Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,214 Δ1 bp 100% intergenic (‑142/+83) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,2140C→.100.0% 27.4 294intergenic (‑142/+83)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (142/140):  ref base (0/12):  total (142/152)
Fisher's exact test strand distribution p-value = 4.19e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.20e-08

Only 100 of 371 total aligned reads displayed.

ATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCTTG  >  NC_014034/1696111‑1696312
                                                                                                         |                                                                                                  
aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAac                                                                                                    >  2:5665252/1‑101 (MQ=255)
 tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                                   <  1:1253615/101‑2 (MQ=255)
 tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                                   <  2:3823933/101‑2 (MQ=255)
   tccaaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                                 <  1:902012/98‑4 (MQ=12)
   tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                                 >  2:4599292/1‑98 (MQ=255)
    cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                                                <  1:6077631/101‑5 (MQ=255)
    cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                                                <  1:8306843/101‑5 (MQ=255)
     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGTGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                               <  1:6253168/101‑6 (MQ=255)
     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                               >  2:6108718/1‑96 (MQ=12)
     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                               <  1:2775950/101‑6 (MQ=255)
      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                              <  2:4136591/101‑7 (MQ=12)
      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                              >  2:4530707/1‑95 (MQ=255)
      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                              >  2:5108435/1‑95 (MQ=255)
      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttc                                                                                              >  2:8825813/1‑95 (MQ=255)
       aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                                             <  2:2846577/101‑8 (MQ=255)
       aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                                             >  2:5466896/1‑94 (MQ=255)
       aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                                             >  1:3965131/1‑94 (MQ=255)
        tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                                            >  2:1531942/1‑93 (MQ=12)
        tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                                            >  1:5214767/1‑93 (MQ=12)
        tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                                            <  1:4390118/101‑9 (MQ=255)
         ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                                           >  1:2641450/1‑92 (MQ=255)
         ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                                           >  2:8121154/1‑92 (MQ=255)
          cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTTGGCCAA..GGTGAAacaccttgcttt                                                                                          >  2:4543699/1‑91 (MQ=255)
          cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                                          <  2:801511/101‑11 (MQ=255)
             gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                                     <  1:3639127/101‑1 (MQ=255)
             gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                                     >  2:2331953/1‑101 (MQ=255)
              cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                                    <  1:1745840/101‑1 (MQ=255)
               aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                                   >  1:8520279/1‑101 (MQ=255)
               aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                                   <  2:6785283/101‑1 (MQ=9)
                 gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                                                 <  2:1375216/101‑1 (MQ=255)
                  aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCGC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                                <  1:927419/101‑1 (MQ=9)
                  aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                                <  2:2022612/101‑1 (MQ=9)
                    ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                                              >  1:7044335/1‑101 (MQ=9)
                     tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                                             >  2:8637723/1‑101 (MQ=255)
                      ttggagggaggGGAGAGGGGC..CCTGCCCCTCCTGGCGTTTCGGCCAATTCGC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCaa                                                                            <  2:4921194/95‑1 (MQ=255)
                      ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                                            <  2:5999497/101‑1 (MQ=255)
                       tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                           >  2:3133849/1‑101 (MQ=9)
                       tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                           <  2:5303785/101‑1 (MQ=255)
                       tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                                           <  2:92221/101‑1 (MQ=255)
                        gCAAGGATGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                                          <  1:5827168/101‑1 (MQ=9)
                        gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                                          >  1:8931985/1‑101 (MQ=255)
                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCCT.GCTTTTTCTTGGCGCAAATa                                                                         >  2:7659803/1‑101 (MQ=255)
                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                                         <  2:474087/101‑1 (MQ=255)
                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                                         <  2:1763280/101‑1 (MQ=255)
                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                                         <  1:3445416/101‑1 (MQ=255)
                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                                        >  1:2114804/1‑101 (MQ=9)
                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                                        <  2:3255654/101‑1 (MQ=255)
                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                                        <  1:1327004/101‑1 (MQ=255)
                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                                        >  1:7952831/1‑101 (MQ=9)
                           aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                                       <  2:8166157/101‑1 (MQ=9)
                           aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                                       >  2:2688379/1‑101 (MQ=255)
                           aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                                       <  2:2525181/101‑1 (MQ=9)
                           aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                                       >  1:4444059/1‑101 (MQ=255)
                           aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                                       >  1:8346387/1‑101 (MQ=255)
                           aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                                       >  1:2963891/1‑101 (MQ=255)
                           aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                                       >  1:97208/1‑101 (MQ=255)
                             gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                                     >  1:1959660/1‑101 (MQ=9)
                              aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                                    >  2:7878682/1‑101 (MQ=12)
                              aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                                    <  1:5721986/101‑1 (MQ=9)
                              aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                                    <  1:1576521/101‑1 (MQ=9)
                               ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                                                   >  1:7512077/1‑101 (MQ=12)
                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                                  <  1:914930/101‑1 (MQ=255)
                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                                  >  1:4540461/1‑101 (MQ=9)
                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCatct                                                                  <  1:5665459/101‑1 (MQ=255)
                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGAGGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                                  >  1:4252860/1‑101 (MQ=9)
                                       aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                             >  2:2219663/9‑101 (MQ=12)
                                       aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                             <  1:724678/93‑1 (MQ=255)
                                         gggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                                                           >  2:3527922/7‑101 (MQ=16)
                                                  cccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                                                  <  1:7723289/101‑1 (MQ=16)
                                                  cccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                                                  >  1:7420814/1‑101 (MQ=255)
                                                          gCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa                                          >  1:4658545/1‑101 (MQ=255)
                                                             tttCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                                       >  2:536109/1‑101 (MQ=255)
                                                                cGG.CCAATCCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                                    <  2:9014228/101‑1 (MQ=19)
                                                                 ggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                                   >  1:2186895/4‑101 (MQ=255)
                                                                   ccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGc                                 >  1:4976106/2‑101 (MQ=255)
                                                                    cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc                                >  2:5190560/1‑101 (MQ=255)
                                                                    cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTCGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc                                <  1:4759114/101‑1 (MQ=25)
                                                                     aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc                               >  2:4717663/1‑101 (MQ=255)
                                                                     aaTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc                               >  1:616351/1‑101 (MQ=255)
                                                                       ttCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGATGGCGCGGccccc                             <  1:8121503/101‑1 (MQ=25)
                                                                       attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                              >  2:6539797/7‑101 (MQ=255)
                                                                       attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                              >  1:6960788/7‑101 (MQ=255)
                                                                        ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc                             >  2:1841793/6‑101 (MQ=255)
                                                                        ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc                             >  2:7397085/6‑101 (MQ=255)
                                                                          caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt                           >  1:2809217/4‑101 (MQ=255)
                                                                           cccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg                          <  1:38051/99‑1 (MQ=37)
                                                                            ccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGa                         <  2:1661294/100‑1 (MQ=37)
                                                                            ccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGa                         <  1:2520662/100‑1 (MQ=37)
                                                                             cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt                        <  2:367485/101‑1 (MQ=37)
                                                                             cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt                        >  1:6724231/1‑101 (MQ=255)
                                                                                gggATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGt                     >  1:3339434/1‑101 (MQ=255)
                                                                                 ggatgtttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTg                    <  2:4434574/95‑1 (MQ=37)
                                                                                  gatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGc                   >  2:8766413/6‑101 (MQ=255)
                                                                                     gtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc                >  2:7106235/3‑101 (MQ=255)
                                                                                     gtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc                >  2:2445747/3‑101 (MQ=255)
                                                                                      tttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa               <  2:7503655/100‑1 (MQ=38)
                                                                                        tttgggccaGGGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATGCGCCTCGGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa               <  2:3411074/93‑1 (MQ=255)
                                                                                          tgggccaaggTGAAAC..CCCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGt             <  1:5395665/93‑1 (MQ=255)
                                                                                            gcccaaggTGAAAC..CCCTTGCTTTTTCTTGGCACAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCg           <  2:7013824/95‑1 (MQ=255)
                                                                                              ccaaggTGAAAC..CCCTTGCTTTTTCTGGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAg         <  1:1001354/97‑1 (MQ=255)
                                                                                                         |                                                                                                  
ATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCTTG  >  NC_014034/1696111‑1696312

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG