Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,470,253 Δ1 bp 96.0% intergenic (‑186/+31) RCAP_rcc02294 ← / ← proB signal transduction histidine kinase/glutamate 5‑kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,470,2530G→.96.0% 35.9 ‑0.9 ‑0.2350intergenic (‑186/+31)RCAP_rcc02294/proBsignal transduction histidine kinase/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  new base (141/195):  ref base (4/10):  total (145/205)
Fisher's exact test strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.44e-01

Only 100 of 390 total aligned reads displayed.

CCTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTC  >  NC_014034/2470153‑2470349
                                                                                                    |                                                                                                
ccTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAg                                                                                                  >  1:8429889/1‑101 (MQ=255)
ccTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAg                                                                                                  >  2:6340879/1‑101 (MQ=255)
 cTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgg                                                                                                 >  2:7674127/1‑101 (MQ=255)
 cTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgg                                                                                                 <  2:3571922/101‑1 (MQ=255)
      ttCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAggggggg                                                                                            >  1:2818934/1‑101 (MQ=255)
        cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          <  1:5305500/101‑3 (MQ=255)
        cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          <  2:4998473/101‑3 (MQ=255)
        cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          >  1:1959749/1‑99 (MQ=255)
         ggCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcc                                                                                         >  2:1120033/1‑101 (MQ=255)
          gcgcGGGGGCGTTGATGCCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcct                                                                                        <  1:2928296/101‑2 (MQ=255)
          gcgcGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcct                                                                                        <  1:2159777/101‑2 (MQ=255)
          gcgcGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcct                                                                                        >  2:7273627/1‑100 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  1:1744446/101‑1 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  2:1709973/101‑1 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  1:729028/101‑1 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  2:9101310/101‑1 (MQ=255)
            gcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGc                                                                                     <  2:4620844/101‑1 (MQ=255)
              ggggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccc                                                                                   >  1:7754860/1‑101 (MQ=255)
               gggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccc                                                                                  >  1:6304588/1‑101 (MQ=255)
               gggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccc                                                                                  >  1:1009535/1‑101 (MQ=255)
                ggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccccc                                                                                 <  1:3187102/101‑1 (MQ=255)
                ggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccccc                                                                                 <  2:2720250/101‑1 (MQ=255)
                 gACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccccc                                                                                >  1:1264286/1‑101 (MQ=255)
                 gACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccccc                                                                                <  1:6083639/101‑1 (MQ=255)
                  gcGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCct                                                                               <  2:8819167/100‑1 (MQ=255)
                  aCGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCct                                                                               <  1:4821575/101‑1 (MQ=255)
                  aCGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCct                                                                               <  1:5209697/101‑1 (MQ=255)
                   cGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctc                                                                              <  1:6797935/101‑1 (MQ=255)
                   cGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctc                                                                              <  1:1118942/101‑1 (MQ=255)
                   cGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctc                                                                              <  2:2181451/101‑1 (MQ=255)
                    gTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctct                                                                             <  2:3280278/101‑1 (MQ=255)
                     ttGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTg                                                                            <  2:4120275/101‑1 (MQ=255)
                      tgatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGc                                                                           <  2:2331696/101‑1 (MQ=255)
                      tgatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGc                                                                           >  1:2355550/1‑101 (MQ=255)
                       gatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAACCCCGGGCGTTGATGACACCAAA.CGGGGGGCCCTGCCCCCctacgcc                                                                          >  2:2399376/1‑96 (MQ=255)
                        atgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGccc                                                                         >  1:2550717/1‑101 (MQ=255)
                         tgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGcccc                                                                        <  1:6383866/101‑1 (MQ=255)
                               gcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTc                                                                  <  1:5468373/101‑1 (MQ=255)
                                cgGGCGCCAAAGGAACGCGCCCGGATCAACGTCGCCCGCGCGCAAACCCGGGTGTTGATGAACCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 <  1:5864273/101‑1 (MQ=255)
                                cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 >  1:7128098/1‑101 (MQ=255)
                                 gggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCTCGGCCTCAg                                                                <  1:8803933/101‑1 (MQ=255)
                                   gcgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGcc                                                              <  1:8611369/101‑1 (MQ=255)
                                      ccAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGAt                                                           >  1:7817157/1‑101 (MQ=255)
                                      ccAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGAt                                                           <  1:4612245/101‑1 (MQ=255)
                                       cAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATc                                                          <  1:2597796/101‑1 (MQ=255)
                                       cAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATc                                                          >  1:4081788/1‑101 (MQ=255)
                                       cAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATc                                                          <  1:5121378/101‑1 (MQ=255)
                                        aaaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCa                                                         >  2:3455520/1‑101 (MQ=255)
                                        aaaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCa                                                         >  2:5077135/1‑101 (MQ=255)
                                        aaaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCa                                                         <  2:8989519/101‑1 (MQ=255)
                                        aaaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCa                                                         >  1:4530907/1‑101 (MQ=255)
                                         aaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAc                                                        >  2:8414917/1‑101 (MQ=255)
                                         aaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAc                                                        >  1:9106829/1‑101 (MQ=255)
                                          aGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAcc                                                       <  1:4935103/101‑1 (MQ=255)
                                          aGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAcc                                                       <  2:7096746/101‑1 (MQ=255)
                                             aaCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATg                                                    <  2:3106115/101‑1 (MQ=255)
                                             aaCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATg                                                    <  2:2764873/101‑1 (MQ=255)
                                             aaCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATg                                                    <  2:1184273/101‑1 (MQ=255)
                                                  gcCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGAGCACCATgtcgtc                                               <  1:6641253/101‑1 (MQ=255)
                                                     cGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTcgcg                                            <  1:3063280/101‑1 (MQ=255)
                                                      ggATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGa                                           >  2:7168459/1‑101 (MQ=255)
                                                        aTCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATg                                         <  2:2076211/101‑1 (MQ=255)
                                                        aTCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATg                                         >  1:3579549/1‑101 (MQ=255)
                                                          cAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGca                                       >  2:4729986/1‑101 (MQ=255)
                                                          cAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGca                                       >  2:5155698/1‑101 (MQ=255)
                                                           aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                      <  2:6635715/101‑1 (MQ=255)
                                                            ccgccgcCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcaca                                     <  2:4283866/100‑1 (MQ=255)
                                                              gccgccCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAg                                   <  2:2551408/101‑1 (MQ=255)
                                                               ccgcccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgc                                  <  2:851150/101‑1 (MQ=255)
                                                               ccgcccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTAGCGATGCACAAgc                                  >  2:7561790/1‑101 (MQ=255)
                                                                 gcccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgc                                >  2:7949699/1‑101 (MQ=255)
                                                                   ccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACaaagatcg                              >  2:8156250/1‑95 (MQ=255)
                                                                         gcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCgcggcgcggc                        >  1:8240160/1‑101 (MQ=255)
                                                                           aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGccc                      <  2:7797518/101‑1 (MQ=255)
                                                                           aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGccc                      <  2:5840441/101‑1 (MQ=255)
                                                                           aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGccc                      <  1:973174/101‑1 (MQ=255)
                                                                            aaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCg                     <  2:5493741/101‑1 (MQ=255)
                                                                            aaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCg                     <  2:8624230/101‑1 (MQ=255)
                                                                            aaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCg                     >  2:6463024/1‑101 (MQ=255)
                                                                             aCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCgg                    <  1:7076550/101‑1 (MQ=255)
                                                                               ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                  <  2:141525/101‑1 (MQ=255)
                                                                               ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                  <  2:619118/101‑1 (MQ=255)
                                                                               ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                  <  1:1414998/101‑1 (MQ=255)
                                                                                cGGGTGTTGATGCAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGaag                 >  1:8150584/1‑99 (MQ=255)
                                                                                cGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATa                 >  1:9003649/1‑101 (MQ=255)
                                                                                cGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATa                 <  2:6034794/101‑1 (MQ=255)
                                                                                  ggTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGc               >  2:8770914/1‑101 (MQ=255)
                                                                                    tgtTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGccc             <  2:1644852/101‑1 (MQ=255)
                                                                                    tgtTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGccc             <  1:764119/101‑1 (MQ=255)
                                                                                       tgatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc          <  1:7940225/101‑1 (MQ=255)
                                                                                       tgatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc          <  2:8296282/101‑1 (MQ=255)
                                                                                        gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagca         >  1:4419603/1‑101 (MQ=255)
                                                                                        gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagca         >  2:1314959/1‑101 (MQ=255)
                                                                                        gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGAGGCGCGGCCCGGATAGGCcagca         >  1:34383/1‑101 (MQ=255)
                                                                                          tgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCagcagc       >  2:6908386/1‑101 (MQ=255)
                                                                                           gaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCCgcg      >  1:41692/1‑101 (MQ=255)
                                                                                            aaaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAgcgc     >  2:4414148/1‑101 (MQ=255)
                                                                                             aaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGcc    >  2:3859718/1‑101 (MQ=255)
                                                                                              agccaaGGGGGGGCCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGcc    <  2:3691107/97‑1 (MQ=255)
                                                                                               ccAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTc  >  1:5770572/1‑101 (MQ=255)
                                                                                                    |                                                                                                
CCTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTC  >  NC_014034/2470153‑2470349

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 39 ≤  ATCG