Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,074,302 Δ1 bp 1.1% coding (1371/1377 nt) RCAP_rcc00982 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,074,3020T→. 1.1% 2.5 ‑3.0 ‑0.3364coding (1371/1377 nt)RCAP_rcc00982hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (193/167):  total (193/171)
Fisher's exact test strand distribution p-value = 4.78e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.78e-01

Only 100 of 440 total aligned reads displayed.

CGCTTGGTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCTCGACCAGGG  >  NC_014034/1074202‑1074402
                                                                                                    |                                                                                                    
cgcTTGGTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCt                                                                                                      >  1:7799360/1‑101 (MQ=255)
 gcTTGGTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCtt                                                                                                     >  1:1674413/1‑101 (MQ=255)
   ttGGTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCtttt                                                                                                   >  2:1489634/1‑101 (MQ=255)
    tGGTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCttttt                                                                                                  <  1:4548393/101‑1 (MQ=255)
     ggTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCCGCGAAGCAGACGTGCCGATGCGTCAGGCtttttt                                                                                                 >  1:2078288/1‑101 (MQ=255)
     ggTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCtttttt                                                                                                 <  1:5124349/101‑1 (MQ=255)
      gTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCttttttt                                                                                                <  1:1475435/101‑1 (MQ=255)
      gTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCttttttt                                                                                                >  1:6856881/1‑101 (MQ=255)
      gTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCttttttt                                                                                                >  1:2486407/1‑101 (MQ=255)
       tGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCtttttttt                                                                                               <  2:2732616/101‑1 (MQ=255)
        gAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCttttttttt                                                                                              >  2:1104312/1‑101 (MQ=255)
        gAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCttttttttt                                                                                              <  1:8585305/101‑1 (MQ=255)
        gAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATACGTCAGGCttttttttt                                                                                              <  2:4193012/101‑1 (MQ=255)
         aaaTGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGGGAAGCAGACGTGCCGATGCGTCAGGCGTTTTTTTTg                                                                                             >  2:3396446/1‑101 (MQ=255)
         aaaTGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTg                                                                                             >  1:8403971/1‑101 (MQ=255)
          aaTGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGa                                                                                            <  1:3480586/101‑1 (MQ=255)
           aTGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAg                                                                                           <  1:4653616/101‑1 (MQ=255)
           aTGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAg                                                                                           <  2:5677553/101‑1 (MQ=255)
            tGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGc                                                                                          <  1:2341227/101‑1 (MQ=255)
            gggCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGc                                                                                          <  2:7299150/100‑1 (MQ=255)
             ggCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGcc                                                                                         >  2:1432110/1‑101 (MQ=255)
             ggCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGcc                                                                                         <  2:8986165/101‑1 (MQ=255)
             ggCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGcc                                                                                         >  1:3665058/1‑101 (MQ=255)
               cgcTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAg                                                                                       >  1:6765722/1‑101 (MQ=255)
                gcTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAgg                                                                                      <  2:6001414/101‑1 (MQ=255)
                gcTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAgg                                                                                      <  1:5790290/101‑1 (MQ=255)
                 cccGGCCGCGGAGATGGCTTGATCGGTGATCCGCTCGCCCGGCCCGCCCGGCTCAGCGAAGCAGACGCGCCGATGCGTCAGGC.TTTTTTTTGAGCCAGGAt                                                                                    <  2:6822032/99‑1 (MQ=255)
                 cTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGa                                                                                     <  2:6666373/101‑1 (MQ=255)
                   cGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCCGCTCAGCGAAGCAGCCGGGCCGATGCGTCAGGCTTTTTTTTTGAGCCGGGATg                                                                                   >  1:8719325/1‑101 (MQ=255)
                    ggCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGc                                                                                  >  1:6207731/1‑101 (MQ=255)
                      cctcAGGGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCcg                                                                                <  1:496815/98‑1 (MQ=255)
                        gCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCcgcg                                                                              >  2:6926139/1‑101 (MQ=255)
                        gCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCcgcg                                                                              >  1:5394712/1‑101 (MQ=255)
                        gCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCcgcg                                                                              >  1:4273710/1‑101 (MQ=255)
                         cAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGa                                                                             >  2:6529843/1‑101 (MQ=255)
                         cAGAGATGGCTTGATCGGTGATCCGCTCGAACGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGCTGCGTCAGGCTTTTTTTTTTAGCAAgggtatcacga                                                                             >  2:7160976/1‑92 (MQ=255)
                          agagATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGaa                                                                            >  2:4790443/1‑101 (MQ=255)
                          agagATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGaa                                                                            >  2:8065856/1‑101 (MQ=255)
                           gagaTGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCCAAGCAGACGGGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGGCGCGAAg                                                                           >  1:5965623/1‑101 (MQ=255)
                              aTGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAgggc                                                                        >  2:3649862/1‑99 (MQ=255)
                              aTGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTc                                                                        <  1:7081473/101‑1 (MQ=255)
                              aTGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTc                                                                        >  1:8227499/1‑101 (MQ=255)
                                   ttGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATc                                                                   >  1:1963330/1‑101 (MQ=255)
                                     gATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGt                                                                 <  1:2699809/101‑1 (MQ=255)
                                      gtCGGTTATCCGCCCCACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTc                                                                <  2:2914677/100‑1 (MQ=255)
                                      aTCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTc                                                                >  1:9116979/1‑101 (MQ=255)
                                       tCGGTGATCCGCTCGCCCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCt                                                               <  2:8535872/101‑1 (MQ=255)
                                       tCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCt                                                               <  2:4703090/101‑1 (MQ=255)
                                        cGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCtg                                                              <  2:1228858/101‑1 (MQ=255)
                                         ggTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCtgt                                                             >  1:7316331/1‑101 (MQ=255)
                                         ggTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCtgt                                                             >  2:6180775/1‑101 (MQ=255)
                                         ggTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCtgt                                                             >  2:8306646/1‑101 (MQ=255)
                                          gTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCtgtg                                                            <  2:3964968/101‑1 (MQ=255)
                                          gTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCtgtg                                                            >  1:2621344/1‑101 (MQ=255)
                                           tGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGa                                                           >  1:3870880/1‑101 (MQ=255)
                                            gATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGAc                                                          >  1:422798/1‑101 (MQ=255)
                                             aTCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACg                                                         >  1:1812801/1‑101 (MQ=255)
                                               ccccTCGACCGGCCCGCCCGGCTCAAGGAAGGAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGaa                                                       <  2:8718920/98‑1 (MQ=255)
                                                cGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAg                                                      <  1:2214609/101‑1 (MQ=255)
                                                  cTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAggg                                                    <  1:28557/101‑1 (MQ=255)
                                                    cGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGAt                                                  >  2:243856/1‑101 (MQ=255)
                                                    cGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGC..TTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATcg                                                <  1:6119793/101‑1 (MQ=255)
                                                     gACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGAtg                                                 >  2:3068833/1‑100 (MQ=255)
                                                     gACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATc                                                 <  2:8821361/101‑1 (MQ=255)
                                                        cGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATcggg                                              >  1:4499933/1‑101 (MQ=255)
                                                          gcccgcccGGTTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATcgggcg                                            >  2:5919683/1‑101 (MQ=255)
                                                                 cgGCTAGGCGAGCCAGACGTGCCGATGCGTCAGGCTTTTTTTTGGAGCCGGGATGCCGCGAGGGTCGGATCGTCTGGGACGAAGGGATggggcgcggcggg                                     <  2:7651898/101‑1 (MQ=255)
                                                                   gCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATcgggcgcggcgggcg                                   <  2:5154464/101‑1 (MQ=255)
                                                                      cAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATcgggcgcggcgggcgcgg                                >  1:6631208/1‑101 (MQ=255)
                                                                        gCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATcgggcgcggcgggcgcgggt                              >  2:8582586/1‑99 (MQ=255)
                                                                        gCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGtt                              <  1:5519593/101‑1 (MQ=255)
                                                                         cGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTc                             <  2:5808959/101‑1 (MQ=255)
                                                                          gAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGAGGAAGGGATggggggcggcgggcggcgggtc                            >  1:4005917/1‑94 (MQ=255)
                                                                          gAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTcc                            >  2:7801295/1‑101 (MQ=255)
                                                                            aGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGGGCGGTTCCGa                          >  1:3595603/1‑101 (MQ=255)
                                                                            aGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGAAGAAGGGATCGGGCGCGGCGGGGGCGGGTCCGa                          >  1:4380767/1‑101 (MQ=255)
                                                                             gCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGaa                         >  1:3152974/1‑101 (MQ=255)
                                                                                gACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGt                      <  2:2092046/101‑1 (MQ=255)
                                                                                  cGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGAAGGTgc                    >  2:7538315/1‑101 (MQ=255)
                                                                                       cGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGGGCGGGTCCGAAGGTGCGCCgg               >  1:4748109/1‑101 (MQ=255)
                                                                                       cGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGGGCGGGTCCGAAGGGGCGCCgg               >  2:6470266/1‑101 (MQ=255)
                                                                                       cGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCgg               <  2:8398958/101‑1 (MQ=255)
                                                                                       cGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGAAGGGGCGCCgg               >  2:7461092/1‑101 (MQ=255)
                                                                                        gATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGAAGGGGCGCCggg              >  2:3827888/1‑101 (MQ=255)
                                                                                         aTGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGGGCGGTGCCGAAGGGGCGCCGGGc             >  1:1096841/1‑101 (MQ=255)
                                                                                         aTGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGGGCGGGTCCGAAGGGGCGCCGGGc             >  1:4905313/1‑101 (MQ=255)
                                                                                          tGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGAAGGGGCGCCGGGcc            >  1:4592686/1‑101 (MQ=255)
                                                                                           gCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCt           <  1:7488875/101‑1 (MQ=255)
                                                                                            cGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGGGCGGTTCCGAAGGGGCGCCGGGCCTc          >  2:4407958/1‑101 (MQ=255)
                                                                                            cGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCTc          <  2:6340710/101‑1 (MQ=255)
                                                                                            cGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCTc          <  2:2576979/101‑1 (MQ=255)
                                                                                            cGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGAAGGGGCGCCGGGCCTc          >  1:3007389/1‑101 (MQ=255)
                                                                                             gTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCTCg         <  2:1914289/101‑1 (MQ=255)
                                                                                             gTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGAAGGGGCGCCggggcccg         >  2:5462161/1‑96 (MQ=255)
                                                                                             gTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGAAGGGGCGCCGGGCCTCg         >  1:8082994/1‑101 (MQ=255)
                                                                                                aGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCTCGAcc      <  1:1377007/101‑1 (MQ=255)
                                                                                                  gCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCTCGACCAg    <  2:1812748/101‑1 (MQ=255)
                                                                                                  gCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGGTCCGAAGGTGGGCCGGGGCTCGACCAg    >  2:3132232/1‑101 (MQ=255)
                                                                                                    tttttttttGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCTCGACCAggg  <  2:2258994/101‑1 (MQ=255)
                                                                                                    tttttttttGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGGGCGCCGGGCCTCGACCAggg  >  1:6471957/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CGCTTGGTGAAATGGCGCTCGGCCGCAGAGATGGCTTGATCGGTGATCCGCTCGACCGGCCCGCCCGGCTCAGCGAAGCAGACGTGCCGATGCGTCAGGCTTTTTTTTTGAGCCAGGATGCCGCGAAGGTCGGATCGTCTGTGACGAAGGGATCGGGCGCGGCGGGCGCGGTTCCGAAGGTGCGCCGGGCCTCGACCAGGG  >  NC_014034/1074202‑1074402

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG