Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,289 2 bp→AT 100% coding (87‑88/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2890T→A100.0% 1029.5 271T30S (ACA→TCA) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (157/113):  ref base (0/0):  total (158/113)
*NC_0140342,177,2900A→T100.0% 1053.0 266C29* (TGT→TGArbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (156/110):  ref base (0/0):  total (156/110)

Only 100 of 273 total aligned reads displayed.

CATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTT..G..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.A.GCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCC.AACAGGGCCAG.TTGCGCATC  >  NC_014034/2177202‑2177374
                                                                                              ||                                                                                          
cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGAtcagacag                                                                                     <  1:3148239/101‑1 (MQ=255)
cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGAtcagacag                                                                                     <  2:3363886/101‑1 (MQ=255)
 aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAgg                                                                                    >  2:141270/1‑101 (MQ=255)
  tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGc                                                                                   >  2:5629057/1‑101 (MQ=255)
  tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGc                                                                                   >  2:5559158/1‑101 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGccc                                                                                 >  2:2328688/1‑101 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGccc                                                                                 <  2:3898558/101‑1 (MQ=255)
     tctgAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGcccc                                                                                <  2:1281417/98‑1 (MQ=255)
     gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGcccc                                                                                <  2:3620723/101‑1 (MQ=255)
     gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGcccc                                                                                <  2:5142684/101‑1 (MQ=255)
       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCgg                                                                              >  2:6734748/1‑101 (MQ=255)
       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCgg                                                                              <  1:5487314/101‑1 (MQ=255)
        gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt                                                                             >  1:4788224/1‑101 (MQ=255)
        gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt                                                                             >  1:5395870/1‑101 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGtt                                                                            >  1:4472679/4‑101 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGtt                                                                            >  1:3111077/4‑101 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGtt                                                                            <  1:2636327/98‑1 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                           <  2:569019/99‑1 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGAACCTTCCTTCATCTTGACGAAAAAGGGGTTGGG..GTCGGTCTTGGTGATCAGACAGGCCCCgcgtt                                                                           >  2:6928645/3‑97 (MQ=255)
           agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTc                                                                          <  1:4986210/100‑1 (MQ=255)
           agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTc                                                                          <  1:4608615/100‑1 (MQ=255)
            gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                                         <  2:4878979/101‑1 (MQ=255)
              cccACCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                                                       <  1:3336668/100‑1 (MQ=255)
               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                                                      >  1:989649/1‑101 (MQ=255)
                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                     >  2:6516097/1‑101 (MQ=255)
                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                     <  1:3727032/101‑1 (MQ=255)
                  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                                                  <  1:758724/101‑2 (MQ=255)
                    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                                                <  1:1158659/101‑4 (MQ=255)
                    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCAG.atcg                                                                <  2:5866813/101‑4 (MQ=255)
                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                                               <  2:3041653/101‑5 (MQ=255)
                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                                               <  2:2448632/101‑5 (MQ=255)
                        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.gccgcc                                                            <  1:1034807/101‑1 (MQ=255)
                        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCAGCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.gccgcc                                                            >  1:5014790/1‑101 (MQ=255)
                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGccc                                                           <  2:3469838/101‑1 (MQ=255)
                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGcccc                                                          <  2:6804421/101‑1 (MQ=255)
                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGcccc                                                          >  2:4588810/1‑101 (MQ=255)
                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCg                                                         <  1:1086559/101‑1 (MQ=255)
                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCg                                                         >  1:5445054/1‑101 (MQ=255)
                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCg                                                         <  1:3386359/101‑1 (MQ=255)
                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCg                                                         <  2:3228103/101‑1 (MQ=255)
                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCgg                                                        >  1:2107203/1‑101 (MQ=255)
                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCgg                                                        >  2:2817737/1‑101 (MQ=255)
                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCgg                                                        >  1:4188001/1‑101 (MQ=255)
                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCgg                                                        >  2:3118737/1‑101 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCa                                                     >  2:4025587/1‑101 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCa                                                     >  1:5291423/1‑101 (MQ=255)
                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAgc                                                   >  2:379502/1‑101 (MQ=255)
                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAgc                                                   <  2:6327953/101‑1 (MQ=255)
                                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAgcg                                                  <  2:5456357/101‑1 (MQ=255)
                                   tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAgcgc                                                 <  1:2752451/101‑1 (MQ=255)
                                    ttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGcgcg                                                >  1:3246352/1‑101 (MQ=255)
                                    ttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGcgcg                                                >  2:4543823/1‑101 (MQ=255)
                                     tGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCgg                                               >  2:4199414/1‑101 (MQ=255)
                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGaa                                             <  2:903212/101‑1 (MQ=255)
                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.ACGCCGCCCCGGCCAGCGCGGaa                                             >  2:5218636/1‑101 (MQ=255)
                                        cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGaaa                                            >  1:1339225/1‑101 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGaaaa                                           >  1:2850857/1‑101 (MQ=255)
                                          aaGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAAc                                          <  2:1854270/101‑1 (MQ=255)
                                          aaGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAAc                                          >  2:4336992/1‑101 (MQ=255)
                                          aaGCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAAc                                          >  1:6697909/1‑101 (MQ=255)
                                            gCACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGc                                        >  2:2727564/1‑101 (MQ=255)
                                             cACCTTCCTTCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGcc                                       >  1:5751107/1‑101 (MQ=255)
                                               ccttccttCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                    >  2:4713858/1‑101 (MQ=255)
                                               ccttccttCATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                    >  2:3416091/1‑101 (MQ=255)
                                                cttccttcATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCaag                                    >  1:3890808/1‑99 (MQ=255)
                                                cttccttcATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                   >  1:1646559/1‑101 (MQ=255)
                                                 ttccttcATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                  <  2:3986841/101‑2 (MQ=255)
                                                  tccttcATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCTA.gggc                                 >  1:3833421/1‑99 (MQ=255)
                                                  tccttcATCTTGACGAAAAACGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCacggc                                  <  1:6840917/101‑5 (MQ=255)
                                                    cttcATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                               <  1:6085137/101‑5 (MQ=255)
                                                     ttcATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                              <  2:6004839/101‑6 (MQ=255)
                                                     ttcATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                              <  1:3192140/101‑6 (MQ=255)
                                                       cATCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                             >  1:5914718/1‑101 (MQ=255)
                                                        aTCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                           <  1:728544/101‑1 (MQ=255)
                                                        aTCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                           >  2:617625/1‑101 (MQ=255)
                                                         tCTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                          >  1:1997928/1‑101 (MQ=255)
                                                          cTTGACGAAAAAGGGGTT..GGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                         <  1:2372150/101‑1 (MQ=255)
                                                                       aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGcc              >  2:6128574/11‑101 (MQ=255)
                                                                       aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGcc              <  1:141273/91‑1 (MQ=255)
                                                                       aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGcc              <  2:1182343/91‑1 (MQ=255)
                                                                        ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCa             <  2:2139383/92‑1 (MQ=255)
                                                                        ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCa             <  1:18285/92‑1 (MQ=255)
                                                                           gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tt         >  2:5103085/7‑101 (MQ=255)
                                                                            ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.ttt        <  1:2443297/96‑2 (MQ=255)
                                                                            ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.ttt        >  1:61377/6‑100 (MQ=255)
                                                                            ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.ttt        >  2:4459459/6‑100 (MQ=255)
                                                                            ttggtgccggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.ATCAGGGCCAG.ttt        >  1:7003396/8‑100 (MQ=255)
                                                                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGCGG.CCCCAAACAGGGCCAG.tttg       >  2:3334798/5‑99 (MQ=255)
                                                                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttg       >  1:1933114/5‑99 (MQ=255)
                                                                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttg       >  2:2392109/5‑99 (MQ=255)
                                                                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttg       >  1:1749347/5‑99 (MQ=255)
                                                                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttg       <  1:4297094/97‑3 (MQ=255)
                                                                              ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttgc      >  1:6430006/4‑98 (MQ=255)
                                                                              ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttgc      >  2:6226432/4‑98 (MQ=255)
                                                                               gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttgcg     >  1:3127068/3‑97 (MQ=255)
                                                                                tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttgcgc    >  2:1030149/2‑96 (MQ=255)
                                                                                  tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAGTTTGCGcat   >  1:903370/1‑101 (MQ=255)
                                                                                  tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAG.tttgcccat  >  1:2189961/1‑94 (MQ=255)
                                                                                  tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCACAGGCCCCC.AACAGGGCCAGTTTGCGcat   <  1:6285100/101‑1 (MQ=255)
                                                                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAA.GCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCC.AACAGGGCCAGTTTGCGcatc  >  1:1543496/1‑101 (MQ=255)
                                                                                              ||                                                                                          
CATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTT..G..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.A.GCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCC.AACAGGGCCAG.TTGCGCATC  >  NC_014034/2177202‑2177374

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG