Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 833,231 C→A 100% G35V (GGC→GTC)  mcpA1 ← methyl‑accepting chemotaxis protein McpA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034833,2310C→A100.0% 1591.2 415G35V (GGC→GTC) mcpA1methyl‑accepting chemotaxis protein McpA
Reads supporting (aligned to +/- strand):  new base (207/206):  ref base (1/1):  total (208/207)

Only 100 of 420 total aligned reads displayed.

TGCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGCCCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGTT  >  NC_014034/833133‑833328
                                                                                                  |                                                                                                 
 gCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCCTGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCg                                                                                                <  2:4586997/101‑1 (MQ=255)
      gttGCACCTGTGCTTCCAGATGGATCAGCATGCCGGTGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaaa                                                                                           <  2:6523835/100‑1 (MQ=255)
      cTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaaa                                                                                           <  2:6081289/101‑1 (MQ=255)
      attGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaaa                                                                                           <  1:838435/100‑1 (MQ=255)
       ttGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAAc                                                                                          <  1:5323969/101‑1 (MQ=255)
       ttGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAAc                                                                                          <  2:4720767/101‑1 (MQ=255)
        tGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACg                                                                                         <  1:442579/101‑1 (MQ=255)
         gCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGc                                                                                        <  1:136458/101‑1 (MQ=255)
         gCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGc                                                                                        <  1:3467327/101‑1 (MQ=255)
         gCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGGGTCGGGGACGTCGCTGAGGGGTTGTTCGCGCATCCCATAGGCGTTCGAGCCGCCAAAACGc                                                                                        >  1:3897539/1‑101 (MQ=255)
          cACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCa                                                                                       >  1:1083347/1‑101 (MQ=255)
             cTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCa                                                                                    <  2:6967821/101‑1 (MQ=255)
               gtgCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGc                                                                                  <  2:3872547/101‑1 (MQ=255)
                tgCTTCCAGGTCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCg                                                                                 <  2:134710/101‑1 (MQ=255)
                  tctccaaaaggatcAGGAATCCGACGCCGGTTTCCGTGACGTCCCTGACGTGTTTTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGtt                                                                               <  2:2271234/91‑1 (MQ=255)
                   ttgcggctcgattGGCATGCCGGTGGGGGTGTGGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGttt                                                                              <  2:2317731/94‑1 (MQ=255)
                    tCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTc                                                                             <  1:4814935/101‑1 (MQ=255)
                      cAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCgg                                                                           <  2:2730943/101‑1 (MQ=255)
                      cAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCgg                                                                           <  2:5502729/101‑1 (MQ=255)
                       aGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGa                                                                          <  1:1505722/101‑1 (MQ=255)
                       aGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGa                                                                          <  1:2430570/101‑1 (MQ=255)
                        gatcgatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGAc                                                                         >  1:4064279/1‑101 (MQ=255)
                          tcgatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACaa                                                                       <  1:5501305/101‑1 (MQ=255)
                          tcgatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACaa                                                                       >  2:1709076/1‑101 (MQ=255)
                           cgatcAGCATGCCGATGGCGGTGTCGGTGACGTGGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAg                                                                      >  1:5005598/1‑101 (MQ=255)
                            gatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTTAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAga                                                                     <  1:6338691/101‑1 (MQ=255)
                            gatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAga                                                                     >  2:5126951/1‑101 (MQ=255)
                            gatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAga                                                                     <  2:3780698/101‑1 (MQ=255)
                             atcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAgat                                                                    >  2:3529788/1‑101 (MQ=255)
                                 gcgtGCCGATGGGGGTGTCGGTGACGGCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGatgatg                                                                <  1:6941006/98‑1 (MQ=255)
                                  cATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGAtgatga                                                               >  1:6966295/1‑101 (MQ=255)
                                  cATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGAtgatga                                                               <  1:5570179/101‑1 (MQ=255)
                                   aTGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAg                                                              >  1:1676972/1‑101 (MQ=255)
                                        gATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGaaa                                                         >  2:5018839/1‑101 (MQ=255)
                                        gATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGaaa                                                         <  2:5592141/101‑1 (MQ=255)
                                        gATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGaaa                                                         <  1:3841560/101‑1 (MQ=255)
                                        gATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGaaa                                                         <  1:4272491/101‑1 (MQ=255)
                                        gATGGCGGTGTCGGTGACGTCGCTGAGGTGTTATTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGaaa                                                         <  1:5956590/101‑1 (MQ=255)
                                         aTGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGCCCGCAAAACGGACCAGCGCTTCCGACAAGATGATGAGCGAGAc                                                        >  2:7076556/1‑101 (MQ=255)
                                         aTGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAc                                                        >  2:3590688/1‑101 (MQ=255)
                                         aTGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAc                                                        >  1:2834501/1‑101 (MQ=255)
                                         aTGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAc                                                        <  2:4857925/101‑1 (MQ=255)
                                         aTGGCGGTGTCGGTGACGTCGCTGAGGCGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAc                                                        >  1:6144528/1‑101 (MQ=255)
                                          tGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcg                                                       >  1:1932196/1‑101 (MQ=255)
                                           ggCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcgg                                                      >  2:3173589/1‑101 (MQ=255)
                                              ggTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcggcgg                                                   >  1:5801810/1‑101 (MQ=255)
                                                tgtCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCa                                                 >  2:1844330/1‑101 (MQ=255)
                                                 gtCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCaa                                                >  2:903987/1‑101 (MQ=255)
                                                 gtCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCaa                                                >  2:6833744/1‑101 (MQ=255)
                                                   cGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAgg                                              >  1:7037509/1‑101 (MQ=255)
                                                     gTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGc                                            >  2:6367865/1‑101 (MQ=255)
                                                      tGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGcc                                           >  1:6025467/1‑101 (MQ=255)
                                                      tGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGcc                                           >  2:1203895/1‑101 (MQ=255)
                                                      tGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGcc                                           >  2:1472587/1‑101 (MQ=255)
                                                      tGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGcc                                           <  1:3529924/101‑1 (MQ=255)
                                                          gTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGa                                       >  2:756000/1‑101 (MQ=255)
                                                             gCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACTATCg                                    >  2:4519397/1‑101 (MQ=255)
                                                              cTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGc                                   <  1:2977348/101‑1 (MQ=255)
                                                              cTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGc                                   >  1:2249383/1‑101 (MQ=255)
                                                              cTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGc                                   >  2:2007401/1‑101 (MQ=255)
                                                               tGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCa                                  >  2:4030424/1‑101 (MQ=255)
                                                               tGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCa                                  <  2:2948880/101‑1 (MQ=255)
                                                                gAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCAt                                 <  1:2087796/101‑1 (MQ=255)
                                                                gAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCAt                                 >  1:4158995/1‑101 (MQ=255)
                                                                  ggTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAg                               >  1:4782563/1‑101 (MQ=255)
                                                                  ggTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAg                               <  1:1226973/101‑1 (MQ=255)
                                                                  ggTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAg                               >  2:415970/1‑101 (MQ=255)
                                                                  ggTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAg                               >  1:5442038/1‑101 (MQ=255)
                                                                   gtgtTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCGACGATCGCATAGa                              >  2:5690751/1‑101 (MQ=255)
                                                                   gtgtTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCCAGCTCGCATAGa                              >  1:6231222/1‑101 (MQ=255)
                                                                   gtgtTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGa                              <  2:2797051/101‑1 (MQ=255)
                                                                   gtgtTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGa                              >  1:6805470/1‑101 (MQ=255)
                                                                    tgttgtTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCCCGATCGCATAGAt                             >  2:5871658/1‑101 (MQ=255)
                                                                     gttgttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATg                            <  1:2047241/101‑1 (MQ=255)
                                                                       tgttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcg                          <  1:673985/101‑1 (MQ=255)
                                                                       tgttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcg                          <  2:60083/101‑1 (MQ=255)
                                                                         ttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcggg                        <  1:4947964/101‑1 (MQ=255)
                                                                              gcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaa                   <  2:253926/101‑1 (MQ=255)
                                                                               cATCTCATAGGCGTTGGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaaa                  >  1:5628101/1‑101 (MQ=255)
                                                                                 tctcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATa                <  1:5174370/101‑1 (MQ=255)
                                                                                 tctcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGACACGATCGCATAGATGCGGGCGGAAATa                <  1:363544/101‑1 (MQ=255)
                                                                                   tcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAACAcc              >  2:4370315/1‑101 (MQ=255)
                                                                                    cATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCg             <  1:3722385/101‑1 (MQ=255)
                                                                                    cATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCg             >  2:5196961/1‑101 (MQ=255)
                                                                                    cATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCg             >  1:3067098/1‑101 (MQ=255)
                                                                                     aTAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCga            >  1:3181016/1‑101 (MQ=255)
                                                                                     aTAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCga            <  1:2907156/101‑1 (MQ=255)
                                                                                      tAGGCGTTCGAGACCGCAAAACGCACCAGCGGTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaa           <  1:4642377/101‑1 (MQ=255)
                                                                                       aGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaag          >  1:6456202/1‑101 (MQ=255)
                                                                                       aGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaag          <  1:7096860/101‑1 (MQ=255)
                                                                                       aGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaag          <  2:3678326/101‑1 (MQ=255)
                                                                                         gCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaagaa        <  1:5148472/101‑1 (MQ=255)
                                                                                          cGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAt       <  2:3346112/101‑1 (MQ=255)
                                                                                            ttCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAttt     >  2:6238580/1‑101 (MQ=255)
                                                                                             tCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAACCGGCGCCAAGGCCCACGATCGCATAGAAGCGGGCGGCCATTCCGAAGAATTTg    >  1:2677838/1‑101 (MQ=255)
                                                                                             tCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTg    <  1:2512541/101‑1 (MQ=255)
                                                                                              cGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGt   >  1:6585573/1‑101 (MQ=255)
                                                                                              cGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGt   <  2:5405638/101‑1 (MQ=255)
                                                                                               gAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGtt  >  2:1026180/1‑101 (MQ=255)
                                                                                               gAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGtt  >  2:2108522/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGCCCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGTT  >  NC_014034/833133‑833328

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG