Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,698 Δ1 bp 1.4% intergenic (+2/+1896) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6980A→. 1.4% 7.1 ‑3.4 ‑0.0423intergenic (+2/+1896)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (0/6):  ref base (199/218):  total (199/224)
Fisher's exact test strand distribution p-value = 3.18e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.73e-01

Only 100 of 448 total aligned reads displayed.

CATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1397598‑1397798
                                                                                                    |                                                                                                    
cATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                                      <  2:3841996/101‑1 (MQ=255)
cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                                      >  1:5118885/1‑101 (MQ=255)
cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                                      <  2:6316424/101‑1 (MQ=255)
 aTCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAg                                                                                                     <  2:5909532/101‑1 (MQ=255)
    cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTc                                                                                                  <  2:6868880/101‑1 (MQ=255)
    cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTc                                                                                                  <  2:6006531/101‑1 (MQ=255)
     gcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCg                                                                                                 <  2:1345809/101‑1 (MQ=255)
      cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGc                                                                                                >  1:5520441/1‑101 (MQ=255)
      cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGc                                                                                                <  1:232325/101‑1 (MQ=255)
       ggATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                               <  2:8090759/101‑1 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCNGGTNTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                               >  1:4080217/1‑101 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                               <  2:7044463/101‑1 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                               <  2:4668188/101‑1 (MQ=255)
        gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTc                                                                                              <  2:8815787/101‑1 (MQ=255)
        gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTc                                                                                              <  1:7927911/101‑1 (MQ=255)
          tCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccc                                                                                            <  2:8202439/101‑1 (MQ=255)
           cGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTcccc                                                                                           <  1:4615856/101‑1 (MQ=255)
            ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                                          >  1:3383319/1‑101 (MQ=255)
             gCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTcccccc                                                                                         >  1:7750063/1‑101 (MQ=255)
              cGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcg                                                                                        >  2:9160479/1‑101 (MQ=255)
                       ccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGt                                                                               <  1:8678142/101‑1 (MQ=255)
                         ccgcccTTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTTTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTgg                                                                             <  1:4281031/101‑1 (MQ=255)
                         ccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTgg                                                                             <  1:1012344/101‑1 (MQ=255)
                              cATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                        >  1:6931668/1‑101 (MQ=255)
                               aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       >  1:1535957/1‑101 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                    >  2:1480037/1‑101 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                    <  1:3603935/101‑1 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                    >  2:7182623/1‑101 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                    <  1:5862980/101‑1 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                    <  1:5643159/101‑1 (MQ=255)
                                   gTCGCTCCGCCCGCGGGGGCGTGGATCGAAGCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACtt                                                                   <  2:7930850/101‑1 (MQ=38)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTg                                                                  <  2:2880379/101‑1 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTg                                                                  <  2:5369647/101‑1 (MQ=255)
                                    tCGCGCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTg                                                                  <  2:2370259/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                                                <  2:6049892/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                                                <  2:1824900/101‑1 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                                              <  1:1450705/101‑1 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                                              <  1:3077060/101‑1 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                                              <  2:5523999/101‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                                             >  2:6580828/1‑101 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                                           >  2:1427289/1‑101 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                                           <  1:4894087/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCt                                                       <  2:4453176/101‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                      <  2:1730682/101‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTCTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGCCGTGGATCGAAACTTGTCTGACGCGCtc                                                      <  1:6525184/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     >  2:8511128/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     >  1:7626717/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     >  1:9119606/1‑101 (MQ=255)
                                                   gggCGTTGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                   <  2:116024/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatca                                                  <  1:5719794/101‑1 (MQ=255)
                                                    ggCGGGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatca                                                  <  2:2190225/101‑1 (MQ=255)
                                                      cGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                                                >  1:1271598/1‑101 (MQ=255)
                                                       gTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCCCTCATCAgga                                               >  2:3240331/1‑99 (MQ=255)
                                                         ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                             >  1:883801/1‑101 (MQ=255)
                                                         ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                             >  2:5743897/1‑101 (MQ=255)
                                                         ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                             >  1:3801364/1‑101 (MQ=255)
                                                         ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCACCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCAGCAGCACg                                             >  1:7263648/1‑101 (MQ=255)
                                                              gAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                        >  2:1977728/1‑101 (MQ=255)
                                                               aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                       <  1:8408360/101‑1 (MQ=255)
                                                                aaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt                                      <  2:2656096/101‑1 (MQ=255)
                                                                 aCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt                                     <  1:478941/101‑1 (MQ=255)
                                                                 aCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt                                     <  1:5229776/101‑1 (MQ=255)
                                                                 aCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt                                     >  1:3266079/1‑101 (MQ=255)
                                                                  cTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc                                    >  1:2700557/1‑101 (MQ=255)
                                                                   tACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa                                   >  1:3227705/1‑101 (MQ=255)
                                                                     cAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt                                 <  1:4407659/101‑1 (MQ=255)
                                                                      agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                                >  1:6299400/1‑101 (MQ=255)
                                                                      agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                                >  1:2195026/1‑101 (MQ=255)
                                                                      agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                                <  1:7875199/101‑1 (MQ=255)
                                                                        agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                              <  1:1667382/101‑1 (MQ=255)
                                                                        agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                              >  2:3644940/1‑101 (MQ=255)
                                                                        agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                              >  2:5592839/1‑101 (MQ=255)
                                                                        agTTCCGGTCTTCTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                              >  2:1661317/1‑101 (MQ=255)
                                                                         gTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCt                             <  2:7565265/101‑1 (MQ=255)
                                                                           tCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc                           >  1:3982693/1‑101 (MQ=255)
                                                                            ccGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccc                          <  2:1271566/101‑1 (MQ=255)
                                                                             cGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                         >  1:6112779/1‑101 (MQ=255)
                                                                              ggTCTAGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGCCTGACGCGCTCATCAGCACGTTAAGATTCAGTGGCTctccc                        >  2:402324/1‑101 (MQ=37)
                                                                                tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                      <  2:7722015/101‑1 (MQ=255)
                                                                                tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                      <  1:5376437/101‑1 (MQ=255)
                                                                                tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                      <  1:6962156/101‑1 (MQ=255)
                                                                                tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                      <  2:3334747/101‑1 (MQ=255)
                                                                                  ttGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcccg                    >  2:2616344/1‑101 (MQ=255)
                                                                                    gttctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggg                  >  1:1071294/1‑101 (MQ=255)
                                                                                      tctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggggg                >  1:8472466/1‑101 (MQ=255)
                                                                                       ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGGGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               >  2:4326643/1‑101 (MQ=255)
                                                                                       ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  1:7052638/101‑1 (MQ=255)
                                                                                       ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  2:5082703/101‑1 (MQ=255)
                                                                                       ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  2:5235758/101‑1 (MQ=255)
                                                                                          ttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTg            <  1:1859587/101‑1 (MQ=255)
                                                                                          ttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTg            <  2:6950303/101‑1 (MQ=255)
                                                                                             cGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGAt         >  1:1799827/1‑101 (MQ=255)
                                                                                             cGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:8157408/101‑1 (MQ=255)
                                                                                               gTACCAGGTCGCCCCCCCCGCGGGGGCGTGGATCGAACCTTGTCGGCCGCGCTCATCGGCACGTCAAGATTCAGTCGC.CCCCCCGCGGGGGCGTGGATCGa      <  2:8358321/101‑1 (MQ=25)
                                                                                                 aaCAGGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:3064293/101‑1 (MQ=255)
                                                                                                  aCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    <  2:7365580/101‑1 (MQ=255)
                                                                                                   cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCGTCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   >  2:4122917/1‑101 (MQ=255)
                                                                                                   cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   <  1:8624123/101‑1 (MQ=255)
                                                                                                    aGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAGGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct  >  1:8289398/1‑100 (MQ=255)
                                                                                                    aGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct  >  2:4946795/1‑100 (MQ=255)
                                                                                                    |                                                                                                    
CATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1397598‑1397798

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG