Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,539,113 Δ1 bp 1.9% intergenic (‑106/+9) RCAP_rcc02366 ← / ← RCAP_rcc02367 NnrS family protein/calcium‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,539,1130G→. 1.9% 9.2 ‑2.2 ‑0.0314intergenic (‑106/+9)RCAP_rcc02366/RCAP_rcc02367NnrS family protein/calcium‑binding protein
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (147/160):  total (153/161)
Fisher's exact test strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.76e-01

Only 100 of 368 total aligned reads displayed.

CTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCCGCGA  >  NC_014034/2539013‑2539213
                                                                                                     |                                                                                                    
cTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.g                                                                                                      <  1:5758000/101‑1 (MQ=255)
cTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.g                                                                                                      <  1:8271812/101‑1 (MQ=255)
cTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.g                                                                                                      <  1:8919063/101‑1 (MQ=255)
cTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.g                                                                                                      <  1:7661288/101‑1 (MQ=255)
  ccGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.ggg                                                                                                    >  2:8384022/1‑101 (MQ=255)
    ggCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.ggggg                                                                                                  >  2:7888927/1‑101 (MQ=255)
    ggCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.ggggg                                                                                                  <  2:142242/101‑1 (MQ=255)
     gCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.gggggg                                                                                                 <  1:3156979/101‑1 (MQ=255)
       aGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.gggggggg                                                                                               >  1:4092924/1‑101 (MQ=255)
         cGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGt                                                                                             >  2:4636597/1‑101 (MQ=255)
          ggggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTc                                                                                            <  2:6393414/101‑1 (MQ=255)
          ggggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTc                                                                                            <  2:2766723/101‑1 (MQ=255)
          ggggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTc                                                                                            <  2:5416033/101‑1 (MQ=255)
           gggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCa                                                                                           <  2:7674550/101‑1 (MQ=255)
            ggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAg                                                                                          >  2:2385149/1‑101 (MQ=255)
              gtGTCCGGGGGGTGGCCCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGcc                                                                                        <  2:2115683/100‑1 (MQ=255)
              aTGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGcc                                                                                        >  2:5543250/1‑101 (MQ=255)
              aTGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGGCAGcc                                                                                        >  1:4478460/1‑101 (MQ=255)
               tGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGTGGCGCCGTGCC.GGGGGGGGGTCAGCcg                                                                                       >  2:755890/1‑101 (MQ=255)
                   cGGGTGGTGGCGCCCGGCC.CCGGGCCGGCAAAGGGGGGGAGCGGCAAAAACCCGGGGGCGGGGGCTTGCGGGCGCGGGCC.GGGGGGGGGGCGGcccggggg                                                                                  >  2:4222410/1‑95 (MQ=255)
                   cGGGGGGTGGCGCCCGGCCGCCGGGCTGTCAAGGGGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCgcggc                                                                                   <  2:1936570/101‑1 (MQ=255)
                    gggTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCgcggcg                                                                                  <  1:294551/101‑1 (MQ=255)
                     ggtggtGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC..GGGGGGGGTCAGCCgcggcggc                                                                                >  2:4801058/1‑101 (MQ=255)
                      gtggtgGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCgcggcggc                                                                                <  2:3801168/101‑1 (MQ=255)
                       tggtggCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCgcggcggcg                                                                               >  1:7302133/1‑101 (MQ=255)
                       gggtggCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCgcggcggcg                                                                               <  2:6192055/100‑1 (MQ=255)
                        ggtggCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGgcgc                                                                              >  1:6998931/1‑101 (MQ=255)
                         gtggCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGcgcg                                                                             >  1:6150484/1‑101 (MQ=255)
                            gcgcCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTc                                                                          <  1:5125285/101‑1 (MQ=255)
                             cgcCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTcc                                                                         <  1:6677318/101‑1 (MQ=255)
                              gcCCGGCCGCCGGGCTGTCAAGGGGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCg                                                                        <  1:8540102/101‑1 (MQ=255)
                                ccGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGaa                                                                      >  2:8019039/1‑101 (MQ=255)
                                 cGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGaaa                                                                     <  2:6830845/101‑1 (MQ=255)
                                 cGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGAGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGaaa                                                                     >  2:4442848/1‑101 (MQ=255)
                                  ggCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAAt                                                                    >  1:1986676/1‑101 (MQ=255)
                                   gccgccGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCAAAATc                                                                   <  2:4038937/101‑1 (MQ=255)
                                      gccgGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGc                                                                <  1:5932161/101‑1 (MQ=255)
                                       ccgGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGcc                                                               >  1:2791972/1‑101 (MQ=255)
                                        cgGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCa                                                              >  2:2504585/1‑101 (MQ=255)
                                        cgGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCa                                                              <  2:6739324/101‑1 (MQ=255)
                                          ggCTGACAGGGTGGGGGTCCGCCAAAGCCCCGGTGGATGGGCTTTGCGTCGCCGTGCC.GGGGGGGTTTCATCCGCGGCGGCGCGGTCCGAAATCGGCCAGc                                                            <  2:4342951/101‑1 (MQ=255)
                                           gCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGGGCGGTCCGGAATCGGCCAGCg                                                           >  2:5241346/1‑101 (MQ=255)
                                            cTGTCAAGGTGGGGATGCGCCAAACCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGt                                                          <  2:3060449/101‑1 (MQ=255)
                                              gTCAGGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGTCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTca                                                        <  2:5082548/101‑1 (MQ=255)
                                              gTCAAGGTGGGGATGCGCCAAACCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTca                                                        <  2:726570/101‑1 (MQ=255)
                                              gTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTcc                                                        >  2:2474007/1‑100 (MQ=255)
                                               tCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGGCCGAAATCGGCCAGCGGcac                                                       >  2:257664/1‑101 (MQ=255)
                                                ccaaggggggACGCGCCAAAACCCCGGTGCAAGGGGGTTGCCGCGCCGGGCC.GGGGGGGGGCCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTcacc                                                      <  2:7354471/95‑1 (MQ=255)
                                                cAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTcacc                                                      <  1:2483828/101‑1 (MQ=255)
                                                cAAGGTGGGGAGCCGCCAAACCCCCGGTGCGGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTcacc                                                      <  2:8906777/101‑1 (MQ=255)
                                                  aGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTcaccac                                                    >  2:6349184/1‑101 (MQ=255)
                                                  aGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTcaccac                                                    <  2:6299377/101‑1 (MQ=255)
                                                  aGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTcaccac                                                    <  2:7422837/101‑1 (MQ=255)
                                                  aGGTGGGGAGGCGCCAAAACCCCGGGGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTcaccac                                                    <  1:5584186/101‑1 (MQ=255)
                                                      ggggATGCGCCACAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGGCAGCCGCGGCGGCGCGGGCCGAAATCGGCCAGCGCCCCCACGCCg                                                >  1:8179084/1‑101 (MQ=255)
                                                      ggggATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCg                                                >  1:3581688/1‑101 (MQ=255)
                                                      ggggATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCg                                                >  1:4770746/1‑101 (MQ=255)
                                                       gggATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGt                                               >  1:5426244/1‑101 (MQ=255)
                                                        ggATGCGCCAAACCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTc                                              <  2:3381578/101‑1 (MQ=255)
                                                        ggATGCGCCAAACCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTc                                              <  2:7064921/101‑1 (MQ=255)
                                                          aTGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGc                                            >  2:3609784/1‑101 (MQ=255)
                                                          aTGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGc                                            >  1:4185776/1‑101 (MQ=255)
                                                           tGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGcc                                           >  2:1164150/1‑101 (MQ=255)
                                                            gcgcCAAACCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCg                                          <  1:2454508/101‑1 (MQ=255)
                                                            gcgcCAAAACCCGGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCg                                          <  2:4478325/101‑1 (MQ=255)
                                                            gcgcCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCg                                          <  1:8384385/101‑1 (MQ=255)
                                                               ccAAACCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTg                                       <  2:3504059/101‑1 (MQ=255)
                                                                cAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGc                                      >  1:1195633/1‑101 (MQ=255)
                                                                cAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGc                                      <  1:7970569/101‑1 (MQ=255)
                                                                cAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGc                                      >  1:5304220/1‑101 (MQ=255)
                                                                 aaacCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCg                                     <  2:5475807/101‑1 (MQ=255)
                                                                   acccccGTTGCCGGGGCCTTGCGGCGCCGTGCC.GGGGGGGCCTCAGCCGCGGCGCCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGt                                   <  2:8146230/99‑1 (MQ=255)
                                                                   aaCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGt                                   >  2:8621357/1‑101 (MQ=255)
                                                                    aCCCGGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTc                                  <  1:654961/101‑1 (MQ=255)
                                                                       ccGGTGAAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGAGGGCGGAAATCGGCCAGCGTCACAAAGCCGGGGCCGGGGGGGTcgcg                               >  2:2929111/1‑101 (MQ=255)
                                                                        cGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCGGCGTCACCACGCCGTCGCCGGTGCGGTCgcgc                              >  1:4672894/1‑101 (MQ=255)
                                                                          gggCAGGGGGCTGGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCAt                            <  1:2309667/99‑1 (MQ=255)
                                                                          gTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCAt                            >  2:5732228/1‑101 (MQ=255)
                                                                          gTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCAt                            >  2:4274846/1‑101 (MQ=255)
                                                                            gCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATcc                          >  1:4284583/1‑100 (MQ=255)
                                                                             cAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGc                         >  1:3284195/1‑101 (MQ=255)
                                                                             cAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGc                         <  2:1622948/101‑1 (MQ=255)
                                                                              aGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCa                        >  2:132507/1‑101 (MQ=255)
                                                                               gggggCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCaa                       >  1:1013216/1‑101 (MQ=255)
                                                                                 gggCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAAc                     <  1:7173917/101‑1 (MQ=255)
                                                                                  ggCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAAcc                    <  1:6326111/101‑1 (MQ=255)
                                                                                   gCTTGCGGCGCCGTGCCGGGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGCAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGGCGCGCATCGCAACcc                    >  2:9104801/1‑101 (MQ=255)
                                                                                   gCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCa                   <  2:597082/101‑1 (MQ=255)
                                                                                   gCTTGCGGCGCCGGGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCa                   <  2:2047674/101‑1 (MQ=255)
                                                                                     ttGCGGCGCCGGGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGc                 <  1:494085/101‑1 (MQ=255)
                                                                                        gggcgCCGGGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGAt              <  1:2361122/100‑1 (MQ=255)
                                                                                         ggcgCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATc             >  1:5506292/1‑101 (MQ=255)
                                                                                            gcCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCt          >  2:2330/1‑101 (MQ=255)
                                                                                             ccGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTg         <  2:2610644/101‑1 (MQ=255)
                                                                                              cGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGc        <  1:6215894/101‑1 (MQ=255)
                                                                                              cGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGc        >  1:3952371/1‑101 (MQ=255)
                                                                                               gTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGcc       <  1:7560091/101‑1 (MQ=255)
                                                                                                 gccggggggggggTCAGCCGCAGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGccc      <  1:9105223/97‑1 (MQ=255)
                                                                                                 gCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCcg     <  2:7823426/101‑1 (MQ=255)
                                                                                                 gCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTAACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCcg     <  1:153021/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGGTCAGCCGCGGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCCGCGA  >  NC_014034/2539013‑2539213

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG