Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,185 2 bp→GC 100% coding (874‑875/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1850C→G100.0% 1148.1 310R292G (CGC→GGC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (159/151):  ref base (0/0):  total (159/151)
*NC_0140342,180,1860G→C100.0% 1156.7 310R292P (CGC→CCC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (159/150):  ref base (0/0):  total (159/151)

Only 100 of 315 total aligned reads displayed.

ATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCG.AACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACC  >  NC_014034/2180093‑2180283
                                                                                                ||                                                                                                 
aTGCGCCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAg                                                                                               <  2:5797655/101‑1 (MQ=255)
aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAg                                                                                               <  2:4333257/101‑1 (MQ=255)
aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAg                                                                                               >  1:4455501/1‑101 (MQ=255)
  gatctcGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCa                                                                                             <  1:6261711/96‑1 (MQ=255)
  gCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCa                                                                                             <  2:4745073/101‑1 (MQ=255)
    gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATg                                                                                           <  2:1704887/101‑1 (MQ=255)
        gTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCaa                                                                                       <  2:2359590/101‑1 (MQ=255)
         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAAc                                                                                      <  1:5794972/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                                     <  1:3271428/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                                     <  2:4485041/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                                     <  2:209576/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                                     <  2:5166022/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                                     >  1:4550411/1‑101 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                                     <  2:7001854/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGNCGCCAAGCATGGCAACt                                                                                     >  2:2427624/1‑101 (MQ=255)
            ccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTgg                                                                                   <  1:6987199/101‑1 (MQ=255)
            ccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTgg                                                                                   <  1:6217073/101‑1 (MQ=255)
              gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGct                                                                                 >  2:3348748/1‑101 (MQ=255)
                 tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTg                                                                              <  2:5301881/101‑1 (MQ=255)
                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                                             <  1:6046497/101‑1 (MQ=255)
                    ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGc                                                                           <  1:4028781/101‑1 (MQ=255)
                    ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCCCTGGGc                                                                           >  1:104303/1‑101 (MQ=255)
                     tCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGcc                                                                          <  2:1009669/101‑1 (MQ=255)
                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                         <  2:6512826/101‑1 (MQ=255)
                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                         >  1:1565687/1‑101 (MQ=255)
                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                         >  1:6725988/1‑101 (MQ=255)
                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                         >  1:842670/1‑101 (MQ=255)
                       gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                        >  1:2083172/1‑101 (MQ=255)
                        tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                                                       >  1:4210598/1‑101 (MQ=255)
                        tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                                                       <  1:3136964/101‑1 (MQ=255)
                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTCGa                                                                      >  1:519533/1‑101 (MQ=255)
                          gccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                                     <  2:1602581/100‑1 (MQ=255)
                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                   >  1:1969670/1‑101 (MQ=255)
                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                  <  2:6275568/101‑2 (MQ=255)
                                cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                               >  1:1206809/1‑97 (MQ=255)
                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                             >  1:7130434/1‑101 (MQ=255)
                                  cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                            >  1:50371/1‑101 (MQ=255)
                                  cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                            >  1:1634790/1‑101 (MQ=255)
                                  cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                            >  1:314566/1‑101 (MQ=255)
                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                           >  1:5775520/1‑100 (MQ=255)
                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                           <  1:2650603/101‑2 (MQ=255)
                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCCGGCGCCG.CACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                           >  1:7090908/1‑100 (MQ=255)
                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                          <  1:5263421/101‑3 (MQ=255)
                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                         >  2:1518723/1‑98 (MQ=255)
                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                         >  1:4876632/1‑98 (MQ=255)
                                      gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                        <  2:5413391/101‑5 (MQ=255)
                                       aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                      <  1:3015912/101‑1 (MQ=255)
                                       aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                      <  2:2930103/101‑1 (MQ=255)
                                          cGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                                                   >  1:6572661/1‑101 (MQ=255)
                                             ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                >  2:4249869/1‑101 (MQ=255)
                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                                               <  1:2420919/101‑1 (MQ=255)
                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                                               >  2:2632605/1‑101 (MQ=255)
                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                              <  2:4642489/101‑1 (MQ=255)
                                                   ccGATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                          <  2:3027148/101‑1 (MQ=255)
                                                     gATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                        >  1:6490817/1‑101 (MQ=255)
                                                     gATCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                        >  2:2514437/1‑101 (MQ=255)
                                                       tCATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                      <  1:3466824/101‑3 (MQ=255)
                                                        cATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                     <  2:3564890/101‑4 (MQ=255)
                                                        cATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                     >  1:5149752/1‑98 (MQ=255)
                                                        cATGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                     >  1:59795/1‑98 (MQ=255)
                                                         aTGTCGGCTTCGCGCTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                    <  1:1671465/101‑5 (MQ=255)
                                                           gTCGGCTTCGCGCTGGC.GCGTAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                 <  1:4379137/101‑1 (MQ=255)
                                                             cggcttcgcgcTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                               >  2:5929878/1‑101 (MQ=255)
                                                             cggcttcgcgcTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                               >  1:5435392/1‑101 (MQ=255)
                                                              ggcttcgcgcTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                              <  2:1305509/101‑1 (MQ=255)
                                                              ggcttcgcgcTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                              >  2:3464353/1‑101 (MQ=255)
                                                              ggcttcgcgcTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                              >  1:5540870/1‑101 (MQ=255)
                                                               gcttcgcgcTGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                             >  2:537048/1‑101 (MQ=255)
                                                                    gcgctGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                        <  2:5572228/99‑1 (MQ=255)
                                                                     cgctGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                       <  1:1172521/100‑1 (MQ=255)
                                                                      gctGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                      >  1:3648980/1‑101 (MQ=255)
                                                                      gctGGCGCCG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                      >  1:181262/1‑101 (MQ=255)
                                                                       ctggcgccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                     <  2:811531/95‑1 (MQ=255)
                                                                       ctggcgccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                     <  1:6876219/95‑1 (MQ=255)
                                                                       ctggcgccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                     >  1:4869887/7‑101 (MQ=255)
                                                                       ctggcgccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                     >  2:3888818/7‑101 (MQ=255)
                                                                        tggcgccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                    <  2:7130060/96‑1 (MQ=255)
                                                                        tggcgccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                    <  1:4415964/96‑1 (MQ=255)
                                                                        tggcgccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGCCAC.CGAAAAGGCCGAGTGGGGCCTGaaa                    >  1:5706828/6‑101 (MQ=255)
                                                                         ggcgccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                   >  2:3839004/5‑101 (MQ=255)
                                                                            gccG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg                >  2:1445350/2‑101 (MQ=255)
                                                                              cG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCGGAGGCGGCGCTGAAATtcgtc              >  2:2890651/1‑101 (MQ=255)
                                                                              cG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc              <  1:5894236/101‑1 (MQ=255)
                                                                              cG.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc              <  2:2732628/101‑1 (MQ=255)
                                                                               g.AACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg             <  1:1345423/101‑1 (MQ=255)
                                                                                 aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa            <  2:6021427/101‑1 (MQ=255)
                                                                                 aaCAAGGCCGGTGTCGCCAAGCATGGCAACCGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGACGCGGCGCCGAAATtcgtcgg            >  2:4403840/1‑100 (MQ=255)
                                                                                  aCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa           <  2:4161178/101‑1 (MQ=255)
                                                                                   cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt          >  2:6748004/1‑101 (MQ=255)
                                                                                   cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt          <  1:5314079/101‑1 (MQ=255)
                                                                                   cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt          <  2:5669244/101‑1 (MQ=255)
                                                                                    aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg         >  1:332387/6‑101 (MQ=255)
                                                                                     aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg        >  2:5327877/5‑101 (MQ=255)
                                                                                        ccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg     <  2:1969598/100‑1 (MQ=255)
                                                                                          ggTGTCGCCAAGCATGGCAAGTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAACGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc   >  1:6386866/1‑101 (MQ=255)
                                                                                          ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc   <  1:2258317/101‑1 (MQ=255)
                                                                                          ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc   >  1:2457003/1‑101 (MQ=255)
                                                                                          ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAATCGTCGAAGGGGCGAc   >  2:468411/1‑101 (MQ=255)
                                                                                           gtgtCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAcc  <  2:4869643/101‑1 (MQ=255)
                                                                                           gtgtCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAcc  >  2:4033092/1‑101 (MQ=255)
                                                                                                ||                                                                                                 
ATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCG.AACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACC  >  NC_014034/2180093‑2180283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG