STRAIN: SE8H

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breseq  version 0.22  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSE8-H_1_sequence.txt7,137,451720,882,551100.0%101.0 bases101 bases95.3%
errorsSE8-H_2_sequence.txt7,137,077720,844,777100.0%101.0 bases101 bases94.3%
total14,274,5281,441,727,328100.0%101.0 bases101 bases94.8%

Reference Sequence Information

seq idlengthfit meanfit dispersiondescription
coveragedistributionNC_0140343,738,958359.55.4Rhodobacter capsulatus SB 1003 chromosome, complete genome.
coveragedistributionNC_014035132,962169.22.9Rhodobacter capsulatus SB 1003 plasmid pRCB133, complete sequence.
total3,871,920

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

Junction Evidence

reference sequencepr(no read start)
NC_0140340.25087
NC_0140350.48503

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Polymorphism E-value cutoff2
Polymorphism frequency cutoffOFF
Polymorphism minimum coverage each strandOFF
Polymorphism homopolymer length cutoffOFF
Polymorphism bias cutoff0
Polymorphism predict indel polymorphismsOFF

Execution Times

stepstartendelapsed
Read and reference sequence file input19:17:28 20 Mar 201319:25:53 20 Mar 20138 minutes 25 seconds
Read alignment to reference genome19:25:54 20 Mar 201320:37:06 20 Mar 20131 hour 11 minutes 12 seconds
Preprocessing alignments for candidate junction identification20:37:06 20 Mar 201320:51:39 20 Mar 201314 minutes 33 seconds
Preliminary analysis of coverage distribution20:51:39 20 Mar 201321:08:59 20 Mar 201317 minutes 20 seconds
Identifying junction candidates21:08:59 20 Mar 201321:09:12 20 Mar 201313 seconds
Re-alignment to junction candidates21:09:12 20 Mar 201321:27:52 20 Mar 201318 minutes 40 seconds
Resolving alignments with junction candidates21:27:52 20 Mar 201321:49:44 20 Mar 201321 minutes 52 seconds
Creating BAM files21:49:44 20 Mar 201322:05:04 20 Mar 201315 minutes 20 seconds
Tabulating error counts22:05:04 20 Mar 201322:10:13 20 Mar 20135 minutes 9 seconds
Re-calibrating base error rates22:10:13 20 Mar 201322:10:17 20 Mar 20134 seconds
Examining read alignment evidence22:10:17 20 Mar 201323:04:04 20 Mar 201353 minutes 47 seconds
Polymorphism statistics23:04:04 20 Mar 201323:04:18 20 Mar 201314 seconds
Output23:04:18 20 Mar 201323:16:52 20 Mar 201312 minutes 34 seconds
Total 3 hours 59 minutes 23 seconds