Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,420,306 Δ1 bp 1.3% intergenic (‑100/+92) RCAP_rcc02245 ← / ← cysE2 major membrane protein I/serine O‑acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,420,3060G→. 1.3% 6.0 ‑0.4 ‑0.5385intergenic (‑100/+92)RCAP_rcc02245/cysE2major membrane protein I/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (178/202):  total (181/204)
Fisher's exact test strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.04e-01

Only 100 of 392 total aligned reads displayed.

AGCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCTG  >  NC_014034/2420208‑2420395
                                                                                                  |                                                                                         
  ccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCa                                                                                <  1:8931671/101‑1 (MQ=255)
  ccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCa                                                                                <  1:4065260/101‑1 (MQ=255)
   cagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAt                                                                               <  2:9104820/101‑1 (MQ=255)
   cagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAt                                                                               >  2:6212743/1‑101 (MQ=255)
   cagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAt                                                                               <  1:5216251/101‑1 (MQ=255)
    agcGCGGC.GTGATCGTGTG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAtt                                                                              <  2:6672393/101‑1 (MQ=255)
     gcgcGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTc                                                                             <  2:7046171/101‑1 (MQ=255)
      cgcgGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCa                                                                            <  1:6699719/101‑1 (MQ=255)
      cgcgGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCa                                                                            <  1:2990384/101‑1 (MQ=255)
        gcggcgTGATCGTGGG.CAGGAT.CGGCG.CCGACTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCAc                                                                           >  1:5135788/6‑101 (MQ=255)
         cggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACg                                                                          <  1:1928907/97‑1 (MQ=255)
         cggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGGTCGGTCATTCACg                                                                          >  1:2319962/5‑101 (MQ=255)
          ggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGt                                                                         >  2:6814348/4‑101 (MQ=255)
          ggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGt                                                                         >  1:930009/4‑101 (MQ=255)
             gTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtct                                                                      >  2:2235726/1‑101 (MQ=255)
              tGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctc                                                                     >  1:1198846/1‑101 (MQ=255)
               gATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTcc                                                                    >  1:830058/1‑101 (MQ=255)
                  cGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCCGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttc                                                                 >  1:6086947/1‑101 (MQ=255)
                  cGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttc                                                                 <  1:6753008/101‑1 (MQ=255)
                  cGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttc                                                                 >  1:2541567/1‑101 (MQ=255)
                   gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct                                                                <  2:7466903/101‑1 (MQ=255)
                   gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct                                                                >  2:3205336/1‑101 (MQ=255)
                   gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct                                                                <  2:9021436/101‑1 (MQ=255)
                     tgggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttctt                                                               >  1:3814777/5‑101 (MQ=255)
                        gggagaatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCGGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgg                                                             <  2:2897436/93‑1 (MQ=255)
                        gcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTggg                                                            >  2:3161287/2‑101 (MQ=255)
                         cAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggg                                                           >  1:3586219/1‑101 (MQ=255)
                           aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtg                                                          >  1:2746934/6‑99 (MQ=255)
                           aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtg                                                          <  1:5345702/96‑3 (MQ=255)
                               tcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGa                                                     <  1:4512367/100‑1 (MQ=255)
                                cGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaa                                                    <  1:8586574/101‑1 (MQ=255)
                                  ggcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaa                                                   >  2:5723390/5‑101 (MQ=255)
                                     gccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAAcc                                                <  1:8714878/100‑1 (MQ=255)
                                      gcGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCa                                               <  2:5942688/100‑1 (MQ=255)
                                      ccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCa                                               <  1:7458511/101‑1 (MQ=255)
                                       cGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCAt                                              <  2:726472/101‑1 (MQ=255)
                                       cGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCAt                                              >  1:4159383/1‑101 (MQ=255)
                                        gTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATc                                             <  1:148920/101‑1 (MQ=255)
                                         tCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATcc                                            <  1:5677578/101‑1 (MQ=255)
                                          cTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATccc                                           <  1:4653790/101‑1 (MQ=255)
                                          cTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATccc                                           >  1:6625596/1‑101 (MQ=255)
                                          cTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATccc                                           >  2:6966205/1‑101 (MQ=255)
                                           ttCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcg                                          <  2:3712433/101‑1 (MQ=255)
                                            tCGTGGCATTGGC.AAGCTGGCGGGGGGGACTTGCGGCCCGCGCCAG.TTGAC.GCGGGTCGGGCCTTCACGTTCTCCTTCTTGGGGGGC.GAAAACCATCCcgc                                         >  2:8155452/1‑101 (MQ=255)
                                            tCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgc                                         <  1:7111678/101‑1 (MQ=255)
                                            tCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgc                                         <  1:1857286/101‑1 (MQ=255)
                                              gTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCgg                                       >  1:807477/1‑101 (MQ=255)
                                              gTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCgg                                       >  2:3370822/1‑101 (MQ=255)
                                               tGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGa                                      >  1:6409457/1‑101 (MQ=255)
                                                ggCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAc                                     <  1:3712689/101‑1 (MQ=255)
                                                ggCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAc                                     <  2:8703271/101‑1 (MQ=255)
                                                ggCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAc                                     >  1:3097430/1‑101 (MQ=255)
                                                ggCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAc                                     >  2:4014475/1‑101 (MQ=255)
                                                  cATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcgc                                   >  1:1676422/1‑101 (MQ=255)
                                                    ttGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACgcgc                                 >  1:394729/1‑101 (MQ=255)
                                                      tggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgcc                                <  2:1716862/97‑1 (MQ=255)
                                                       ggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgccg                               >  2:4853460/4‑101 (MQ=255)
                                                       ggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgccg                               >  2:8409323/4‑101 (MQ=255)
                                                        gcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgccgc                              >  1:7839063/3‑101 (MQ=255)
                                                         caaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCcgcg                             <  1:1304273/100‑1 (MQ=255)
                                                          aaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCgg                            <  2:3814668/101‑1 (MQ=255)
                                                           aGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCggg                           <  2:4360105/101‑1 (MQ=255)
                                                           aGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCggg                           <  1:2068413/101‑1 (MQ=255)
                                                            gCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCCGGa                          <  1:7217531/101‑1 (MQ=255)
                                                             cTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAt                         <  2:6109956/101‑1 (MQ=255)
                                                             cTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAt                         >  2:5654325/1‑101 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        <  2:3548747/101‑1 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        <  2:6409538/101‑1 (MQ=255)
                                                              tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        >  2:6899107/1‑101 (MQ=255)
                                                               ggcgggcgGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTg                       >  2:6749404/1‑101 (MQ=255)
                                                               ggcgggcgGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTg                       <  1:6917861/101‑1 (MQ=255)
                                                                  gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    >  1:4768786/1‑101 (MQ=255)
                                                                  gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    >  2:242965/1‑101 (MQ=255)
                                                                  gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    >  2:6603138/1‑101 (MQ=255)
                                                                   ggcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAg                   <  1:7621895/101‑1 (MQ=255)
                                                                   ggcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAg                   <  1:3474825/101‑1 (MQ=255)
                                                                   ggcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAg                   <  2:5471766/101‑1 (MQ=255)
                                                                     cggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGt                 <  1:8155801/101‑1 (MQ=255)
                                                                      ggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAgggt                >  2:1264568/1‑99 (MQ=255)
                                                                       gcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTg               >  2:5054022/1‑101 (MQ=255)
                                                                        cACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGa              <  2:4169042/101‑1 (MQ=255)
                                                                         aCTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTTaa             >  1:3309425/1‑101 (MQ=255)
                                                                         aCTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaa             >  2:4644016/1‑101 (MQ=255)
                                                                         aCTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaa             <  2:6062205/101‑1 (MQ=255)
                                                                         aCTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaa             <  2:1545758/101‑1 (MQ=255)
                                                                         aCTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaa             >  1:909379/1‑101 (MQ=255)
                                                                          cTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGGTGaaa            >  2:775807/1‑101 (MQ=255)
                                                                             tCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAtg         <  2:1116496/101‑1 (MQ=255)
                                                                                cccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATgtgt      >  1:1897211/1‑101 (MQ=255)
                                                                                 ccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGtt     <  2:830045/101‑1 (MQ=255)
                                                                                 ccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGtt     <  2:2162705/101‑1 (MQ=255)
                                                                                 ccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGtt     >  2:1365596/1‑101 (MQ=255)
                                                                                 ccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGtt     >  1:8764765/1‑101 (MQ=255)
                                                                                 ccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGtt     <  1:599988/101‑1 (MQ=255)
                                                                                  cAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTc    <  2:1814686/101‑1 (MQ=255)
                                                                                   aGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCt   <  1:1042269/101‑1 (MQ=255)
                                                                                   aGCGCCAG.TTGAC.GCGGGTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCt   <  2:5168352/101‑1 (MQ=255)
                                                                                    gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCtg  <  2:4508279/101‑1 (MQ=255)
                                                                                    gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCtg  >  2:4118313/1‑101 (MQ=255)
                                                                                    gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCtg  >  1:5527565/1‑101 (MQ=255)
                                                                                                  |                                                                                         
AGCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCTG  >  NC_014034/2420208‑2420395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG