Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 915,073 G→C 4.9% intergenic (+54/+35) recN → / ← RCAP_rcc00830 DNA repair protein RecN/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034915,0730G→C 4.9% 19.9 0.0 ‑0.0265intergenic (+54/+35)recN/RCAP_rcc00830DNA repair protein RecN/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/9):  ref base (77/175):  total (81/184)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-01

Only 100 of 284 total aligned reads displayed.

TGACCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGCGT  >  NC_014034/914973‑915173
                                                                                                    |                                                                                                    
tGACCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTgg                                                                                                      <  2:122703/101‑1 (MQ=255)
 gACCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGc                                                                                                     <  2:4648147/101‑1 (MQ=255)
 gACCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTATGGc                                                                                                     <  2:1901426/101‑1 (MQ=255)
   ccGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCaa                                                                                                   <  1:2561615/101‑1 (MQ=255)
    ccggaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAg                                                                                                  >  1:6554829‑M1/48‑101 (MQ=255)
    ccggaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAg                                                                                                  >  2:4501753‑M1/48‑101 (MQ=255)
    cGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAg                                                                                                  <  2:6924933/101‑1 (MQ=255)
    cGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAg                                                                                                  <  1:838536/101‑1 (MQ=255)
    cGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAg                                                                                                  <  2:6141982/101‑1 (MQ=255)
     cggaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCCGGATATTTCCGCCAagg                                                                                                 <  1:2991033‑M1/55‑2 (MQ=255)
      ggaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCaaggg                                                                                                <  1:3514120‑M1/56‑3 (MQ=255)
      ggaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCaaggg                                                                                                <  1:3079740‑M1/56‑3 (MQ=255)
      gcgccgcCCGGGCCGCGGCGCAATCGCTTCTGTACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGAt                                                                                                <  2:5255137/100‑1 (MQ=255)
       gaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCcaagggg                                                                                               <  2:6722285‑M1/57‑4 (MQ=255)
       gaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCcaagggg                                                                                               <  2:5078579‑M1/57‑4 (MQ=255)
       gaaccgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCcaagggg                                                                                               >  2:1800881‑M1/45‑98 (MQ=255)
        gccgccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaa                                                                                              <  1:2801256/101‑1 (MQ=255)
        gccgccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaa                                                                                              <  2:2508381/101‑1 (MQ=255)
        gccgccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATaa                                                                                              <  1:4237600/101‑1 (MQ=255)
         accgggggccttcgggcccccgctttcggtccgcccgaagagGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCgccaaggggaa                                                                                             >  1:1494929‑M1/43‑96 (MQ=255)
           gccCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAg                                                                                           <  1:562993/101‑1 (MQ=255)
             ccGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAggg                                                                                         <  1:5406591/101‑1 (MQ=255)
              cGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGc                                                                                        <  2:140267/101‑1 (MQ=255)
              cGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGc                                                                                        <  1:5935243/101‑1 (MQ=255)
              cGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGc                                                                                        <  2:6076651/101‑1 (MQ=255)
                    gcggcgCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGc                                                                                  <  2:1496354/101‑1 (MQ=255)
                     cggcgCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCt                                                                                 <  2:6682032/101‑1 (MQ=255)
                     cggcgCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCt                                                                                 <  2:899824/101‑1 (MQ=255)
                      ggcgCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTg                                                                                <  1:4128298/101‑1 (MQ=255)
                       gcgcAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAACAGGGCAGGGGCTGt                                                                               <  1:5572282/101‑1 (MQ=255)
                        cgcAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTc                                                                              <  1:2144279/101‑1 (MQ=255)
                         gcAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTcc                                                                             <  2:3893744/101‑1 (MQ=255)
                           aaTCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTcccc                                                                           <  1:1711928/101‑1 (MQ=255)
                             tCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTcccccc                                                                         <  2:4011412/101‑1 (MQ=255)
                               gCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCt                                                                       <  1:2661062/101‑1 (MQ=255)
                               gCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCt                                                                       <  1:4664060/101‑1 (MQ=255)
                               gCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCt                                                                       <  2:2775272/101‑1 (MQ=255)
                                  tCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGcc                                                                    <  2:6391282/101‑1 (MQ=255)
                                    tGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCtt                                                                  <  1:4900731/101‑1 (MQ=255)
                                     gCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTa                                                                 <  1:6333611/101‑1 (MQ=255)
                                     gCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTa                                                                 <  2:6019012/101‑1 (MQ=255)
                                         ggCTGAGGCAGGGGGGCGCTGCCCGCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGAt                                                             <  2:7034138/101‑1 (MQ=255)
                                            tGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTcc                                                          <  1:5899521/101‑1 (MQ=255)
                                               ggCAGGGGGGGGGGGCCCCCCCTCCCCGCGGGGCTCCCCCCCGGGTATTTCTGGCAAGATAAAAAGGCAGGGGGCGGCGCCCCCCGCCTTCCGCTTccacc                                                       >  2:4236609/1‑101 (MQ=255)
                                               ggCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTccacc                                                       <  2:2104994/101‑1 (MQ=255)
                                                gCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTccacca                                                      <  1:1358341/101‑1 (MQ=255)
                                                 cAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCcaccac                                                     <  2:6800869/101‑1 (MQ=255)
                                                 cAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCcaccac                                                     >  2:6582657/1‑101 (MQ=255)
                                                 cAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCcaccac                                                     >  1:5311212/1‑101 (MQ=255)
                                                  aGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACg                                                    <  2:2349878/101‑1 (MQ=255)
                                                    gggggCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGaa                                                  <  2:6531098/101‑1 (MQ=255)
                                                      gggCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAAtt                                                <  2:1685297/101‑1 (MQ=255)
                                                      gggCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGACATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATGCCACCACGAAtt                                                <  1:5094000/101‑1 (MQ=255)
                                                       ggCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTg                                               <  1:2870246/101‑1 (MQ=255)
                                                         cgcTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTgcg                                             >  2:3596971/1‑101 (MQ=255)
                                                          gcTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTgcgc                                            >  1:4589396/1‑101 (MQ=255)
                                                            tGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCgg                                          >  2:4791204/1‑101 (MQ=255)
                                                            tGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCgg                                          >  2:6346826/1‑101 (MQ=255)
                                                             gtcccccGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGTGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGt                                         <  1:3890329/99‑1 (MQ=255)
                                                             gCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGt                                         >  1:906206/1‑101 (MQ=255)
                                                             gCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGt                                         >  2:4749286/1‑101 (MQ=255)
                                                               cccccGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGa                                       <  2:4692166/101‑1 (MQ=255)
                                                                ccccGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGAc                                      <  2:2225262/101‑1 (MQ=255)
                                                                 cccGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACg                                     >  1:5005020/1‑101 (MQ=255)
                                                                 cccGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACg                                     <  1:4375941/101‑1 (MQ=255)
                                                                   cGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGAt                                   >  2:5281602/1‑101 (MQ=255)
                                                                   cGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGAt                                   <  1:7038043/101‑1 (MQ=255)
                                                                      cccTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTg                                <  1:6963155/101‑1 (MQ=255)
                                                                         tGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCa                             <  2:2947506/101‑1 (MQ=255)
                                                                         tGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCa                             >  2:5527800/1‑101 (MQ=255)
                                                                          gCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCaa                            >  2:336089/1‑101 (MQ=255)
                                                                          gCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCaa                            >  1:3956577/1‑101 (MQ=255)
                                                                            gggACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGCCGATCTGCCAATc                          >  1:1253377/1‑101 (MQ=255)
                                                                            gggACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATc                          >  1:1065314/1‑101 (MQ=255)
                                                                             tctcTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATcc                         <  1:1660432/98‑1 (MQ=255)
                                                                             ggACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATcc                         >  2:1278308/1‑101 (MQ=255)
                                                                              gACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCt                        >  2:5731525/1‑101 (MQ=255)
                                                                              gACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCt                        <  1:6645446/101‑1 (MQ=255)
                                                                                 tCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCgg                     >  2:3736332/1‑101 (MQ=255)
                                                                                 cccgcccGGGAGATGTCTGGGAAGAGAAAAGGGCAGGGGCTGTCCCCCCGTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCgg                     <  1:1912631/97‑1 (MQ=255)
                                                                                    ccccGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGGCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGc                  >  2:5964294/1‑101 (MQ=255)
                                                                                     cccGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGcc                 <  2:6216720/101‑1 (MQ=255)
                                                                                      ccGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCg                >  1:4872413/1‑101 (MQ=255)
                                                                                       cGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGa               <  1:3401408/101‑1 (MQ=255)
                                                                                        gggATATTTCTGGCAAGATAAAAGGGCAAGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAg              >  1:3766721/1‑101 (MQ=255)
                                                                                         ggATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAgg             >  1:243020/1‑101 (MQ=255)
                                                                                         ggATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAgg             >  2:1588788/1‑101 (MQ=255)
                                                                                             atTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGtt         <  2:732739/101‑1 (MQ=255)
                                                                                             atTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGtt         >  1:1013988/1‑101 (MQ=255)
                                                                                             atTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGtt         >  2:2250050/1‑101 (MQ=255)
                                                                                             atTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGtt         >  1:6746299/1‑101 (MQ=255)
                                                                                              tttCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTc        >  2:4391856/1‑101 (MQ=255)
                                                                                              tttCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTc        <  1:3207791/101‑1 (MQ=255)
                                                                                              tttCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTc        <  1:6678915/101‑1 (MQ=255)
                                                                                               ttCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCg       >  1:2238939/1‑101 (MQ=255)
                                                                                                tCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGa      <  2:325232/101‑1 (MQ=255)
                                                                                                tCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGa      <  1:3857680/101‑1 (MQ=255)
                                                                                                  tGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGc    >  1:4484314/1‑101 (MQ=255)
                                                                                                   ggCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGCg   >  1:1718779/1‑101 (MQ=255)
                                                                                                    gCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGCGt  >  2:2574187/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TGACCGACGCCGCCCGGGCCGCGGCGCAATCGCTTCTGCACGGCTGAGGCAGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCTGGCAAGATAAAAGGGCAGGGGCTGTCCCCCCCTGCCTTACGATTCCACCACGAATTGCGCGGTGACGATCTGCCAATCCTCGGGGCCGAGGCGTTCGAGCGT  >  NC_014034/914973‑915173

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG