Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,927 Δ1 bp 100% intergenic (+197/‑150) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9270G→.100.0% 1098.2 255intergenic (+197/‑150)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (107/147):  ref base (0/0):  total (108/147)

Only 100 of 320 total aligned reads displayed.

GCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTT  >  NC_014034/1607825‑1608025
                                                                                                      |                                                                                                  
gCTGACGCCGCCGCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCg                                                                                                    <  1:9073742/101‑1 (MQ=255)
gCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.CGACCGGGGCg                                                                                                    >  1:6756830/1‑101 (MQ=255)
    aCGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgaccg                                                                                                <  1:2192974/101‑5 (MQ=255)
      gccgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgaccgcc                                                                                              <  2:5289551/101‑7 (MQ=255)
      gccgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgaccgcc                                                                                              <  2:8353193/101‑7 (MQ=255)
      gccgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.CGGAATACGGGC.AGACCGGGCCgaccgcc                                                                                              >  2:8677465/1‑95 (MQ=255)
        cgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGccgc                                                                                           <  1:6988177/101‑1 (MQ=255)
         gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                                          <  1:575761/101‑1 (MQ=255)
          ccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCCGGCC.GACCGCCGccc                                                                                         >  2:8251094/1‑100 (MQ=255)
           cACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTg                                                                                        <  1:6555411/101‑1 (MQ=255)
           cACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTg                                                                                        <  1:2447352/101‑1 (MQ=255)
            ccGCCCGCTTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGt                                                                                       <  1:4539197/100‑1 (MQ=255)
            ccGCCCCCATTGGGGCCGCCGCGCCCCAGACCGCGCGCCCCGCCCCCTCAGGGATGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGt                                                                                       <  1:260754/100‑1 (MQ=255)
            aCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGt                                                                                       >  1:7383606/1‑101 (MQ=255)
             ggCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGTTGGCGGCTGCCC.GGGAATGCGGG.AAGACCGGGCC.GACCGCCGCCTGTc                                                                                      <  2:1044840/100‑1 (MQ=255)
             cGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTc                                                                                      <  2:1478807/101‑1 (MQ=255)
              gCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGGC.GACCGCCGCCTggcc                                                                                     >  2:170167/1‑98 (MQ=255)
              gCCCGCATTGTGCCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCt                                                                                     <  2:8728465/101‑1 (MQ=255)
                ccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtcc                                                                                   <  1:3349232/101‑3 (MQ=255)
                 cGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAGTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccg                                                                                  <  2:2328586/101‑4 (MQ=255)
                 cGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccg                                                                                  <  1:3879308/101‑4 (MQ=255)
                 cGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccg                                                                                  <  1:607485/101‑4 (MQ=255)
                  gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccga                                                                                 >  2:4105378/1‑97 (MQ=255)
                  gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGAGGGC.AGACCGGGCC.GACCCCCGCCCGTCtccga                                                                                 >  2:3970641/1‑97 (MQ=255)
                    aTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTccccgccc                                                                               >  2:6130928/1‑94 (MQ=255)
                    aTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAcc                                                                              <  1:4748968/101‑1 (MQ=255)
                     ttGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCt                                                                             >  1:2273028/1‑101 (MQ=255)
                     ttGGGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCt                                                                             <  1:7779274/101‑1 (MQ=255)
                      tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                                            >  1:1369800/1‑101 (MQ=255)
                        tgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAACtttt                                                                          >  2:84544/1‑101 (MQ=255)
                        gggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtttt                                                                          <  2:7700646/100‑1 (MQ=255)
                         ggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTc                                                                         <  1:2804279/101‑1 (MQ=255)
                          gccgccGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTcc                                                                        >  2:1537874/1‑101 (MQ=255)
                           ccgccgCGCCCCAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                                       <  2:207118/101‑1 (MQ=255)
                           ccgcccCCCCCCGGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                                       <  1:4944352/101‑1 (MQ=255)
                            cgccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                                      <  1:3742807/101‑1 (MQ=255)
                            cgccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                                      <  2:8088794/101‑1 (MQ=255)
                             gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAccagatcggaa                                                                     >  2:1899462/1‑92 (MQ=255)
                             gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccccg                                                                     >  2:8161402/1‑99 (MQ=255)
                             gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                                     >  1:8764531/1‑101 (MQ=255)
                             gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                                     <  2:239223/101‑1 (MQ=255)
                              ccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGc                                                                    >  2:2190686/1‑101 (MQ=255)
                                    cccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGG.CCGGGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATcc                                                              <  2:4905737/101‑1 (MQ=255)
                                     ccAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                             <  1:799860/101‑1 (MQ=255)
                                     ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                             >  1:8362817/1‑101 (MQ=255)
                                       aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCCGCGGATCCcgc                                                           >  2:6825142/1‑101 (MQ=255)
                                       aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                           >  2:5606119/1‑101 (MQ=255)
                                       aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                           <  1:8677850/101‑1 (MQ=255)
                                       aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCACCGGATCCcgc                                                           >  2:1921088/1‑101 (MQ=255)
                                        gaccgtggcccccGGCCGCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                                          <  2:4288666/92‑1 (MQ=255)
                                        gAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGGCCTTTTCCCAGCGGATCCcgcg                                                          >  1:1629795/1‑101 (MQ=255)
                                          aCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGAc                                                        <  2:5442419/101‑1 (MQ=255)
                                           cgcgcgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                                       <  2:8592452/101‑1 (MQ=255)
                                           cgcccgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                                       <  2:6119227/101‑1 (MQ=255)
                                               cgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGt                                                   <  2:3483830/101‑1 (MQ=255)
                                                 ccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGc                                                 <  1:5567272/101‑1 (MQ=255)
                                                  cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                                                <  1:693215/101‑1 (MQ=255)
                                                  cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                                                <  1:1224205/101‑1 (MQ=255)
                                                   ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGcaa                                               >  1:345384/1‑99 (MQ=255)
                                                     ggCCCCTCAAGGATGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTG.CCTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACg                                             <  1:7480464/101‑1 (MQ=255)
                                                     ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACg                                             >  2:9067912/1‑101 (MQ=255)
                                                      gCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGc                                            >  1:4049450/1‑101 (MQ=255)
                                                       ccccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGCGCGACGcc                                           >  2:2752009/1‑101 (MQ=255)
                                                        cccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCt                                          <  2:3185596/101‑1 (MQ=255)
                                                         ccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                                         <  2:6296610/101‑2 (MQ=255)
                                                          cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                        <  1:6189594/101‑3 (MQ=255)
                                                          cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                        >  2:5841954/1‑99 (MQ=255)
                                                          cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                        >  1:3639781/1‑99 (MQ=255)
                                                           tCAAGGATGGCGGCGGCCC.GGGAATGGGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGGCCTTTTCCCAGCGGATCCCGCGTCGGGGTGCGCCCCCtgcg                                       >  1:5990438/1‑98 (MQ=255)
                                                           tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                                       >  1:7571259/1‑98 (MQ=255)
                                                            cAAGGATGGCGGCGGCCC.GGGAATGCGGG.GAGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCACGTCCGAGCGCGACGCCtgggg                                      >  1:5178524/1‑97 (MQ=255)
                                                             aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggc                                    >  1:1695018/1‑101 (MQ=255)
                                                                gagggcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                 <  2:2588985/98‑1 (MQ=255)
                                                                gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                 >  2:848747/1‑101 (MQ=255)
                                                                gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                 >  2:3775764/1‑101 (MQ=255)
                                                                 aTGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgccg                                >  1:3087337/1‑101 (MQ=255)
                                                                    ggggcggaCC.GGGAAGGC.GGCCAGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGccc                             <  2:2962468/99‑1 (MQ=255)
                                                                       gcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaa                          <  2:5376658/101‑1 (MQ=255)
                                                                         ggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg                        <  1:6373272/101‑1 (MQ=255)
                                                                           gcccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAgg                       >  1:8070353/5‑101 (MQ=255)
                                                                              cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAggggg                    <  2:6683192/100‑1 (MQ=255)
                                                                               gggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAgggggg                   >  1:5794843/1‑101 (MQ=255)
                                                                               gggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAActgggg                   >  1:1028292/1‑101 (MQ=255)
                                                                                ggAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGc                  <  1:1872710/101‑1 (MQ=255)
                                                                                ggAATGCGGGC.AGACCGGGGC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGAGc                  >  2:2474155/1‑101 (MQ=255)
                                                                                ggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGc                  >  2:1360952/1‑101 (MQ=255)
                                                                                 gAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCt                 <  2:4967963/101‑1 (MQ=255)
                                                                                 gAAGGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCt                 <  2:2272961/101‑1 (MQ=255)
                                                                                  aaTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAcaggggggg                >  1:2137576/1‑98 (MQ=255)
                                                                                  aaTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTg                <  2:6555046/101‑1 (MQ=255)
                                                                                   aTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgc               >  1:1970163/1‑101 (MQ=255)
                                                                                     gCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgcgc             <  2:6932860/101‑1 (MQ=255)
                                                                                     gCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgcgc             <  2:5158273/101‑1 (MQ=255)
                                                                                        gggcagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgcgcgc           >  2:4691698/5‑101 (MQ=255)
                                                                                         ggcagcccGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGcc          <  1:7181298/95‑1 (MQ=255)
                                                                                            agaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGTGgcccc       >  2:7674758/1‑101 (MQ=255)
                                                                                             gaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGcccccc      <  2:2223342/101‑1 (MQ=255)
                                                                                               ccGGGCC.GACCGCCGCCTGTC.TCCGACCTTTCCCCATCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCctc    <  1:8162774/101‑1 (MQ=255)
                                                                                                cGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGACCACctct   >  1:8410884/1‑101 (MQ=255)
                                                                                                 gggCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGCGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCtt  >  2:7421076/1‑101 (MQ=255)
                                                                                                      |                                                                                                  
GCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTT  >  NC_014034/1607825‑1608025

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG