Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,398 Δ2 bp 100% intergenic (‑63/+18) ilvH ← / ← ilvI acetolactate synthase small subunit/acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,3980A→.100.0% 241.0 57intergenic (‑63/+19)ilvH/ilvIacetolactate synthase small subunit/acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (55/2):  ref base (0/0):  total (55/2)
*NC_0140341,668,3990G→.100.0% 241.0 57intergenic (‑64/+18)ilvH/ilvIacetolactate synthase small subunit/acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (55/2):  ref base (0/0):  total (55/2)

TTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668312‑1668490
                                                                                      ||                                                                                           
tttCTTGATGTTCAGAGCGGCCATTGCCCTTTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGc                                                                              >  1:7872839/1‑101 (MQ=25)
    ttGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCtctc                                                                          >  2:7848436/1‑101 (MQ=255)
       aTGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTcaca                                                                       >  1:1375638/1‑101 (MQ=255)
            cAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAgc                                                                  >  2:3398354/1‑101 (MQ=255)
             agagCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATTTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAgcg                                                                 >  2:6711097/1‑101 (MQ=37)
             agagCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAgcg                                                                 >  1:8647022/1‑101 (MQ=255)
                  ggCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCgcggcg                                                            >  2:1384360/1‑101 (MQ=255)
                      aTTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGcgccgc                                                        >  1:6509873/1‑101 (MQ=255)
                         gCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCCGCGCGGcgccgccgc                                                     >  2:4285778/1‑101 (MQ=25)
                         gCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGcgccgccag                                                     >  2:1151870/1‑99 (MQ=37)
                              ctcCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAg                                                >  2:340455/1‑101 (MQ=255)
                              ctcCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAg                                                >  1:2364329/1‑101 (MQ=255)
                                 ccTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGcc                                             >  1:7277191/1‑101 (MQ=255)
                                  cTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCt                                            >  2:8042002/1‑101 (MQ=255)
                                            tGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGc                                  >  1:6708603/1‑101 (MQ=255)
                                             gATTTGTCTAGCCGGAAATATCCCGGGGAGGCCGGGGGGCA..GCGCCACCTGCCTGCTCTCACAGCAATGCTGAGCCGCCCCGGAGAGTCTCGGCCTCGGcc                                 >  2:660624/1‑101 (MQ=255)
                                             gATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGcc                                 >  1:4529959/1‑101 (MQ=255)
                                               ttttttctGCCGGAAAATCCCCGGGGGGGCCGGGGGGC.CCCCCCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCg                                <  1:5798465/94‑1 (MQ=255)
                                                 tATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCgaga                             >  2:3516177/1‑101 (MQ=255)
                                                 tATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCgaga                             >  2:3127434/1‑101 (MQ=255)
                                                 tATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGACCCCGGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCgaga                             >  2:7829658/1‑101 (MQ=25)
                                                 tATCATGCCGGAAATATCCCGCGGGGGCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCCCGGCATCCGCCgcca                             >  2:8861015/1‑98 (MQ=25)
                                                  aTCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGagag                            >  1:5904506/1‑101 (MQ=255)
                                                  aTCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGagag                            >  1:3516871/1‑101 (MQ=255)
                                                  aTCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGACGCCGCGGAAGGCCTCGGCATCGGCCGagag                            >  1:4992348/1‑101 (MQ=37)
                                                    cTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGggca                          >  2:2726551/1‑101 (MQ=37)
                                                    cTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagca                          >  1:6838090/1‑101 (MQ=255)
                                                       gCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAgcagca                       >  2:5023647/1‑101 (MQ=255)
                                                         cGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGGGGCGCCGCCGCGGAAGCCCTCGGCATGGGCCGAGggcgcactt                     >  2:1743892/1‑95 (MQ=25)
                                                          ggAAATATCCCGGGGGGTCCGGTGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCAttt                    <  2:5911468/101‑1 (MQ=38)
                                                            aaaTATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCg                  >  1:8523693/1‑101 (MQ=255)
                                                            aaaTATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCg                  >  2:481212/1‑101 (MQ=255)
                                                             aaTATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGt                 >  1:7956944/1‑101 (MQ=255)
                                                             aaTATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGt                 >  1:4160145/1‑101 (MQ=255)
                                                              atatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGGCTGGGCACCGGCCGTGAGCAGCATTTCggt                >  2:5713535/1‑99 (MQ=25)
                                                              atatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGtt                >  1:888695/1‑101 (MQ=255)
                                                               tatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTa               >  1:8550312/1‑101 (MQ=255)
                                                                atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTAt              >  2:1911405/1‑101 (MQ=255)
                                                                atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTAt              >  1:6122646/1‑101 (MQ=255)
                                                                   cgggggggCCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCg           <  2:4200794/99‑1 (MQ=38)
                                                                   ccGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCg           >  1:9174171/1‑101 (MQ=255)
                                                                    cGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCga          >  1:4200912/1‑100 (MQ=38)
                                                                     ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc         >  2:2612302/1‑101 (MQ=255)
                                                                     ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc         >  1:571988/1‑101 (MQ=255)
                                                                     ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc         >  2:6474301/1‑101 (MQ=255)
                                                                      gggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCt        >  2:8856061/1‑101 (MQ=255)
                                                                      gggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCt        >  1:8225996/1‑101 (MQ=255)
                                                                       ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCCGCATTTCGTTATGCGGCtt       >  1:2221597/1‑101 (MQ=38)
                                                                       ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCtt       >  1:4337127/1‑101 (MQ=255)
                                                                       ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCtt       >  2:408231/1‑101 (MQ=255)
                                                                       ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCtt       >  1:3511721/1‑101 (MQ=255)
                                                                        gggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg      >  1:2201054/1‑101 (MQ=255)
                                                                        gggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg      >  2:2728435/1‑101 (MQ=255)
                                                                        gggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg      >  2:220851/1‑101 (MQ=255)
                                                                          gTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGcc    >  1:1060435/1‑101 (MQ=255)
                                                                          gTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGcc    >  2:2826091/1‑101 (MQ=255)
                                                                            ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  2:6189951/1‑101 (MQ=255)
                                                                            ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  2:5941272/1‑101 (MQ=255)
                                                                            ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  2:4644742/1‑101 (MQ=255)
                                                                            ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  2:3779474/1‑101 (MQ=255)
                                                                            ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  1:7554584/1‑101 (MQ=255)
                                                                            ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  1:7911989/1‑101 (MQ=255)
                                                                                      ||                                                                                           
TTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668312‑1668490

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 40 ≤  ATCG