Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,622	2 bp→TC	100%	intergenic (‑76/+43)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,622	0	G→T	100.0%	49.0	17	intergenic (‑76/+44)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (15/0):  ref base (2/0):  total (17/0)
*	NC_014034	774,623	0	G→C	100.0%	56.5	16	intergenic (‑77/+43)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (16/0):  ref base (0/0):  total (16/0)

CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GGT.C..GA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCG  >  NC_014034/774595‑774705                            ||                                                                                        cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg              >  2:904313/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg              >  1:888017/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGGCGGACCGGCCCCGCC.GGTCC..GACCCTACGGTCTCCTATCCTGCGATCACTTCGCCCCCCACCCGTCGGCCACg              >  1:3432203/11‑101 (MQ=255)  gcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGt             >  1:1684666/10‑101 (MQ=255)  gcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGt             >  2:5550855/10‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGTTc           >  2:6371066/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc           >  1:4138066/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc           >  1:688960/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc           >  1:7027323/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc           >  2:3251495/8‑101 (MQ=255)     ggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTcc          >  2:2832121/7‑101 (MQ=255)      gcccggcgcCCCCCCGCCCGGGGCGGACCGGCCCTGCCGGGC.C..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCCGTCGGCCACGGTccc         >  2:109810/6‑100 (MQ=255)         cggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTa      >  1:4070306/3‑101 (MQ=255)          ggcgcCCCCCCGCCCGGGTGGGACCGGCCCTGCC.GGT.CCGGC.CCTACGGTCTCTTGTCCTGCGATCACTTCGCCCGCGACCACGCGGCCACGATCCAGTAt     >  2:6639767/2‑101 (MQ=255)          ggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAt     >  1:7134034/2‑101 (MQ=255)            cgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTc   >  1:1015695/1‑101 (MQ=255)             gcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCACTATTcc  >  1:1654780/1‑100 (MQ=255)                            ||                                                                                        CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GGT.C..GA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCG  >  NC_014034/774595‑774705 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG