Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,185,849 Δ1 bp 100% intergenic (+122/‑383) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,185,8490C→.100.0% 20.6 298intergenic (+122/‑383)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (62/224):  ref base (7/1):  total (72/226)
Fisher's exact test strand distribution p-value = 2.00e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.41e-05

Only 100 of 348 total aligned reads displayed.

ACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGG  >  NC_014034/2185751‑2185945
                                                                                                     |                                                                                                   
 cAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCAccc                                                                                                   <  2:8751181/101‑1 (MQ=255)
  aGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCAcccc                                                                                                  <  1:2469336/101‑1 (MQ=255)
   ggcggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCAccccc                                                                                                 <  1:4188302/101‑1 (MQ=255)
    gcggcCCCGCCCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCAcccccg                                                                                                <  1:451130/101‑2 (MQ=255)
     cggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGGGCCC.CCCCCCCcccccccc                                                                                              >  1:3378134/1‑99 (MQ=255)
      ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGGC.GGGGGGGCGG.CCCCCCCcccccccc                                                                                              >  2:7634368/1‑99 (MQ=255)
      ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGGTCCC.CCCCCCCACCCCCGGc                                                                                             >  2:8602096/1‑101 (MQ=255)
      ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGGGCCG.CCCCCCCCCCCCCCGc                                                                                             >  1:8565047/1‑101 (MQ=255)
       gcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGGTCCGCCCCCGCCCCCCCCgcc                                                                                             >  1:6996201/1‑99 (MQ=255)
        ccccGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGGGGCG.CCCCCCCCCCCCCCGccg                                                                                           >  2:2349703/1‑99 (MQ=255)
         cccGCTCCCTCCCGGGCGGGGCCGCCTGAGGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTa                                                                                          <  2:3524516/101‑1 (MQ=37)
          ccGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTAt                                                                                         <  2:6041796/101‑1 (MQ=255)
           cGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGGGCGCCC.CCCCCCCCCCCCCGCCggtt                                                                                         >  2:6274383/1‑98 (MQ=255)
            gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTAttt                                                                                       <  1:5306526/101‑1 (MQ=255)
            gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTAttt                                                                                       <  1:2761755/101‑1 (MQ=255)
            gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTAttt                                                                                       <  2:4376392/101‑1 (MQ=255)
              tccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTAt                                                                                     >  1:5877391/1‑101 (MQ=255)
               ccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATc                                                                                    >  2:1962221/1‑101 (MQ=255)
               ccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATc                                                                                    >  2:1925662/1‑101 (MQ=255)
                 ctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATccc                                                                                  <  1:6717686/101‑1 (MQ=255)
                  tcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCa                                                                                 <  1:325794/101‑1 (MQ=255)
                  ccccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCa                                                                                 <  2:4511079/100‑1 (MQ=37)
                   cccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCaa                                                                                <  1:5010623/101‑1 (MQ=255)
                    ccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAg                                                                               <  2:2768142/101‑1 (MQ=255)
                    ccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAg                                                                               <  1:896304/101‑1 (MQ=255)
                      gggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaa                                                                             <  2:8891196/101‑1 (MQ=255)
                      gggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaa                                                                             <  1:6071998/101‑1 (MQ=255)
                       ggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaaa                                                                            <  2:4037967/101‑1 (MQ=255)
                        gcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaaaa                                                                           <  1:6292349/101‑1 (MQ=255)
                        gcgggGCCGCCTGATCTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTCATCCCAAGaaaa                                                                           <  2:6698434/101‑1 (MQ=25)
                         cgggGCCGCCTGATGTGCGAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaaaaa                                                                          <  2:6567020/101‑1 (MQ=37)
                         cgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGaaaaa                                                                          <  2:3328892/101‑1 (MQ=255)
                          ggggCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAag                                                                         <  2:8262041/101‑1 (MQ=255)
                           gggCCGCCTGATGTGCAAAGAGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAATagc                                                                        <  1:7225092/101‑1 (MQ=25)
                              ccgccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAagcagc                                                                     <  1:1044795/101‑1 (MQ=255)
                                gccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCC.GCCCCCCCCCCCCCGCCCGCTTCTCCCCAGAAAAAACATCgg                                                                   >  1:1975252/1‑101 (MQ=255)
                                  cTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.t                                                                >  1:3930277/1‑101 (MQ=255)
                                   tGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.tt                                                               <  1:4857671/101‑1 (MQ=255)
                                   tGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.tt                                                               <  1:7634695/101‑1 (MQ=255)
                                    gATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTg                                                              <  2:5263518/101‑1 (MQ=255)
                                    gATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTg                                                              <  1:1276448/101‑1 (MQ=255)
                                     aTGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.c                                                            <  1:3694980/101‑1 (MQ=255)
                                        tgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTT.cccc                                                         >  2:661592/1‑101 (MQ=37)
                                        tgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.cccc                                                         <  2:4613450/101‑1 (MQ=255)
                                        tgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.cccc                                                         <  2:1352940/101‑1 (MQ=255)
                                        ggCAAAGCGGTTTCCCCNCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.cccc                                                         <  2:4226360/100‑1 (MQ=37)
                                         gCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.ccccc                                                        >  2:4858045/1‑100 (MQ=37)
                                          cAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTTG.ccccc                                                        >  2:1095455/1‑100 (MQ=25)
                                            aaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CGCCGCAg                                                     >  2:8780057/1‑101 (MQ=25)
                                             aGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAgg                                                    <  2:6328027/101‑1 (MQ=255)
                                                ggTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCACCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGAAGCGGC.TTT.cccccacgggct                                                 >  2:5029613/1‑93 (MQ=25)
                                                 gTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCtt                                                <  1:4741669/101‑1 (MQ=255)
                                                  tttGGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCCTg                                               >  1:8807462/1‑101 (MQ=25)
                                                   ttCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCCTGt                                              >  2:587117/1‑101 (MQ=37)
                                                    tCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGtt                                             <  2:4727475/101‑1 (MQ=255)
                                                     cGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGGTCCC.CCCCCCCACCCCCCGCGTCTTTATCCCAAGGGAAAGCAGCCGC.TTGTTCCCCAGGGCCTTGtt                                             >  2:7391823/1‑101 (MQ=255)
                                                     cGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGttt                                            <  2:7186453/101‑1 (MQ=255)
                                                     cGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGttt                                            <  1:4416704/101‑1 (MQ=255)
                                                      gCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGACGGGCTTgggtt                                           >  2:3636072/1‑97 (MQ=25)
                                                      gCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGtttt                                           <  2:8424100/101‑1 (MQ=255)
                                                       ccTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGttttg                                          <  2:2300877/101‑2 (MQ=37)
                                                       ccTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGttttg                                          <  1:6623490/101‑2 (MQ=37)
                                                        cTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGttttga                                         <  1:5584896/101‑3 (MQ=37)
                                                          cggcggTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGttttgatg                                       <  2:5797282/101‑5 (MQ=37)
                                                           ggcggTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATgg                                     <  1:892719/101‑1 (MQ=37)
                                                           ggcggTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCCAAGGCCTTG.TTTTGATgg                                     >  1:8390344/1‑101 (MQ=25)
                                                            gcggTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGc                                    <  1:3149765/101‑1 (MQ=37)
                                                            gcggTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGc                                    <  1:6468212/101‑1 (MQ=37)
                                                              ggTGCCTGGGCTGCCCT.GC.GGGGGCTCCG.CCCCCGCCCCCCCGGCGTATTTATCCCAAGAAAAACCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGcac                                  <  2:8958308/101‑1 (MQ=25)
                                                              ggTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGTTTTT.ATGGcac                                  >  1:5437922/1‑101 (MQ=37)
                                                               gTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGcaca                                 <  1:6841069/101‑1 (MQ=37)
                                                               gTGCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGcaca                                 <  1:6202262/101‑1 (MQ=37)
                                                                 gCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAg                               <  2:3073959/101‑1 (MQ=37)
                                                                 gCCGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAg                               <  1:2082181/101‑1 (MQ=37)
                                                                  ctgAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGa                              <  1:8280973/99‑1 (MQ=25)
                                                                  ccGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGa                              <  1:8072611/101‑1 (MQ=37)
                                                                  ccGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGa                              <  1:5821578/101‑1 (MQ=37)
                                                                   cGAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                             <  1:160550/101‑1 (MQ=37)
                                                                    gAGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGaaa                            <  1:1698800/101‑1 (MQ=37)
                                                                     aGGCGACTCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                           <  2:7810987/101‑1 (MQ=25)
                                                                     aGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                           <  2:5996190/101‑1 (MQ=37)
                                                                     aGGCGACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                           <  2:8015851/101‑1 (MQ=37)
                                                                         gACCCT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCg                       >  1:1703383/1‑101 (MQ=37)
                                                                             cT.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAcc                   <  2:7260769/101‑1 (MQ=37)
                                                                              t.GC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCg                  <  1:7972348/101‑1 (MQ=37)
                                                                                gC.GGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.c                <  2:1662913/101‑1 (MQ=37)
                                                                                   gggggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccc              >  2:6808705/1‑101 (MQ=37)
                                                                                   gggggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccc              <  1:9137619/101‑1 (MQ=37)
                                                                                   gggggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccc              >  1:6587872/1‑101 (MQ=37)
                                                                                   gggggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccc              >  1:379808/1‑101 (MQ=37)
                                                                                     gggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccccc            <  1:1768439/101‑2 (MQ=37)
                                                                                      ggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccccct           >  1:4721679/1‑99 (MQ=37)
                                                                                      ggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAACG.ccccct           >  1:753023/1‑99 (MQ=37)
                                                                                       gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctt          <  1:1449788/101‑1 (MQ=37)
                                                                                         tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctttc        <  2:701279/96‑3 (MQ=37)
                                                                                          ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCa        <  2:4962230/97‑1 (MQ=37)
                                                                                            gcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCAcc      <  1:1930835/99‑1 (MQ=38)
                                                                                             cccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCt     <  1:7761175/100‑1 (MQ=38)
                                                                                              ccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCtt    <  1:2880664/101‑1 (MQ=38)
                                                                                               cccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTg   <  1:2298103/101‑1 (MQ=38)
                                                                                                     |                                                                                                   
ACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GC.GGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGC.TTG.CCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGG  >  NC_014034/2185751‑2185945

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG