Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 143,582 Δ1 bp 1.1% coding (39/738 nt) lolD1 ← lipoprotein‑releasing system ATP‑binding protein LolD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034143,5820G→. 1.1% 2.8 0.0 ‑0.0374coding (39/738 nt)lolD1lipoprotein‑releasing system ATP‑binding protein LolD
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (161/209):  total (163/211)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 426 total aligned reads displayed.

TCTGCAGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAGTTTCAT  >  NC_014034/143482‑143682
                                                                                                    |                                                                                                    
tCTGCAGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCCCCCCGTCg                                                                                                      >  2:4015736/1‑101 (MQ=255)
 cTGCAGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCgg                                                                                                     >  2:468029/1‑101 (MQ=255)
   gCAGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCgggg                                                                                                   >  2:3100873/1‑101 (MQ=255)
    cAGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCggggg                                                                                                  >  2:8283059/1‑101 (MQ=255)
     aGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCgggggg                                                                                                 <  1:9074737/101‑1 (MQ=255)
     aGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCgggggg                                                                                                 <  2:279823/101‑1 (MQ=255)
     aGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCgggggg                                                                                                 >  1:2322584/1‑101 (MQ=255)
       ccGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGggc                                                                                               <  1:8527458/101‑1 (MQ=255)
        cgcgGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGggca                                                                                              <  2:3183953/101‑1 (MQ=255)
        cgcgGGACCTGCAGCACCTCCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCCCCGCCCCGTCGGGGGggca                                                                                              <  1:433788/101‑1 (MQ=255)
           gggATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGggcaggc                                                                                           >  1:8201590/1‑101 (MQ=255)
           gggATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGggcaggc                                                                                           >  1:3286491/1‑101 (MQ=255)
            ggATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGggcaggca                                                                                          <  1:5969071/101‑1 (MQ=255)
             gATCTGCAGCAAGTGCCGCCCGCCCTCGACGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCGCGCGCTGTGTCACCGCCCCGTCGGGGGGgcaggcag                                                                                         >  1:8077159/1‑101 (MQ=255)
              aTCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGcaggcagg                                                                                        <  1:8331127/101‑1 (MQ=255)
                 tGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACg                                                                                     >  2:5035728/1‑101 (MQ=255)
                 tGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACg                                                                                     >  1:7831892/1‑101 (MQ=255)
                 tGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACg                                                                                     >  1:685009/1‑101 (MQ=255)
                   cagcaACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACggg                                                                                   >  2:8400686/1‑101 (MQ=255)
                   cagcaACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACggg                                                                                   <  2:6709719/101‑1 (MQ=255)
                    agcaACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCCGGACgggg                                                                                  >  2:3374164/1‑101 (MQ=255)
                    agcaACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACgggg                                                                                  <  2:7735020/101‑1 (MQ=255)
                     gcaACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGc                                                                                 <  1:1089544/101‑1 (MQ=255)
                     gcaACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGc                                                                                 >  1:4512071/1‑101 (MQ=255)
                      caccTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCa                                                                                <  1:2917736/98‑1 (MQ=255)
                      caACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCa                                                                                <  1:4623581/101‑1 (MQ=255)
                      caACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCa                                                                                >  1:1637411/1‑101 (MQ=255)
                      caACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCa                                                                                >  1:1397757/1‑101 (MQ=255)
                       aaCTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCaa                                                                               >  1:6319119/1‑101 (MQ=255)
                        aCTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCaaa                                                                              >  1:1993047/1‑101 (MQ=255)
                         cTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAg                                                                             <  2:4595274/101‑1 (MQ=255)
                          tGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAgg                                                                            >  2:8797630/1‑101 (MQ=255)
                            gcgcccgccCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGct                                                                          <  2:6162656/100‑1 (MQ=255)
                            ccgcccgcCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGct                                                                          <  1:4016261/101‑1 (MQ=255)
                             cgcccgccCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGctc                                                                         <  2:5704204/101‑1 (MQ=255)
                              gcccgcccGCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGctct                                                                        <  2:6035316/101‑1 (MQ=255)
                              ccccgcccTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGctct                                                                        <  1:3202273/100‑1 (MQ=255)
                               cccgcccTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTg                                                                       >  2:7193062/1‑101 (MQ=255)
                                ccgcccTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGt                                                                      <  2:2230821/101‑1 (MQ=255)
                                ccgcccTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGt                                                                      >  2:1778607/1‑101 (MQ=255)
                                    ccTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGc                                                                  <  1:6433178/101‑1 (MQ=255)
                                    ccTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGc                                                                  >  2:818372/1‑101 (MQ=255)
                                      tCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCAt                                                                <  2:2464203/101‑1 (MQ=255)
                                      tCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCAt                                                                <  2:1407370/101‑1 (MQ=255)
                                      tCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCAt                                                                <  1:8751813/101‑1 (MQ=255)
                                         ccgcCCAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGtt                                                             <  1:7349504/101‑1 (MQ=255)
                                         ccgcACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGtt                                                             <  2:7624491/101‑1 (MQ=255)
                                         ccgcACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGtt                                                             >  2:208808/1‑101 (MQ=255)
                                          cgcACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTc                                                            <  1:3478469/101‑1 (MQ=255)
                                          cgcACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTc                                                            <  1:4715647/101‑1 (MQ=255)
                                           gcACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTcg                                                           <  2:7977305/101‑1 (MQ=255)
                                             acaGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTcgcg                                                         <  2:1637364/101‑1 (MQ=255)
                                              caGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCgg                                                        >  1:6039051/1‑101 (MQ=255)
                                                 ccGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTc                                                     >  1:551934/1‑101 (MQ=255)
                                                  cGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTcc                                                    <  2:3947358/101‑1 (MQ=255)
                                                   gCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCg                                                   >  2:5225199/1‑101 (MQ=255)
                                                   gCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCg                                                   <  2:4404469/101‑1 (MQ=255)
                                                    cAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTGGCGGCTCCGc                                                  >  1:4237131/1‑101 (MQ=255)
                                                        cccTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCg                                              <  2:3867788/101‑1 (MQ=255)
                                                          cTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGaa                                            >  1:2866094/1‑101 (MQ=255)
                                                          cTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGaa                                            >  2:4835890/1‑101 (MQ=255)
                                                           tCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAAt                                           <  2:5632529/101‑1 (MQ=255)
                                                            cGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATg                                          <  1:7591609/101‑1 (MQ=255)
                                                             gATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGc                                         <  1:5667243/101‑1 (MQ=255)
                                                             gATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGc                                         >  1:6141371/1‑101 (MQ=255)
                                                             gATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGc                                         <  1:7748109/101‑1 (MQ=255)
                                                                cTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGGCGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGAt                                      >  1:2163357/1‑101 (MQ=255)
                                                                   cAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTc                                   <  2:3450528/101‑1 (MQ=255)
                                                                   cAGAACAAGCCCGCGCTGTGTCGCCCCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTc                                   <  2:1241059/101‑1 (MQ=255)
                                                                      agCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGc                                <  1:4098947/101‑1 (MQ=255)
                                                                      agCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGc                                >  1:2500272/1‑101 (MQ=255)
                                                                       gCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGcc                               >  1:6662911/1‑101 (MQ=255)
                                                                         aaGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCtt                             <  1:2624395/101‑1 (MQ=255)
                                                                         aaGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCtt                             <  2:104602/101‑1 (MQ=255)
                                                                          aGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTa                            >  1:3138472/1‑101 (MQ=255)
                                                                          aGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTa                            <  1:3752819/101‑1 (MQ=255)
                                                                          aGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTa                            >  2:6602721/1‑101 (MQ=255)
                                                                            cccGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAg                          <  2:5296903/101‑1 (MQ=255)
                                                                            cccGCGCTGTGTCACCGCCCCGTC.GGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGa                         <  1:2698420/101‑1 (MQ=255)
                                                                              cgcgCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGAc                        <  2:490624/101‑1 (MQ=255)
                                                                               gcgcTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGAcg                       >  2:1955440/1‑101 (MQ=255)
                                                                                cgcTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGAcgg                      <  2:8864419/101‑1 (MQ=255)
                                                                                 gcTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGAcggc                     <  1:5776262/101‑1 (MQ=255)
                                                                                    gtgtCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGAcggcaca                  <  2:64836/101‑4 (MQ=255)
                                                                                    gtgtCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACggcggc                  <  1:6251703/101‑1 (MQ=255)
                                                                                    gggtCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACggcggc                  <  1:127757/99‑1 (MQ=255)
                                                                                       gcACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAAt               <  1:5427553/100‑1 (MQ=255)
                                                                                        cACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATg              >  1:7187157/1‑101 (MQ=255)
                                                                                         aCCGGCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGt             <  2:2909719/101‑1 (MQ=255)
                                                                                         aCCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGt             >  2:6203568/1‑101 (MQ=255)
                                                                                           cGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTaa           <  1:5073144/101‑1 (MQ=255)
                                                                                             ccccGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACa         <  2:551924/101‑1 (MQ=255)
                                                                                              cccGTGGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAg        >  2:6849973/1‑101 (MQ=255)
                                                                                               cggTCGGGGGGGCGGGAATGCGGGGGTAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAGt       <  2:7347806/99‑1 (MQ=255)
                                                                                                cGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGCTTTCAGCCTTAAGACGGCGGCAATGTAACAGtt      <  1:4785539/101‑1 (MQ=255)
                                                                                                 gTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGTTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAGttt     <  2:6261786/101‑1 (MQ=255)
                                                                                                 gTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAGttt     >  2:6997156/1‑101 (MQ=255)
                                                                                                 gTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAGttt     >  2:8605004/1‑101 (MQ=255)
                                                                                                  tCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAGTTTc    <  1:5339283/101‑1 (MQ=255)
                                                                                                    gggggggCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAGTTTCAt  >  2:266330/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCTGCAGCCGCGGGATCTGCAGCAACTGCCGCCCGCCCTCGCCGCACAGCCGCAGCCCCTCGATCTGCAGAGCAAGCCCGCGCTGTGTCACCGCCCCGTCGGGGGGGCAGGCAGGACGGGGCAAAGGCTCTGTCGGCATGTTCGCGGCTCCGCTTCGAATGCGATTTCAGCCTTAAGACGGCGGCAATGTAACAGTTTCAT  >  NC_014034/143482‑143682

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG