Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,225 Δ1 bp 100% coding (914/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,2250A→.100.0% 1630.3 371coding (914/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (179/191):  ref base (0/0):  total (179/192)

Only 100 of 412 total aligned reads displayed.

CAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.C.GGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGC  >  NC_014034/2180127‑2180322
                                                                                                      |                                                                                                 
cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                                                                   <  2:5700493/101‑1 (MQ=255)
 aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGGCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                                                                  >  2:4423426/1‑100 (MQ=255)
 aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                                                                  >  1:9089624/1‑100 (MQ=255)
 aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                                                                  >  2:3255258/1‑100 (MQ=255)
 aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                                                                  >  1:275303/1‑100 (MQ=255)
 aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                                                                  >  1:2906510/1‑100 (MQ=255)
  aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                                                                 <  1:647599/101‑3 (MQ=255)
  aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                                                                 <  2:5937275/101‑3 (MQ=255)
   ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                                <  2:8049234/101‑4 (MQ=255)
    gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               >  1:735918/1‑97 (MQ=255)
    gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               <  1:1943163/101‑5 (MQ=255)
    gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               >  2:3567729/1‑97 (MQ=255)
    gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               <  1:5369450/101‑5 (MQ=255)
    aaCTCGGCCGTGGCCTATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.CGGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               <  1:2260354/100‑5 (MQ=255)
      cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                                                            <  1:6851008/101‑1 (MQ=255)
         ggCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcgg                                                                                         >  2:5764148/1‑101 (MQ=255)
          gCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCggg                                                                                        >  1:4918041/1‑101 (MQ=255)
          gCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCggg                                                                                        >  2:7269018/1‑101 (MQ=255)
           ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGGAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                                                       >  2:4219478/1‑101 (MQ=255)
            cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                                                                                      >  2:6003584/1‑101 (MQ=255)
             gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                                     <  1:7890298/101‑1 (MQ=255)
             gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                                     <  1:2199149/101‑1 (MQ=255)
              tGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                                                                    >  1:4745944/1‑101 (MQ=255)
                gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                                                                  <  2:3364210/101‑1 (MQ=255)
                  cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                                                                <  1:4978065/101‑1 (MQ=255)
                  cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGAACGCaaa                                                                                >  2:4517781/1‑101 (MQ=255)
                   gATCATGTCGGCTTCGCGCTGGCGCTGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                               <  2:271039/101‑1 (MQ=255)
                   gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                               >  1:7468194/1‑101 (MQ=255)
                   gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                               >  1:5153371/1‑101 (MQ=255)
                    aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                              <  1:3383340/101‑2 (MQ=255)
                     tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                                                             >  2:7564243/1‑99 (MQ=255)
                      cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                            >  1:8760414/1‑98 (MQ=255)
                      cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                            <  2:1568350/101‑4 (MQ=255)
                        tGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                         >  2:2033585/1‑101 (MQ=255)
                         gTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCgc                                                                        >  1:2812571/1‑100 (MQ=255)
                          tcggcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                                                                       <  1:6945249/101‑1 (MQ=255)
                           cggcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                      <  2:5193521/101‑1 (MQ=255)
                            ggcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                     >  2:5114613/1‑101 (MQ=255)
                             gcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                                                                    >  1:6754569/1‑101 (MQ=255)
                              cttcgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                                                                   >  1:1066837/7‑101 (MQ=255)
                               ttcgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcggc                                                                  <  2:2562935/96‑1 (MQ=255)
                                tcgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                                                                 <  1:7746182/97‑1 (MQ=255)
                                tcgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                                                                 <  1:1682430/97‑1 (MQ=255)
                                  gcgctgtcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                                                               >  1:5538340/10‑101 (MQ=255)
                                  gcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                                                               <  2:5340829/99‑1 (MQ=255)
                                  gcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCt                                                               >  2:3033827/3‑101 (MQ=255)
                                   cgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                                                              <  1:329468/100‑1 (MQ=255)
                                    gctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                                                             <  1:20562/101‑1 (MQ=255)
                                     ctggcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                            <  2:8876544/95‑1 (MQ=255)
                                         cgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGTCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                        <  2:7318589/99‑1 (MQ=255)
                                         cgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                        <  1:180428/99‑1 (MQ=255)
                                           ccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgt                                                      <  2:678634/101‑1 (MQ=255)
                                            cGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                     <  1:2595275/101‑1 (MQ=255)
                                            cGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                     <  2:2308237/101‑1 (MQ=255)
                                             gAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                                    <  2:4864041/101‑1 (MQ=255)
                                             gAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                                    >  1:4535508/1‑101 (MQ=255)
                                             gAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACG..AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   <  2:368564/101‑1 (MQ=255)
                                              aaCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   <  1:1232836/101‑1 (MQ=255)
                                              aaCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   >  2:7885464/1‑101 (MQ=255)
                                              aaCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   <  1:2367408/101‑1 (MQ=255)
                                                catggcc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                                 <  2:6460713/95‑1 (MQ=255)
                                                cAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcatcg                                                 <  2:8021494/101‑5 (MQ=255)
                                                  aggcc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                               >  1:428556/5‑101 (MQ=255)
                                                    gcc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGc                                             <  1:7623926/99‑1 (MQ=255)
                                                     cc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                            >  1:4152401/2‑101 (MQ=255)
                                                      c.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCgc                                           >  2:8385551/1‑100 (MQ=255)
                                                      c.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                           <  2:7047439/101‑1 (MQ=255)
                                                        ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                          >  2:8297588/1‑101 (MQ=255)
                                                        ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                          <  1:7269322/101‑1 (MQ=255)
                                                         gtgtCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAcc                                         >  2:1292554/1‑101 (MQ=255)
                                                          tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                        <  1:3916843/95‑1 (MQ=255)
                                                           gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                       >  2:6290465/6‑101 (MQ=255)
                                                            tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                                      <  2:6651513/97‑1 (MQ=255)
                                                            tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                                      <  1:3584918/97‑1 (MQ=255)
                                                            tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                                      <  1:8128557/97‑1 (MQ=255)
                                                            tcgcaaaGCATGGCAACTGGCTCGGGGCCTGGAA.CCTCGCC.TTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTAAAATTCGTCGAATGGGCGACCTcc                                      <  2:1692607/96‑1 (MQ=255)
                                                             cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     >  1:1377925/4‑101 (MQ=255)
                                                              gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCGGAAATTCACCGAGTGGGGCACCtacca                                    >  2:2943650/3‑97 (MQ=255)
                                                                cAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAgg                                  <  2:3815258/101‑1 (MQ=255)
                                                                 aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                                 <  1:5827406/101‑1 (MQ=255)
                                                                   gCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                               <  2:613023/101‑1 (MQ=255)
                                                                     aTGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTac                             <  2:2767916/101‑1 (MQ=255)
                                                                     aTGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTac                             <  1:7167910/101‑1 (MQ=255)
                                                                     aTGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTac                             >  2:1162662/1‑101 (MQ=255)
                                                                       ggCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTacac                           <  2:428548/101‑1 (MQ=255)
                                                                        gCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCTGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACAcc                          <  2:8654846/101‑1 (MQ=255)
                                                                        gCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACAcc                          >  1:61791/1‑101 (MQ=255)
                                                                          aaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGc                        >  2:1006162/1‑101 (MQ=255)
                                                                          aaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGc                        >  2:1851713/1‑101 (MQ=255)
                                                                            cTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGccc                      <  2:1377893/101‑1 (MQ=255)
                                                                              ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                    <  1:5288986/101‑1 (MQ=255)
                                                                                ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGGCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGt                  >  1:5966761/1‑101 (MQ=255)
                                                                                 tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTACAAGGACTACACCGCCCTTGTc                 <  1:8920115/101‑1 (MQ=255)
                                                                                   tGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGc               <  2:2138485/101‑1 (MQ=255)
                                                                                   tGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGc               >  1:8416929/1‑101 (MQ=255)
                                                                                      gCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAg            <  2:4644689/101‑1 (MQ=255)
                                                                                           ggaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaag        <  2:5538133/97‑1 (MQ=255)
                                                                                            gaCCCTCGCC.ATGCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaagg       <  1:3118347/100‑1 (MQ=255)
                                                                                               ccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaag    <  1:3669136/101‑1 (MQ=255)
                                                                                                cTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaagg   <  2:8796615/101‑1 (MQ=255)
                                                                                                      |                                                                                                 
CAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.C.GGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGC  >  NC_014034/2180127‑2180322

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG