Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,298 Δ1 bp 1.6% coding (1180/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2980A→. 1.6% 9.4 ‑2.1 ‑0.4425coding (1180/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/6):  ref base (204/213):  total (206/219)
Fisher's exact test strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.70e-01

Only 100 of 449 total aligned reads displayed.

CGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397198‑1397398
                                                                                                      |                                                                                                    
  tgtcgtccACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                                                                                    <  2:3462796/95‑1 (MQ=255)
  tgtCTGCCACGACGCGCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                                                                                    <  2:6688716/101‑1 (MQ=255)
  tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                                                                                    <  1:6862022/101‑1 (MQ=255)
   gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                                                   >  2:8873143/1‑101 (MQ=255)
    tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAAGt                                                                                                  >  1:8503288/1‑101 (MQ=255)
     cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                                                 <  2:47477/101‑1 (MQ=255)
       gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGc                                                                                               >  2:8382665/1‑101 (MQ=255)
       gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGc                                                                                               <  2:4054247/101‑1 (MQ=255)
          acgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                            >  2:2099319/1‑101 (MQ=255)
           cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                                           >  1:398438/1‑101 (MQ=255)
            gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccc                                                                                          >  2:5772592/1‑101 (MQ=255)
             acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                                         <  1:8370823/101‑1 (MQ=255)
              cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                        <  1:6700609/101‑1 (MQ=255)
              cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                        <  2:7253571/101‑1 (MQ=255)
              cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                        <  2:6041901/101‑1 (MQ=255)
               gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCcg                                                                                       <  1:5132671/101‑1 (MQ=255)
                 ccTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCcgcg                                                                                     >  2:5538683/1‑101 (MQ=255)
                   tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCggg                                                                                   >  1:5279962/1‑101 (MQ=255)
                   tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCggg                                                                                   >  2:922496/1‑101 (MQ=255)
                    gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                                                  >  1:9129535/1‑101 (MQ=255)
                    gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                                                  <  2:8319532/101‑1 (MQ=255)
                     cACCAGATCCGCGCCGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg                                                                                 >  1:5710963/1‑101 (MQ=255)
                     cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg                                                                                 >  2:7887387/1‑101 (MQ=255)
                        cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                                              >  1:1089665/1‑101 (MQ=255)
                         ggccctcgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTg                                                                             <  1:4265387/92‑1 (MQ=2)
                         aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGCGCGTg                                                                             >  1:3865773/1‑101 (MQ=255)
                          gATCCGCGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                            <  1:7281037/101‑1 (MQ=255)
                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                   <  1:448205/101‑1 (MQ=255)
                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                   <  1:1875654/101‑1 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                 <  1:8658890/101‑1 (MQ=255)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                                >  2:119759/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                              >  2:2573198/1‑101 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACGGCTTTa                                                             >  2:4829273/1‑101 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                             <  2:3363229/101‑1 (MQ=255)
                                         cccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                             <  2:8594817/100‑1 (MQ=255)
                                          cgccccGCGGGGGCGGGGCTCGAAACACCGC.GCACGCGCGAACGGC.TCCAGACCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                            <  1:8933484/99‑1 (MQ=37)
                                          ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                            <  2:2735265/101‑1 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                            >  2:765181/1‑101 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                            <  1:3744253/101‑1 (MQ=255)
                                            ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                                          >  1:2025695/1‑101 (MQ=255)
                                            ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                                          >  2:5066944/1‑101 (MQ=255)
                                               cgcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCt                                                       <  2:3994161/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                     <  1:302245/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                     >  2:3717127/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                     <  1:1363123/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                     >  1:480070/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                                    >  2:7061400/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                                   <  2:3865664/101‑1 (MQ=255)
                                                     ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                                 >  1:8790416/1‑101 (MQ=255)
                                                      gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                                                >  2:4332650/1‑101 (MQ=255)
                                                       cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                               >  1:2489765/1‑101 (MQ=255)
                                                       cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                               <  2:6454771/101‑1 (MQ=255)
                                                         tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                             <  2:8213065/101‑1 (MQ=255)
                                                         tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                             <  2:7416130/101‑1 (MQ=255)
                                                          ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                            <  2:6533862/101‑1 (MQ=255)
                                                          ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                            >  1:1052614/1‑101 (MQ=255)
                                                           gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                           <  1:6300770/101‑1 (MQ=255)
                                                           gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                           <  1:8143991/101‑1 (MQ=255)
                                                             tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                         >  1:1583385/1‑101 (MQ=255)
                                                              cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                                        >  2:753659/1‑101 (MQ=255)
                                                                aaaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAga                                      <  1:2801888/101‑1 (MQ=255)
                                                                 aaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                                     <  2:3219855/101‑1 (MQ=255)
                                                                 aaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                                     <  1:7492117/101‑1 (MQ=255)
                                                                    acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                                  >  1:7962735/1‑101 (MQ=255)
                                                                    acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                                  <  2:180525/101‑1 (MQ=255)
                                                                     ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac                                 <  1:3257653/101‑1 (MQ=255)
                                                                      cgc.gCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg                                <  2:5286453/101‑1 (MQ=255)
                                                                        c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                              <  1:2138189/101‑1 (MQ=255)
                                                                           cACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGc                            >  1:6118238/1‑101 (MQ=255)
                                                                             cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                          <  1:3525031/101‑1 (MQ=255)
                                                                               cgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc                        >  1:7596959/1‑101 (MQ=255)
                                                                               cgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc                        <  1:5989614/101‑1 (MQ=255)
                                                                                 cgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc                      >  2:4153652/1‑101 (MQ=255)
                                                                                 cgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc                      >  1:5062531/1‑101 (MQ=255)
                                                                                  gAACGGC.GTCAGCCCGGCGAACGTCGCTCCCCCCGCGGGAGCTTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                     <  1:4689635/101‑1 (MQ=255)
                                                                                  gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                     <  1:2339523/101‑1 (MQ=255)
                                                                                   aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                    >  2:1392573/1‑101 (MQ=255)
                                                                                    aCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgc                   <  1:988137/101‑1 (MQ=255)
                                                                                     cGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg                  <  1:7745682/101‑1 (MQ=255)
                                                                                     cGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg                  >  1:6465857/1‑101 (MQ=255)
                                                                                      ggC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg                 >  2:5928602/1‑101 (MQ=255)
                                                                                      ggC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg                 <  1:6634784/101‑1 (MQ=255)
                                                                                        c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg               >  2:1856561/1‑101 (MQ=255)
                                                                                        c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg               <  2:3063168/101‑1 (MQ=255)
                                                                                          gTCAGCCCCGCGCACGTCGCACCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg              >  1:8227222/1‑101 (MQ=255)
                                                                                          gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg              >  1:7139365/1‑101 (MQ=255)
                                                                                          gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg              >  2:5138173/1‑101 (MQ=255)
                                                                                          gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg              >  1:553459/1‑101 (MQ=255)
                                                                                               ccccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGCgg         >  1:4192263/1‑101 (MQ=255)
                                                                                                 ccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt       <  1:6922662/101‑1 (MQ=255)
                                                                                                 ccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt       <  2:2844522/101‑1 (MQ=255)
                                                                                                  cgcgAGCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:9154310/101‑1 (MQ=255)
                                                                                                  cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:5710756/101‑1 (MQ=255)
                                                                                                  cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:2026324/101‑1 (MQ=255)
                                                                                                   gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg     <  1:3376655/101‑1 (MQ=255)
                                                                                                    cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa    >  1:1777109/1‑101 (MQ=255)
                                                                                                    cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa    <  1:4945685/101‑1 (MQ=255)
                                                                                                    cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa    <  2:4954157/101‑1 (MQ=255)
                                                                                                    cgAACGGCGCTTCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa    <  2:1674781/101‑1 (MQ=255)
                                                                                                     gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   >  2:4671314/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
CGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397198‑1397398

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG