Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,268,343 Δ1 bp 0.9% coding (1057/1074 nt) phnI → phosphonate metabolism protein PhnI

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,268,3430G→. 0.9% 2.7 ‑0.5 ‑0.0452coding (1057/1074 nt)phnIphosphonate metabolism protein PhnI
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (235/212):  total (239/213)
Fisher's exact test strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.55e-01

Only 100 of 502 total aligned reads displayed.

CAACATTCAGGCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATCCGAC  >  NC_014034/1268243‑1268443
                                                                                                    |                                                                                                    
caacaTTCAGGCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCg                                                                                                      >  1:7221545/1‑101 (MQ=255)
    aTTCAGGCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCggggg                                                                                                  >  1:1903474/1‑101 (MQ=255)
    aTTCAGGCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCggggg                                                                                                  >  1:6912478/1‑101 (MQ=255)
    aTTCAGGCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCggggg                                                                                                  >  2:732407/1‑101 (MQ=255)
       cAGGCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCTCGAGGCCGGAggggc                                                                                               <  1:8833166/101‑1 (MQ=255)
        aGGCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGggggcg                                                                                              >  2:1302712/1‑101 (MQ=255)
         ggCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGggggcgg                                                                                             <  2:1013040/101‑1 (MQ=255)
            gACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGggggcggggc                                                                                          >  1:3397493/1‑101 (MQ=255)
            gACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGggggcggggc                                                                                          <  1:3786091/101‑1 (MQ=255)
            gACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGggggcggggc                                                                                          >  1:5666302/1‑101 (MQ=255)
             aCGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCa                                                                                         <  1:2247874/101‑1 (MQ=255)
             aCGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCa                                                                                         >  1:5791343/1‑101 (MQ=255)
              cGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCaa                                                                                        >  2:8749345/1‑101 (MQ=255)
               ggggTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAAt                                                                                       <  2:7521016/101‑1 (MQ=255)
               ggggTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAAt                                                                                       <  2:8482170/101‑1 (MQ=255)
                  gTTTCTTGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGAc                                                                                    <  2:4093225/101‑1 (MQ=255)
                  gTTTCTGGAGCATATCAAGCTGCCCCATTACGTGGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGAc                                                                                    >  1:6469945/1‑101 (MQ=255)
                   tttCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGagc                                                                                   >  2:8677695/1‑99 (MQ=255)
                    ttCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCt                                                                                  >  1:1040365/1‑101 (MQ=255)
                     tCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTc                                                                                 <  2:5497576/101‑1 (MQ=255)
                      cTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCa                                                                                >  2:1133602/1‑101 (MQ=255)
                      cTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCa                                                                                >  1:787974/1‑101 (MQ=255)
                      cTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCa                                                                                >  1:4148109/1‑101 (MQ=255)
                       tgaggCATATCCATCTGCCCCATTCCGTCTATTTCCAGTCCGAACTGGAACTGATCCGGCCCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAg                                                                               <  2:4234191/97‑1 (MQ=255)
                       tGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAg                                                                               <  1:7799554/101‑1 (MQ=255)
                       tGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAg                                                                               >  2:7851087/1‑101 (MQ=255)
                        ggAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGt                                                                              <  1:871532/101‑1 (MQ=255)
                        ggAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGt                                                                              <  1:5928554/101‑1 (MQ=255)
                           gCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCtt                                                                           >  2:5728830/1‑101 (MQ=255)
                           gCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCtt                                                                           <  1:1998770/101‑1 (MQ=255)
                             atatCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGCGGCCGGGGGGGCGGCGCAATGACCTCAGTCTTCg                                                                         >  2:483502/1‑101 (MQ=255)
                             atatCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGCCTTCg                                                                         >  2:3647244/1‑101 (MQ=255)
                                tCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCt                                                                      <  2:1465044/101‑1 (MQ=255)
                                 cAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCC.GGGGGGCGGGGCAATGACCTCAGTCTTCGTCttt                                                                    >  2:6187403/1‑101 (MQ=255)
                                  aaccTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGACCCGGCGCCGGCGCCGCGGGGGCGGGGGGGCGGGGCAATGCCCTCAGTCTTCGTCttt                                                                    <  1:1158567/98‑1 (MQ=255)
                                  aaGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCttt                                                                    <  1:5269307/101‑1 (MQ=255)
                                  aaGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGCAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGCCGGGGCAATGACCTCCGTCTTCGTCttt                                                                    <  1:8087983/101‑1 (MQ=255)
                                   aGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTg                                                                   >  1:4745295/1‑101 (MQ=255)
                                   aGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTg                                                                   <  2:890851/101‑1 (MQ=255)
                                   aGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGAGGGCGGGGCAATGACCTCAGTCTTCGTCTTTg                                                                   >  2:6747928/1‑101 (MQ=255)
                                   aGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCAGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTg                                                                   >  1:4339043/1‑101 (MQ=255)
                                     cTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGcc                                                                 >  2:6534816/1‑101 (MQ=255)
                                      tGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGccc                                                                <  2:8371621/101‑1 (MQ=255)
                                      tGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGccc                                                                >  2:5315164/1‑101 (MQ=255)
                                       gCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCg                                                               <  1:3474421/101‑1 (MQ=255)
                                          ccATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCAGgcg                                                            <  1:4867247/101‑1 (MQ=255)
                                           cATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGgcgc                                                           >  1:1611383/1‑101 (MQ=255)
                                           cATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGACGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGgcgc                                                           >  2:2755619/1‑101 (MQ=255)
                                            aTTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGcgcg                                                          >  1:5526947/1‑101 (MQ=255)
                                            aTTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCACGGcgcg                                                          >  1:8965434/1‑101 (MQ=255)
                                                 gTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGGCCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCt                                                     >  1:8252962/1‑101 (MQ=255)
                                                 gTCGATTGCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCt                                                     <  2:3785569/101‑1 (MQ=255)
                                                   cGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGt                                                   >  2:6469871/1‑101 (MQ=255)
                                                     aTTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTcc                                                 <  2:7669170/101‑1 (MQ=255)
                                                       ttCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCctc                                               <  2:6974077/101‑1 (MQ=255)
                                                        tCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCctct                                              >  2:8853810/1‑101 (MQ=255)
                                                        tCCAGTCCGAACTGGAACTGAACCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCctct                                              >  1:5333801/1‑101 (MQ=255)
                                                         ccAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCtctc                                             >  1:7346523/1‑101 (MQ=255)
                                                          cAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTcc                                            <  1:1236775/101‑1 (MQ=255)
                                                          cAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTcc                                            <  1:6801299/101‑1 (MQ=255)
                                                           aGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCGGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCg                                           <  1:2318050/101‑1 (MQ=255)
                                                             tCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCggg                                         >  2:1680630/1‑101 (MQ=255)
                                                             tCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCggg                                         <  1:3990078/101‑1 (MQ=255)
                                                              ccGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGgga                                        >  1:2682825/1‑101 (MQ=255)
                                                               cGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGggag                                       <  1:1302744/101‑1 (MQ=255)
                                                                gaactggaactgTTCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGggagg                                      <  1:1787612/101‑1 (MQ=255)
                                                                gaactggaactgATCCGGCGCCTGCGCCGCGATGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGggagg                                      <  1:6267170/101‑1 (MQ=255)
                                                                gaactggaactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGggagg                                      >  1:3223793/1‑101 (MQ=255)
                                                                gaactggaactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGggagg                                      >  2:5091734/1‑101 (MQ=255)
                                                                   ctggaactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATc                                   <  2:7729316/101‑1 (MQ=255)
                                                                     ggaactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATcgc                                 <  1:1470903/101‑1 (MQ=255)
                                                                      gaactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATcgcc                                <  2:3279552/101‑1 (MQ=255)
                                                                      gaactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATcgcc                                >  2:183104/1‑101 (MQ=255)
                                                                       aactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATcgccg                               >  1:3032264/1‑101 (MQ=255)
                                                                       aactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATcgccg                               <  1:5074016/101‑1 (MQ=255)
                                                                       aactgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATcgccg                               >  2:907663/1‑101 (MQ=255)
                                                                        actgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGCGGATcgccgc                              >  1:8397256/1‑101 (MQ=255)
                                                                         ctgATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCgccgcc                             <  2:6912193/101‑1 (MQ=255)
                                                                         ctgACCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGTCCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCgccgcc                             <  2:2133857/101‑1 (MQ=255)
                                                                           gATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGccgcccg                           >  1:8529982/1‑101 (MQ=255)
                                                                               cGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCtt                       <  2:7959712/101‑1 (MQ=255)
                                                                               cGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCtt                       <  2:2272939/101‑1 (MQ=255)
                                                                                ggCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTg                      >  2:4678853/1‑101 (MQ=255)
                                                                                ggCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTg                      >  1:2582415/1‑101 (MQ=255)
                                                                                     cTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTgcg                 <  1:6682625/101‑1 (MQ=255)
                                                                                     cTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTgcg                 >  2:2593453/1‑101 (MQ=255)
                                                                                      cgcggCGCGAGGGCGGGGGGGCGGGGCAATGCCCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTgcgc                <  1:2941983/100‑1 (MQ=255)
                                                                                       gcgcCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCa               >  2:3945154/1‑101 (MQ=255)
                                                                                       gcgcCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCa               <  2:3467267/101‑1 (MQ=255)
                                                                                        cgccgcGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAg              <  2:494516/101‑1 (MQ=255)
                                                                                         gccgcGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTGGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAgg             <  2:2890484/101‑1 (MQ=255)
                                                                                             cgAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACg         <  2:1205011/101‑1 (MQ=255)
                                                                                              gAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGa        <  2:8196793/101‑1 (MQ=255)
                                                                                                ggCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATc      <  1:2170633/101‑1 (MQ=255)
                                                                                                 gCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATcc     >  1:9048737/1‑101 (MQ=255)
                                                                                                   cGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATCcgc   >  2:7134806/1‑100 (MQ=255)
                                                                                                   cGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATCcga   >  1:6081620/1‑101 (MQ=255)
                                                                                                    gggggggCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATCcgac  >  1:7579977/1‑101 (MQ=255)
                                                                                                    gggggggCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATCcgac  <  1:3619024/101‑1 (MQ=255)
                                                                                                    gggggggCGGGGCAATGACCTCAGTCTTCGTCTTTGCCAGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATCcgac  >  2:3105521/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CAACATTCAGGCGACGGGGTTTCTGGAGCATATCAAGCTGCCCCATTACGTCGATTTCCAGTCCGAACTGGAACTGATCCGGCGCCTGCGCCGCGAGGCCGGGGGGGCGGGGCAATGACCTCAGTCTTCGTCTTTGCCCGGCGCGGTTCTGTCCTCTCCGGGAGGATCGCCGCCCGCCTTGGTGCGCAGGAACGATCCGAC  >  NC_014034/1268243‑1268443

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG