Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,123,403 Δ1 bp 97.6% coding (769/1077 nt) wza ← polysaccharide export protein Wza

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,123,4030A→.97.6% 28.6 0.0 ‑1.6416coding (769/1077 nt)wzapolysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  new base (199/207):  ref base (4/5):  total (204/212)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.39e-02

Only 100 of 433 total aligned reads displayed.

ACGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCG  >  NC_014034/2123303‑2123497
                                                                                                    |                                                                                               
  gAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGagg                                                                                               >  1:8364728/1‑99 (MQ=255)
   aaGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGgg                                                                                              >  1:4576861/1‑101 (MQ=255)
      aTGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                          >  2:7456268/1‑101 (MQ=255)
      aTGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                          <  2:6398725/101‑1 (MQ=255)
      aTGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                          >  2:2649252/1‑101 (MQ=255)
       tGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTg                                                                                         <  1:5650367/101‑1 (MQ=255)
        gCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGa                                                                                        >  1:4021769/1‑101 (MQ=255)
         ccGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAc                                                                                       <  1:767138/101‑1 (MQ=255)
         ccGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAc                                                                                       <  2:8043032/101‑1 (MQ=255)
             ggtcATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatc                                                                                   <  2:3175144/98‑5 (MQ=255)
              ggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatct                                                                                  <  1:5943604/101‑6 (MQ=255)
              ggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatct                                                                                  <  1:2542758/101‑6 (MQ=255)
              ggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatct                                                                                  >  1:4377310/1‑96 (MQ=255)
                cATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctgg                                                                                >  2:7076477/1‑94 (MQ=255)
                 aTCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggc                                                                               >  1:634440/1‑93 (MQ=255)
                  tCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggcc                                                                              >  2:6222720/1‑92 (MQ=255)
                   cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggccc                                                                             >  1:993648/1‑91 (MQ=255)
                   cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggccc                                                                             >  1:8683180/1‑91 (MQ=255)
                         tGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCcaagatc                                                                       >  1:2821771/1‑97 (MQ=255)
                           tCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATa                                                                     >  1:3518388/1‑101 (MQ=255)
                           tCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATa                                                                     <  1:1283569/101‑1 (MQ=255)
                            ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAg                                                                    <  1:3221344/101‑1 (MQ=255)
                            ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAg                                                                    <  1:6233917/101‑1 (MQ=255)
                            ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAg                                                                    <  2:4649823/101‑1 (MQ=255)
                            ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAg                                                                    <  2:6854631/101‑1 (MQ=255)
                             cTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAgg                                                                   >  2:4963551/1‑101 (MQ=255)
                             cTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAgg                                                                   >  1:8030616/1‑101 (MQ=255)
                              tCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGc                                                                  >  2:6573621/1‑101 (MQ=255)
                              gcGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGc                                                                  <  2:8819903/100‑1 (MQ=255)
                                ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcct                                                                <  2:2416218/101‑1 (MQ=255)
                                  ccAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGCGCCAGCAGATAGCgctcc                                                              >  2:2917116/1‑101 (MQ=255)
                                    aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTg                                                            <  1:5040188/101‑1 (MQ=255)
                                     gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGc                                                           <  2:5449475/101‑1 (MQ=255)
                                     gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGc                                                           >  1:1665127/1‑101 (MQ=255)
                                      aCCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCa                                                          <  2:3518094/101‑1 (MQ=255)
                                       ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                         >  2:4306450/1‑101 (MQ=255)
                                        cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                        <  2:5970976/101‑1 (MQ=255)
                                            gACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCt                                                    >  2:7312256/1‑101 (MQ=255)
                                            gACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCt                                                    >  2:752957/1‑101 (MQ=255)
                                            gACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCt                                                    <  2:634430/101‑1 (MQ=255)
                                             aCGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTg                                                   <  2:3300315/101‑1 (MQ=255)
                                              cgcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgc                                                  <  1:1120784/101‑1 (MQ=255)
                                                cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                >  2:8529388/1‑101 (MQ=255)
                                                  ggTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccgcc                                              >  1:3739005/1‑101 (MQ=255)
                                                   gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccgcca                                             >  2:2525615/1‑100 (MQ=255)
                                                   gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                             >  1:485279/1‑101 (MQ=255)
                                                   gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                             <  1:4680483/101‑1 (MQ=255)
                                                    tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                            <  1:2600528/101‑1 (MQ=255)
                                                    tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                            >  2:118720/1‑101 (MQ=255)
                                                    tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                            >  1:2880578/1‑101 (MQ=255)
                                                      aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                          <  2:5790195/101‑1 (MQ=255)
                                                      aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                          <  1:7947609/101‑1 (MQ=255)
                                                       tCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACg                                         <  1:7808430/101‑1 (MQ=255)
                                                          ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTTCCGCCCGACGCTg                                      >  1:3072005/1‑101 (MQ=255)
                                                          ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                      <  1:2473082/101‑1 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                     >  1:7418983/1‑101 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                     >  2:7859395/1‑101 (MQ=255)
                                                           cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTga                                     >  1:9094247/1‑101 (MQ=255)
                                                             gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                   <  2:1955604/101‑1 (MQ=255)
                                                             gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                   >  2:2215293/1‑101 (MQ=255)
                                                              ggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacga                                  >  1:614474/1‑101 (MQ=255)
                                                               gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                 <  1:6669556/101‑1 (MQ=255)
                                                               gTCAGGGTGATGTTTTCCAGCGTCAGACCGATCGCCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                 <  2:3643350/101‑1 (MQ=255)
                                                                 cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                               >  2:1429416/1‑101 (MQ=255)
                                                                 cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                               <  1:2007083/101‑1 (MQ=255)
                                                                 cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                               >  2:4782008/1‑101 (MQ=255)
                                                                  aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                              >  1:2317170/1‑101 (MQ=255)
                                                                   gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                             >  2:8292521/1‑101 (MQ=255)
                                                                   gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                             <  2:1572392/101‑1 (MQ=255)
                                                                   gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                             >  1:8914090/1‑101 (MQ=255)
                                                                         tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                       <  1:925550/101‑1 (MQ=255)
                                                                         tGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGttt                       <  2:568403/101‑1 (MQ=255)
                                                                          gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                      <  2:1855356/101‑1 (MQ=255)
                                                                          gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                      <  2:7141940/101‑1 (MQ=255)
                                                                          gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                      <  1:7156405/101‑1 (MQ=255)
                                                                               ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                 >  1:5325803/1‑101 (MQ=255)
                                                                                cAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagca                >  2:5170368/1‑101 (MQ=255)
                                                                                 aGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc               <  2:6716695/101‑1 (MQ=255)
                                                                                  gCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc              <  1:653790/101‑1 (MQ=255)
                                                                                   cGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCg             <  1:8827732/101‑1 (MQ=255)
                                                                                    gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATATGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg            <  1:6733841/101‑1 (MQ=255)
                                                                                    gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCGGTTTTGCAGCACCgg            >  1:4235538/1‑101 (MQ=255)
                                                                                    gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg            <  2:2880480/101‑1 (MQ=255)
                                                                                      cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt          >  1:1787593/1‑101 (MQ=255)
                                                                                      cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt          <  1:8191151/101‑1 (MQ=255)
                                                                                      cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt          >  2:3957661/1‑101 (MQ=255)
                                                                                      cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt          <  1:6043528/101‑1 (MQ=255)
                                                                                       cgatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt         <  2:1348745/100‑1 (MQ=255)
                                                                                       aGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt         <  1:1417892/101‑1 (MQ=255)
                                                                                       aGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt         >  2:5243899/1‑101 (MQ=255)
                                                                                        gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg        >  1:7496284/1‑101 (MQ=255)
                                                                                         atcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt       <  1:8600662/101‑1 (MQ=255)
                                                                                         atcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt       >  1:1200071/1‑101 (MQ=255)
                                                                                           cgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg     >  1:2171729/1‑101 (MQ=255)
                                                                                            gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg    <  2:4051609/101‑1 (MQ=255)
                                                                                            gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg    >  1:7292951/1‑101 (MQ=255)
                                                                                            gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg    >  2:8408468/1‑101 (MQ=255)
                                                                                            gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg    <  2:8682795/101‑1 (MQ=255)
                                                                                            gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg    <  1:1006406/101‑1 (MQ=255)
                                                                                             atcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgc   <  1:7012942/101‑1 (MQ=255)
                                                                                                    |                                                                                               
ACGAAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCG  >  NC_014034/2123303‑2123497

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG