Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,270 Δ1 bp 100% intergenic (+143/+34) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,2700A→.100.0% 116.7 393intergenic (+143/+34)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (241/121):  ref base (11/20):  total (252/141)
Fisher's exact test strand distribution p-value = 8.03e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.33e-01

Only 100 of 468 total aligned reads displayed.

CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCAAAAAAGGGGGGCGCGA.AGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGC  >  NC_014034/2044170‑2044367
                                                                                                     |                                                                                                  
cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGTGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCa                                                                                                    >  2:5911157/1‑101 (MQ=255)
cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCa                                                                                                    <  2:9075583/101‑1 (MQ=255)
cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCa                                                                                                    <  2:8851150/101‑1 (MQ=255)
  aaTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaa                                                                                                  >  1:52458/1‑101 (MQ=255)
   tccgatctCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaa                                                                                                 <  1:1542631/94‑1 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaa                                                                                                 <  1:6900186/101‑1 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaa                                                                                                 <  1:3473746/101‑1 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaaa                                                                                                <  1:7802468/101‑1 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaaa                                                                                                <  2:1663555/101‑1 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaaa                                                                                                <  2:4575570/101‑1 (MQ=255)
     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaaag                                                                                               >  2:5356719/1‑100 (MQ=255)
      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaaagg                                                                                              >  1:9171873/1‑99 (MQ=255)
      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaaagg                                                                                              >  2:718668/1‑99 (MQ=255)
      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCaaaaagg                                                                                              >  2:2723790/1‑99 (MQ=255)
         ttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCAAAAAGgggg                                                                                           >  2:8693042/1‑101 (MQ=255)
         ttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCAAAAAGgggg                                                                                           >  1:2421536/1‑101 (MQ=255)
         ttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCAAAAAGgggg                                                                                           >  2:4919604/1‑101 (MQ=255)
           cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCAAAAAGgggggc                                                                                         >  1:4635238/1‑100 (MQ=255)
              cTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGcgcg                                                                                     <  2:1480903/101‑1 (MQ=255)
              cTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGcgcg                                                                                     <  2:2297868/101‑1 (MQ=255)
               tCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                    >  2:8078276/1‑101 (MQ=255)
               tCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                    >  1:5763828/1‑101 (MQ=255)
                ccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGa.a                                                                                  >  1:5333670/1‑101 (MQ=255)
                ccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGa.a                                                                                  <  1:2662540/101‑1 (MQ=255)
                ccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGa.a                                                                                  <  2:5545310/101‑1 (MQ=255)
                 cccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.Ag                                                                                 <  1:277873/101‑1 (MQ=255)
                  ccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGc                                                                                <  1:8502852/101‑1 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGccccc                                                                            <  2:4514015/101‑1 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGccccc                                                                            <  1:3798819/101‑1 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGccccc                                                                            >  2:2578785/1‑101 (MQ=255)
                       ggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACCGC.AAACAGGGGGGTACGA.AGcccctc                                                                           >  2:2122200/1‑99 (MQ=255)
                       ggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGcccccc                                                                           >  1:6041365/1‑101 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCCtt                                                                         <  2:3787691/101‑1 (MQ=255)
                          tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCCttt                                                                        >  1:3513401/1‑101 (MQ=255)
                           atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCCtttt                                                                       >  2:7833382/1‑101 (MQ=255)
                           atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCCtttt                                                                       <  2:7330184/101‑1 (MQ=255)
                             ttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCCtttttc                                                                     >  2:6930518/1‑100 (MQ=255)
                               gAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCCtttttccg                                                                   >  1:8423114/1‑98 (MQ=255)
                                aGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCCtttttccgt                                                                  >  2:8635152/1‑97 (MQ=255)
                                 ggCAAGATAAAGACTGGCGAGGTGTTCTTCATCTTTCTGCAAAGCCCGCGGGGGAAA.TTTGCACAG.AAAAAGGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTc                                                                <  2:6326071/101‑1 (MQ=255)
                                    aaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGc                                                             >  2:6049751/1‑101 (MQ=255)
                                     aGATAAAGACTGGCGAGGTTTTCTTCATCTTTCTGAAAAGCCCGGGGGGGAAA.TTTGCACAG.AAAAAGGGGGGGCGCGA.A.CCCCCCCTTTTTCCGTCAGCg                                                            <  2:6673846/101‑1 (MQ=255)
                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGaa                                                          >  2:3186256/1‑101 (MQ=255)
                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGaa                                                          >  2:1350182/1‑101 (MQ=255)
                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCCGCAAAGCCGGGGGGGGGAA.TTTGCACAG.AAAAAGGGGGGGCGCGA.AGCCCCCC.TTTATCCGTCAGCGaa                                                          <  1:6583765/101‑1 (MQ=255)
                                        tAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGaaa                                                         >  1:1024336/1‑101 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAAc                                                        <  1:5093613/101‑1 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAAc                                                        >  2:1927690/1‑101 (MQ=255)
                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAg                                                      <  2:3288193/101‑1 (MQ=255)
                                            gACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGc                                                     <  2:2449702/101‑1 (MQ=255)
                                               ttGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCAcc                                                  <  1:1209242/101‑1 (MQ=255)
                                                  cGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgc                                               >  1:4520512/1‑101 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                              >  2:5097266/1‑101 (MQ=255)
                                                    aGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgc                                             >  1:9029818/1‑101 (MQ=255)
                                                     ggTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgcg                                            >  2:285597/1‑101 (MQ=255)
                                                      gtgtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgcgc                                           >  1:6475568/1‑101 (MQ=255)
                                                       tgtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGcc                                          >  1:5474013/1‑101 (MQ=255)
                                                         tttttcATCTTCCGGAAAAG.CCGGGGGGGGAATTTTGCAAAG.AAAAAGGGGGGGCGC.A.AACCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCgg                                        <  2:6372686/98‑1 (MQ=255)
                                                         ttcttcATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCgg                                        >  1:1830320/1‑101 (MQ=255)
                                                           cttcATCTTTCTGCAAAGACCGCGGGGGGAG.TTTGCACAGC.AAAAGGGGGGGCGCGA.A.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGc                                      <  1:8070247/101‑1 (MQ=255)
                                                            ttcATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCg                                     >  1:2504432/1‑101 (MQ=255)
                                                              cATGTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACGGCACCAGCGCGCCGGGCGAc                                   >  1:7215553/1‑101 (MQ=255)
                                                              cATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                   >  2:6823568/1‑101 (MQ=255)
                                                              cATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                   >  1:5053209/1‑101 (MQ=255)
                                                              cATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                   <  2:1221642/101‑1 (MQ=255)
                                                              cATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                   >  1:5887073/1‑101 (MQ=255)
                                                                tttttCAGCAAGCACCGGGGGGGAAA.TTGCCACAG.AAAAAGGGGGGGCGCGA.A.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCa                                 <  1:2201580/99‑1 (MQ=255)
                                                                  tttCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAgg                               >  1:1688237/1‑101 (MQ=255)
                                                                   ttCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGc                              >  1:1409001/1‑101 (MQ=255)
                                                                   ttCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGc                              >  1:2949329/1‑101 (MQ=255)
                                                                   ttCTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGc                              >  1:4465549/1‑101 (MQ=255)
                                                                     cTGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                            >  1:4154983/1‑101 (MQ=255)
                                                                      tGCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAc                           >  1:6692138/1‑101 (MQ=255)
                                                                       gCAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                          >  2:1825431/1‑101 (MQ=255)
                                                                        cAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAccc                         >  2:8397403/1‑101 (MQ=255)
                                                                        cAAAGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGAACCAGCGCGCCGGGCGACAAGGCCAccc                         >  2:4238106/1‑101 (MQ=255)
                                                                         aaaGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                        >  2:4731212/1‑101 (MQ=255)
                                                                         aaaGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                        >  2:161231/1‑101 (MQ=255)
                                                                          aaGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgc                       >  2:5068228/1‑101 (MQ=255)
                                                                          aaGACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgc                       >  2:387760/1‑101 (MQ=255)
                                                                            gACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGt                     >  1:1578049/1‑101 (MQ=255)
                                                                            gACCGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGt                     <  1:7312432/101‑1 (MQ=255)
                                                                              ccGGGGGGGGAA.TTTTCCAAAGAAAAAGGGGGGGCGCG..ACCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTc                    <  1:4780429/101‑1 (MQ=255)
                                                                              ccGCGGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCt                   <  2:5671043/101‑1 (MQ=255)
                                                                               cgcgGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTg                  <  1:7519628/101‑1 (MQ=255)
                                                                                cgggggggAATTTTGCACAGC.AAAAGGGGGGGCGCAA.CGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTg                  <  2:5793195/99‑1 (MQ=255)
                                                                                 cgGGGGGAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGGCCAGGCCACCCGCGTCTggg                >  1:5625452/1‑101 (MQ=255)
                                                                                  ggggggAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  1:3994001/1‑101 (MQ=255)
                                                                                  ggggggAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  1:7010046/1‑101 (MQ=255)
                                                                                     gggAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGcc            >  1:6261986/1‑101 (MQ=255)
                                                                                     gggAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGcc            <  2:3646154/101‑1 (MQ=255)
                                                                                     gggAA.TTGGCACAGC.AAAAGGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGcc            <  1:8693447/101‑1 (MQ=255)
                                                                                      ggAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCg           >  1:6181335/1‑101 (MQ=255)
                                                                                       gAA.TTTGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGa          >  2:5970296/1‑101 (MQ=255)
                                                                                        aa.TTGGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGAc         <  1:4242421/101‑1 (MQ=255)
                                                                                           aatttcgcacAAAAAAGGGGGGGGCGACACCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGa          <  1:8358968/92‑1 (MQ=255)
                                                                                            ttGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATc      >  2:6503599/1‑101 (MQ=255)
                                                                                            ttGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGCCTGGGTGCCGACATc      >  1:2992348/1‑101 (MQ=255)
                                                                                             tGCACAGC.AAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATcc     >  2:7659577/1‑101 (MQ=255)
                                                                                              gCACAG.AAAAAAGGGGGGCGCGA.AGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCa    <  1:3205524/101‑1 (MQ=255)
                                                                                                     |                                                                                                  
CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAA.TTTGCACAGCAAAAAAGGGGGGCGCGA.AGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGC  >  NC_014034/2044170‑2044367

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG