Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,022 Δ1 bp 3.4% intergenic (+70/‑172) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0220C→. 3.4% 24.2 ‑0.3 ‑0.7326intergenic (+70/‑172)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/5):  ref base (154/160):  total (161/165)
Fisher's exact test strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.09e-01

Only 100 of 348 total aligned reads displayed.

GGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493923‑2494121
                                                                                                     |                                                                                                          
ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGGGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                            >  1:5295295/1‑101 (MQ=21)
ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                            >  1:4163004/1‑101 (MQ=21)
ggcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                            <  2:3732290/101‑1 (MQ=21)
   gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                         <  2:4257301/101‑1 (MQ=21)
    ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                        <  2:2465632/101‑1 (MQ=21)
    ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                        <  1:7834567/101‑1 (MQ=21)
    ggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                        <  2:92579/101‑1 (MQ=21)
      cgcgcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGCTATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                      <  1:6468131/101‑1 (MQ=21)
         gcgGCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                   <  2:1118283/101‑1 (MQ=21)
           ggCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                 <  2:5643495/101‑1 (MQ=21)
           ggCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                 <  1:4194395/101‑1 (MQ=21)
           ggCACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCCTTCATTTTg                                                                                                 >  1:5643702/1‑101 (MQ=18)
             cACGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                               <  2:5947047/101‑1 (MQ=21)
              aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGccc                                                                                              >  2:1895580/1‑101 (MQ=18)
              aCGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                              <  2:2770154/101‑1 (MQ=21)
               cGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                             <  1:6124724/101‑1 (MQ=21)
               cGACAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                             >  1:5141554/1‑101 (MQ=21)
                 aCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                           <  2:379302/101‑1 (MQ=21)
                 aCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                           <  1:8498056/101‑1 (MQ=21)
                 aCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                           <  1:3255690/101‑1 (MQ=21)
                 aCAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                           >  2:6218555/1‑101 (MQ=21)
                  cAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                          <  1:8146757/101‑1 (MQ=21)
                  cAGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTACCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                          <  2:5231288/101‑1 (MQ=21)
                  cAGGCCGAGTAA.GGGGGGGTCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                          <  1:3587970/101‑1 (MQ=21)
                   aGGCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCCCAAGCGGACTCCCCCCGCGATATTTTCAGGAAGATGAAACCGGATGCCTTTCATATTGCCCGACat                                                                                         >  2:8955022/1‑101 (MQ=18)
                    ggCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                        >  2:7981973/1‑101 (MQ=21)
                    ggCCGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                        <  2:6865874/101‑1 (MQ=21)
                      ccGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGCACTCCCCCCGGGATATTTGCAGCACGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                      <  1:6568343/101‑1 (MQ=21)
                       cgagtaggggggggg.CGCTGCCCCCCGTCCGCAAGCGGTCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                     <  2:4334386/93‑1 (MQ=18)
                       cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                     <  1:6324418/101‑1 (MQ=21)
                       cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                     <  2:812952/101‑1 (MQ=21)
                       cGAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCCAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                     >  1:1916425/1‑101 (MQ=18)
                        gAGTAAGGGGGGGG.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                    <  1:966386/101‑1 (MQ=18)
                           taagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                 >  2:7402294/5‑101 (MQ=255)
                           taagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                 <  1:1707389/97‑1 (MQ=255)
                            aagggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                <  1:3192936/98‑1 (MQ=255)
                            aagggggggc.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCcggg                                                                                <  1:8801286/98‑1 (MQ=18)
                             tcttccgatccgcTGCCCCCGGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCcggg                                                                                <  1:7232375/91‑1 (MQ=21)
                              gggggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAGGAAACCGGATGCCTTTCATCTTGCCCGAAATATCACggcggg                                                                              >  1:8301384/2‑101 (MQ=18)
                                 ggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                           <  2:6127768/101‑1 (MQ=255)
                                 ggggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                           <  1:1052772/101‑1 (MQ=255)
                                   ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                        <  1:99830/101‑1 (MQ=255)
                                   ggg.CGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                        <  2:7193470/101‑1 (MQ=255)
                                       cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                     >  1:734792/1‑101 (MQ=255)
                                           gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                              >  1:5765528/1‑99 (MQ=255)
                                           gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                              >  1:4600842/1‑99 (MQ=255)
                                            cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                            <  1:9107171/101‑5 (MQ=255)
                                               cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                          <  2:3582138/97‑7 (MQ=255)
                                                ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                         <  2:2574335/98‑8 (MQ=255)
                                                 cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGCATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                        <  1:8955443/99‑9 (MQ=21)
                                                            ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                             >  2:7463652/1‑97 (MQ=18)
                                                             gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGGcgcgcc                                             >  2:5435998/1‑96 (MQ=18)
                                                             gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                         >  2:3447109/1‑101 (MQ=18)
                                                             gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGCG.c.c                                         >  2:264577/1‑101 (MQ=18)
                                                              aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cc                                        >  1:489283/1‑101 (MQ=18)
                                                               cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.c.ccc                                       <  2:882595/101‑1 (MQ=17)
                                                                 ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccc                                     >  2:5039857/1‑100 (MQ=18)
                                                                   ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccccg                                   >  1:6917471/1‑98 (MQ=17)
                                                                    cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccctgc                                  <  2:7704352/101‑5 (MQ=17)
                                                                      cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGccc                                 >  2:4432444/1‑101 (MQ=17)
                                                                      cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGccc                                 <  2:1626352/101‑1 (MQ=17)
                                                                       gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGcccc                                >  1:4013546/1‑101 (MQ=0)
                                                                       gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGcccc                                <  2:2158618/101‑1 (MQ=17)
                                                                        ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcg                               >  1:2146677/1‑101 (MQ=2)
                                                                        ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcg                               >  1:1831706/1‑101 (MQ=2)
                                                                            atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcgccgg                           <  1:7555424/101‑1 (MQ=2)
                                                                            atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcgg                           >  2:3274308/1‑101 (MQ=18)
                                                                             tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGt                          >  2:3144781/1‑101 (MQ=18)
                                                                               tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.CGCGCC.CCCCTGCCCcggcggccg                        >  2:3906303/1‑98 (MQ=17)
                                                                               tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                        <  2:6445306/101‑1 (MQ=17)
                                                                                gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                       >  2:664137/1‑101 (MQ=17)
                                                                                gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                       <  1:700798/101‑1 (MQ=17)
                                                                                gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                       >  2:4482595/1‑101 (MQ=18)
                                                                                gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGGCCg                       >  2:6821784/1‑101 (MQ=17)
                                                                                 cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGt                      <  1:3359253/101‑1 (MQ=17)
                                                                                    caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggc                   >  1:132532/1‑101 (MQ=18)
                                                                                    caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggc                   >  1:2412558/1‑101 (MQ=18)
                                                                                     aataTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcg                  >  1:2602822/4‑101 (MQ=17)
                                                                                      aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcgg                 >  1:6193330/1‑101 (MQ=17)
                                                                                      aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcgg                 >  2:3636282/1‑101 (MQ=18)
                                                                                       gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcggc                >  2:7092140/1‑101 (MQ=18)
                                                                                          gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCTC.C.CCCCTGCCCCGGCGGTACGTGgccgcggc             >  2:1494372/1‑99 (MQ=14)
                                                                                          gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc             >  2:1991524/1‑101 (MQ=18)
                                                                                           aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt            <  2:1258087/101‑1 (MQ=17)
                                                                                           aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt            <  2:2828971/101‑1 (MQ=17)
                                                                                           aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGCAGCCC.C.CCCCTGCCCCGCCGGCCGGTGGGGGCGCCt            >  1:1127422/1‑101 (MQ=12)
                                                                                           aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCGGCCCCGGCGGGCCGTGGCGGCGCCt            >  1:1184577/1‑101 (MQ=14)
                                                                                           aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CGCCCCTGCCCCGCCGGTCCGCGGGGGCCccc            >  1:290759/1‑100 (MQ=9)
                                                                                            aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt           <  2:5378781/101‑1 (MQ=17)
                                                                                            aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt           >  2:766081/1‑101 (MQ=18)
                                                                                             aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg          <  1:7982313/101‑1 (MQ=17)
                                                                                              ccGGATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa         <  1:382543/101‑1 (MQ=17)
                                                                                               cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        >  2:2872290/1‑101 (MQ=18)
                                                                                               cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGAC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        >  2:4739024/1‑101 (MQ=18)
                                                                                                 gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CGAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg      >  1:7691488/1‑101 (MQ=18)
                                                                                                 gATGCCTTTCATCTGACCCGAAATATCCCGGGGGGTGAA.CGGCA.A.AG.CCAGAGGGGGGCAACGC.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg      <  2:4675163/101‑1 (MQ=18)
                                                                                                  aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg     >  2:1057911/1‑101 (MQ=18)
                                                                                                  aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg     <  2:4910541/101‑1 (MQ=17)
                                                                                                  aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg     >  1:8056151/1‑101 (MQ=17)
                                                                                                   tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGGA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc    <  2:847206/101‑1 (MQ=17)
                                                                                                     |                                                                                                          
GGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGG.CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493923‑2494121

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG