Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,409,327 Δ1 bp 1.1% intergenic (‑208/+35) RCAP_rcc02237 ← / ← xsc radical SAM family protein/sulfoacetaldehyde acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,409,3270C→. 1.1% 3.3 ‑0.5 ‑0.3375intergenic (‑208/+35)RCAP_rcc02237/xscradical SAM family protein/sulfoacetaldehyde acetyltransferase
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (192/179):  total (195/180)
Fisher's exact test strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.56e-01

Only 100 of 446 total aligned reads displayed.

AGATGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGTTTCGTCATCGC  >  NC_014034/2409227‑2409427
                                                                                                    |                                                                                                    
aGATGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGc                                                                                                      >  2:6669819/1‑101 (MQ=255)
 gATGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcc                                                                                                     >  1:7464494/1‑101 (MQ=255)
 gATGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcc                                                                                                     >  2:5963878/1‑101 (MQ=255)
 gATGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcc                                                                                                     >  1:4030431/1‑101 (MQ=255)
 gATGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcc                                                                                                     <  2:7266113/101‑1 (MQ=255)
   tGGCACGGACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcccc                                                                                                   <  1:3482934/101‑1 (MQ=255)
   tGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcccc                                                                                                   <  1:5092540/101‑1 (MQ=255)
   tGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcccc                                                                                                   >  1:1966985/1‑101 (MQ=255)
   tGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcccc                                                                                                   <  2:2743997/101‑1 (MQ=255)
    ggCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGccccc                                                                                                  <  1:4568905/101‑1 (MQ=255)
     gCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcccccc                                                                                                 <  2:4662720/101‑1 (MQ=255)
      cACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGccccccc                                                                                                <  2:2514289/101‑1 (MQ=255)
       aCGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGcccccccc                                                                                               >  2:7394405/1‑101 (MQ=255)
        tgAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCg                                                                                              <  1:5420649/100‑1 (MQ=255)
         gAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCgg                                                                                             >  2:6997948/1‑101 (MQ=255)
         gAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCga                                                                                             >  2:5420464/1‑100 (MQ=255)
          aaCCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCggg                                                                                            >  2:5105421/1‑101 (MQ=255)
           aCCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGgc                                                                                           >  2:3002597/1‑101 (MQ=255)
             cccAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGgccg                                                                                         >  1:2220676/1‑101 (MQ=255)
                aGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGccgccc                                                                                      >  1:3579890/1‑101 (MQ=255)
                 gTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGccgcccg                                                                                     <  2:4966665/101‑1 (MQ=255)
                 gTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGccgcccg                                                                                     <  1:1003036/101‑1 (MQ=255)
                 gTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGccgcccg                                                                                     >  2:7505096/1‑101 (MQ=255)
                    cGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGa                                                                                  >  2:7952746/1‑101 (MQ=255)
                    cGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGa                                                                                  >  1:3027094/1‑101 (MQ=255)
                     ggTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGAc                                                                                 <  1:1540524/101‑1 (MQ=255)
                     ggTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGAc                                                                                 >  1:8568682/1‑101 (MQ=255)
                     ggTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGAc                                                                                 >  2:158822/1‑101 (MQ=255)
                       ttccattccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACgg                                                                               >  2:892563/1‑101 (MQ=255)
                        tccattccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGgc                                                                              <  2:1057984/101‑1 (MQ=255)
                        tccattccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGgc                                                                              >  2:4796507/1‑101 (MQ=255)
                        tccattccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGgc                                                                              >  1:1598071/1‑101 (MQ=255)
                         ccattccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGgcg                                                                             >  1:1369295/1‑101 (MQ=255)
                          cattccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGgcgc                                                                            >  2:8646429/1‑101 (MQ=255)
                           attccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCa                                                                           <  1:6550132/101‑1 (MQ=255)
                             tccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAgg                                                                         >  1:721293/1‑101 (MQ=255)
                             tccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAgg                                                                         <  2:7836633/101‑1 (MQ=255)
                              ccaAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGc                                                                        >  1:2902125/1‑101 (MQ=255)
                               caAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGcc                                                                       >  2:4005922/1‑101 (MQ=255)
                                  gTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAACAAGGATTATCCGCGGACCCCCCCGGGCCGCCCTCGACGTGTCCGGCCCGc                                                                    >  2:3885765/1‑101 (MQ=255)
                                    tGCCGCCCCCGCGGGGAAGGATCGCCCTGCGCGAACCCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTc                                                                  <  1:6670079/101‑1 (MQ=255)
                                     gCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCa                                                                 >  2:3558174/1‑101 (MQ=255)
                                     gCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCa                                                                 <  2:6145736/101‑1 (MQ=255)
                                     gCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCa                                                                 <  1:631750/101‑1 (MQ=255)
                                     gCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCa                                                                 <  1:6096331/101‑1 (MQ=255)
                                     gCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCa                                                                 >  1:3828172/1‑101 (MQ=255)
                                      ccGGCCCCGCGGGTAAGGATCGCCCTGCGCGAAGCCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAg                                                                <  1:2617010/101‑1 (MQ=255)
                                      ccGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGccgcgcagg                                                                >  1:8929889/1‑94 (MQ=255)
                                        ggCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAgcg                                                              <  1:3301941/101‑1 (MQ=255)
                                         gCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAgcgc                                                             <  1:1300355/101‑1 (MQ=255)
                                            ccGAGGGGAGGGATAACCGTGAGCGAAAGCGGCCTTGAAACAAGGATTTTCCGCGGCCCCCCCCGGCCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGc                                                          <  2:1498138/101‑1 (MQ=255)
                                               cgGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGc                                                       <  2:2674735/101‑1 (MQ=255)
                                                  ggAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGc                                                    >  1:5834692/1‑101 (MQ=255)
                                                   gAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGcc                                                   <  1:8374161/101‑1 (MQ=255)
                                                    aaGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCCGccc                                                  >  1:1745248/1‑100 (MQ=255)
                                                     aGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGc                                                 >  1:7780883/1‑101 (MQ=255)
                                                      ggATCACCCTGCGCGAAACCGGCCTTGAAGCACGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCa                                                <  2:6555909/101‑1 (MQ=255)
                                                       gATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGCTTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCAt                                               <  2:5901125/101‑1 (MQ=255)
                                                       gATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCAt                                               <  2:4331235/101‑1 (MQ=255)
                                                       gATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCAt                                               <  1:8735119/101‑1 (MQ=255)
                                                        aTCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATg                                              <  1:594549/101‑1 (MQ=255)
                                                        aTCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATg                                              <  1:6217551/101‑1 (MQ=255)
                                                          cGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGCCGGCGCAGGCCCGCTCAAGGCGGCTGGGGCCGCTTGTc                                            >  1:5634823/1‑101 (MQ=255)
                                                          cGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTc                                            <  1:9075218/101‑1 (MQ=255)
                                                           gCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCg                                           <  1:1867593/101‑1 (MQ=255)
                                                            cccTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCgg                                          >  2:5255166/1‑101 (MQ=255)
                                                                  gcgAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGgat                                    <  1:2402512/101‑1 (MQ=255)
                                                                  gcgAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGgat                                    <  1:422624/101‑1 (MQ=255)
                                                                  gcgAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGgat                                    >  2:6291248/1‑101 (MQ=255)
                                                                   cgAACCCGGCCTTGAACCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGgatc                                   <  2:3158473/101‑1 (MQ=255)
                                                                   cgAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGgatc                                   >  1:5274676/1‑101 (MQ=255)
                                                                    gAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGgatcg                                  >  1:8012293/1‑101 (MQ=255)
                                                                    gAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGgatcg                                  <  2:641031/101‑1 (MQ=255)
                                                                    gAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCCCAGCGCGGCTGCGGCCGCATGTCGGCCGgatcg                                  <  1:4772620/101‑1 (MQ=255)
                                                                     aaaCCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGAAGGCGCAGGCCCGCTCAGCGTGGCTGCGGCCGCATGTCGGCCGgatcga                                 >  1:6393367/1‑101 (MQ=255)
                                                                      acccGGCCTGGAAGCACGGATTTTCCGCGCCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGgatcgat                                <  2:3098835/99‑1 (MQ=255)
                                                                        ccGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATcc                              >  2:3380570/1‑101 (MQ=255)
                                                                          ggCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATcccc                            <  2:9103460/101‑1 (MQ=255)
                                                                          ggCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATcccc                            <  2:8294629/101‑1 (MQ=255)
                                                                          ggCCTTGAAGCAAGGATTCTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATcccc                            <  1:7540612/101‑1 (MQ=255)
                                                                             cTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCCTGTCGGCCGGATCGATCCCcgcg                         >  2:4285783/1‑101 (MQ=255)
                                                                               tGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCCCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCCCCTGTCGGCCGGGGCGAACCCCCCGAc                       >  2:413539/1‑101 (MQ=255)
                                                                                gAAGCGAGGATTTTCCGCGCCCCCCCCCGGGCCCCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGAcc                      <  2:8494158/101‑1 (MQ=255)
                                                                                 aaGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCa                     <  2:183646/101‑1 (MQ=255)
                                                                                  aGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCac                    <  2:7619429/101‑1 (MQ=255)
                                                                                   gCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCacg                   <  2:3827441/101‑1 (MQ=255)
                                                                                   gCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCacg                   <  2:2104330/101‑1 (MQ=255)
                                                                                     aaGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCacgac                 <  1:4971974/101‑1 (MQ=255)
                                                                                     aaGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCacgac                 >  1:388828/1‑101 (MQ=255)
                                                                                      aGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCaccacg                >  2:1270284/1‑101 (MQ=255)
                                                                                       ggATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCCCGACCACGACgg               >  2:6721089/1‑101 (MQ=255)
                                                                                         aTTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGt             >  1:6969603/1‑101 (MQ=255)
                                                                                          ttttCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGtt            <  1:6721357/101‑1 (MQ=255)
                                                                                           ttcccGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGttt           <  2:2220609/98‑1 (MQ=255)
                                                                                            ttCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGTTTc          <  1:4864032/101‑1 (MQ=255)
                                                                                            ttCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGTTTc          <  2:7467838/101‑1 (MQ=255)
                                                                                            ttCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGTTTc          <  2:5669754/101‑1 (MQ=255)
                                                                                             tCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGTTTCg         <  1:7877110/101‑1 (MQ=255)
                                                                                                gcgGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATACCCGCGACCACGACGGGTTTCGTca      <  1:7687134/101‑1 (MQ=255)
                                                                                                 cgGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGTTTCGTcat     <  1:6893346/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
AGATGGCACGAACCCCAGTCCGGTTCCATTCCAAGTTGCCGGCCCCGCGGGGAAGGATCGCCCTGCGCGAAACCGGCCTTGAAGCAAGGATTTTCCGCGGCCCCCCCCGGGCCGCCCGCGACGGCGCAGGCCCGCTCAGCGCGGCTGCGGCCGCATGTCGGCCGGATCGATCCCCGCGACCACGACGGGTTTCGTCATCGC  >  NC_014034/2409227‑2409427

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG