BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,786,540	Δ1 bp	100%	coding (674/819 nt)	RCAP_rcc01648 →	ABC transporter permease

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,786,540	0	C→.	100.0%	69.9	388	coding (674/819 nt)	RCAP_rcc01648	ABC transporter permease
Reads supporting (aligned to +/- strand): new base (181/183): ref base (10/14): total (191/197)
Fisher's exact test strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.88e-06

Only 100 of 401 total aligned reads displayed.


AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GGCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636


   tGATGCCGCATCTTATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGGGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               <  2:4277968/101‑1 (MQ=255)


   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               <  2:3151918/101‑1 (MQ=255)


   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               >  2:150163/1‑101 (MQ=255)


   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               <  2:7386214/101‑1 (MQ=255)


   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               >  1:8748993/1‑101 (MQ=255)


    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTttc                                                                                              >  2:272898/1‑100 (MQ=255)


    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTttc                                                                                              <  1:2389258/101‑2 (MQ=255)


     aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCg                                                                                            <  2:6378520/101‑1 (MQ=255)


      tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  2:2386959/101‑1 (MQ=255)


      tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  1:3112714/101‑1 (MQ=255)


        ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                         >  1:751465/1‑101 (MQ=255)


           cATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCa                                                                                      <  2:8585969/101‑1 (MQ=255)


           cATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCa                                                                                      >  1:7817398/1‑101 (MQ=255)


            aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                     >  2:5004833/1‑101 (MQ=255)


             tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    <  2:6562969/101‑1 (MQ=255)


             tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    <  1:5624760/101‑1 (MQ=255)


             tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCCAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    <  2:3320499/101‑1 (MQ=255)


              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGATCGGGATCGG.CTTTCGTATCCATCt                                                                                   <  2:4378173/101‑1 (MQ=255)


              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                   <  2:7894668/101‑1 (MQ=255)


                  tCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAt                                                                               <  2:6095105/101‑1 (MQ=255)


                  tCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAt                                                                               <  2:1465426/101‑1 (MQ=255)


                     ccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTc                                                                            <  1:8978782/101‑1 (MQ=255)


                      cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                           >  2:3965368/1‑101 (MQ=255)


                      cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                           <  2:2232016/101‑1 (MQ=255)


                         ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                        >  2:1658968/1‑101 (MQ=255)


                             cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                    <  2:4704374/101‑1 (MQ=255)


                             cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGGCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                    >  1:930959/1‑101 (MQ=255)


                              gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   <  1:4476493/101‑1 (MQ=255)


                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  1:6342568/101‑1 (MQ=255)


                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  >  1:6731397/1‑101 (MQ=255)


                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  2:1998894/101‑1 (MQ=255)


                                 cggggctttCGCTGATCTGGTAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTGGAc                                                                <  1:7201353/95‑1 (MQ=255)


                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                >  1:4839660/7‑101 (MQ=255)


                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  2:5084644/95‑1 (MQ=255)


                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  1:462198/95‑1 (MQ=255)


                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                >  2:1599698/7‑101 (MQ=255)


                                  ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGGTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                               <  2:5624502/96‑1 (MQ=255)


                                  ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGCATCCATCTGAATTTCCAGATGTTCGACa                                                               <  1:6517758/96‑1 (MQ=255)


                                   gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                              <  2:6250531/97‑1 (MQ=255)


                                   gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                              <  2:6957144/97‑1 (MQ=255)


                                    ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             <  2:2516659/98‑1 (MQ=255)


                                    ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             <  1:4374057/98‑1 (MQ=255)


                                     gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                            <  2:8695262/99‑1 (MQ=255)


                                      ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                           <  1:7399208/100‑1 (MQ=255)


                                      ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                           <  2:7735424/100‑1 (MQ=255)


                                        ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                         <  1:8306334/101‑1 (MQ=255)


                                          cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                       >  2:5212500/1‑101 (MQ=255)


                                           gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                      <  1:137394/101‑1 (MQ=255)


                                               aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                  >  1:4712833/1‑101 (MQ=255)


                                                 cTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                >  1:5534636/1‑101 (MQ=255)


                                                 cTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                >  1:8114334/1‑101 (MQ=255)


                                                  tGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               >  2:4902477/1‑101 (MQ=255)


                                                  tGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               <  1:373586/101‑1 (MQ=255)


                                                   ggAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGcc                                              >  2:1812459/1‑101 (MQ=255)


                                                   ggAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGcc                                              >  2:3839711/1‑101 (MQ=255)


                                                    gAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                             >  1:1755547/1‑101 (MQ=255)


                                                     aaGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                            >  2:7502284/1‑101 (MQ=255)


                                                     aaGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                            <  2:9141556/101‑1 (MQ=255)


                                                          cGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcgg                                       >  2:1222048/1‑101 (MQ=255)


                                                          cGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcgg                                       >  1:5122486/1‑101 (MQ=255)


                                                           gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGACTTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  1:1664185/1‑101 (MQ=255)


                                                           gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGGTGTTCGACATTGCCGAGTCGCTCGCCTGgggggc                                      >  2:5777925/1‑101 (MQ=255)


                                                           gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  2:7213481/1‑101 (MQ=255)


                                                           gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  1:8634640/1‑101 (MQ=255)


                                                            tGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcg                                     <  1:150163/101‑1 (MQ=255)


                                                            tGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcg                                     <  2:8821556/101‑1 (MQ=255)


                                                              cTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcggc                                   >  1:3256015/1‑101 (MQ=255)


                                                               tggtggTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  >  2:3355336/1‑101 (MQ=255)


                                                                ggtggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                 >  1:6064861/1‑101 (MQ=255)


                                                                ggtggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                 <  1:7025262/101‑1 (MQ=255)


                                                                 gtggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCttt                                >  2:616717/1‑101 (MQ=255)


                                                                 gtggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCttt                                <  1:3839820/101‑1 (MQ=255)


                                                                  tggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                               <  1:3983284/101‑1 (MQ=255)


                                                                   ggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCg                              >  2:4165300/1‑101 (MQ=255)


                                                                    gtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                             <  1:692447/101‑1 (MQ=255)


                                                                    gtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                             >  2:676210/1‑101 (MQ=255)


                                                                      cGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                           >  2:8198823/1‑101 (MQ=255)


                                                                      cGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                           >  1:2235335/1‑101 (MQ=255)


                                                                        gagttcggggCCGCGCGCACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                         <  2:2979218/93‑1 (MQ=255)


                                                                        aaTTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                         >  1:5277017/1‑101 (MQ=255)


                                                                        aaTTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                         <  1:3394729/101‑1 (MQ=255)


                                                                         aTTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCg                        >  1:5953647/1‑101 (MQ=255)


                                                                         aTTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCg                        >  2:3605427/1‑101 (MQ=255)


                                                                         aTTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCg                        <  2:1299447/101‑1 (MQ=255)


                                                                           ttctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                      >  1:3021472/6‑101 (MQ=255)


                                                                           ttctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                      <  1:1911577/96‑1 (MQ=255)


                                                                            tctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                     >  2:4546533/5‑101 (MQ=255)


                                                                            tctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGCGa                     >  2:5357128/5‑101 (MQ=255)


                                                                             ctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    >  1:1065242/4‑101 (MQ=255)


                                                                             ctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    >  2:5847614/4‑101 (MQ=255)


                                                                              tgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   >  2:8003274/3‑101 (MQ=255)


                                                                              tgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGAGATg                   >  1:2825765/3‑101 (MQ=255)


                                                                                  ccGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCg               <  1:6236952/101‑1 (MQ=255)


                                                                                    gCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGcc             >  1:3496724/1‑101 (MQ=255)


                                                                                       cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc          >  1:127723/1‑101 (MQ=255)


                                                                                        gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  2:7584503/1‑101 (MQ=255)


                                                                                          aCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCgca       >  2:645378/1‑99 (MQ=255)


                                                                                           cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt      <  1:4811200/101‑1 (MQ=255)


                                                                                            gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg     <  2:8416704/101‑1 (MQ=255)


                                                                                             ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg    >  1:2719909/1‑101 (MQ=255)


AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GGCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG