Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,733,287 2 bp→GC 100% coding (309‑310/1128 nt) tgt → queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,733,2870C→G100.0% 1186.9 321S103S (TCC→TCGtgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (166/155):  ref base (0/0):  total (166/155)
*NC_0140341,733,2880G→C100.0% 1180.5 321M104M (GTG→CTG) tgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (166/155):  ref base (0/0):  total (166/155)

Only 100 of 345 total aligned reads displayed.

GCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAA  >  NC_014034/1733193‑1733382
                                                                                              ||                                                                                              
  aTCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCaa                                                                                         >  2:86711/1‑101 (MQ=255)
       gcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGa                                                                                    >  1:337479/1‑101 (MQ=255)
        cgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAc                                                                                   >  2:5552908/1‑101 (MQ=255)
          gCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCg                                                                                 >  1:6920390/1‑101 (MQ=255)
          gCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCg                                                                                 >  2:5657060/1‑101 (MQ=255)
            tGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgag                                                                               >  2:5612335/1‑101 (MQ=255)
             gggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagg                                                                              >  1:6619213/1‑101 (MQ=255)
             gggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagg                                                                              <  1:180642/101‑1 (MQ=255)
               gCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgaggag                                                                            >  1:5928047/1‑101 (MQ=255)
                cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           <  2:6389192/101‑1 (MQ=255)
                cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           <  1:4526324/101‑1 (MQ=255)
                 gggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAggg                                                                          <  2:108879/101‑1 (MQ=255)
                 gggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAggg                                                                          >  1:269056/1‑101 (MQ=255)
                 gggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAggg                                                                          >  1:3116937/1‑101 (MQ=255)
                  ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         >  1:6364192/1‑101 (MQ=255)
                  ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         <  2:4477071/101‑1 (MQ=255)
                  ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         >  1:3469414/1‑101 (MQ=255)
                  ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         >  2:6020427/1‑101 (MQ=255)
                  ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         >  1:2768233/1‑101 (MQ=255)
                    ttGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGt                                                                       >  2:6135488/1‑101 (MQ=255)
                    ttGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGt                                                                       >  1:1911912/1‑101 (MQ=255)
                    ttGCACAAGTTCATGAACGGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTTATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGt                                                                       <  1:3277541/101‑1 (MQ=255)
                     tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCggg                                                                      >  2:2223041/1‑99 (MQ=255)
                     tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      >  1:4286863/1‑101 (MQ=255)
                       cacaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAc                                                                    <  2:2729163/101‑1 (MQ=255)
                        acaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAcc                                                                   <  2:3757184/101‑1 (MQ=255)
                        acaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAcc                                                                   >  2:4283063/1‑101 (MQ=255)
                         caAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCt                                                                  >  2:1542444/1‑101 (MQ=255)
                          aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                 <  1:6039240/101‑1 (MQ=255)
                          aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                 >  1:5406697/1‑101 (MQ=255)
                          aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                 >  1:744301/1‑101 (MQ=255)
                              tCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTcc                                                             <  1:615912/101‑1 (MQ=255)
                              tCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTcc                                                             >  2:5234542/1‑101 (MQ=255)
                              tCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTcc                                                             >  2:2344754/1‑101 (MQ=255)
                              tCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTcc                                                             <  2:342183/101‑1 (MQ=255)
                               cATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCa                                                            <  2:5788/101‑1 (MQ=255)
                               cATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCa                                                            <  1:240677/101‑1 (MQ=255)
                               cATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCa                                                            >  2:814952/1‑101 (MQ=255)
                                atgcccTGGGAGCGCCCGAGCCTGACNGNNNCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAg                                                           <  1:2221372/96‑1 (MQ=255)
                                aTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAg                                                           <  1:2539439/101‑1 (MQ=255)
                                aTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGGGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGGGACCTTTTCCAg                                                           >  1:1023660/1‑101 (MQ=255)
                                 tGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGc                                                          >  1:1464997/1‑101 (MQ=255)
                                  gAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGGGGGCGTGACCTTTTCCAGcc                                                         >  1:5629074/1‑101 (MQ=255)
                                   aaCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCa                                                        >  1:5155414/1‑101 (MQ=255)
                                   aaCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCa                                                        >  2:3170136/1‑101 (MQ=255)
                                     cTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCata                                                      >  1:2147255/1‑101 (MQ=255)
                                      tGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCatat                                                     <  2:7082348/101‑1 (MQ=255)
                                      tGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCatat                                                     >  1:4079337/1‑101 (MQ=255)
                                       gggAGCGCCCGATCCGGACCGCCTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATc                                                    <  2:2882854/101‑1 (MQ=255)
                                        ggAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCg                                                   <  1:2496401/101‑1 (MQ=255)
                                        ggAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCg                                                   <  2:5406425/101‑1 (MQ=255)
                                           gcgcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                >  2:6556944/1‑101 (MQ=255)
                                           gcgcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                <  2:4057746/101‑1 (MQ=255)
                                           gcgcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                >  2:6350303/1‑101 (MQ=255)
                                           gcgcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                >  1:7017190/1‑101 (MQ=255)
                                               ccGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTc                                            >  2:1350082/1‑101 (MQ=255)
                                                 gATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGa                                          >  2:6200691/1‑101 (MQ=255)
                                                 gATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGa                                          >  2:6676295/1‑101 (MQ=255)
                                                 gATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGa                                          >  2:2825501/1‑101 (MQ=255)
                                                  aTCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCggc                                         >  2:332832/1‑99 (MQ=255)
                                                  aTCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGaa                                         >  2:4764429/1‑101 (MQ=255)
                                                   tCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGaaa                                        >  1:1616346/1‑101 (MQ=255)
                                                    ccTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAac                                       <  1:3885612/101‑1 (MQ=255)
                                                     cTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAaca                                      >  1:6439663/1‑101 (MQ=255)
                                                      tGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                     <  1:6201010/101‑1 (MQ=255)
                                                        aCCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCa                                   <  1:3190397/101‑1 (MQ=255)
                                                         ccGCCTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACTTTTTCCAGCCATATCGACGGCTCGAAACACCAt                                  <  1:3708187/101‑1 (MQ=255)
                                                          cGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATc                                 <  1:1249294/101‑1 (MQ=255)
                                                            aCTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTg                               <  1:6598748/101‑1 (MQ=255)
                                                            aCTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTg                               >  2:1806529/1‑101 (MQ=255)
                                                            aCTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTg                               <  2:6468154/101‑1 (MQ=255)
                                                              tCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTc                             >  2:11573/1‑101 (MQ=255)
                                                               cgggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCg                            <  1:6770448/101‑1 (MQ=255)
                                                                gggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGc                           >  1:3599115/1‑101 (MQ=255)
                                                                 ggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGcc                          <  1:2031066/101‑1 (MQ=255)
                                                                 ggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGcc                          <  2:3635608/101‑1 (MQ=255)
                                                                  gcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         >  2:5104173/1‑101 (MQ=255)
                                                                  gcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         <  1:3098081/101‑1 (MQ=255)
                                                                    gggTTTCAGGTTATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGa                       <  2:269052/101‑1 (MQ=255)
                                                                     ggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGac                      >  2:1635059/1‑100 (MQ=255)
                                                                      gTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAAc                     <  1:11574/101‑1 (MQ=255)
                                                                      gTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAAc                     >  2:1698621/1‑101 (MQ=255)
                                                                          cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                 <  1:5553198/101‑1 (MQ=255)
                                                                          cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                 <  1:4997362/101‑1 (MQ=255)
                                                                           aGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCg                <  1:2339719/101‑1 (MQ=255)
                                                                           aGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCg                <  2:3486528/101‑1 (MQ=255)
                                                                             gTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGATCGAAACACCATCTGTCGCCCGAACGCTCGAt              >  2:3899253/1‑101 (MQ=255)
                                                                              tgatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             >  2:2434395/1‑101 (MQ=255)
                                                                              tgatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             >  2:3007158/1‑101 (MQ=255)
                                                                               gatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATgg            >  1:1512764/1‑101 (MQ=255)
                                                                                atgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGga           >  1:1671019/1‑101 (MQ=255)
                                                                                 tgaGCCTTGCTTCGCTGCGTAAGCTGACCGGGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCGCGAACGCTCGATGgag          <  2:468763/101‑1 (MQ=255)
                                                                                   aGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGAt        <  1:4912316/101‑1 (MQ=255)
                                                                                    gCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATc       >  2:6843505/1‑101 (MQ=255)
                                                                                     ctttGCTTCCTGGCGCAACCTGCCCGAGAGGGGCGGGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATcc      <  2:1250662/99‑1 (MQ=255)
                                                                                      cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa     <  1:2125656/101‑1 (MQ=255)
                                                                                      cTTGCTTCGCTGCGCAAGCGGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa     <  2:6604628/101‑1 (MQ=255)
                                                                                       ttGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAg    >  1:5737341/1‑101 (MQ=255)
                                                                                        tGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGa   >  1:7119184/1‑101 (MQ=255)
                                                                                         gCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGaa  >  1:2855464/1‑101 (MQ=255)
                                                                                              ||                                                                                              
GCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAA  >  NC_014034/1733193‑1733382

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG