Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,157,926 2 bp→AC 100% intergenic (‑298/‑483) RCAP_rcc02004 ← / → RCAP_rcc02005 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,157,9260C→A100.0% 134.3 333intergenic (‑298/‑484)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (117/170):  ref base (42/2):  total (160/173)
Fisher's exact test strand distribution p-value = 5.85e-13
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_0140342,157,9270G→C100.0% 114.6 334intergenic (‑299/‑483)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (118/172):  ref base (42/2):  total (160/174)
Fisher's exact test strand distribution p-value = 5.34e-13
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

CTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGTCT  >  NC_014034/2157834‑2158022
                                                                                             ||                                                                                               
ctcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCg                                                                                          >  2:2574357/1‑101 (MQ=255)
ctcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCg                                                                                          <  1:4721917/101‑1 (MQ=255)
ctcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCg                                                                                          <  2:2189176/101‑1 (MQ=255)
 tcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCgg                                                                                         <  2:1725156/101‑1 (MQ=255)
  cccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGt                                                                                        <  2:6920293/101‑1 (MQ=255)
   ccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGtt                                                                                       <  2:5758660/101‑1 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                      <  2:69519/101‑1 (MQ=255)
    cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                      <  2:6564548/101‑1 (MQ=255)
     cgCATCCCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCa                                                                                     <  2:1196891/100‑1 (MQ=37)
     aGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCa                                                                                     <  1:3257838/101‑1 (MQ=255)
        aTACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTc                                                                                  >  1:3069435/1‑101 (MQ=255)
        aTACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTCGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTc                                                                                  >  2:948392/1‑101 (MQ=38)
         tACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTcc                                                                                 >  2:4753654/1‑101 (MQ=255)
          aCCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccg                                                                                >  1:1255673/1‑101 (MQ=255)
             agaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccgccg                                                                             >  1:4261798/3‑101 (MQ=255)
              aaCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTTATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgt                                                                            <  1:4209494/101‑1 (MQ=38)
               aCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtc                                                                           >  2:4540227/1‑101 (MQ=255)
               aCCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtc                                                                           <  2:2850335/101‑1 (MQ=255)
                   cTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCt                                                                       >  2:1972464/1‑101 (MQ=255)
                     ggCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTg                                                                     <  2:5968448/101‑1 (MQ=255)
                       cGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGc                                                                   <  2:1538096/101‑1 (MQ=255)
                         aCCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCgg                                                                 >  2:3390732/1‑101 (MQ=255)
                         aCCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCgg                                                                 >  2:732395/1‑101 (MQ=255)
                              cAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACg                                                            >  2:4388893/1‑101 (MQ=255)
                               aGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGt                                                           <  1:2772678/101‑1 (MQ=255)
                               aGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGt                                                           >  2:1323175/1‑101 (MQ=255)
                               aGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGCCGGTGACgg                                                           >  1:7101112/1‑100 (MQ=38)
                                    ctgTCCGCCGTCGGCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                      <  2:497107/99‑1 (MQ=38)
                                    cAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                      <  1:5442422/101‑1 (MQ=255)
                                    cAGGCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                      <  2:3095414/101‑1 (MQ=37)
                                      gTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCAc                                                    >  2:1480526/1‑101 (MQ=255)
                                        ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGCCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                  <  1:3699944/101‑1 (MQ=38)
                                         cgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTa                                                 <  1:2996181/101‑1 (MQ=255)
                                         cgccgTCGTCTTGGCGGTGATGTGATGCACAGCAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTa                                                 <  2:3896654/101‑1 (MQ=37)
                                          gccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaa                                                <  1:1671607/101‑1 (MQ=255)
                                          gccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaa                                                <  1:822079/101‑1 (MQ=255)
                                            cgtcgtCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAAc                                              <  1:4226555/101‑1 (MQ=255)
                                            cgtcgtCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAAc                                              >  2:4402658/1‑101 (MQ=255)
                                            cgtcgtCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAAc                                              >  1:1373934/1‑101 (MQ=255)
                                             gtcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACa                                             <  1:4069941/101‑1 (MQ=255)
                                             gtcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACa                                             <  2:572052/101‑1 (MQ=255)
                                                gtcTTGGCGGTGCTGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                          >  1:5094609/1‑101 (MQ=38)
                                                gtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                          <  1:1002718/101‑1 (MQ=255)
                                                 tcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAagag                                         <  2:699255/101‑1 (MQ=255)
                                                 tcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAagag                                         <  1:1339575/101‑1 (MQ=255)
                                                 tcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAagag                                         <  1:5145862/101‑1 (MQ=255)
                                                  cTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                        <  1:2718644/101‑1 (MQ=255)
                                                  cTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                        >  2:4453847/1‑101 (MQ=255)
                                                  cTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                        >  1:5334141/1‑101 (MQ=255)
                                                    tGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGtt                                      >  1:4704694/1‑101 (MQ=255)
                                                    tGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGGGACGGGAGGGACCTAAAAAAGGGGtc                                      >  2:3293093/1‑100 (MQ=37)
                                                     ggCGGTGATGTGATGCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTc                                     >  2:4294521/1‑101 (MQ=255)
                                                       cGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTa                                   <  1:2401667/101‑1 (MQ=255)
                                                        ggTGATGTGATGCACAGGAGAAGCCGATTAAAATC.ATCGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTAc                                  <  1:354502/101‑1 (MQ=37)
                                                         gtgatgtgatGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                                 <  2:3842930/101‑1 (MQ=255)
                                                         gtgatgtgatGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                                 <  2:6835364/101‑1 (MQ=255)
                                                          tgatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGa                                >  1:9355/1‑101 (MQ=255)
                                                          tgatgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGa                                >  1:5907089/1‑101 (MQ=255)
                                                           gatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                               >  2:85535/1‑101 (MQ=255)
                                                            atgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                              >  2:2389352/1‑101 (MQ=255)
                                                            atgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                              <  1:2579570/101‑1 (MQ=255)
                                                             tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                             <  1:1588500/101‑1 (MQ=255)
                                                             tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                             <  2:1104846/101‑1 (MQ=255)
                                                              gtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGcc                            <  1:1121564/101‑1 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                           >  2:5450395/1‑101 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                           >  1:6670272/1‑101 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                           >  1:502959/1‑101 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                           <  1:2199953/101‑1 (MQ=255)
                                                               tgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                           <  1:4489254/101‑1 (MQ=255)
                                                                gatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGa                          >  2:107010/1‑101 (MQ=255)
                                                                   gCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAg                       <  2:5039617/101‑1 (MQ=255)
                                                                    cacaGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAgg                      >  1:4897924/1‑101 (MQ=255)
                                                                    cacaGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAgg                      >  1:2097110/1‑101 (MQ=255)
                                                                    cacaGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAgg                      <  2:3658531/101‑1 (MQ=255)
                                                                     acaGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAggg                     <  1:4605374/101‑1 (MQ=255)
                                                                       aGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGt                   >  2:6268977/1‑101 (MQ=255)
                                                                        ggAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGtt                  <  2:2745649/101‑1 (MQ=255)
                                                                        ggAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGtt                  <  1:1906078/101‑1 (MQ=255)
                                                                         gagagaCCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTc                 >  1:6127902/1‑101 (MQ=255)
                                                                         gagagaCCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTc                 >  1:6493917/1‑101 (MQ=255)
                                                                          agagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCa                >  1:2477594/1‑101 (MQ=255)
                                                                          agagaCCGAGGACAATC.AACGGCTTCGGTTCAGTCCTCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCa                <  1:6942442/101‑1 (MQ=37)
                                                                           gagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAg               <  2:1645464/101‑1 (MQ=255)
                                                                           gagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAg               <  2:5520403/101‑1 (MQ=255)
                                                                           gagaCCGATGAAAATC.AACAGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAg               <  1:6822492/101‑1 (MQ=38)
                                                                            agaCCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGt              >  1:6453263/1‑101 (MQ=255)
                                                                            agaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGt              <  2:561423/101‑1 (MQ=255)
                                                                             gaCCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTc             >  2:5855169/1‑101 (MQ=255)
                                                                             gaCCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTc             >  2:3480492/1‑101 (MQ=255)
                                                                             gaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTc             <  2:2093338/101‑1 (MQ=255)
                                                                              aCCGATGAAAATC.AACTGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTcc            <  2:5233263/101‑1 (MQ=38)
                                                                              aCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTcc            >  2:2466807/1‑101 (MQ=255)
                                                                              aCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTcc            <  2:4897680/101‑1 (MQ=255)
                                                                                cGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgc          <  1:603565/101‑1 (MQ=255)
                                                                                    gAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtc      <  1:5546127/101‑1 (MQ=255)
                                                                                    gAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtc      <  1:5817942/101‑1 (MQ=255)
                                                                                    gAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtc      >  1:2621824/1‑101 (MQ=255)
                                                                                    gAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtc      >  1:3866890/1‑101 (MQ=255)
                                                                                     aaaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcg     >  1:3774746/1‑101 (MQ=255)
                                                                                        aTC.ACCGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGTCt  <  1:5287120/101‑1 (MQ=255)
                                                                                             ||                                                                                               
CTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGTCT  >  NC_014034/2157834‑2158022

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG