Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,525,812 Δ1 bp 99.0% coding (1405/1494 nt) RCAP_rcc02356 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,525,8120C→.99.0% 2.2 ‑2.9 ‑1.9401coding (1405/1494 nt)RCAP_rcc02356hypothetical protein
Reads supporting (aligned to +/- strand):  new base (205/192):  ref base (0/4):  total (205/196)
Fisher's exact test strand distribution p-value = 5.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.31e-02

Only 100 of 456 total aligned reads displayed.

AAGGAGCGTATTTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTTCCCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTCGGGCCAGGCC  >  NC_014034/2525712‑2525909
                                                                                                    |                                                                                                 
 aGGAGCGTATTTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTTcc                                                                                                  <  2:4065597/101‑1 (MQ=255)
 aGGAGCGTATTTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTTcc                                                                                                  >  1:5379833/1‑101 (MQ=255)
  ggAGCGTATTTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTTccc                                                                                                 <  1:867385/101‑1 (MQ=255)
   gAGCGTATGTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTTcccc                                                                                                <  2:8398309/101‑1 (MQ=255)
       gTATTTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTTccccgccc                                                                                            >  1:8143625/1‑97 (MQ=255)
        tATTTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.ccccgcccc                                                                                          <  1:7027992/101‑1 (MQ=255)
         aTTTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.ccccgccccg                                                                                         >  2:817421/1‑101 (MQ=255)
            tAGGCCAAGAAGAACCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATc                                                                                      <  1:5861834/101‑1 (MQ=255)
             aGGCCAAGAAGAACCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCa                                                                                     <  2:4709649/101‑1 (MQ=255)
                ccAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAgcg                                                                                  >  1:7120040/1‑101 (MQ=255)
                 cAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAgcgc                                                                                 <  1:6236635/101‑1 (MQ=255)
                 cAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAgcgc                                                                                 >  2:549213/1‑101 (MQ=255)
                  aagaagAACCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGcc                                                                                <  2:7978640/101‑1 (MQ=255)
                   agaagaAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCg                                                                               >  1:4941555/1‑101 (MQ=255)
                     aagaaACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGaa                                                                             >  2:2423465/1‑101 (MQ=255)
                     aagaaACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGaa                                                                             >  1:3356575/1‑101 (MQ=255)
                      agaaCCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGaaa                                                                            <  2:8648884/101‑1 (MQ=255)
                      agaaACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGaaa                                                                            >  2:1475174/1‑101 (MQ=255)
                      agaaACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGaaa                                                                            <  1:6482220/101‑1 (MQ=255)
                      agaaACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGaaa                                                                            <  1:1723009/101‑1 (MQ=255)
                      agaaACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATGTGGCCTT.CCCCGCCCCGATCAGCGCCGaaa                                                                            >  1:3759940/1‑101 (MQ=255)
                       gaaACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAg                                                                           <  2:6934857/101‑1 (MQ=255)
                        aacccccTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAgc                                                                          <  1:3717582/98‑1 (MQ=255)
                        aaaCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAgc                                                                          >  2:5674677/1‑101 (MQ=255)
                        aaaCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAgc                                                                          >  2:3316942/1‑101 (MQ=255)
                        aaaCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAgc                                                                          >  2:740241/1‑101 (MQ=255)
                         aaCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAgcg                                                                         >  1:1082193/1‑101 (MQ=255)
                         aaCCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAgcg                                                                         >  1:6955329/1‑101 (MQ=255)
                                gCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAg                                                                  <  2:5007336/101‑1 (MQ=255)
                                  cGTTTCTTCTTGGGGAAAATACGCTTTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAggc                                                                >  1:7489418/1‑100 (MQ=255)
                                  cGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGt                                                                >  2:617733/1‑101 (MQ=255)
                                   gTTTCTTCTTGGGGAAAATGCGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTCGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGtt                                                               <  1:7163031/101‑1 (MQ=255)
                                   gTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGtt                                                               >  2:76432/1‑101 (MQ=255)
                                    tttCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTg                                                              >  1:2992428/1‑101 (MQ=255)
                                     ttcttcTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGCAAGCGGcgcggcgctgc                                                             >  1:2890959/1‑94 (MQ=255)
                                     ttcttcTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGt                                                             >  2:8869855/1‑101 (MQ=255)
                                     ttcttcTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGt                                                             >  1:7298938/1‑101 (MQ=255)
                                       cttcttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTAc                                                           >  2:3950673/1‑101 (MQ=255)
                                       cttcttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTAc                                                           >  1:2473270/1‑101 (MQ=255)
                                        ttcttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACt                                                          >  1:2274764/1‑101 (MQ=255)
                                        ttcttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACt                                                          >  2:1294310/1‑101 (MQ=255)
                                         tcttGGGGAAAATCCGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACtt                                                         <  1:6132464/101‑1 (MQ=255)
                                         tcttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACtt                                                         >  2:6833353/1‑101 (MQ=255)
                                         tcttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACtt                                                         <  1:5226006/101‑1 (MQ=255)
                                          cttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGCAAGCGGCGCGAGGTTGTACTTc                                                        >  1:1683590/1‑101 (MQ=255)
                                          cttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTc                                                        >  2:9072157/1‑101 (MQ=255)
                                          cttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTc                                                        >  1:4023582/1‑101 (MQ=255)
                                          cttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTc                                                        >  1:199574/1‑101 (MQ=255)
                                          cttGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTc                                                        <  1:2124933/101‑1 (MQ=255)
                                              gggAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGccc                                                    <  2:7733950/101‑1 (MQ=255)
                                                 aaaaTACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCcct                                                 <  2:2563364/101‑1 (MQ=255)
                                                   aaTACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCcctcc                                               >  2:4866336/1‑101 (MQ=255)
                                                   aaTACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCcctcc                                               <  1:5596862/101‑1 (MQ=255)
                                                   aaTACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCcctcc                                               >  1:7620741/1‑101 (MQ=255)
                                                    aTACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCcctcct                                              >  1:3286484/1‑101 (MQ=255)
                                                    aTACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCcctcct                                              >  1:3566969/1‑101 (MQ=255)
                                                    aTACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCcctcct                                              <  1:4355923/101‑1 (MQ=255)
                                                    aTACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCcctcct                                              >  1:8385497/1‑101 (MQ=255)
                                                     tACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCctcctc                                             <  1:3049058/101‑1 (MQ=255)
                                                     tACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCctcctc                                             <  2:3871408/101‑1 (MQ=255)
                                                      aCGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCctct                                            <  2:8784993/101‑1 (MQ=255)
                                                      aCGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCctct                                            >  1:1645297/1‑101 (MQ=255)
                                                      aCGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCctct                                            >  1:723913/1‑101 (MQ=255)
                                                      aCGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCctct                                            >  2:7128875/1‑101 (MQ=255)
                                                       cGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTg                                           <  2:1967773/101‑1 (MQ=255)
                                                        gCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGGGAGGTTGTACTTCGCCCCCTCCTCTGc                                          >  1:8912723/1‑101 (MQ=255)
                                                        gCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGc                                          <  2:1829941/101‑1 (MQ=255)
                                                          tctTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCCT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCgg                                        >  2:3141428/1‑101 (MQ=255)
                                                           ctTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGc                                       >  1:1269703/1‑101 (MQ=255)
                                                             tGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGccc                                     >  2:7850107/1‑101 (MQ=255)
                                                              gccgggggccggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCa                                    <  2:5959840/101‑1 (MQ=255)
                                                               ccgggggccggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAg                                   >  2:2182176/1‑101 (MQ=255)
                                                                cgggggccggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAgg                                  <  2:2909113/101‑1 (MQ=255)
                                                                cgggggccggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAgg                                  >  2:3620715/1‑101 (MQ=255)
                                                                 gggggccggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGc                                 <  2:1146525/101‑1 (MQ=255)
                                                                  ggggccggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCg                                >  1:6647326/1‑101 (MQ=255)
                                                                     gccggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTc                             <  1:4144838/101‑1 (MQ=255)
                                                                      ccggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCa                            <  2:8978018/101‑1 (MQ=255)
                                                                       cggggCGGGCGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAg                           >  2:1525700/1‑101 (MQ=255)
                                                                         gggcgggcGCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGAc                         >  1:8260212/1‑101 (MQ=255)
                                                                          ggcgggcgCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGAcc                        <  1:2751387/101‑1 (MQ=255)
                                                                          ggcgggcgCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGAcc                        <  2:3727538/101‑1 (MQ=255)
                                                                           gcgggcgCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCg                       <  2:6715612/101‑1 (MQ=255)
                                                                           gcgggcgCGGCGCCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCg                       <  1:7834941/101‑1 (MQ=255)
                                                                                cgcggcgcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGAc                  <  1:506213/101‑1 (MQ=255)
                                                                                 gcggcgcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACg                 <  1:9072579/101‑1 (MQ=255)
                                                                                  cggcgcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACgg                >  1:5720500/1‑101 (MQ=255)
                                                                                   ggcgcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGa               >  2:3487600/1‑101 (MQ=255)
                                                                                   ggcgcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGa               >  2:6393105/1‑101 (MQ=255)
                                                                                    gcgcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGAt              >  2:7457140/1‑101 (MQ=255)
                                                                                     cgcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGAtt             <  2:5204044/101‑1 (MQ=255)
                                                                                     cgcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGAtt             <  1:5994156/101‑1 (MQ=255)
                                                                                      gcCTATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTc            <  2:8470772/101‑1 (MQ=255)
                                                                                         tATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTCggg         >  1:4724030/1‑101 (MQ=255)
                                                                                         tATTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCCGCCCAGGCGGTCAGACCGGCGACGGATACggg         >  2:4340623/1‑101 (MQ=255)
                                                                                          aTTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTCGGGc        >  2:7788733/1‑101 (MQ=255)
                                                                                          aTTTGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTCGGGc        >  1:8018273/1‑101 (MQ=255)
                                                                                            ttGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTCGGGCCa      <  2:7615183/101‑1 (MQ=255)
                                                                                            ttGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTCGGGCCa      <  2:723903/101‑1 (MQ=255)
                                                                                            ttGGCCTT.CCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTCGGGCCa      >  2:4384896/1‑101 (MQ=255)
                                                                                                    |                                                                                                 
AAGGAGCGTATTTAGGCCAAGAAGAAACCCCTGCCGTTTCTTCTTGGGGAAAATACGCTCTTGCCGGGGGCCGGGGCGGGCGCGGCGCCTATTTGGCCTTCCCCCGCCCCGATCAGCGCCGAAAGCGGCGCGAGGTTGTACTTCGCCCCCTCCTCTGCGGCCCAGGCGGTCAGACCGGCGACGGATTCGGGCCAGGCC  >  NC_014034/2525712‑2525909

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG