Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,435,309 Δ1 bp 2.3% intergenic (+18/‑36) RCAP_rcc02259 → / → RCAP_rcc02260 WbqC‑like protein family/type 11 family methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,435,3090C→. 2.3% 15.1 ‑0.7 ‑0.3346intergenic (+18/‑36)RCAP_rcc02259/RCAP_rcc02260WbqC‑like protein family/type 11 family methyltransferase
Reads supporting (aligned to +/- strand):  new base (6/2):  ref base (198/140):  total (204/142)
Fisher's exact test strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.54e-01

Only 100 of 453 total aligned reads displayed.

ATACAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCATGC  >  NC_014034/2435209‑2435409
                                                                                                     |                                                                                                    
ataCAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.c                                                                                                      >  1:5706043/1‑101 (MQ=255)
   cAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.cccc                                                                                                   <  1:5316168/101‑1 (MQ=255)
     ggAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.cccccc                                                                                                 <  2:3606069/101‑1 (MQ=255)
      gAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.ccccccc                                                                                                >  2:4619181/1‑101 (MQ=255)
      gAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.ccccccc                                                                                                >  1:7958365/1‑101 (MQ=255)
       aaaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.cccccccc                                                                                               <  1:3926822/101‑1 (MQ=255)
        aaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.ccccccccc                                                                                              >  1:6003995/1‑101 (MQ=255)
        aaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.ccccccccc                                                                                              >  1:8145018/1‑101 (MQ=255)
         aaCATACATTTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.cccccccccc                                                                                             <  2:4054093/101‑1 (MQ=255)
          acatacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCg                                                                                            >  2:7562097/1‑101 (MQ=255)
          acatacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCg                                                                                            >  1:1531382/1‑101 (MQ=255)
            atacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCgga                                                                                          >  2:727327/1‑99 (MQ=255)
            atacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGaa                                                                                          >  1:8240630/1‑101 (MQ=255)
                attttttCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATc                                                                                      <  1:7526407/98‑1 (MQ=255)
                   ttttCTCCCCGCCCTGTTCGAACGGACCCTGGCGGGGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATcccc                                                                                   <  1:1415394/101‑1 (MQ=255)
                    tttCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCg                                                                                  >  1:6411670/1‑101 (MQ=255)
                    tttCTGCCCCCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCg                                                                                  <  1:2425525/101‑1 (MQ=255)
                     ttCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAACCCCCGa                                                                                 >  2:7455301/1‑101 (MQ=255)
                      tCTGCCCGCCCTGTTCGAACGGACCGTGGAGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAg                                                                                <  2:648197/101‑1 (MQ=255)
                      tCTGCCCGCCCTGATCTAACAGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAg                                                                                <  1:3202617/101‑1 (MQ=255)
                       cTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGc                                                                               <  2:3688950/101‑1 (MQ=255)
                       cTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCGAGc                                                                               >  1:2765884/1‑101 (MQ=255)
                       cTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTC.CCCACCCCCCCAAAAACTCCGatc                                                                               >  2:2221628/1‑99 (MQ=255)
                        tGCCCGCCCTGTTCGAACGGCCCGTGGGGGAGTTGCGTGCAGACATCTTGGAAGGCTGCCCCTTCCGATCCGTGTGCCCCCCCCCCCGAAAATCCCCGAGc                                                                               <  2:4501664/101‑1 (MQ=255)
                         gcccgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCCAGCgg                                                                             >  1:4875975/1‑101 (MQ=255)
                          cccgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGga                                                                            >  2:848567/1‑101 (MQ=255)
                            cgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGgaga                                                                          <  1:8659664/101‑1 (MQ=255)
                             gcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGCGCGGAGaa                                                                         >  1:422376/1‑101 (MQ=255)
                             gcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAAAccccaaccgcggaa                                                                         >  1:7632243/1‑91 (MQ=255)
                              cccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAg                                                                        <  1:957413/101‑1 (MQ=255)
                               ccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGc                                                                       <  2:6832635/101‑1 (MQ=255)
                               ccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGACCGAAGAAGc                                                                       >  1:1616064/1‑101 (MQ=255)
                                 tGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCCAGCGTAGAAACgc                                                                     >  1:6833622/1‑100 (MQ=255)
                                  tgtCGAACGGGCCGGGGCGGAGGTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCCGGGG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTc                                                                    <  2:620903/99‑1 (MQ=255)
                                  gTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTc                                                                    >  2:5080456/1‑101 (MQ=255)
                                  gTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTc                                                                    >  1:1747286/1‑101 (MQ=255)
                                   ttCGAACGGACCGGGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTcc                                                                   <  2:7385691/101‑1 (MQ=255)
                                      gAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATg                                                                <  1:4175690/101‑1 (MQ=255)
                                       aaCGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGGGAAGCGTcacagg                                                               >  2:5202705/1‑96 (MQ=255)
                                        aCGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG..CCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGcc                                                             <  2:2327275/101‑1 (MQ=255)
                                          ggACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGGGAAGCGTCCAGGGccc                                                            >  1:7343683/1‑101 (MQ=255)
                                          ggACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAAACCCCGAGCGCAGAAGGGCCCAAGGgcc                                                            >  2:3902473/1‑101 (MQ=255)
                                            cccGGGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCa                                                          <  2:6633930/100‑1 (MQ=255)
                                             ccGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCaa                                                         >  2:1869026/1‑101 (MQ=255)
                                             ccGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCaa                                                         <  2:1594732/101‑1 (MQ=255)
                                              cGTGGCGGAGTTGCGTGCAGACATATTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGCGCGGAGAAGCGGCCATGGCCCCAAg                                                        >  2:4376566/1‑101 (MQ=255)
                                               gTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGc                                                       <  2:6099630/101‑1 (MQ=255)
                                               gTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAACCGCCCATGGCCCCCAGc                                                       >  2:593172/1‑101 (MQ=255)
                                                 ggCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGa                                                     >  2:5546754/1‑101 (MQ=255)
                                                   cGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGAcc                                                   <  1:7879336/101‑1 (MQ=255)
                                                    ggAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGAccc                                                  >  1:7195510/1‑101 (MQ=255)
                                                    ggAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGAccc                                                  >  1:4976164/1‑101 (MQ=255)
                                                    ggAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGAccc                                                  >  1:6191809/1‑101 (MQ=255)
                                                    ggAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCGAGCGGCGAAGCGCCCCTGGCCCCAAGCGAccc                                                  >  1:203342/1‑101 (MQ=255)
                                                      ggTTGCGTGCAGACATCTTGGAAGGCCGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCg                                                <  2:3133347/100‑1 (MQ=255)
                                                      aGTTGCGTGCATACATCTTGGAAGGCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCg                                                <  2:2813493/101‑1 (MQ=255)
                                                        tgccgccctgACCTCTTGGGGGGCTCACCCTTCGCATCCGTGGC.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGCCCATGGCCCCAAGCGACCCCGGt                                              <  2:1241199/92‑1 (MQ=255)
                                                            gTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCCACCCCGGTGacc                                          >  2:1397948/1‑100 (MQ=255)
                                                            gTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGCCCATGGCCCCAAGCGACCCCGGTGacc                                          >  2:7845762/1‑100 (MQ=255)
                                                              gCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACAcc                                        <  1:8290502/101‑1 (MQ=255)
                                                              gCAGACATCTTGGAAGCCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACAcc                                        <  2:4875815/101‑1 (MQ=255)
                                                                aGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGGGAAGCGTCCATGGCCCCCACCGACCCCGGCGCCACCgg                                      >  1:1335700/1‑100 (MQ=255)
                                                                aGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGt                                      >  2:7021239/1‑101 (MQ=255)
                                                                aGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAAACCCCCGGGGGGGAAGCGGCCATGGGCCCAAACCACCCCGGGGGCAcccg                                      >  1:6276711/1‑99 (MQ=255)
                                                                 gcgaTCTTGGAAGCCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTg                                     <  2:2460025/98‑1 (MQ=255)
                                                                 gACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTg                                     >  1:5075947/1‑101 (MQ=255)
                                                                 gACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCGAGCGGGGAAGCGGCCATGGCCCCAAGCGACCCCCGTGGCACCGTg                                     >  2:7881588/1‑101 (MQ=255)
                                                                    ctCTTGGAAGCCTGCCCCTGCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATg                                  <  1:8962646/100‑1 (MQ=255)
                                                                    aTCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGACACCGCGATg                                  >  1:5794614/1‑101 (MQ=255)
                                                                      cTTGGAAGCCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATgcc                                <  2:3847424/101‑1 (MQ=255)
                                                                        tGGAAGGCTGACCCTTCCGATCCGTGGG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATgccgc                              <  2:5418658/101‑1 (MQ=255)
                                                                        gggggggCTGACCCTTGTGTGCCGGGGG.CCCCCCGCCGGAAAACCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGGCCCCGGTGACCCCGTGATgccgc                              <  2:4244714/96‑1 (MQ=255)
                                                                          gacgccTGCCCCTCCGGAGCCGGGTG..CCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGccgcccg                           <  2:2012049/96‑1 (MQ=255)
                                                                          gAAGGCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGccgccc                            <  1:1018226/101‑1 (MQ=255)
                                                                          gAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGACACCGCGATGccgccc                            >  1:3466082/1‑101 (MQ=255)
                                                                              gCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCt                        >  2:3048954/1‑101 (MQ=255)
                                                                               cTGACCCTTCCGATCCGTGTG..CCCCCCCCCGAAAAACCCCCAACGGAGAAACGACCAAGGCCCCAAACCACCCCCGGGACACCCGGAAGGCCCCCGCCCtc                      >  1:2207673/1‑101 (MQ=255)
                                                                                tGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtc                      <  1:5211663/101‑1 (MQ=255)
                                                                                gagcccTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtc                      <  2:8970627/98‑1 (MQ=255)
                                                                                 gACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGACACCGTGATGCCGCCCGCCTtcg                     >  1:4327485/1‑101 (MQ=255)
                                                                                   cccTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcgtc                   <  2:5768539/101‑1 (MQ=255)
                                                                                    ccTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcgtcg                  <  2:812420/101‑1 (MQ=255)
                                                                                      ttCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGGGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGAGACCGGGGTGCCGACCGCCTtcgtcggt                >  2:8370229/1‑99 (MQ=255)
                                                                                       tCCGATCCGTGTGCCCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGAt                >  1:6725880/1‑101 (MQ=255)
                                                                                       tCCGATCCGTGTC.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATc               <  1:6500321/101‑1 (MQ=255)
                                                                                        ccGATCCGTGTGCCCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATc               <  1:4744201/101‑1 (MQ=255)
                                                                                        ccGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATcc              <  2:8691634/101‑1 (MQ=255)
                                                                                         cGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCg             <  2:4772226/101‑1 (MQ=255)
                                                                                         cGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCg             <  1:4428636/101‑1 (MQ=255)
                                                                                          gATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGc            >  2:8753358/1‑101 (MQ=255)
                                                                                           aTCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGcc           >  1:8505512/1‑100 (MQ=255)
                                                                                           aTCCGTGTC.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCt           <  1:2996401/101‑1 (MQ=255)
                                                                                           aTCCGAGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCt           >  1:5449965/1‑101 (MQ=255)
                                                                                             ccGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCg         <  1:363214/101‑1 (MQ=255)
                                                                                             ccGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCg         >  2:8075325/1‑101 (MQ=255)
                                                                                               gtgtG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGc       <  1:2290033/101‑1 (MQ=255)
                                                                                                tgtg.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGcc      >  2:3294258/1‑101 (MQ=255)
                                                                                                tgtg.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGcc      >  1:4604604/1‑101 (MQ=255)
                                                                                                 gtg.CCCCCCCCCCGAAAATCCCCGAGCGGGGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGccc     >  2:5714933/1‑100 (MQ=255)
                                                                                                  gtcccccccccccGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCa     <  1:1903520/98‑1 (MQ=255)
                                                                                                     |                                                                                                    
ATACAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCATGC  >  NC_014034/2435209‑2435409

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG