Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,555 Δ1 bp 2.5% coding (1437/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5550C→. 2.5% 27.7 ‑0.9 ‑0.2523coding (1437/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/8):  ref base (260/250):  total (265/258)
Fisher's exact test strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.35e-01

Only 100 of 570 total aligned reads displayed.

TGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1397455‑1397655
                                                                                                       |                                                                                                       
 ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                                        >  1:1500884/1‑101 (MQ=255)
 ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                                        >  1:6584254/1‑101 (MQ=255)
  gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcgc                                                                                                       >  2:1358094/1‑101 (MQ=255)
    tCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccg                                                                                                     <  1:7443737/101‑1 (MQ=255)
      gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCcg.cg                                                                                                  >  1:1885229/1‑101 (MQ=255)
        aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCt                                                                                                >  1:2724713/1‑101 (MQ=255)
         acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.g                                                                                              >  1:5670027/1‑101 (MQ=255)
          cacCGGCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.gc                                                                                             >  1:8845799/1‑100 (MQ=255)
           acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.c                                                                                           >  1:6651072/1‑101 (MQ=255)
              gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTc                                                                                        <  1:7227918/101‑1 (MQ=255)
                       acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTcccccc                                                                               >  2:5530502/1‑101 (MQ=255)
                          cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCcccg                                                                            >  1:6009572/1‑101 (MQ=255)
                           aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCgg                                                                           >  1:6357932/1‑101 (MQ=255)
                             ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCgggg                                                                         <  1:5305757/101‑1 (MQ=255)
                              cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCggggg                                                                        <  1:9134068/101‑1 (MQ=255)
                               gCCCTGCAGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                       <  2:818028/101‑1 (MQ=255)
                                    gaacaggatgTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:7626390/92‑1 (MQ=2)
                                      ccgctgcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCCCCGAACCTGTCGG..CGGC.GAACACCGATGCC.G..CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:8685515/94‑1 (MQ=2)
                                       gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCG.GGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  2:6710254/96‑1 (MQ=0)
                                       cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCCCGATCCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  1:5893626/95‑1 (MQ=2)
                                       cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  1:7944222/95‑1 (MQ=2)
                                       cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  2:147275/95‑1 (MQ=2)
                                       cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  1:8182045/95‑1 (MQ=2)
                                         accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  1:4036974/101‑1 (MQ=255)
                                          tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:7878418/4‑101 (MQ=2)
                                          ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:8420429/1‑101 (MQ=255)
                                           gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  1:2464223/99‑1 (MQ=2)
                                           cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           >  2:5747191/1‑101 (MQ=255)
                                           cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           <  1:3692384/101‑1 (MQ=255)
                                            tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGGCGCTCCCCCCGCGGGGGCGTGGGTCGGAAcgg                                                         >  1:3808527/2‑99 (MQ=0)
                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCG.GGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                          <  1:4926427/101‑2 (MQ=2)
                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                          >  2:8806686/1‑101 (MQ=255)
                                            cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                                           >  2:5649787/2‑101 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGAGCGGAAcggg                                                        >  2:7092409/1‑98 (MQ=0)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                         <  1:6876012/101‑1 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                                          >  1:8639948/1‑99 (MQ=2)
                                              gcGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                        <  2:7253832/100‑1 (MQ=255)
                                               cccTCCCCCCGCGGGGGCGTGGATCGAAACGCCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                       <  2:6619657/99‑2 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                       <  1:46087/101‑2 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACaaa                                                        >  2:6809308/1‑99 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                       >  1:7722341/1‑101 (MQ=255)
                                                gccccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                      <  1:3546170/98‑1 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                       <  1:7821318/101‑4 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                     <  1:5089037/101‑1 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                    >  2:511441/1‑97 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                    >  2:7758192/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                    <  2:49121/101‑1 (MQ=255)
                                                  cccccccGCGGGGGCGGGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                    <  2:6950784/100‑5 (MQ=0)
                                                   ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCG.GGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                  <  2:2017199/101‑10 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                    >  2:4714217/1‑95 (MQ=0)
                                                   ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                    >  2:2198131/1‑95 (MQ=0)
                                                   ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                   >  2:2786619/1‑101 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                   >  2:8063310/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGGTCGGAACaaaaaggc                                                   >  2:2630170/1‑94 (MQ=0)
                                                    cccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                                   <  2:1563926/101‑8 (MQ=0)
                                                    cccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                                   <  2:1915050/101‑8 (MQ=0)
                                                    cccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG..GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                                   <  2:5851702/101‑8 (MQ=0)
                                                    cccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGa                                                  <  2:3399870/101‑1 (MQ=255)
                                                     gcccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                                 <  1:4739566/100‑8 (MQ=0)
                                                     ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                 <  1:5440802/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                <  1:7090621/101‑9 (MQ=0)
                                                      cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCG.ATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                <  1:5588757/101‑9 (MQ=0)
                                                       ccGCGGGGGGGTGGATCGAAACAACCCGATCCTGGG.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCCGCGGATCg                                               <  1:206171/101‑1 (MQ=38)
                                                          cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                            >  2:5427979/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                           <  2:9040339/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                           >  1:2584738/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                           <  2:463290/101‑1 (MQ=255)
                                                            ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                          >  1:1675914/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                          >  2:323507/1‑101 (MQ=255)
                                                               gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                       <  1:8373157/101‑1 (MQ=255)
                                                                cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                      >  2:7433993/1‑101 (MQ=255)
                                                                 gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                     >  2:3538027/1‑101 (MQ=255)
                                                                  tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCCGCGGATCGGCGTGAcccgc                                    >  2:5071472/1‑101 (MQ=255)
                                                                  tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                    >  2:1737283/1‑101 (MQ=255)
                                                                  tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                    >  1:4424096/1‑101 (MQ=255)
                                                                   ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                   >  2:6483784/1‑101 (MQ=255)
                                                                   ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                   >  1:3471056/1‑101 (MQ=255)
                                                                    gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGGGTGGGTATAAACATACGCGGAGCGGCGTGAaccgcgc                                  >  2:4280917/1‑99 (MQ=255)
                                                                    gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                  <  1:3158621/101‑1 (MQ=255)
                                                                    gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                  >  1:5187400/1‑101 (MQ=255)
                                                                     aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                 <  1:7578625/101‑1 (MQ=255)
                                                                     aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccca                                 >  2:441465/1‑100 (MQ=255)
                                                                       cGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                               >  2:7953191/1‑101 (MQ=255)
                                                                        gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                              <  2:3822088/101‑1 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                             >  2:6055311/1‑101 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                             >  2:1103476/1‑101 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                             >  1:4310151/1‑101 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                             >  1:1037424/1‑101 (MQ=255)
                                                                          aacaccCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                            <  1:2882171/101‑1 (MQ=255)
                                                                               cccGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCggc                       >  2:1262011/1‑100 (MQ=255)
                                                                                ccGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                      <  1:119759/101‑1 (MQ=255)
                                                                                    tCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc                  >  2:3076961/1‑101 (MQ=255)
                                                                                      aTGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                >  1:938789/1‑101 (MQ=255)
                                                                                      aTGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                >  1:6237215/1‑101 (MQ=255)
                                                                                      aTGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                >  1:1345103/1‑101 (MQ=255)
                                                                                          c.c..cGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCTCTCCCCCcg            <  2:4054922/101‑1 (MQ=255)
                                                                                          c.c..cGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg            >  2:5233450/1‑101 (MQ=255)
                                                                                            c..cGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc           <  2:2355797/101‑1 (MQ=255)
                                                                                               cGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg          >  2:1012326/1‑101 (MQ=255)
                                                                                                      gcgcCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGt   <  1:225391/101‑1 (MQ=255)
                                                                                                       |                                                                                                       
TGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCG.CGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1397455‑1397655

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG