Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,903 Δ1 bp 98.3% intergenic (+173/‑174) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9030G→.98.3% 2.7 ‑0.6 ‑1.9267intergenic (+173/‑174)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (99/163):  ref base (2/1):  total (101/166)
Fisher's exact test strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.26e-02

Only 100 of 331 total aligned reads displayed.

GCCTTGCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAG  >  NC_014034/1607803‑1608003
                                                                                                    |                                                                                                    
  ctggCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCggg                                                                                                    <  1:6505085/98‑1 (MQ=255)
  cTTGCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGGGGCCCcgg                                                                                                    >  1:5289735/1‑101 (MQ=255)
   ttGCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCggga                                                                                                   <  1:49064/101‑2 (MQ=255)
    tGCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGGGGCGGCCCGGGGa                                                                                                  >  2:5443232/1‑101 (MQ=255)
        ggCGCCGGCTCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCg                                                                                             <  1:964822/101‑1 (MQ=255)
        ggCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGGCCGGGAaaggg                                                                                              >  2:1527435/1‑98 (MQ=255)
        ggCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGG.CCTGGGAATGCg                                                                                             <  2:5358891/101‑1 (MQ=255)
         gcgcCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGGGGCCCGGGG.ATGCgg                                                                                            >  2:5189055/1‑101 (MQ=255)
          cgcCGGATCGGGGCTGACGCCGGCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCCGGCCGGGG.ATGCggg                                                                                           >  1:7351382/1‑101 (MQ=255)
          cgcCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCggg                                                                                           <  1:3832527/101‑1 (MQ=255)
             cGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGcag                                                                                        <  1:8872969/101‑3 (MQ=255)
              ggATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCTCTCAAGGATGGCGGCGGCCCGGGGaagggggccgg                                                                                        >  1:2728828/1‑92 (MQ=255)
              ggATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAA.GCGGGcaaa                                                                                       >  1:6591029/1‑98 (MQ=255)
              ggATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGcaga                                                                                       >  1:4450223/1‑98 (MQ=255)
              ggATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGcaga                                                                                       <  2:7074796/101‑4 (MQ=255)
              ggATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGcaga                                                                                       <  1:6038846/101‑4 (MQ=255)
               gATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGcagag                                                                                      >  1:6828029/1‑97 (MQ=255)
               gATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGcagac                                                                                      <  2:4848272/101‑5 (MQ=255)
               gATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCCGCGGCCCGGGG.ATGCGGGccgac                                                                                      >  2:6081744/1‑97 (MQ=255)
                aTCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCCGGG.ATGCGGGcagccc                                                                                     >  1:8131855/1‑96 (MQ=255)
                aTCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGAcc                                                                                    <  1:3375663/101‑1 (MQ=255)
                aTCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGAcc                                                                                    <  2:2775347/101‑1 (MQ=255)
                aTCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGAcc                                                                                    <  2:8839205/101‑1 (MQ=255)
                   ggggCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCggg                                                                                 >  1:4114686/1‑101 (MQ=255)
                     ggCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCggggc                                                                               >  1:8592837/1‑99 (MQ=255)
                      gCTGACGCCGCCGCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCg                                                                              <  1:9073742/101‑1 (MQ=255)
                      gCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCg                                                                              <  2:5766916/101‑1 (MQ=255)
                       cTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCCGGCCga                                                                             >  2:2603981/1‑100 (MQ=255)
                        tGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGCAA.GCGGG.GAGACCGGGCCggc                                                                            >  2:8455497/1‑100 (MQ=255)
                           cgccgcCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGccaaccgc                                                                         >  1:1101038/1‑95 (MQ=255)
                           cgccgcCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgaccgc                                                                         <  2:656305/101‑6 (MQ=255)
                            gccgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgaccgcc                                                                        <  1:1368594/101‑7 (MQ=255)
                              cgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGccgc                                                                     <  2:4910194/101‑1 (MQ=255)
                               gccACGCCACGATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGTGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                    <  2:6002/101‑1 (MQ=255)
                                ccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCt                                                                   <  2:218324/101‑1 (MQ=255)
                                   cGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTc                                                                <  2:1478807/101‑1 (MQ=255)
                                     cccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtc                                                              <  2:8291049/101‑2 (MQ=255)
                                       cGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAGTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccg                                                            <  2:2328586/101‑4 (MQ=255)
                                        gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGGC.GACCGCCGCCTGTCtccga                                                           >  2:1872650/1‑97 (MQ=255)
                                        gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccga                                                           <  1:1962390/101‑5 (MQ=255)
                                        gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGAGGGC.AGACCGGGCC.GACCCCCGCCCGTCtccga                                                           >  2:3970641/1‑97 (MQ=255)
                                         cATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAc                                                         <  2:6123508/101‑1 (MQ=255)
                                          aTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAcc                                                        <  1:4748968/101‑1 (MQ=255)
                                           ttGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCt                                                       <  1:5528713/101‑1 (MQ=255)
                                           ttGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGACCt                                                       >  1:7020594/1‑101 (MQ=255)
                                           ttGGGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCt                                                       <  1:7779274/101‑1 (MQ=255)
                                            tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGGGGCCCGGGG.ATGCGGG.AAGACCGGGCC.GAACGACGTCTGTCtccagcctt                                                       >  2:8624530/1‑93 (MQ=255)
                                            tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                      <  2:5266717/101‑1 (MQ=255)
                                            tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                      >  2:3511172/1‑101 (MQ=255)
                                              gggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtttt                                                    <  2:7700646/100‑1 (MQ=255)
                                               ggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTc                                                   >  1:1282923/1‑101 (MQ=255)
                                               ggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTc                                                   <  1:5108059/101‑1 (MQ=255)
                                               ggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTc                                                   <  1:2804279/101‑1 (MQ=255)
                                               ggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTc                                                   >  1:6933145/1‑101 (MQ=255)
                                               ggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACC.GGCGCGACCGCCGCCTGTC.TCCGACCTTTTc                                                   >  1:7954207/1‑101 (MQ=255)
                                                gccgccGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGCCCTTTTcc                                                  >  2:2527774/1‑101 (MQ=255)
                                                gccgccGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGACCCTTTcc                                                  >  2:8114901/1‑101 (MQ=255)
                                                  cgccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                <  1:3742807/101‑1 (MQ=255)
                                                   gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                               <  2:239223/101‑1 (MQ=255)
                                                   cccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                               <  1:8068637/100‑1 (MQ=255)
                                                    ccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGc                                              <  1:1578296/101‑1 (MQ=255)
                                                     cgcgCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCg                                             <  1:2554530/101‑1 (MQ=255)
                                                        gcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAt                                          >  1:2183301/1‑101 (MQ=255)
                                                        gcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAt                                          <  1:1853901/101‑1 (MQ=255)
                                                         ccccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACGGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATc                                         <  1:5699634/101‑1 (MQ=255)
                                                         ccccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATc                                         <  2:750612/101‑1 (MQ=255)
                                                           ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                       >  1:3139500/1‑101 (MQ=255)
                                                           ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                       >  1:4243028/1‑101 (MQ=255)
                                                            cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                      >  2:1920975/1‑101 (MQ=255)
                                                             aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                     >  2:5606119/1‑101 (MQ=255)
                                                             aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                     >  2:8708903/1‑101 (MQ=255)
                                                                 cgcgcgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                 <  2:4114567/101‑1 (MQ=255)
                                                                      cccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTg                            <  1:6096533/100‑1 (MQ=255)
                                                                       ccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGc                           <  1:2400528/101‑1 (MQ=255)
                                                                        cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                          <  1:5765119/101‑1 (MQ=255)
                                                                        cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCg                          >  2:8170151/1‑101 (MQ=255)
                                                                         ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGcaa                         >  1:345384/1‑99 (MQ=255)
                                                                         ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                         >  1:2233164/1‑101 (MQ=255)
                                                                         ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                         <  1:7423507/101‑1 (MQ=255)
                                                                         ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                         <  2:1369768/101‑1 (MQ=255)
                                                                           ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACg                       >  2:9067912/1‑101 (MQ=255)
                                                                              cccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCt                    <  2:6861040/101‑1 (MQ=255)
                                                                               ccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                   <  2:6296610/101‑2 (MQ=255)
                                                                                cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                  >  1:3639781/1‑99 (MQ=255)
                                                                                 tCAAGGATGGCGGCGGCCC.GGGAATGGGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGGCCTTTTCCCAGCGGATCCCGCGTCGGGGTGCGCCCCCtgcg                 >  1:5990438/1‑98 (MQ=255)
                                                                                 tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                 >  2:3253565/1‑98 (MQ=255)
                                                                                   aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggc              >  1:1695018/1‑101 (MQ=255)
                                                                                    aGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggcg             >  1:7526206/1‑101 (MQ=255)
                                                                                      gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc           >  2:3775764/1‑101 (MQ=255)
                                                                                      gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc           >  2:1985105/1‑101 (MQ=255)
                                                                                      gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc           >  2:848747/1‑101 (MQ=255)
                                                                                      gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc           >  1:8380570/1‑101 (MQ=255)
                                                                                       aTGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgccg          >  1:3087337/1‑101 (MQ=255)
                                                                                       aTGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgccg          >  1:8741012/1‑101 (MQ=255)
                                                                                         ggcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCgccgcc        >  2:2275943/1‑101 (MQ=255)
                                                                                          ggggcggaCC.GGGAAGGC.GGCCAGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGccc       <  2:2962468/99‑1 (MQ=255)
                                                                                          gcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGccc       <  2:3055594/101‑1 (MQ=255)
                                                                                           gcgcggcCC.GGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg      <  2:4242912/99‑1 (MQ=255)
                                                                                              cggcCC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaaa   <  2:6782373/101‑1 (MQ=255)
                                                                                               ggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg  <  1:6373272/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCCTTGCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAG  >  NC_014034/1607803‑1608003

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG