Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,794,301 2 bp→GC 100% coding (1193‑1194/1770 nt) exaA2 → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,794,3010C→G100.0% 104.4 335A398G (GCG→GGG) exaA2quinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  new base (157/137):  ref base (9/32):  total (166/169)
Fisher's exact test strand distribution p-value = 1.83e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_0140341,794,3020G→C100.0% 103.3 335A398A (GCG→GCCexaA2quinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  new base (157/137):  ref base (8/33):  total (165/170)
Fisher's exact test strand distribution p-value = 4.32e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 343 total aligned reads displayed.

GAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794209‑1794395
                                                                                             ||                                                                                             
 aaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAggg                                                                                       >  2:3977048/1‑101 (MQ=255)
 aaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAggg                                                                                       <  2:4072752/101‑1 (MQ=255)
 aaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAggg                                                                                       <  1:3699148/101‑1 (MQ=255)
  aCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGc                                                                                      >  1:4444189/1‑101 (MQ=255)
    aTCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCca                                                                                    >  1:6813061/1‑101 (MQ=255)
     tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGGTCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   <  1:5104745/101‑1 (MQ=255)
     tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   <  2:3104880/101‑1 (MQ=255)
         cTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGa                                                                               <  2:1721969/101‑1 (MQ=255)
          tGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGAt                                                                              >  1:4010613/1‑101 (MQ=255)
           gggCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATc                                                                             >  2:4415824/1‑101 (MQ=255)
           gggACAAGGGCGTCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATc                                                                             <  2:2300101/101‑1 (MQ=255)
            ggCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCt                                                                            <  1:6110311/101‑1 (MQ=255)
              ccAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCttt                                                                          <  1:1822862/101‑1 (MQ=255)
              ccAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCttt                                                                          <  2:5573724/101‑1 (MQ=255)
               cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTg                                                                         <  2:1142694/101‑1 (MQ=255)
               cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTg                                                                         >  1:4180055/1‑101 (MQ=255)
               cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTg                                                                         <  2:6426304/101‑1 (MQ=255)
                 aGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTggc                                                                       >  2:4278467/1‑99 (MQ=255)
                   ggCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGt                                                                     <  2:4197700/101‑1 (MQ=255)
                   ggCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCgg                                                                     >  1:1602730/1‑100 (MQ=255)
                    gCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTg                                                                    <  2:6080891/101‑1 (MQ=255)
                    gCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTg                                                                    >  1:234960/1‑101 (MQ=255)
                      aTCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGcc                                                                  >  2:6339065/1‑101 (MQ=255)
                       tCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGccc                                                                 >  1:693767/1‑101 (MQ=255)
                        cGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGcagatcgga                                                                >  2:7091376/1‑93 (MQ=255)
                        cGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCt                                                                >  1:1386843/1‑101 (MQ=255)
                         gaggAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               <  1:89553/98‑1 (MQ=255)
                         gATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTc                                                               <  1:1833962/101‑1 (MQ=255)
                           tGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGt                                                             >  1:494911/1‑101 (MQ=255)
                            gAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGtt                                                            >  2:598376/1‑101 (MQ=255)
                             aaaCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTc                                                           >  2:1239829/1‑101 (MQ=255)
                              aaCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTcc                                                          >  1:2918440/1‑101 (MQ=255)
                                ccGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCAAGCAGATCTTTGCCGTGCCATCGTTCCTg                                                        >  2:149030/1‑101 (MQ=255)
                                 cGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTgg                                                       >  2:5395351/1‑101 (MQ=255)
                                 cGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTgg                                                       <  1:6457412/101‑1 (MQ=255)
                                  ggCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTggg                                                      >  2:2729866/1‑101 (MQ=255)
                                   gCCGTCAGATCTACAACGAGGCGAACCGCCCCTGTGCCCCCGGTGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggc                                                     <  2:2114932/101‑1 (MQ=255)
                                    ccGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcg                                                    >  2:3568668/1‑101 (MQ=255)
                                      gTCCGATCTACACCGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGGCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  1:6350522/1‑101 (MQ=255)
                                      gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  <  1:2203685/101‑1 (MQ=255)
                                         cGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAg                                               <  1:3351930/101‑1 (MQ=255)
                                          gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              <  2:87801/101‑1 (MQ=255)
                                          gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              >  1:1179250/1‑101 (MQ=255)
                                          gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              >  1:5226343/1‑101 (MQ=255)
                                          gATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGa                                              <  1:1734382/101‑1 (MQ=255)
                                           aTCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGaa                                             <  1:4124910/101‑1 (MQ=255)
                                           aTCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGaa                                             >  2:4635063/1‑101 (MQ=255)
                                             cTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCTGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACt                                           <  2:896151/101‑1 (MQ=255)
                                             cTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACt                                           >  1:6193170/1‑101 (MQ=255)
                                             cTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACt                                           <  2:6563009/101‑1 (MQ=255)
                                              tACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTg                                          >  2:6943324/1‑101 (MQ=255)
                                               aCAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTgg                                         <  2:4762342/101‑1 (MQ=255)
                                                cAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAG.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGa                                        >  2:3389763/1‑101 (MQ=255)
                                                 aaCGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGAt                                       >  2:2280039/1‑101 (MQ=255)
                                                 aaCGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGAt                                       >  2:3712162/1‑101 (MQ=255)
                                                  aCGAGGAGAACCGCCCCGGTGCCCCCGATGCCTCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATg                                      <  2:5151730/101‑1 (MQ=255)
                                                   cGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGc                                     >  2:1014407/1‑101 (MQ=255)
                                                    gaggagAACCGCCCCGGTGCCCCCGATGCTGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGcc                                    >  1:6582729/1‑101 (MQ=255)
                                                    gaggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGcc                                    <  2:4513315/101‑1 (MQ=255)
                                                     aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCg                                   >  2:4804614/1‑101 (MQ=255)
                                                     aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCg                                   <  1:2023737/101‑1 (MQ=255)
                                                     aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCg                                   >  1:5365715/1‑101 (MQ=255)
                                                     aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCg                                   <  2:6522020/101‑1 (MQ=255)
                                                     aggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCg                                   >  1:6253733/1‑101 (MQ=255)
                                                       gagaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGAt                                 <  1:2960904/101‑1 (MQ=255)
                                                       gagaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGAt                                 >  1:2453254/1‑101 (MQ=255)
                                                         gaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATgg                               >  2:2916962/1‑101 (MQ=255)
                                                         gaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATgg                               >  2:3844046/1‑101 (MQ=255)
                                                          aaCCGCCCCGGTGCCCCCGCTGCCGCCGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTAGTTCCTGGGCGGCAAGAAGTGGATGCAGATGGc                              >  2:1635902/1‑101 (MQ=255)
                                                          aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGc                              >  1:5670015/1‑101 (MQ=255)
                                                           aCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGcc                             <  1:961383/101‑1 (MQ=255)
                                                           aCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGcc                             <  1:1455568/101‑1 (MQ=255)
                                                            ccGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            <  2:6785272/101‑1 (MQ=255)
                                                            ccGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            <  1:1674013/101‑1 (MQ=255)
                                                             cGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTa                           <  2:6161795/101‑1 (MQ=255)
                                                              gCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTAc                          >  2:3450368/1‑101 (MQ=255)
                                                                cccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAg                        >  1:5937171/1‑101 (MQ=255)
                                                                  cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      >  1:3923142/1‑101 (MQ=255)
                                                                  cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  2:4443967/101‑1 (MQ=255)
                                                                  cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      >  2:263796/1‑101 (MQ=255)
                                                                  cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  1:582081/101‑1 (MQ=255)
                                                                   ggTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCa                     <  2:256164/101‑1 (MQ=255)
                                                                    gTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAg                    <  1:4278689/101‑1 (MQ=255)
                                                                        ccccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCa                <  2:4575273/101‑1 (MQ=255)
                                                                         cccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCAc               <  1:4612813/101‑1 (MQ=255)
                                                                           cGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             >  2:1971841/1‑101 (MQ=255)
                                                                           cGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             <  1:2729968/101‑1 (MQ=255)
                                                                            gATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACggg            >  2:5900422/1‑101 (MQ=255)
                                                                             aTGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggc           <  2:2443629/101‑2 (MQ=255)
                                                                              tGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgggcc          <  1:3220980/101‑3 (MQ=255)
                                                                                ccGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCctc        >  1:4817260/1‑101 (MQ=37)
                                                                                ccGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCctc        <  2:3171200/101‑1 (MQ=38)
                                                                                ccGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCctc        <  2:6658576/101‑1 (MQ=38)
                                                                                  gcgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtctt      <  1:4521527/101‑1 (MQ=255)
                                                                                   cgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     <  1:112426/101‑1 (MQ=38)
                                                                                   cgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttc     <  1:3537816/101‑1 (MQ=38)
                                                                                    ggCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttct    <  2:5642600/101‑1 (MQ=38)
                                                                                    ggCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcttct    >  1:100165/1‑101 (MQ=255)
                                                                                    ggCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtcatct    <  2:217428/101‑1 (MQ=38)
                                                                                      ccGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCTCTTCTAt  <  2:1825172/101‑1 (MQ=38)
                                                                                             ||                                                                                             
GAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794209‑1794395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG