BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,047	Δ1 bp	4.3%	intergenic (+95/‑147)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,047	0	G→.	4.3%	27.3 ‑0.6 ‑0.2	279	intergenic (+95/‑147)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (6/6): ref base (156/111): total (162/117)
Fisher's exact test strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.13e-01

Only 100 of 318 total aligned reads displayed.


AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146


 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                          >  2:7402294/5‑101 (MQ=255)


 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                          <  1:1707389/97‑1 (MQ=255)


 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                          >  2:3951065/5‑101 (MQ=255)


  aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                         <  1:3192936/98‑1 (MQ=255)


    ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                                       <  2:7701644/100‑1 (MQ=255)


    ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                                       <  2:6346311/100‑1 (MQ=255)


     gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                                                      >  2:4399036/1‑100 (MQ=255)


      ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                     <  2:1946823/101‑1 (MQ=255)


      ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                     <  1:3931415/101‑1 (MQ=255)


       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                    <  1:1052772/101‑1 (MQ=255)


       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGa                                                                                                    <  1:8155471/101‑1 (MQ=255)


       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCATTTCATCTTGCCCGAAATATCCCGGGGGGCGa                                                                                                    >  1:121102/1‑101 (MQ=17)


          ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                <  1:7297136/101‑1 (MQ=255)


            cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                              <  1:3525941/101‑1 (MQ=255)


            cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                              >  1:4154401/1‑101 (MQ=255)


             gcTGCCCCCCGTCCGCAAGCGGACTCCCCGCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                           <  2:2362552/101‑1 (MQ=255)


             gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                           <  1:3322414/101‑1 (MQ=255)


                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                       >  1:4600842/1‑99 (MQ=255)


                 cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                      >  2:8117116/1‑98 (MQ=255)


                  ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                    >  2:5877657/1‑96 (MQ=255)


                      cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGCATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                 <  1:8955443/99‑9 (MQ=21)


                             aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCa                                                                         >  2:2139550/1‑101 (MQ=18)


                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.ccc                                                                 >  1:489283/1‑101 (MQ=18)


                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.ccc                                                                 >  2:8319079/1‑101 (MQ=18)


                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCG.cccc                                                                <  2:882595/101‑1 (MQ=17)


                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCG.cccc                                                                <  1:7990667/101‑1 (MQ=17)


                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccc                                                                >  1:2429977/1‑101 (MQ=18)


                                      ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccc                                                              >  2:5039857/1‑100 (MQ=18)


                                        ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccctg                                                            <  1:8368439/101‑4 (MQ=17)


                                         cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cccccctgc                                                           <  1:560356/101‑5 (MQ=17)


                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGccc                                                          >  2:4434598/1‑101 (MQ=18)


                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.ccccactgctc                                                         >  2:7215451/1‑94 (MQ=17)


                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGcccc                                                         >  2:1083124/1‑101 (MQ=18)


                                              gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                       <  2:2808375/101‑2 (MQ=0)


                                              gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                       >  2:8980044/1‑101 (MQ=18)


                                              gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                       <  2:314687/101‑1 (MQ=17)


                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg                                                     >  2:4504676/1‑101 (MQ=18)


                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg                                                     >  1:6499480/1‑101 (MQ=18)


                                                 atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccgg                                                    <  2:3069142/101‑1 (MQ=2)


                                                 atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccgg                                                    <  1:7555424/101‑1 (MQ=2)


                                                 atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                    >  2:3274308/1‑101 (MQ=18)


                                                 atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                    >  1:9097314/1‑101 (MQ=17)


                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                 <  2:6445306/101‑1 (MQ=17)


                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                >  2:5384668/1‑101 (MQ=18)


                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCg                                                >  2:6821784/1‑101 (MQ=17)


                                                     gcagcaAGATGAAACCGGATGCCTTTCACCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                <  1:6395668/101‑1 (MQ=17)


                                                      cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt                                               <  1:1876870/101‑1 (MQ=17)


                                                         caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc                                            <  1:7074252/101‑1 (MQ=17)


                                                         caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc                                            >  1:132532/1‑101 (MQ=18)


                                                          aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TTGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                           >  1:996582/1‑101 (MQ=18)


                                                           aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                          >  2:257776/1‑101 (MQ=18)


                                                            gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcggc                                         >  2:7092140/1‑101 (MQ=18)


                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCAGCTC.CCCCCTGCCCCGGCGGTACGTGgccgcggc                                      >  2:1494372/1‑99 (MQ=14)


                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                      <  2:5453497/101‑1 (MQ=17)


                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                      >  2:1991524/1‑101 (MQ=18)


                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                      >  2:6677205/1‑101 (MQ=18)


                                                                aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                     >  2:3606184/1‑101 (MQ=18)


                                                                aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCAGCCC.CCCCCTGCCCCGCCGGCCGGTGGGGGCGCCt                                     >  1:1127422/1‑101 (MQ=12)


                                                                  aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                   <  2:2648292/101‑1 (MQ=17)


                                                                  aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                   <  2:3848032/101‑1 (MQ=17)


                                                                   ccGGATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                  <  1:382543/101‑1 (MQ=17)


                                                                   ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                  <  2:7773691/101‑1 (MQ=17)


                                                                   ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                  <  1:7402618/101‑1 (MQ=17)


                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                 <  2:3641976/101‑1 (MQ=17)


                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGACCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                 >  2:4739024/1‑101 (MQ=18)


                                                                     ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                >  2:5337349/1‑101 (MQ=18)


                                                                      gATGCCTTTCATCTGACCCGAAATATCCCGGGGGGTGAA.CGGCA.A.A.G.CCAGAGGGGGGCAACGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                               <  2:4675163/101‑1 (MQ=18)


                                                                       aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                              >  1:8056151/1‑101 (MQ=17)


                                                                        tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                             >  1:7193553/1‑101 (MQ=18)


                                                                        cgCCTCTAATCTTGCCCGAAATATCCCGGGGGCTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtttgtcgag                             <  2:602038/100‑8 (MQ=9)


                                                                         gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                            <  1:574201/101‑1 (MQ=17)


                                                                         gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                            >  2:7471263/1‑101 (MQ=18)


                                                                         gCCTTTCATCTTGCCCGAAATATCCCGGGGGATGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                            >  2:4274964/1‑101 (MQ=18)


                                                                            tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         <  1:7765654/101‑1 (MQ=17)


                                                                            tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         <  2:4974623/101‑1 (MQ=17)


                                                                              tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                       <  2:3424366/101‑1 (MQ=18)


                                                                                 tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                    >  1:5222058/1‑101 (MQ=18)


                                                                                  cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                   >  1:5063574/1‑101 (MQ=18)


                                                                                  cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCCGGGGGGCgg                   >  1:6718963/1‑101 (MQ=17)


                                                                                   ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                  <  2:3767920/101‑1 (MQ=18)


                                                                                   ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                  >  2:1775279/1‑101 (MQ=18)


                                                                                   tggCCCGAAATTCCCGGGGGGGTGAT.TGGC..G.ACG.CCGGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                  <  1:4387371/99‑1 (MQ=21)


                                                                                    tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                 >  1:6116736/1‑101 (MQ=18)


                                                                                    tGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCCTGACGGGCGGGGGGCCGGGc                 >  2:2903674/1‑101 (MQ=12)


                                                                                     gCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                >  1:8714205/1‑101 (MQ=18)


                                                                                      cccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt               <  1:3049734/101‑1 (MQ=18)


                                                                                       ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt              <  2:1145603/101‑1 (MQ=18)


                                                                                        cGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa             <  1:2693132/101‑1 (MQ=18)


                                                                                         gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa            <  2:9132726/101‑1 (MQ=18)


                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaaac          >  1:8235956/1‑99 (MQ=18)


                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc          >  1:767916/1‑101 (MQ=21)


                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc          >  2:1240412/1‑101 (MQ=21)


                                                                                            atatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg         >  2:8501128/1‑101 (MQ=21)


                                                                                            atagCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg         <  2:489275/101‑1 (MQ=21)


                                                                                              atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa       <  1:6218794/101‑1 (MQ=21)


                                                                                              atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa       >  1:2297369/1‑101 (MQ=21)


                                                                                              atCCCGGGGGGTGAA.GGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa       <  2:1352591/101‑1 (MQ=21)


                                                                                                 ccGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt    <  2:5608592/101‑1 (MQ=21)


                                                                                                 ccGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt    <  2:4151863/101‑1 (MQ=21)


                                                                                                   ggggggCGAA.TGGC..GAA.G.CCAGAGGGGGGCACCGC.CCCCCTGCCCCGGCGGCCCGTGGGGGCGCCTTGACGGGAGGGGGCGCGGGCACTAACCGAAATTga  >  1:9198254/1‑100 (MQ=19)


AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG