Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,266,436 Δ1 bp 1.8% coding (165/447 nt) phnG → phosphonate metabolism protein PhnG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,266,4360G→. 1.8% 6.8 ‑0.4 ‑0.1284coding (165/447 nt)phnGphosphonate metabolism protein PhnG
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (128/151):  total (131/153)
Fisher's exact test strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.09e-01

Only 100 of 329 total aligned reads displayed.

CCGGTCCGCGCCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCCGCGACA  >  NC_014034/1266336‑1266536
                                                                                                    |                                                                                                    
  ggTCCGCGCCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCggg                                                                                                    >  1:4061865/1‑101 (MQ=255)
   gTCCGCGCCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCgggg                                                                                                   >  1:8694318/1‑101 (MQ=255)
   gTCCGCGCCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCgggg                                                                                                   <  2:4119877/101‑1 (MQ=255)
    tCCGCGCCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCggggg                                                                                                  <  1:5295203/101‑1 (MQ=255)
     ccGCGCCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCgggggg                                                                                                 <  1:8659282/101‑1 (MQ=255)
      cgcgCCTTGCGCCATTGCTGCCCCATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCggggggg                                                                                                <  1:4232900/101‑1 (MQ=255)
       gcgcCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGAGATGCTGCGCGGGCGGATGGGGGCgggggggg                                                                                               >  2:6030779/1‑101 (MQ=255)
        cgcCGGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGgc                                                                                              <  2:1994036/101‑1 (MQ=255)
         gcCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGgcg                                                                                             <  1:2407426/101‑1 (MQ=255)
          ccTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGgcgc                                                                                            <  1:607882/101‑1 (MQ=255)
          ccTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGgcgc                                                                                            <  1:2845938/101‑1 (MQ=255)
           cTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGcc                                                                                           >  2:3596123/1‑101 (MQ=255)
            tGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGccc                                                                                          >  1:6830766/1‑101 (MQ=255)
            tGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGccc                                                                                          <  1:8423694/101‑1 (MQ=255)
               cgcAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTc                                                                                       <  1:6662625/101‑1 (MQ=255)
               cgcAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTc                                                                                       >  2:4542207/1‑101 (MQ=255)
               cgcAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTc                                                                                       <  2:7039502/101‑1 (MQ=255)
               cgcAATTGCGGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTc                                                                                       <  1:1934318/101‑1 (MQ=255)
                  ccgatctgcCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAAt                                                                                    <  2:1800659/96‑1 (MQ=255)
                  aaTTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAAt                                                                                    <  1:4807988/101‑1 (MQ=255)
                  aaTTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAAt                                                                                    >  1:1727237/1‑101 (MQ=255)
                  aaTTGCTGCCCGACCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAAt                                                                                    <  2:775159/101‑1 (MQ=255)
                   aTTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCCATc                                                                                   >  2:5878063/1‑101 (MQ=255)
                    ttGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGCTGGGGGCGGGGGGGGCGACCTTCAATCt                                                                                  >  2:9022658/1‑101 (MQ=255)
                     tgctggACGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGAGGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTg                                                                                 <  2:141695/101‑1 (MQ=255)
                     tgctgcCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTg                                                                                 <  2:4588760/101‑1 (MQ=255)
                         gcCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCg                                                                             >  1:4633137/1‑101 (MQ=255)
                           ccGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCgag                                                                           <  2:4968045/101‑1 (MQ=255)
                            cGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCgaga                                                                          <  2:2297571/101‑1 (MQ=255)
                            cGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCgaga                                                                          >  1:3776946/1‑101 (MQ=255)
                             gATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGAt                                                                         <  2:2677362/101‑1 (MQ=255)
                             gATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGAt                                                                         <  1:273451/101‑1 (MQ=255)
                              aTCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATg                                                                        >  2:9138369/1‑101 (MQ=255)
                              aTCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATg                                                                        <  1:7983692/101‑1 (MQ=255)
                              aTCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATg                                                                        >  2:6126387/1‑101 (MQ=255)
                                ccgccgccTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGTGGTGATGATGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGAc                                                                      <  1:5448909/99‑1 (MQ=255)
                                cTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGAc                                                                      <  1:3159040/101‑1 (MQ=255)
                                cTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGC.GGGGGGGCGCCCTTCAATCTGGGCGAGATGACg                                                                     >  2:6310250/1‑101 (MQ=255)
                                 tGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACg                                                                     >  1:7485071/1‑101 (MQ=255)
                                 tGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACg                                                                     <  2:5956850/101‑1 (MQ=255)
                                 tGCCGCATTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGTCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACg                                                                     <  2:7824209/101‑1 (MQ=255)
                                  gccgccTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACgg                                                                    <  1:1829934/101‑1 (MQ=255)
                                      ccTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAc                                                                <  2:2386701/101‑1 (MQ=255)
                                      ccTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAc                                                                <  2:8484053/101‑1 (MQ=255)
                                       cTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcg                                                               <  2:2996176/101‑1 (MQ=255)
                                       cTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcg                                                               <  1:1317902/101‑1 (MQ=255)
                                        tttCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgc                                                              >  1:6976422/1‑101 (MQ=255)
                                         ttCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgcg                                                             >  2:1204621/1‑101 (MQ=255)
                                            gACTGGCTTCGCTTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGGGCCCTTCAATCTGGGCGGGATGACGGGGAcgcgctg                                                          >  1:6612190/1‑101 (MQ=255)
                                            gACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgcgctg                                                          >  1:6136941/1‑101 (MQ=255)
                                            gACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgcgctg                                                          <  2:6609745/101‑1 (MQ=255)
                                            gACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgcgctg                                                          <  2:882073/101‑1 (MQ=255)
                                                ggCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgcgctgcgcg                                                      >  1:1421557/1‑101 (MQ=255)
                                                 gCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGC.GGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgcgctgcgcgct                                                    >  1:1238918/1‑101 (MQ=255)
                                                   ttCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgcgctgcgcgctg                                                   >  1:8152872/1‑101 (MQ=255)
                                                   ttCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGAcgcgctgcgcgctg                                                   <  1:4149716/101‑1 (MQ=255)
                                                      gCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCgg                                                >  1:5885558/1‑101 (MQ=255)
                                                       ccTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGc                                               <  1:5934358/101‑1 (MQ=255)
                                                        cTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCt                                              >  2:5713917/1‑101 (MQ=255)
                                                        cTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCt                                              <  1:4959337/101‑1 (MQ=255)
                                                          gCCCGGACTGGGCGCGGTGATGCTGCGCGGGGGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTgg                                            <  1:8927771/101‑1 (MQ=255)
                                                          gCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTgg                                            >  2:3688503/1‑101 (MQ=255)
                                                             cGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCg                                         <  1:5065539/101‑1 (MQ=255)
                                                              gAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGa                                        >  1:6513493/1‑101 (MQ=255)
                                                              gAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGa                                        >  2:6637143/1‑101 (MQ=255)
                                                                  tGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGc                                    >  1:920879/1‑101 (MQ=255)
                                                                  tGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGc                                    >  2:737911/1‑101 (MQ=255)
                                                                   gggCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGcc                                   <  1:7776643/101‑1 (MQ=255)
                                                                       gcgGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGt                               <  2:8253462/101‑1 (MQ=255)
                                                                       gcgGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGt                               <  2:4821902/101‑1 (MQ=255)
                                                                        cgGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTg                              <  2:7297648/101‑1 (MQ=255)
                                                                         ggTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGGgg                             >  2:6658192/1‑101 (MQ=255)
                                                                         ggTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGGgg                             >  1:2696614/1‑101 (MQ=255)
                                                                          gTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTggg                            >  2:3527974/1‑101 (MQ=255)
                                                                          gTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGGggg                            >  2:3238511/1‑101 (MQ=255)
                                                                            gATGCTGCGCGGGCGGTTGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGTGTACGGTGACGCGCTGCGCGCTGCGGCTGGCCGGTCGCCGGGTGGGGc                          >  2:5351751/1‑101 (MQ=255)
                                                                            gATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGc                          <  2:2780296/101‑1 (MQ=255)
                                                                            gATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGc                          >  1:5373270/1‑101 (MQ=255)
                                                                            gATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGc                          <  1:5729929/101‑1 (MQ=255)
                                                                              tgctgcGCGGGCGGATGGGGGCGGGGGGGGAGCCCTTCAATCTGGGCGAGATGACGGGGACGCGCTGCGCGCTGCGGCTGGCCGCTGGCCGGGGGGGGGAg                        >  1:2915726/1‑101 (MQ=255)
                                                                              tgctgcGCGGGCGGAGGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAg                        <  2:5375433/101‑1 (MQ=255)
                                                                                    gcgggcggATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTc                  >  2:673819/1‑101 (MQ=255)
                                                                                    gcgggcggATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTc                  <  2:8359043/101‑1 (MQ=255)
                                                                                     cgggcggATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGGTGGCCGGGGGGGGCAGGGTTTCg                 >  2:2916499/1‑101 (MQ=255)
                                                                                     cgggcggATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCg                 >  2:2153683/1‑101 (MQ=255)
                                                                                        gcggATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGGGGGGCAGGCTTTCGTGc              >  1:3504381/1‑101 (MQ=255)
                                                                                          ggATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAg            >  2:8041933/1‑101 (MQ=255)
                                                                                          ggATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAg            <  2:8111241/101‑1 (MQ=255)
                                                                                          ggATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAg            <  1:3257879/101‑1 (MQ=255)
                                                                                           gATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGGGCCgg           >  2:1644822/1‑101 (MQ=255)
                                                                                              gggggCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGcc        <  1:6769594/101‑1 (MQ=255)
                                                                                               ggggCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCcg       <  1:7451629/101‑1 (MQ=255)
                                                                                               ggggCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGGGGGGCAGGCTTTCGTGCAGGGCcg       >  2:6540631/1‑101 (MQ=255)
                                                                                                 ggCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCcgcg     >  2:7439036/1‑101 (MQ=255)
                                                                                                 ggCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCcgcg     <  1:7577182/101‑1 (MQ=255)
                                                                                                   cGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCCGCGAc   >  1:5821026/1‑101 (MQ=255)
                                                                                                    ggggtgggCGCCCTTCAAGCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCCGCGACa  <  2:4254666/101‑1 (MQ=255)
                                                                                                    ggggggggCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCCGCGACa  >  1:6371575/1‑101 (MQ=255)
                                                                                                    ggggggggCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCCGCGACa  <  2:3978420/101‑1 (MQ=255)
                                                                                                    ggggggggCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCCGCGACa  <  2:3154068/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CCGGTCCGCGCCTGGCGCAATTGCTGCCCGATCTGCCGCCTTTCGACTGGCTTCGCCTGCCCGAACTGGGCGCGGTGATGCTGCGCGGGCGGATGGGGGCGGGGGGGGCGCCCTTCAATCTGGGCGAGATGACGGTGACGCGCTGCGCGCTGCGGCTGGCCGATGGCCGGGTGGGGCAGGCTTTCGTGCAGGGCCGCGACA  >  NC_014034/1266336‑1266536

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG