Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,105,904 Δ1 bp 1.2% intergenic (+17/‑68) RCAP_rcc01027 → / → RCAP_rcc01028 hypothetical protein/iron siderophore/cobalamin ABC transporter periplasmic iron siderophore/cobalamin‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,105,9040C→. 1.2% 3.4 ‑0.4 ‑0.0327intergenic (+17/‑68)RCAP_rcc01027/RCAP_rcc01028hypothetical protein/iron siderophore/cobalamin ABC transporter periplasmic iron siderophore/cobalamin‑binding protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (127/194):  total (130/197)
Fisher's exact test strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.53e-01

Only 100 of 377 total aligned reads displayed.

CTGCGGGCCGCCGCGCGGATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCTGGCCGCG  >  NC_014034/1105804‑1106004
                                                                                                     |                                                                                                     
ctgcGGGCCGCCACGCGGATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTGCGGTCTCCGTCTCTCCTGACCCTCGCAAggcccggc                                                                                                       >  1:6507444/1‑101 (MQ=255)
   cGGGCCGCCGCGCGGATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGcccc                                                                                                    >  2:3274690/1‑101 (MQ=255)
    gggCCGCCGCGCGGATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGccccc                                                                                                   >  2:1627566/1‑101 (MQ=255)
        cgccgcGCGGATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGgcg                                                                                               >  1:9168785/1‑101 (MQ=255)
          ccgcGCGCCCCCCCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGcc                                                                                             <  1:4395339/101‑1 (MQ=255)
           cccgcgGCTCCGCCCGCCGCCGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCt                                                                                            <  2:5072414/99‑1 (MQ=255)
             cgcgGATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTgc                                                                                          >  1:8295662/1‑101 (MQ=255)
                 gATCCGCCCGCCGCTTCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.Ctt                                                                                     >  2:6065675/1‑101 (MQ=255)
                 gATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.Ctt                                                                                     >  2:7159079/1‑101 (MQ=255)
                 gATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.Ctt                                                                                     >  1:3218144/1‑101 (MQ=255)
                  ccccccccgccGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.Cttt                                                                                    <  1:5746617/96‑1 (MQ=255)
                   cccccccgccGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTc                                                                                   <  1:424759/97‑1 (MQ=255)
                    ccgcccgcCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTcc                                                                                  >  2:5251514/1‑101 (MQ=255)
                    ccccccgccGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTcc                                                                                  <  1:5440369/98‑1 (MQ=255)
                    ccccccgccGCTGCGCCCCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTcc                                                                                  <  2:8349537/98‑1 (MQ=255)
                     ccgccgccgCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCt                                                                                 <  2:1166196/98‑1 (MQ=255)
                      cccccccGCTGCGCCTCGCCCCAGAGC.CTCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTg                                                                                <  2:4233996/100‑1 (MQ=255)
                        ccgccgCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCg                                                                              <  1:8909391/101‑1 (MQ=255)
                        ccgccgCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCg                                                                              <  1:490872/101‑1 (MQ=255)
                         cgccgcTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGACCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGa                                                                             <  1:2681146/101‑1 (MQ=255)
                           ccgcTGCCCCTCGCGCCAGGCC.CGGGCGCGCCCCTGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCGGCGAcc                                                                           <  1:3081674/101‑1 (MQ=255)
                            cgcTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCg                                                                          >  2:8441942/1‑101 (MQ=255)
                            cgcTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCg                                                                          >  2:8658815/1‑101 (MQ=255)
                             gcTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCgg                                                                         >  2:6238692/1‑101 (MQ=255)
                              cTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGgc                                                                        <  1:2446002/101‑1 (MQ=255)
                              cTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCAGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGgc                                                                        <  1:2354847/101‑1 (MQ=255)
                               tGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGgcg                                                                       >  1:6296499/1‑101 (MQ=255)
                                  gcCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGccc                                                                    <  1:1548540/101‑1 (MQ=255)
                                   ccTCGCGCCAGGGCTCGGCCGC.CCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGcccc                                                                   <  2:6555499/101‑1 (MQ=255)
                                   ccTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGcccc                                                                   <  1:4530163/101‑1 (MQ=255)
                                    cTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCg                                                                  >  1:5187262/1‑101 (MQ=255)
                                    cTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCg                                                                  >  2:3330547/1‑101 (MQ=255)
                                      cgcgCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGc                                                                <  1:1094054/101‑1 (MQ=255)
                                      cgcgCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGc                                                                >  2:6674235/1‑101 (MQ=255)
                                       gcgcCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCg                                                               <  2:1258191/101‑1 (MQ=255)
                                       gcgcCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCg                                                               >  1:2203842/1‑101 (MQ=255)
                                        cgcCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGt                                                              <  1:683388/101‑1 (MQ=255)
                                           cAGTGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTAcc                                                           <  2:7745968/101‑1 (MQ=255)
                                           cAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTAcc                                                           <  2:3395981/101‑1 (MQ=255)
                                            agagC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCg                                                          >  1:8156143/1‑101 (MQ=255)
                                             gagC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCgg                                                         <  2:4671955/101‑1 (MQ=255)
                                              agC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCggg                                                        >  1:2819341/1‑100 (MQ=255)
                                              agC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGt                                                        >  2:5567218/1‑101 (MQ=255)
                                                    cgcgcgcCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTccccc                                                   <  1:8999268/101‑1 (MQ=255)
                                                      cgcgcCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCAt                                                 <  2:8259471/101‑1 (MQ=255)
                                                       gcgcCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCAtt                                                <  1:4045439/101‑1 (MQ=255)
                                                       gcgcCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCAtt                                                <  2:249245/101‑1 (MQ=255)
                                                       gcgcCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTCTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCAtt                                                <  2:5715397/101‑1 (MQ=255)
                                                         gcCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTcc                                              >  1:6171694/1‑101 (MQ=255)
                                                          cccccGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTccc                                             <  2:6390439/101‑1 (MQ=255)
                                                          cccccGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTccc                                             <  1:202383/101‑1 (MQ=255)
                                                           ccccGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCg                                            >  1:7626644/1‑101 (MQ=255)
                                                              cGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGgag                                         >  1:5313773/1‑101 (MQ=255)
                                                                tCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCGATTCCCGGAGAc                                       <  1:6613534/101‑1 (MQ=255)
                                                                 ccGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACa                                      <  1:5018580/101‑1 (MQ=255)
                                                                 ccGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACa                                      <  1:600801/101‑1 (MQ=255)
                                                                 ccGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACa                                      >  1:5881624/1‑101 (MQ=255)
                                                                  cGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCTCATTCCCGGAGACag                                     <  1:7264818/101‑1 (MQ=255)
                                                                   ggTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACaga                                    <  1:1797725/101‑1 (MQ=255)
                                                                   ggTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACaga                                    >  1:8589748/1‑101 (MQ=255)
                                                                    gTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACagag                                   <  2:5114424/101‑1 (MQ=255)
                                                                     tctcCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAgaga                                  <  2:7316951/101‑1 (MQ=255)
                                                                      ctcGGTCTCTCCTGGCCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGAt                                 <  2:4909912/101‑1 (MQ=255)
                                                                       tcCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATg                                <  1:3519338/101‑1 (MQ=255)
                                                                        ccGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGa                               <  1:7488302/101‑1 (MQ=255)
                                                                        ccGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGa                               <  2:3868677/101‑1 (MQ=255)
                                                                          gTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGaaa                             >  2:5422776/1‑101 (MQ=255)
                                                                          gTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGaaa                             <  2:8776211/101‑1 (MQ=255)
                                                                          gTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGaaa                             <  2:2899268/101‑1 (MQ=255)
                                                                            ctctcCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACt                           >  2:8584665/1‑101 (MQ=255)
                                                                             tctcCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACtt                          >  2:69600/1‑101 (MQ=255)
                                                                              ctcCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTg                         >  1:8736201/1‑101 (MQ=255)
                                                                                ccTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGcc                       >  1:2539765/1‑101 (MQ=255)
                                                                                 cTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCct                      >  2:3645145/1‑101 (MQ=255)
                                                                                   gACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCctct                    >  2:3547979/1‑101 (MQ=255)
                                                                                   gACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCctct                    <  2:2346690/101‑1 (MQ=255)
                                                                                    gcccTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCtt                   <  1:2967025/100‑1 (MQ=255)
                                                                                    aCCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCtt                   <  2:9103756/101‑1 (MQ=255)
                                                                                      ccTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCtttt                 <  1:78303/101‑1 (MQ=255)
                                                                                       cTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTg                <  2:1879683/101‑1 (MQ=255)
                                                                                          gCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCgg             >  1:973622/1‑101 (MQ=255)
                                                                                           cagggcccggcccCCGGCGCC.GCGCCTTTCCGGCGCCCGGCCCCCCGGCGT.CCCGTTCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGtt           <  1:7130210/98‑1 (MQ=255)
                                                                                           cAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGt            >  2:502848/1‑101 (MQ=255)
                                                                                           cAAGGCCCGG.CCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGtt           <  1:1138873/101‑1 (MQ=255)
                                                                                            aaGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGtt           <  2:2159137/101‑1 (MQ=255)
                                                                                            aaGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGtt           >  2:4573559/1‑101 (MQ=255)
                                                                                            aaGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGtt           >  1:3278794/1‑101 (MQ=255)
                                                                                            aaGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGtt           <  2:5187093/101‑1 (MQ=255)
                                                                                             aGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGGGACAGAGATGGAACTTTCCTCTTTTGCTGttt          >  2:2980597/1‑100 (MQ=255)
                                                                                             aGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTc          <  2:3741674/101‑1 (MQ=255)
                                                                                              ggcccggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCt         <  2:7568828/101‑1 (MQ=255)
                                                                                              ggcccggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCt         <  2:7895628/101‑1 (MQ=255)
                                                                                              ggcccggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCt         <  1:3720312/101‑1 (MQ=255)
                                                                                              ggcccggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCt         <  2:8901623/101‑1 (MQ=255)
                                                                                               gcccggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCTg        <  1:3336871/101‑1 (MQ=255)
                                                                                                cccggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATGCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCTgg       <  1:6674495/101‑1 (MQ=255)
                                                                                                 ccggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCTGGc      >  2:1042915/1‑101 (MQ=255)
                                                                                                  cggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCTGGcc     <  1:1630670/101‑1 (MQ=255)
                                                                                                   ggcccCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCTGGCcg    >  1:1312422/1‑101 (MQ=255)
                                                                                                     cccccGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCTGGCcgcg  >  2:83160/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CTGCGGGCCGCCGCGCGGATCCGCCCGCCGCTGCGCCTCGCGCCAGAGC.CGCGCGCGCCCCCGTCCGGTCTCCGTCTCTCCTGACCCTCGCAAGGCCCGGCCCCCGGCGCCTGCG.CTTTCCTGCGACCGGCGCCCCGGCGTACCGGTCCCCCATTCCCGGAGACAGAGATGAAACTTGCCTCTTTTGCGGTTCTGGCCGCG  >  NC_014034/1105804‑1106004

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG