Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 840,920 C→G 100% G188G (GGC→GGG hypF → hydrogenase maturation protein HypF

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034840,9200C→G100.0% 855.4 230G188G (GGC→GGGhypFhydrogenase maturation protein HypF
Reads supporting (aligned to +/- strand):  new base (108/122):  ref base (0/0):  total (108/122)

Only 100 of 250 total aligned reads displayed.

TGCCGGGCGGAATATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGCGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCATCTGGCCT  >  NC_014034/840822‑841017
                                                                                                  |                                                                                                 
tGCCGGGCGGAATATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGgggc                                                                                                 >  1:134364/1‑101 (MQ=255)
   cGGGCGGAATATGAAGCCCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGa                                                                                              <  1:5575819/101‑1 (MQ=255)
    gggCGGAATATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAg                                                                                             <  2:5916863/101‑1 (MQ=255)
     ggCGGAATATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAgg                                                                                            >  2:6733359/1‑100 (MQ=255)
       cGGAATATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTg                                                                                          >  2:835060/1‑101 (MQ=255)
         gAATATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGcc                                                                                        >  1:5793711/1‑101 (MQ=255)
         gAATATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGcc                                                                                        >  2:5968822/1‑101 (MQ=255)
          aaTATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCg                                                                                       >  2:5103137/1‑101 (MQ=255)
          aaTATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCg                                                                                       >  2:6533571/1‑101 (MQ=255)
           atatGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCgg                                                                                      >  1:219526/1‑101 (MQ=255)
            tatGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCggg                                                                                     >  2:2821148/1‑101 (MQ=255)
            tatGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCggg                                                                                     >  1:2870064/1‑101 (MQ=255)
            tatGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCggg                                                                                     >  2:1843201/1‑101 (MQ=255)
            tatGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCggg                                                                                     >  2:1663389/1‑101 (MQ=255)
             atGAAGCCCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgc                                                                                    <  2:6427964/101‑1 (MQ=255)
             atGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgc                                                                                    >  1:1846766/1‑101 (MQ=255)
             atGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgc                                                                                    >  2:745220/1‑101 (MQ=255)
              tGAAGCCCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgcg                                                                                   <  2:1340101/101‑1 (MQ=255)
               gAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgcga                                                                                  >  1:2591862/1‑101 (MQ=255)
               gAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgcga                                                                                  >  1:1078056/1‑101 (MQ=255)
                aaGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgcgat                                                                                 >  2:268566/1‑101 (MQ=255)
                  gcccccGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgcgatgc                                                                               <  2:1488535/99‑1 (MQ=255)
                   gccccGCCGACCGCCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgcgatgcg                                                                              <  2:2964054/100‑1 (MQ=255)
                   cccccGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGgcgatgcg                                                                              <  1:2064737/100‑1 (MQ=255)
                      ccgccgACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATc                                                                           >  2:1929924/1‑101 (MQ=255)
                      ccgccgACCGGCCCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATc                                                                           <  2:2481583/101‑1 (MQ=255)
                       cgccgACCGGCGCTTTCCCGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCg                                                                          <  2:4482100/101‑1 (MQ=255)
                        gccgACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGCAGGCGGGCGGGGCGGCGCTGCCGGGCGATGCGAGCgg                                                                         >  2:3137411/1‑101 (MQ=255)
                         ccgACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGt                                                                        >  1:7047600/1‑101 (MQ=255)
                           gACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCt                                                                      >  2:4382430/1‑101 (MQ=255)
                            cccGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTg                                                                     <  1:3743515/100‑1 (MQ=255)
                            aCCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTg                                                                     <  1:2821250/101‑1 (MQ=255)
                            aCCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTg                                                                     <  2:112620/101‑1 (MQ=255)
                            aCCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTg                                                                     <  2:928943/101‑1 (MQ=255)
                            aCCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTg                                                                     >  2:6108811/1‑101 (MQ=255)
                              cGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgc                                                                   >  1:978481/1‑101 (MQ=255)
                              cGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgc                                                                   >  1:5553029/1‑101 (MQ=255)
                               ggCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgcc                                                                  <  1:6999653/101‑1 (MQ=255)
                               ggCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgcc                                                                  <  2:6264317/101‑1 (MQ=255)
                                 cgcTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgccgc                                                                <  2:4742978/101‑1 (MQ=255)
                                  gcTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgccgcc                                                               >  2:4733376/1‑101 (MQ=255)
                                    tttCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgccgccgc                                                             >  2:6885699/1‑101 (MQ=255)
                                    tttCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgccgccgc                                                             >  2:6823503/1‑101 (MQ=255)
                                    tttCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgccgccgc                                                             >  2:3655383/1‑101 (MQ=255)
                                    tttCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgccgccgc                                                             >  1:5434101/1‑101 (MQ=255)
                                     ttCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgccgccgcc                                                            >  1:1639704/1‑101 (MQ=255)
                                     ttCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGgccgccgcc                                                            >  1:721667/1‑101 (MQ=255)
                                      tCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGccc                                                           <  2:3448348/101‑1 (MQ=255)
                                      tCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGccc                                                           <  1:6412978/101‑1 (MQ=255)
                                       cACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCg                                                          <  2:1511687/101‑1 (MQ=255)
                                       cACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCg                                                          >  1:6612643/1‑101 (MQ=255)
                                        aCGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCgg                                                         >  1:1087449/1‑101 (MQ=255)
                                         cGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGc                                                        <  1:6733701/101‑1 (MQ=255)
                                           cccagcccaTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTg                                                      <  2:4167575/101‑1 (MQ=255)
                                              agcccaTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGCAg                                                   >  2:5676754/1‑101 (MQ=255)
                                                cccaTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGc                                                 <  1:1774170/101‑1 (MQ=255)
                                                cccaTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGc                                                 <  2:5040677/101‑1 (MQ=255)
                                                cccaTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGc                                                 >  2:2436470/1‑101 (MQ=255)
                                                 ccaTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGcc                                                >  1:4319170/1‑101 (MQ=255)
                                                  caTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCg                                               >  2:5186138/1‑101 (MQ=255)
                                                  caTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCg                                               >  2:5794169/1‑101 (MQ=255)
                                                   aTCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCgg                                              >  2:2695602/1‑101 (MQ=255)
                                                     cGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCgggg                                            >  1:7128154/1‑101 (MQ=255)
                                                      gCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGa                                           <  1:5902955/101‑1 (MQ=255)
                                                      gCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGa                                           <  1:5969126/101‑1 (MQ=255)
                                                       ccTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGaa                                          <  2:2012250/101‑1 (MQ=255)
                                                         tGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAAt                                        >  2:4295160/1‑101 (MQ=255)
                                                          gCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATGGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGGAATc                                       >  1:2368405/1‑101 (MQ=255)
                                                             ccGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCtt                                    >  2:305015/1‑101 (MQ=255)
                                                             ccGATTGCGGGCCGCGGCTCCGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCtt                                    <  1:6844651/101‑1 (MQ=255)
                                                              cGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTg                                   >  2:2043965/1‑101 (MQ=255)
                                                                aTTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCg                                 >  2:7058880/1‑101 (MQ=255)
                                                                 ttGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCgg                                <  1:6679980/101‑1 (MQ=255)
                                                                     gggCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGaa                            <  1:4518240/101‑1 (MQ=255)
                                                                     gggCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGaa                            <  2:4054932/101‑1 (MQ=255)
                                                                     gggCCGCGGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGaa                            >  1:922847/1‑101 (MQ=255)
                                                                       gcggcgGCTCAGGCTAGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAg                          <  2:5312241/98‑1 (MQ=255)
                                                                         cgcgGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAggg                        >  1:4183415/1‑101 (MQ=255)
                                                                          gcgGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGc                       >  1:4791132/1‑101 (MQ=255)
                                                                           cgGCTCTGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCt                      <  1:352106/101‑1 (MQ=255)
                                                                              ctctGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTgg                   >  1:77248/1‑101 (MQ=255)
                                                                              ctctGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTgg                   <  1:5303178/101‑1 (MQ=255)
                                                                              ctctGGCTGGAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGGGAAAGGGCTTgg                   <  1:5112775/101‑1 (MQ=255)
                                                                                 tggctggAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTggcgg                >  2:6626327/1‑101 (MQ=255)
                                                                                   gctggAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCt              <  1:3970726/101‑1 (MQ=255)
                                                                                   gctggAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCt              >  1:4905879/1‑101 (MQ=255)
                                                                                     tggAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCttt            <  1:1429011/101‑1 (MQ=255)
                                                                                     tggAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCttt            >  2:3830149/1‑101 (MQ=255)
                                                                                      ggAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTc           <  2:3599593/101‑1 (MQ=255)
                                                                                      ggAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTc           <  1:1662151/101‑1 (MQ=255)
                                                                                       gcggcgggagGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCa          <  2:4544703/99‑1 (MQ=255)
                                                                                       gAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCa          <  2:4172712/101‑1 (MQ=255)
                                                                                       gAGGCGGGCGGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCa          <  2:334274/101‑1 (MQ=255)
                                                                                         ggcgggcgGGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCATc        <  2:219523/101‑1 (MQ=255)
                                                                                           cgggcggGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCATCTg      >  1:601397/1‑101 (MQ=255)
                                                                                            gggcggGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCATCTgg     >  1:2727103/1‑101 (MQ=255)
                                                                                            gggcggGGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCATCTgg     <  2:1274571/101‑1 (MQ=255)
                                                                                             ggcgggGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCATCTGGc    >  1:4720507/1‑101 (MQ=255)
                                                                                             ggcgggGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCGTGGCGGCCTTCATCTGGc    >  1:2306142/1‑101 (MQ=255)
                                                                                               cggggCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCATCTGGCCt  >  1:6511704/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGCCGGGCGGAATATGAAGACCCCGCCGACCGGCGCTTTCACGCCCAGCCCATCGCCTGCCCCGATTGCGGGCCGCGGCTCTGGCTGGAGGCGGGCGGCGCGGAGCTGCCGGGCGATGCGATCGGTCTGGCCGCCGCCCGGCTGAAGGCCGGGGAAATCCTTGCGGTGAAAGGGCTTGGCGGCTTTCATCTGGCCT  >  NC_014034/840822‑841017

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG