BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,620,690	2 bp→CT	100%	coding (1050‑1051/1188 nt)	livJ →	branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,620,690	0	T→C	100.0%	1280.0	340	P350P (CCT→CCC)	livJ	branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand): new base (177/161): ref base (0/1): total (177/163)
*	NC_014034	2,620,691	0	A→T	100.0%	1339.4	340	N351Y (AAC→TAC)	livJ	branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand): new base (177/162): ref base (0/1): total (177/163)

Only 100 of 347 total aligned reads displayed.


GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785

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gAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACAt                                                                                            >  1:348959/1‑101 (MQ=255)


gAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACAt                                                                                            <  2:5616572/101‑1 (MQ=255)


 aaTATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATg                                                                                           <  1:4213302/101‑1 (MQ=255)


 aaTATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATg                                                                                           <  2:2731378/101‑1 (MQ=255)


  atatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGa                                                                                          <  1:3177399/101‑1 (MQ=255)


   tatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGaa                                                                                         >  1:4337158/1‑101 (MQ=255)


        ccTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAATGGCg                                                                                    >  1:2689861/1‑101 (MQ=255)


        ccTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCg                                                                                    >  1:6372579/1‑101 (MQ=255)


         cTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCCAACCCCTAAATGAAGGGCGa                                                                                   >  2:3302612/1‑101 (MQ=255)


          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  >  2:6653218/1‑101 (MQ=255)


           gCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATg                                                                                 >  1:669041/1‑101 (MQ=255)


            cAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGc                                                                                <  1:1302741/101‑1 (MQ=255)


             aGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCg                                                                               <  1:2415938/101‑1 (MQ=255)


             aGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCg                                                                               <  1:1023003/101‑1 (MQ=255)


              gACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGa                                                                              >  2:1666329/1‑101 (MQ=255)


              gACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGa                                                                              <  2:6722112/101‑1 (MQ=255)


               aCGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGaa                                                                             <  2:4694764/101‑1 (MQ=255)


               aCGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTCCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGATGGGCGATGCGaa                                                                             <  1:5064910/101‑1 (MQ=255)


                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         >  1:2121620/1‑101 (MQ=255)


                   tGAAAGCAGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         >  2:3169755/1‑101 (MQ=255)


                    gAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGa                                                                        >  1:6571988/1‑101 (MQ=255)


                     aaaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAg                                                                       >  2:1492884/1‑101 (MQ=255)


                      aaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGc                                                                      >  1:6186588/1‑101 (MQ=255)


                      aaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGc                                                                      >  2:6854561/1‑101 (MQ=255)


                       aGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCt                                                                     <  2:5469649/101‑1 (MQ=255)


                       aGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCt                                                                     >  2:2121240/1‑101 (MQ=255)


                        gCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTa                                                                    <  2:3211354/101‑1 (MQ=255)


                         ccGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTAt                                                                   <  1:3983550/101‑1 (MQ=255)


                          cGATCCGAAGGGCCTGACCGACATTGCCGCCTTCATGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  <  1:3378923/101‑1 (MQ=255)


                          cGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  >  2:5042799/1‑101 (MQ=255)


                          cGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  >  1:5959468/1‑101 (MQ=255)


                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                <  2:1542664/101‑1 (MQ=255)


                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                <  1:1580030/101‑1 (MQ=255)


                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                <  2:4603747/101‑1 (MQ=255)


                             tCCGAAGGGCCTGACCGACACTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCg                                                               <  2:5805731/101‑1 (MQ=255)


                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            >  1:1209683/1‑101 (MQ=255)


                                 aaGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcac                                                           >  2:696301/1‑101 (MQ=255)


                                  aGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcacc                                                          >  2:936804/1‑101 (MQ=255)


                                   gggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcacca                                                         <  2:616939/101‑1 (MQ=255)


                                    ggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcaccac                                                        <  1:967762/101‑1 (MQ=255)


                                     gCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCaccacc                                                       <  1:2095177/101‑1 (MQ=255)


                                     gCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCaccacc                                                       <  2:113010/101‑1 (MQ=255)


                                      ccTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCt                                                      <  2:111/101‑1 (MQ=255)


                                       cTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTc                                                     >  1:1417369/1‑101 (MQ=255)


                                       cTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTc                                                     <  2:4274325/101‑1 (MQ=255)


                                       cTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTc                                                     >  1:4392275/1‑101 (MQ=255)


                                        tGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCg                                                    <  1:1132352/101‑1 (MQ=255)


                                         gACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGt                                                   >  2:5353291/1‑101 (MQ=255)


                                          aCCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGtt                                                  <  2:3837982/101‑1 (MQ=255)


                                           ccGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTc                                                 <  1:2326365/101‑1 (MQ=255)


                                           ccGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTc                                                 <  2:4322051/101‑1 (MQ=255)


                                            cGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCg                                                <  1:1404102/101‑1 (MQ=255)


                                            cGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCg                                                <  2:2449462/101‑1 (MQ=255)


                                             gACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCgg                                               <  1:2965110/101‑1 (MQ=255)


                                             gACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCgg                                               <  2:625445/101‑1 (MQ=255)


                                               cATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGc                                             >  2:1360164/1‑101 (MQ=255)


                                                aTTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCa                                            >  1:1982534/1‑101 (MQ=255)


                                                aTTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCa                                            <  1:4142858/101‑1 (MQ=255)


                                                  tGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGa                                          <  2:1966616/101‑1 (MQ=255)


                                                   gccgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGaa                                         <  2:4977366/101‑1 (MQ=255)


                                                    ccgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAg                                        >  2:3015766/1‑101 (MQ=255)


                                                    ccgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAg                                        >  1:2216192/1‑101 (MQ=255)


                                                      gccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCg                                      >  1:6071664/1‑101 (MQ=255)


                                                        cTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGc                                    >  1:6785859/1‑101 (MQ=255)


                                                         ttCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCa                                   >  1:405695/1‑101 (MQ=255)


                                                          tCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCCGAAGAGGCAg                                  >  1:1711047/1‑101 (MQ=255)


                                                          tCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAg                                  >  2:4865337/1‑101 (MQ=255)


                                                          tCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAg                                  >  1:7050696/1‑101 (MQ=255)


                                                           cAAGACCGCGATCGACAGTTTCGCCGCGCCGAAGCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGc                                 >  1:5022710/1‑101 (MQ=255)


                                                           cAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGc                                 >  2:3425381/1‑101 (MQ=255)


                                                           cAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGc                                 >  1:530286/1‑101 (MQ=255)


                                                           cAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGc                                 >  2:6051499/1‑101 (MQ=255)


                                                             aGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTg                               <  1:6477617/101‑1 (MQ=255)


                                                             aGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTg                               <  1:6915963/101‑1 (MQ=255)


                                                              gACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGt                              >  1:4707205/1‑101 (MQ=255)


                                                              gACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGt                              >  2:4840681/1‑101 (MQ=255)


                                                               aCCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTc                             <  2:6626022/101‑1 (MQ=255)


                                                                ccGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCg                            <  1:1492944/101‑1 (MQ=255)


                                                                ccGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCg                            <  2:6372255/101‑1 (MQ=255)


                                                                  gcgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGt                          <  1:2753619/101‑1 (MQ=255)


                                                                   cgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTg                         >  1:2618169/1‑101 (MQ=255)


                                                                   cgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTg                         >  2:4039939/1‑101 (MQ=255)


                                                                    gATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCAGCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGc                        >  1:3634092/1‑101 (MQ=255)


                                                                    gATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGc                        >  1:3755666/1‑101 (MQ=255)


                                                                    gATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGc                        >  1:55267/1‑101 (MQ=255)


                                                                    gATCGACAGTTTCGCCGCGCAGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGc                        >  2:7060569/1‑101 (MQ=255)


                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       >  1:6888943/1‑101 (MQ=255)


                                                                       cGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGc                     >  1:372400/1‑101 (MQ=255)


                                                                        gACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGcc                    >  2:180138/1‑100 (MQ=255)


                                                                        gACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCt                    <  1:4888759/101‑1 (MQ=255)


                                                                         aCAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTc                   <  1:4490473/101‑1 (MQ=255)


                                                                           aGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgt                 <  1:1893780/101‑1 (MQ=255)


                                                                              ttCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGAACCACCTCGTTCGGGCAGAAGCGGAAGCTGTCGGGGCCGCtagcggg              >  2:1031609/1‑95 (MQ=255)


                                                                                cgccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGa            <  2:4173861/101‑1 (MQ=255)


                                                                                 gccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGCTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGaa           <  2:2243660/101‑1 (MQ=255)


                                                                                    gcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGa        <  2:5193266/101‑1 (MQ=255)


                                                                                      gccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAAt      <  1:1098343/101‑1 (MQ=255)


                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATAc    >  1:1738085/1‑101 (MQ=255)


                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATAc    <  2:3655536/101‑1 (MQ=255)


                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACaa  >  2:834153/1‑101 (MQ=255)

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GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785

Base quality scores:


ATCG

< 3 ≤


ATCG

< 25 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG