Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,053,078 2 bp→GC 100% coding (4156‑4157/6198 nt) RCAP_rcc01900 ← hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,053,0780C→G100.0% 1000.7 271R1386P (CGG→CCG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (137/133):  ref base (0/0):  total (138/133)
*NC_0140342,053,0790G→C100.0% 1000.6 271R1386G (CGG→GGG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (138/133):  ref base (0/0):  total (138/133)

Only 100 of 287 total aligned reads displayed.

GGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCAC  >  NC_014034/2052983‑2053172
                                                                                               ||                                                                                             
ggATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCg                                                                                           >  1:104226/1‑101 (MQ=255)
ggATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCg                                                                                           <  1:244920/101‑1 (MQ=255)
  aTGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGcc                                                                                         >  2:1897173/1‑101 (MQ=255)
   tGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCg                                                                                        <  1:4191762/101‑1 (MQ=255)
    gTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGa                                                                                       >  2:1715166/1‑101 (MQ=255)
     tCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAg                                                                                      <  2:5482638/101‑1 (MQ=255)
     tCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAg                                                                                      <  2:5161209/101‑1 (MQ=255)
        ttGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGAt                                                                                   <  1:339346/101‑1 (MQ=255)
        ttGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGAt                                                                                   >  2:4172885/1‑101 (MQ=255)
          gCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATgg                                                                                 >  2:1631243/1‑101 (MQ=255)
            ccccgccgcCAAGGATCCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgtg                                                                               <  2:3764658/98‑1 (MQ=38)
             cgcgCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgtgt                                                                              <  1:5496882/101‑1 (MQ=255)
              gcgcCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTc                                                                             <  2:870062/101‑1 (MQ=255)
               tccgatctaGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCg                                                                            <  2:1207517/93‑1 (MQ=38)
                gccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGt                                                                           <  1:5082387/101‑1 (MQ=255)
                gccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGAGGGTGTGGt                                                                           >  2:2275923/1‑101 (MQ=38)
                  ccccAAGAATGCTTACGTCAACCACCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTTTctatg                                                                         <  2:328035/99‑5 (MQ=21)
                    ccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGcc                                                                       <  1:3558996/101‑1 (MQ=255)
                     cAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGccc                                                                      <  2:3913243/101‑1 (MQ=255)
                     cAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGccc                                                                      >  1:7032172/1‑101 (MQ=255)
                     cAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGccc                                                                      >  2:4925863/1‑101 (MQ=255)
                      aaGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCt                                                                     <  2:6687107/101‑1 (MQ=255)
                        ggATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcc                                                                   >  1:313120/1‑101 (MQ=255)
                        ggATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcc                                                                   >  2:6963602/1‑101 (MQ=255)
                          ttGCTGTCGTCATGCAGCCCGCCGGGTATGCTGTCATTGCCCGCGCCACCGTCCACCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcccc                                                                 <  1:895601/100‑1 (MQ=37)
                          aTGCTGTCGTCATCCGGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcccc                                                                 <  2:6544438/101‑1 (MQ=255)
                             cTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGcc                                                              >  1:1276559/1‑101 (MQ=255)
                              tGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                             >  1:2566106/1‑101 (MQ=255)
                              ggtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                             <  2:855064/100‑1 (MQ=255)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                            <  1:5807537/101‑1 (MQ=255)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                            >  1:6145598/1‑101 (MQ=255)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                            >  2:7069076/1‑101 (MQ=255)
                               gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                            >  1:1976242/1‑101 (MQ=255)
                                tctgccgccAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAg                                                           <  2:6158931/94‑1 (MQ=38)
                                    cATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAcc                                                       >  2:2080734/1‑101 (MQ=255)
                                       ccAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAg                                                    <  2:2407144/101‑1 (MQ=255)
                                        cAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGt                                                   <  2:130729/101‑1 (MQ=255)
                                         cgCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                  <  1:3334403/100‑1 (MQ=255)
                                          gCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCa                                                 <  1:2201774/101‑1 (MQ=255)
                                          gCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCa                                                 <  2:1783421/101‑1 (MQ=255)
                                          gCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCa                                                 <  1:5895512/101‑1 (MQ=255)
                                           cccGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCAt                                                <  2:3709481/101‑1 (MQ=255)
                                           cccGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCCAGGCAt                                                >  2:5989516/1‑101 (MQ=38)
                                             cgccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCg                                              >  1:1782977/1‑101 (MQ=255)
                                              gccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGc                                             <  2:2867504/101‑1 (MQ=255)
                                                cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                           <  1:1897245/101‑1 (MQ=255)
                                                cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                           <  2:5677530/101‑1 (MQ=255)
                                                   tGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCgcg                                        >  2:6357385/1‑99 (MQ=255)
                                                   tGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGac                                        >  2:6470858/1‑100 (MQ=255)
                                                    gATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGc                                       >  1:4415615/1‑101 (MQ=255)
                                                    gATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGCCATCGCCCGAGc                                       >  1:7115646/1‑101 (MQ=255)
                                                      tGCTGTCATTGCCTGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGccc                                     >  2:6774374/1‑101 (MQ=255)
                                                       gCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCg                                    <  1:6415824/101‑1 (MQ=255)
                                                         tGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCCGCACCGAGTCATCGCTCGCGCGCGcc                                  >  2:280205/1‑101 (MQ=37)
                                                         tGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGcc                                  <  1:5317071/101‑1 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGccc                                 >  1:1111076/1‑100 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCt                                 >  2:745391/1‑101 (MQ=255)
                                                           tCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                <  1:1024287/101‑1 (MQ=255)
                                                           tCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                <  1:6237378/101‑1 (MQ=255)
                                                           tCATTGCCCGCCCCACCGTCCAGCGTGTCATCACCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                <  1:4944006/101‑1 (MQ=38)
                                                            cATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcg                               >  2:3161727/1‑101 (MQ=255)
                                                             aTTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcga                              >  1:6613414/1‑101 (MQ=255)
                                                               tGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatc                            <  1:1665120/101‑1 (MQ=255)
                                                                gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTTCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                           >  1:210465/1‑101 (MQ=255)
                                                                gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                           <  2:2709796/101‑1 (MQ=255)
                                                                 cccGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcga                          >  1:5048294/1‑101 (MQ=255)
                                                                  ccGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCTATCGaa                         >  1:6376832/1‑101 (MQ=255)
                                                                   cgcgCCCCCGTCCGGCGTGTCATCGCCCCCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAt                        <  1:6515759/101‑1 (MQ=255)
                                                                    gcgcCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                       <  2:3042806/101‑1 (MQ=255)
                                                                    gcgcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                       >  1:3743567/1‑101 (MQ=255)
                                                                     cgcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcg                      >  1:4016350/1‑101 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGCTCGAAtcgc                     >  2:2913950/1‑100 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgt                     <  1:1180333/101‑1 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgt                     >  2:5517841/1‑101 (MQ=255)
                                                                        ccccGTCCCGCGTCTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTCCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtcg                   <  1:3652671/99‑1 (MQ=255)
                                                                        cACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtcg                   >  2:5021641/1‑101 (MQ=255)
                                                                          ccGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGcc                 >  1:1925821/1‑101 (MQ=255)
                                                                          ccGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTAGcc                 <  1:6829368/101‑1 (MQ=255)
                                                                           cGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCg                >  1:2694702/1‑101 (MQ=255)
                                                                           cGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCg                <  1:3833844/101‑1 (MQ=255)
                                                                            gTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCgg               >  2:5758118/1‑101 (MQ=255)
                                                                            gTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCgg               >  1:2942444/1‑101 (MQ=255)
                                                                             tCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCggg              >  2:5065516/1‑100 (MQ=255)
                                                                             gccAGCGTGTCATCGCCCGCTTCGCCGAGGATGGGGTCGTTGCCCTCCCCGCCGAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGt              <  2:1390819/100‑1 (MQ=255)
                                                                              ccAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGCGCCCGCCTCGATCGAATCGTGGCCGGTg             >  2:5717736/1‑101 (MQ=255)
                                                                              ccAGAGTGTCATCGCCCGCTTCGCCGAGGATGGGGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCTCGAGACCGCCTCGACCGAATCGTCGcctaag             >  2:438908/1‑97 (MQ=255)
                                                                               cAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGc            >  1:5655410/1‑101 (MQ=255)
                                                                               cAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGCGc            >  1:6473743/1‑101 (MQ=255)
                                                                               cAGCGTGTCATCGCCCGCTGCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGc            <  2:4605428/101‑1 (MQ=255)
                                                                                aGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGcc           >  2:3200086/1‑101 (MQ=255)
                                                                                 gCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGccc          <  1:695009/101‑1 (MQ=255)
                                                                                 gCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGccc          >  2:4631104/1‑101 (MQ=255)
                                                                                 gCGTGTCATCGCCCGCGTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGccc          <  2:3527261/101‑1 (MQ=255)
                                                                                  cGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcg         <  2:1030816/101‑1 (MQ=255)
                                                                                   gtgtCCTCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgc        <  2:5890153/101‑1 (MQ=255)
                                                                                   gtgtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgc        >  2:3959271/1‑101 (MQ=255)
                                                                                   gtgtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgc        <  2:3439724/101‑1 (MQ=255)
                                                                                    tgtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgcg       >  1:2831224/1‑101 (MQ=255)
                                                                                       caccGCCCGCTTCGCCGGGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTc    <  1:4534623/98‑1 (MQ=255)
                                                                                       cATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACGGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTc    <  2:4298292/101‑1 (MQ=255)
                                                                                               ||                                                                                             
GGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCAC  >  NC_014034/2052983‑2053172

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG