Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,307,862 3 bp→GGC 100% coding (933‑935/1203 nt) RCAP_rcc02140 ← extracellular ligand‑binding receptor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,307,8620C→G100.0% 89.9 223G312A (GGC→GCC) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (87/102):  ref base (29/5):  total (116/107)
Fisher's exact test strand distribution p-value = 2.28e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_0140342,307,8630C→G100.0% 818.5 223G312R (GGC→CGC) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (115/107):  ref base (1/0):  total (116/107)
*NC_0140342,307,8640G→C100.0% 89.5 223N311K (AAC→AAG) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (86/102):  ref base (29/5):  total (116/107)
Fisher's exact test strand distribution p-value = 2.24e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

TAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCG  >  NC_014034/2307772‑2307952
                                                                                          |||                                                                                         
tAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCCGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTcgccgc                                                                                  >  1:366745/1‑101 (MQ=255)
 aGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGA.TTCgccgcc                                                                                 >  1:5704160/1‑101 (MQ=255)
 aGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCACCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCgccgcc                                                                                 <  1:6328434/101‑1 (MQ=255)
   cTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCgg                                                                               >  2:1417851/1‑101 (MQ=255)
     ggAACGGGTCGGGTTCGGCCCCGCGGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGAt                                                                             >  2:3401639/1‑101 (MQ=255)
      gAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATc                                                                            >  2:4240454/1‑101 (MQ=255)
      gAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATc                                                                            >  2:2560762/1‑101 (MQ=255)
      gAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCCGC.TTCGCCGCCGGATc                                                                            >  1:3297202/1‑101 (MQ=255)
       aaCGGGTCGGGTTCGCCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATcc                                                                           <  1:6100877/101‑1 (MQ=255)
        aCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCa                                                                          <  1:1005987/101‑1 (MQ=255)
         cgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAg                                                                         >  1:1494890/1‑101 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAgc                                                                        >  2:2230003/1‑101 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAgc                                                                        >  1:1030553/1‑101 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAgc                                                                        >  1:2842963/1‑101 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAgc                                                                        <  1:2810936/101‑1 (MQ=255)
             tcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgcc                                                                     >  2:1797021/1‑101 (MQ=255)
             ggggtctCGGCCCCGCCCAGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgcc                                                                     <  2:1142210/95‑1 (MQ=255)
              cgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    >  2:5761400/1‑101 (MQ=255)
              cgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    >  1:4252016/1‑101 (MQ=255)
              cgggtCCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    <  2:3640925/101‑1 (MQ=255)
               gggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  2:2954608/1‑101 (MQ=255)
               gggtTCGGCCCCACCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  1:6396500/1‑101 (MQ=255)
                 gtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGa                                                                 >  2:5715392/1‑101 (MQ=255)
                  ttCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAg                                                                >  1:721816/1‑101 (MQ=255)
                  ttCGGCCCCGCCGCGCGCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAg                                                                <  2:4183679/101‑1 (MQ=255)
                   tCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgc                                                               <  2:4412917/101‑1 (MQ=255)
                     ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             >  1:1569054/1‑101 (MQ=255)
                     ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             <  2:525693/101‑1 (MQ=255)
                     ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             >  2:4872883/1‑101 (MQ=255)
                      gCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgcc                                                            <  1:6483257/101‑1 (MQ=255)
                         ccgccgCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCgccgcc                                                         >  1:2789069/1‑101 (MQ=255)
                         ccgccgCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCgccgcc                                                         >  1:4151538/1‑101 (MQ=255)
                          cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                        <  1:5176129/101‑1 (MQ=255)
                          cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                        >  1:7106087/1‑101 (MQ=255)
                            ccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGa                                                      <  1:5888977/101‑1 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     <  2:2289932/101‑1 (MQ=255)
                              gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    >  1:3453212/1‑101 (MQ=255)
                              ccgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    <  2:1227187/100‑1 (MQ=255)
                               cgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAg                                                   >  1:3725356/1‑101 (MQ=255)
                                ggCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                  >  2:3806422/1‑101 (MQ=255)
                                  ccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgc                                                <  1:4066664/101‑1 (MQ=255)
                                   cagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgcg                                               >  2:2717215/1‑101 (MQ=255)
                                    agcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgcgc                                              <  2:1313622/101‑1 (MQ=255)
                                         cgccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacca                                         <  1:5762651/101‑1 (MQ=255)
                                          gccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCcaccac                                        <  2:500479/101‑1 (MQ=255)
                                           ccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCaccacc                                       >  1:1642985/1‑101 (MQ=255)
                                           ccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCaccacc                                       <  2:4485564/101‑1 (MQ=255)
                                            cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                      >  1:6044315/1‑101 (MQ=255)
                                             agcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                     <  2:6495669/101‑1 (MQ=255)
                                                gccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAAt                                  >  1:2108289/1‑101 (MQ=255)
                                                 ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 <  2:1537512/101‑1 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                               >  2:7054177/1‑101 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                               >  2:2662198/1‑101 (MQ=255)
                                                    gACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCg                              <  1:5113566/101‑1 (MQ=255)
                                                     aCGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCgg                             >  1:3360348/1‑101 (MQ=255)
                                                     aCGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCgg                             >  2:5867487/1‑101 (MQ=255)
                                                         ccGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTc                         >  2:655972/1‑101 (MQ=255)
                                                          cGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCg                        >  1:6585600/1‑101 (MQ=255)
                                                           ggTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGa                       >  2:5603823/1‑101 (MQ=255)
                                                           ggTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGa                       <  2:4280609/101‑1 (MQ=255)
                                                           ggTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGa                       <  1:6888012/101‑1 (MQ=255)
                                                             taaGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                     <  1:1186987/99‑1 (MQ=255)
                                                             tCAGATAGTCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCTGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCCCCGGAATGTCGGTGTCGAAc                     <  2:6191078/101‑1 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                    >  1:2581703/1‑101 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                    <  2:3725206/101‑1 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                    >  2:6007107/1‑101 (MQ=255)
                                                                 atggCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGAt                 <  2:3867787/98‑1 (MQ=255)
                                                                 aTAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGAt                 <  1:1665841/101‑1 (MQ=255)
                                                                  tAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                <  1:4820597/101‑1 (MQ=255)
                                                                  tAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                >  2:1164823/1‑101 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               <  1:2230098/101‑1 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               <  1:1312990/101‑1 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               >  1:184941/1‑101 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               <  2:5684530/101‑1 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               >  1:4400184/1‑101 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               >  1:4339791/1‑101 (MQ=255)
                                                                      ccTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGc            >  1:2533674/1‑101 (MQ=255)
                                                                       cTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCa           >  1:1253656/1‑101 (MQ=255)
                                                                        tCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          <  1:6205768/101‑1 (MQ=255)
                                                                        tCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          <  1:5641763/101‑1 (MQ=255)
                                                                        tCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          >  2:6705013/1‑101 (MQ=255)
                                                                         cGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg         <  1:3803248/101‑1 (MQ=255)
                                                                         cGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg         >  1:2348737/1‑101 (MQ=255)
                                                                          gAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa        <  2:3046579/101‑1 (MQ=255)
                                                                          gAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa        >  2:739678/1‑101 (MQ=255)
                                                                           aaGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAc       >  2:2353114/1‑101 (MQ=255)
                                                                           aaGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAc       >  2:1704362/1‑101 (MQ=255)
                                                                            aGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAcc      <  2:6972435/101‑1 (MQ=255)
                                                                            aGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAcc      <  1:3214722/101‑1 (MQ=255)
                                                                            aGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAcc      >  1:3071138/1‑101 (MQ=255)
                                                                            aGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAcc      >  1:6986320/1‑101 (MQ=255)
                                                                             ggAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     <  1:1784078/101‑1 (MQ=255)
                                                                             ggAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGGCGGTGTCGAACGGATAGGGCACGACCt     >  2:3129224/1‑101 (MQ=255)
                                                                             ggAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCCCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     <  2:3453088/101‑1 (MQ=255)
                                                                              gagaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTg    <  2:20415/101‑1 (MQ=255)
                                                                              gagaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTg    <  2:6373744/101‑1 (MQ=255)
                                                                              gagaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTg    <  2:6691599/101‑1 (MQ=255)
                                                                               agaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc   <  2:4702320/101‑1 (MQ=255)
                                                                               agaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc   >  2:1978595/1‑101 (MQ=255)
                                                                                gaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCg  <  2:5415321/101‑1 (MQ=255)
                                                                                          |||                                                                                         
TAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCG  >  NC_014034/2307772‑2307952

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG