Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,950,871 2 bp→GC 100% coding (855‑856/873 nt) garR → 2‑hydroxy‑3‑oxopropionate reductase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,950,8710C→G100.0% 1141.2 311R285R (CGC→CGG) garR2‑hydroxy‑3‑oxopropionate reductase
Reads supporting (aligned to +/- strand):  new base (158/152):  ref base (0/0):  total (158/153)
*NC_0140341,950,8720G→C100.0% 1130.1 311M286M (GTG→CTG) garR2‑hydroxy‑3‑oxopropionate reductase
Reads supporting (aligned to +/- strand):  new base (158/153):  ref base (0/0):  total (158/153)

Only 100 of 321 total aligned reads displayed.

GCCAGCCTGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGCGTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCCG.TTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGTTTTCC  >  NC_014034/1950776‑1950964
                                                                                               ||                                                                                             
gCCAGCCTGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTgcg                                                                                           >  2:6693237/1‑101 (MQ=255)
 ccccccTGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTgcgc                                                                                          <  1:5216294/97‑1 (MQ=255)
     ccTGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTgcgcgcgc                                                                                      >  1:2826667/1‑101 (MQ=255)
       tGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTc                                                                                    <  1:6975900/101‑1 (MQ=255)
       tGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTc                                                                                    >  1:124688/1‑101 (MQ=255)
          cGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGc                                                                                 >  2:4720384/1‑101 (MQ=255)
            gTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTg                                                                               <  2:1169248/101‑1 (MQ=255)
             tCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGa                                                                              <  1:837065/101‑1 (MQ=255)
              cACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAg                                                                             <  2:4110227/101‑1 (MQ=255)
                ccGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCg                                                                           <  2:5337812/101‑1 (MQ=255)
                 cgcgCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCgg                                                                          <  1:6923971/101‑1 (MQ=255)
                 cgcgCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCgg                                                                          >  1:4029213/1‑101 (MQ=255)
                  gcgcTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCggg                                                                         <  1:5142805/101‑1 (MQ=255)
                   cgcTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGa                                                                        >  2:6425731/1‑101 (MQ=255)
                     cTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAgg                                                                      <  2:335247/101‑1 (MQ=255)
                      tGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAggg                                                                     <  1:2667862/101‑1 (MQ=255)
                       ggTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAgggg                                                                    >  2:3802795/1‑101 (MQ=255)
                        gTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAgaaaa                                                                   <  2:1300725/101‑5 (MQ=255)
                         tCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAgggggg                                                                  <  1:252673/101‑1 (MQ=255)
                         tCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAgggggg                                                                  >  2:3928343/1‑101 (MQ=255)
                          cGATCAGTTCTAACAGGATGGGCAGAATCTGGGCGGCGGGCACGGGGACCCCTCGAGCCTGATCGCGCGTCTGCGCGCGCTCGGCTGAGCGGGAGGGGGgc                                                                 <  2:2267835/101‑1 (MQ=255)
                          cGATCAGTTCTAAAAGGATGTGCAGAAGCTGGGCGGCTGGCGCTGGGACACCTCGAGCCTGAGCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGgc                                                                 >  1:5101435/1‑101 (MQ=255)
                           gATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGgcg                                                                <  1:3112547/101‑1 (MQ=255)
                             tCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCt                                                              >  1:3374926/1‑101 (MQ=255)
                              cAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTg                                                             >  1:6343508/1‑101 (MQ=255)
                              cAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTg                                                             >  1:7064870/1‑101 (MQ=255)
                              cAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTg                                                             >  2:2701199/1‑101 (MQ=255)
                              cAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTg                                                             >  2:6796630/1‑101 (MQ=255)
                                  tCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccc                                                         >  1:195273/1‑101 (MQ=255)
                                  tCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccc                                                         <  2:3475181/101‑1 (MQ=255)
                                  tCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccc                                                         >  1:4490103/1‑101 (MQ=255)
                                   cTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGccccc                                                        <  1:5665734/101‑1 (MQ=255)
                                    tACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccg                                                       >  1:6699125/1‑100 (MQ=255)
                                       aaGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGGGCTGcccccggct                                                    >  2:1419659/1‑97 (MQ=255)
                                       aaGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggct                                                    >  2:3665002/1‑97 (MQ=255)
                                        aGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggctt                                                   <  1:2430036/101‑6 (MQ=255)
                                         ggATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggcttc                                                  <  2:5075895/101‑7 (MQ=255)
                                          gATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggcttcg                                                 <  1:2248828/101‑8 (MQ=255)
                                          gATGGGCAGAAGCTGGGCGGCGGGCGCCGGGACACCCCGAGCCTGATCGCGCGGCGGCGCGCGCTCTGCGAAGCGGGAGGGGGGCGCTGcccccggcttcg                                                 <  2:2086085/101‑8 (MQ=255)
                                             gtgCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccc                                              >  2:2037676/1‑101 (MQ=255)
                                              tgCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGcccc                                             >  1:681593/1‑101 (MQ=255)
                                               gCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccccc                                            <  1:5356920/101‑1 (MQ=255)
                                               gCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccccc                                            >  1:16274/1‑101 (MQ=255)
                                                cAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGGTGCCCCCGGCTTCGcccccc                                           >  1:3566757/1‑101 (MQ=255)
                                                 aGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccccccc                                          <  1:5548918/101‑1 (MQ=255)
                                                 aGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccccccc                                          >  2:4318729/1‑101 (MQ=255)
                                                 aGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccccccc                                          <  2:4621010/101‑1 (MQ=255)
                                                 aGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCCccccccc                                          >  2:6217524/1‑101 (MQ=255)
                                                 aGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCCTCGccccccc                                          >  2:5701616/1‑101 (MQ=255)
                                                    aGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCggg                                       >  2:7109596/1‑101 (MQ=255)
                                                      cTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGat                                     <  2:2472038/101‑1 (MQ=255)
                                                      cTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGat                                     <  2:2239110/101‑1 (MQ=255)
                                                      cTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGat                                     <  2:16273/101‑1 (MQ=255)
                                                       tGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGata                                    >  1:3837528/1‑101 (MQ=255)
                                                        gggCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGatat                                   >  1:1121473/1‑101 (MQ=255)
                                                        gggCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGatat                                   >  2:2967307/1‑101 (MQ=255)
                                                        gggCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGatat                                   >  1:158093/1‑101 (MQ=255)
                                                         ggcggcGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATAtt                                  >  1:2771145/1‑101 (MQ=255)
                                                           cggcggGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTg                                <  2:1261534/101‑1 (MQ=38)
                                                           cggcggGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTg                                <  1:6173712/101‑1 (MQ=38)
                                                            ggcgggcgCTGGGACCCCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGc                               <  1:538071/101‑1 (MQ=38)
                                                            ggcgggcgCTGGGACCCCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGc                               <  2:2755921/101‑1 (MQ=38)
                                                             gcgggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCACCCCCCCGGGATATTTGcc                              <  1:6275350/101‑1 (MQ=255)
                                                               gggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGcccc                            >  2:4255316/1‑101 (MQ=38)
                                                               gggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGcccc                            >  2:4858696/1‑101 (MQ=38)
                                                               gggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGcccc                            <  2:4543042/101‑1 (MQ=38)
                                                                ggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCa                           <  1:1788108/101‑1 (MQ=38)
                                                                  cgcTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAg                         <  1:5844900/101‑1 (MQ=38)
                                                                   gcTGGGCCACCTGGAGCCGGATCGCGCGGCTGCGCGCGCTCGCCTGAGCGGGAGGGGGGCGCTCCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAgg                        <  2:1747145/101‑1 (MQ=25)
                                                                    cTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAggg                       >  1:4902493/1‑100 (MQ=38)
                                                                    cTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAggg                       >  1:4869482/1‑100 (MQ=38)
                                                                    cTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGt                       <  2:2795467/101‑1 (MQ=38)
                                                                     tGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTg                      >  1:6430351/1‑101 (MQ=38)
                                                                      gggACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGa                     <  1:4220717/101‑1 (MQ=38)
                                                                      gggACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGa                     <  2:6314711/101‑1 (MQ=38)
                                                                         acacCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAg                  >  1:2875686/1‑101 (MQ=37)
                                                                         acacCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAg                  >  1:5990437/1‑101 (MQ=37)
                                                                         acacCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAg                  <  1:6565008/101‑1 (MQ=38)
                                                                             cTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCcgc              <  2:5551104/101‑1 (MQ=38)
                                                                             cTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGCGCGCTGCCCCCGGCTTCCCCCCCCCGGGGTGGTTGGCCAAAGGTGAAAGcctc              >  2:5642188/1‑99 (MQ=255)
                                                                              tCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCcgcg             >  2:6533841/1‑101 (MQ=37)
                                                                              tCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCcgcg             <  1:1522856/101‑1 (MQ=38)
                                                                                gAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGcgac           >  1:2156020/1‑101 (MQ=37)
                                                                                gAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGcgac           <  2:3216044/101‑1 (MQ=38)
                                                                                gAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGcgac           >  2:3044698/1‑101 (MQ=37)
                                                                                 ccgatcttCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGcgacg          <  2:5932399/94‑1 (MQ=37)
                                                                                 aGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGAGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGcgacg          <  1:6015702/101‑1 (MQ=37)
                                                                                    cTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGt       >  1:1014110/1‑101 (MQ=38)
                                                                                     tGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGccagatcggaag      >  1:1184622/1‑91 (MQ=25)
                                                                                     tGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtt      <  2:5214329/101‑1 (MQ=38)
                                                                                     tGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtt      >  1:4403461/1‑101 (MQ=38)
                                                                                     tGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCACCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtt      >  2:888910/1‑101 (MQ=37)
                                                                                      gATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCCAGGTGAAAGCCGCGACGAGttt     >  2:400320/1‑101 (MQ=38)
                                                                                      gATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGttt     <  2:4428303/101‑1 (MQ=38)
                                                                                      gATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGttt     <  1:6516867/101‑1 (MQ=38)
                                                                                      gATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGttt     <  1:3142876/101‑1 (MQ=38)
                                                                                      gATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGttt     <  1:137076/101‑1 (MQ=38)
                                                                                       aTCGCGCGGCTGCGCGCGCTTGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtttt    <  2:2599813/101‑1 (MQ=37)
                                                                                       aTCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtttt    <  2:4952550/101‑1 (MQ=38)
                                                                                        tCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAgatcgg   >  1:5932703/1‑96 (MQ=37)
                                                                                               ||                                                                                             
GCCAGCCTGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGCGTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCCG.TTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGTTTTCC  >  NC_014034/1950776‑1950964

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG