Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,236,666 Δ1 bp 1.6% intergenic (+17/‑112) RCAP_rcc02069 → / → RCAP_rcc02070 hypothetical protein/ArsR family transcriptional regulator/protein tyrosine phosphatase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,236,6660C→. 1.6% 6.0 ‑0.4 ‑0.0303intergenic (+17/‑112)RCAP_rcc02069/RCAP_rcc02070hypothetical protein/ArsR family transcriptional regulator/protein tyrosine phosphatase
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (163/135):  total (165/138)
Fisher's exact test strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.66e-01

Only 100 of 380 total aligned reads displayed.

GCCTGGGAACGCACGCTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGT  >  NC_014034/2236566‑2236766
                                                                                                      |                                                                                                    
gcCTGGGAACGCACGCTGGGCGAAGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.c                                                                                                      >  1:3961413/1‑101 (MQ=255)
  cTGGGAACGCACGCTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.ccc                                                                                                    >  2:6205690/1‑101 (MQ=255)
   tGGGAACGCACGCTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.cccc                                                                                                   >  1:7312477/1‑101 (MQ=255)
    gggAACGCGCGCTGGGCGATGCCGAGGTTTCCC.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.ccccc                                                                                                  <  2:2323551/101‑1 (MQ=255)
    gggAACGCACGCTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.ccccc                                                                                                  >  1:1877454/1‑101 (MQ=255)
     ggAACGCACGCTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.cccccc                                                                                                 >  1:2979628/1‑101 (MQ=255)
     ggAACGCACGCTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.cccccc                                                                                                 >  1:2460249/1‑101 (MQ=255)
       cacgcacgcTGGGCGATGCCGGGGTTTTCC.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.cccccccc                                                                                               <  2:4116411/100‑1 (MQ=255)
         cgcacgcGGGGCGGTCCCGGGGATTCCC.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCt                                                                                             <  1:1104166/101‑1 (MQ=255)
         cccccgcTGGGCGATGCCGAGGTTTCCC.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCt                                                                                             <  1:7942400/97‑1 (MQ=255)
          gcacgcTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTg                                                                                            >  1:4936791/1‑101 (MQ=255)
           cccgcGGGGCTATGCCGAGGTTTCCC.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGc                                                                                           <  2:1963153/99‑1 (MQ=255)
            ccgcTGGGCGAGCCCGAGGTTTCCC.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGcc                                                                                          <  1:4392402/100‑1 (MQ=255)
             cgcTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCCGccc                                                                                         >  1:102358/1‑101 (MQ=255)
                tGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGACGCCTG.CCCCCCCCCTGgccccc                                                                                      >  2:6637148/1‑100 (MQ=255)
                ggggCGGTCCCGAGGTTTTCC.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCg                                                                                      <  1:5960548/100‑1 (MQ=255)
                cggggggtGCCGGGGGTTTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGccccc                                                                                       <  1:8629877/94‑1 (MQ=255)
                  ggCGAGCCCGAGATTTCCC.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCa                                                                                    <  2:1061691/101‑1 (MQ=255)
                   gCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCaa                                                                                   >  1:5066879/1‑101 (MQ=255)
                    cGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAAc                                                                                  >  1:9070091/1‑101 (MQ=255)
                     gATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACg                                                                                 >  1:5742226/1‑101 (MQ=255)
                      aTGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACgg                                                                                >  1:7039246/1‑101 (MQ=255)
                      aTGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCCGCCCCCCCAACgg                                                                                >  2:1345066/1‑101 (MQ=255)
                          cGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGgcgc                                                                            >  1:8750950/1‑101 (MQ=255)
                           gAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCa                                                                           >  2:4930679/1‑101 (MQ=255)
                            aGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCCGAACGGGCGCAg                                                                          >  2:8371767/1‑101 (MQ=255)
                              gATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTc                                                                        >  2:847672/1‑101 (MQ=255)
                               gtttccccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTc                                                                        <  1:5851134/94‑1 (MQ=255)
                                 ttCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGc                                                                     >  1:7537368/1‑101 (MQ=255)
                                 ttCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGc                                                                     >  1:7084820/1‑101 (MQ=255)
                                  tCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGca                                                                    >  1:2631048/1‑101 (MQ=255)
                                  tCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCCGCCCCCGCCACCGGCGCCGTCTGcc                                                                    >  1:8195997/1‑100 (MQ=255)
                                   ccccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGca                                                                    <  1:2307320/98‑1 (MQ=255)
                                   cA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGgcgccgcctccca                                                                   >  2:8973334/1‑92 (MQ=255)
                                   cA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGcac                                                                   >  1:3658120/1‑101 (MQ=255)
                                      cccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAg                                                                 <  1:291650/101‑1 (MQ=255)
                                       ccgccgCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGt                                                                <  1:7795576/101‑1 (MQ=255)
                                        cgccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTc                                                               >  1:8881955/1‑101 (MQ=255)
                                          ccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGtaga                                                             >  2:2981847/1‑98 (MQ=255)
                                             cTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggcg                                                          >  2:2620455/1‑101 (MQ=255)
                                             cTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggcg                                                          >  1:4586513/1‑101 (MQ=255)
                                              ttCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggcgg                                                         >  2:3468478/1‑101 (MQ=255)
                                               tCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCggcggc                                                        >  1:3734011/1‑101 (MQ=255)
                                                 gAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGgcgc                                                      >  1:214034/1‑101 (MQ=255)
                                                  aaGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGcc                                                     <  1:4273473/101‑1 (MQ=255)
                                                   aGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCt                                                    >  1:3153377/1‑101 (MQ=255)
                                                   aGCTGCGCGGCTGGCGGGCCGGGCCGCGCCCTGCCGCGCCCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCt                                                    <  1:695930/101‑1 (MQ=255)
                                                    gctgcgctgcTGTCGGGCCGGGCCGCGCGCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTg                                                   >  1:6514144/1‑101 (MQ=255)
                                                     ctgcgctgcTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGt                                                  <  1:5704380/101‑1 (MQ=255)
                                                      cgccgtgctgGGGGGCCGGGCCGCCGCCTGGCGCGCCCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTc                                                 <  2:1204790/96‑1 (MQ=255)
                                                         gctgctGGGGGGCCGGGCCGCGCTCTGCCGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGcc                                              <  2:5034711/101‑1 (MQ=255)
                                                          gtgcggGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCg                                             <  2:1605159/100‑1 (MQ=255)
                                                          ctgctgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCg                                             <  2:5364868/101‑1 (MQ=255)
                                                           tgctgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCgg                                            >  2:313614/1‑101 (MQ=255)
                                                              tgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGAtt                                         >  2:3822396/1‑101 (MQ=255)
                                                               ggCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTg                                        <  2:1702366/101‑1 (MQ=255)
                                                               ggCGGGCCGGGCCGCGCGGTGACGCGCTCGGCGGCCTT.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTg                                        <  1:6637408/101‑1 (MQ=255)
                                                               ctctggccggctcgCCTTCGGACGCCCCCGGCCGCCCC.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCCCCGGAtag                                        <  2:4291075/97‑3 (MQ=255)
                                                                  gggcggggccGCGCTCTTACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCa                                     <  2:5963667/101‑1 (MQ=255)
                                                                   ggccgggccgCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCAt                                    >  2:6427235/1‑101 (MQ=255)
                                                                    gccgggccgCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATg                                   <  2:731293/101‑1 (MQ=255)
                                                                     ccgggccgCGTTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGa                                  <  1:1696994/101‑1 (MQ=255)
                                                                     ccgggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATg                                   >  2:3664329/1‑101 (MQ=255)
                                                                     ccgggccgCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGa                                  <  2:8839349/101‑1 (MQ=255)
                                                                      cgggccgGGCTCGNACGCGCTCNGCCGCCTN.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGaa                                 <  1:3388881/101‑1 (MQ=255)
                                                                      cgggccgCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGaa                                 >  2:586347/1‑101 (MQ=255)
                                                                       gggcccCGCCCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGaaa                                <  1:2021494/101‑1 (MQ=255)
                                                                       gagccgCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGAAACGAGCCCAGTCCGCACAGACCGGTGGCGCCTGTTGCCGGATTGCTATGaaa                                >  2:547039/3‑101 (MQ=255)
                                                                          ccgCGCCCTGACGCGCCCGGCCGCCTGCCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGc                              <  2:6331884/101‑1 (MQ=39)
                                                                           cgcgCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCtt                            >  1:5365362/1‑101 (MQ=255)
                                                                            gcgcTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTc                           <  1:3832808/101‑1 (MQ=255)
                                                                            gcgcTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTc                           <  2:449702/101‑1 (MQ=255)
                                                                            gcgcTCTGACGCGCTCGGCCGCCTG..CCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCa                          <  1:476296/101‑1 (MQ=255)
                                                                             cgcTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCa                          <  2:2206840/101‑1 (MQ=255)
                                                                               ctctGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACt                        <  2:3293894/101‑1 (MQ=255)
                                                                                tctGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTg                       >  1:2851072/1‑101 (MQ=255)
                                                                                tctGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTg                       <  1:8533477/101‑1 (MQ=255)
                                                                                  tGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGa                     <  2:2460176/101‑1 (MQ=255)
                                                                                   gACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGAc                    >  2:512131/1‑101 (MQ=255)
                                                                                   gACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGAc                    <  2:7084535/101‑1 (MQ=255)
                                                                                    aCGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACg                   >  1:6815661/1‑101 (MQ=255)
                                                                                    aCGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACg                   <  2:1437661/101‑1 (MQ=255)
                                                                                         cTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACgggggg              >  1:716275/1‑101 (MQ=255)
                                                                                         cTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACgggggg              <  1:6205929/101‑1 (MQ=255)
                                                                                           cGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAt            <  2:559102/101‑1 (MQ=255)
                                                                                           cGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAt            <  2:6780518/101‑1 (MQ=255)
                                                                                            ggCCGTCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAtt           <  2:459235/101‑1 (MQ=255)
                                                                                            ggCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAtt           >  2:5433098/1‑101 (MQ=255)
                                                                                            ggCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAtt           >  1:6359064/1‑101 (MQ=255)
                                                                                            ggCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGCGGGAtt           >  2:7646754/1‑101 (MQ=255)
                                                                                             gccgccTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTg          <  2:4074831/101‑1 (MQ=255)
                                                                                               cgccTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGCTTGCt        >  2:574684/1‑101 (MQ=255)
                                                                                               cgccTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCt        <  2:74479/101‑1 (MQ=255)
                                                                                                gccTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCtt       <  1:5565643/101‑1 (MQ=255)
                                                                                                gccGG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCtt       <  1:1274954/101‑1 (MQ=255)
                                                                                                  cTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGGTTGCTTcc     >  1:8459073/1‑101 (MQ=255)
                                                                                                   tG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCt    >  2:8029861/1‑101 (MQ=255)
                                                                                                    g.CCCCCCCCCTGCCCCCGCAACGGGCGCCGTCTGCACAGGCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGGCTCACTGGACGGGGGGAttagatcgga   >  2:8593565/1‑93 (MQ=25)
                                                                                                    g.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTcccg   >  2:8181739/1‑99 (MQ=255)
                                                                                                      cccccccccTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGt  <  1:5199504/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
GCCTGGGAACGCACGCTGGGCGATGCCGAGGATTTCA.CCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGT  >  NC_014034/2236566‑2236766

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG