ=255)
             ccAAGGCTGTGCGCGCCGCCGTTATCGGCGCAGACGGTGCTGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCAGGTTGCCCGGAGATCCCCATTTCCAAc                                                                                   <  2:3781546/101‑1 (MQ=255)
             ccAAGGCTGCCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAc                                                                                   <  1:2091083/101‑1 (MQ=255)
              cAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAcc                                                                                  >  1:5890959/1‑101 (MQ=255)
              cAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAcc                                                                                  >  2:1098361/1‑101 (MQ=255)
               aaGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccg                                                                                 >  1:548279/1‑101 (MQ=255)
               aaGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccg                                                                                 >  2:4038282/1‑101 (MQ=255)
               aaGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCAGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccg                                                                                 >  1:5017574/1‑101 (MQ=255)
                gatcTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgc                                                                                <  1:671194/98‑1 (MQ=255)
                   cTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacc                                                                             <  2:1616091/101‑1 (MQ=255)
                    tGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgccccc                                                                            >  2:1485890/1‑101 (MQ=255)
                    tGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcaccc                                                                            <  2:6860693/101‑1 (MQ=255)
                    tGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcaccc                                                                            >  1:2722954/1‑101 (MQ=255)
                    tGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcaccc                                                                            >  2:1772613/1‑101 (MQ=255)
                    gggCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcaccc                                                                            <  1:1972190/100‑1 (MQ=255)
                     ggCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccg                                                                           <  2:5384716/101‑1 (MQ=255)
                     ggCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccg                                                                           <  2:5976322/101‑1 (MQ=255)
                      gCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccgc                                                                          >  1:4807180/1‑101 (MQ=255)
                       ccccGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccgca                                                                         <  1:1518518/101‑1 (MQ=255)
                        cccGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccgcac                                                                        >  1:6987948/1‑101 (MQ=255)
                        cccGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccgcac                                                                        <  2:202356/101‑1 (MQ=255)
                         ccgccgccgTCATCGGTGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACcgcacccgcacc                                                                       <  2:2870303/101‑1 (MQ=255)
                            ccgccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCgg                                                                    <  1:5813092/101‑1 (MQ=255)
                             cgccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCggg                                                                   <  1:204025/101‑1 (MQ=255)
                              gccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGc                                                                  <  1:2393116/101‑1 (MQ=255)
                              gccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGc                                                                  <  2:5314368/101‑1 (MQ=255)
                               ccgTCATCCGCGCCGGCGGGGCCCCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGcc                                                                 <  2:5188698/101‑1 (MQ=255)
                                cgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCg                                                                <  1:6572373/101‑1 (MQ=255)
                                    aTCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGt                                                            >  2:128992/1‑101 (MQ=255)
                                         cgcAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCttt                                                       <  1:1095425/101‑1 (MQ=255)
                                         cgcAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCttt                                                       <  2:4262691/101‑1 (MQ=255)
                                         cgcAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGGGCttt                                                       >  2:6659334/1‑101 (MQ=255)
                                          gcAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTg                                                      <  1:2826244/101‑1 (MQ=255)
                                            cggCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTgcg                                                    <  1:880941/100‑1 (MQ=255)
                                            aGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTgcg                                                    >  1:5135330/1‑101 (MQ=255)
                                                ggTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCTCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGa                                                <  2:4862326/101‑1 (MQ=255)
                                                   gccgccCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAAtt                                             >  1:1903575/1‑101 (MQ=255)
                                                   gccgccCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAAtt                                             <  2:1876545/101‑1 (MQ=255)
                                                   gccgccCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAAtt                                             <  2:4651945/101‑1 (MQ=255)
                                                    ccgcccgcGGCATCGTCGTCTCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCCCCCGCGCCCGGGCCGAGGTGCTTTGCGCCGAATTc                                            <  1:4388080/101‑1 (MQ=255)
                                                    ccgcccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTc                                            <  2:2071542/101‑1 (MQ=255)
                                                    ccgcccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTc                                            <  2:2419552/101‑1 (MQ=255)
                                                    ccgcccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTc                                            <  2:2546362/101‑1 (MQ=255)
                                                    ccgcccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTc                                            <  2:3002615/101‑1 (MQ=255)
                                                     cgcccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCg                                           >  2:6611695/1‑101 (MQ=255)
                                                        ccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGcc                                        >  1:6896046/1‑101 (MQ=255)
                                                         cgcgCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCCCCCGGGCCGAGGTGCTTTGCGCCGAATTCGGccc                                       <  1:4571872/101‑1 (MQ=255)
                                                           cgcCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGccccc                                     <  1:6077938/101‑1 (MQ=255)
                                                            gcCATCGTCGTCGCCCGGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCcg                                    <  1:5700164/101‑1 (MQ=255)
                                                              cATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCcgcg                                  >  2:739988/1‑101 (MQ=255)
                                                               aTCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCcgcgc                                 >  2:6467212/1‑100 (MQ=255)
                                                               aTCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgt                                 >  1:1241168/1‑101 (MQ=255)
                                                               aTCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgt                                 <  1:1173449/101‑1 (MQ=255)
                                                                tcgtcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtc                                >  1:7005874/1‑101 (MQ=255)
                                                                 cgtcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtcg                               <  2:548267/101‑1 (MQ=255)
                                                                 cgtcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtcg                               >  1:5410214/1‑101 (MQ=255)
                                                                 cgtcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtcg                               >  2:675593/1‑101 (MQ=255)
                                                                  gtcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtcgt                              >  2:702961/1‑101 (MQ=255)
                                                                    cgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTgg                            >  1:3960960/1‑101 (MQ=255)
                                                                     gtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGt                           <  1:1592197/101‑1 (MQ=255)
                                                                     gtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGt                           <  2:5732586/101‑1 (MQ=255)
                                                                      tcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGtt                          <  1:2539828/101‑1 (MQ=255)
                                                                      tcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGtt                          <  2:3432467/101‑1 (MQ=255)
                                                                      tcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGtt                          <  1:3346958/101‑1 (MQ=255)
                                                                       cgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTc                         <  2:6964597/101‑1 (MQ=255)
                                                                        gCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGATGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCa                        <  1:1837752/101‑1 (MQ=255)
                                                                           cTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGa                     >  2:3643199/1‑101 (MQ=255)
                                                                           cTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGa                     <  1:1340765/101‑1 (MQ=255)
                                                                             gCTCGAGGCAGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGAtt                   >  2:219787/1‑101 (MQ=255)
                                                                               tCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTgg                 <  2:1178569/101‑1 (MQ=255)
                                                                               tCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTgg                 >  1:2463865/1‑101 (MQ=255)
                                                                               tCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGGGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTgg                 >  2:3863178/1‑101 (MQ=255)
                                                                                cGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTggg                >  1:3129519/1‑101 (MQ=255)
                                                                                cGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTggg                >  1:369446/1‑101 (MQ=255)
                                                                                  aGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGgag              <  2:2671991/101‑1 (MQ=255)
                                                                                    gCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGaa            >  2:4339249/1‑101 (MQ=255)
                                                                                      cGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAgg          <  1:3643348/101‑1 (MQ=255)
                                                                                       ggTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGt         >  2:5010581/1‑101 (MQ=255)
                                                                                       ggTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGt         >  1:7093737/1‑101 (MQ=255)
                                                                                        gTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGTCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTc        <  2:5890657/101‑1 (MQ=255)
                                                                                             ccGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgat   >  2:4787971/1‑101 (MQ=255)
                                                                                             ccGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgat   >  2:2366267/1‑101 (MQ=255)
                                                                                              cGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgatg  <  1:2936932/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
CCGGGCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGGGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGGATG  >  NC_014034/77931‑78125

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG