BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,559,935	Δ1 bp	100%	intergenic (‑211/+59)	ispZ ← / ← ftsY	intracellular septation protein A/cell division protein FtsY

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,559,935	0	C→.	100.0%	70.4	285	intergenic (‑211/+59)	ispZ/ftsY	intracellular septation protein A/cell division protein FtsY
Reads supporting (aligned to +/- strand): new base (182/77): ref base (0/26): total (182/103)
Fisher's exact test strand distribution p-value = 3.30e-13
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.27e-12

Only 100 of 390 total aligned reads displayed.


CGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGCGCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTCCTC  >  NC_014034/2559835‑2560035


cGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAgcgc                                                                                                      >  1:838189/1‑101 (MQ=255)


 ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGcc                                                                                                     >  2:3200101/1‑101 (MQ=255)


 ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGcc                                                                                                     >  1:1141738/1‑101 (MQ=255)


  gTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccc                                                                                                    <  1:10094/101‑1 (MQ=255)


   tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                                   >  1:1877669/1‑101 (MQ=255)


    ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccc                                                                                                  >  2:8691682/1‑101 (MQ=255)


    ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccc                                                                                                  >  1:7835684/1‑101 (MQ=255)


    ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccc                                                                                                  >  2:488367/1‑101 (MQ=255)


     tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGcccccc                                                                                                 <  2:5026859/101‑1 (MQ=255)


      ccTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccg                                                                                                >  1:4887148/1‑100 (MQ=255)


      ccTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccg                                                                                                >  1:5574572/1‑100 (MQ=255)


       cTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccga                                                                                               >  2:552084/1‑99 (MQ=255)


        tGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGGGccccccgag                                                                                              >  2:4253618/1‑98 (MQ=255)


        tGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccgaa                                                                                              >  2:6442185/1‑98 (MQ=255)


         gACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccgaag                                                                                             >  1:9051100/1‑97 (MQ=255)


         gACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccgaag                                                                                             >  1:2453891/1‑97 (MQ=255)


         gACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccgaag                                                                                             <  1:1575995/101‑5 (MQ=255)


          aCAGGATTGTGCCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccgnncc                                                                                            >  1:5741780/1‑96 (MQ=255)


            aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAgcgc                                                                                         >  1:7127176/1‑101 (MQ=255)


            aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAgcgc                                                                                         >  2:7686601/1‑101 (MQ=255)


             ggATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGcc                                                                                        >  1:3099383/1‑101 (MQ=255)


              gATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGACGCGCCt                                                                                       >  1:2982702/1‑101 (MQ=255)


               aTTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTg                                                                                      >  1:8519421/1‑101 (MQ=255)


                ttGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGCAGCGCCTGa                                                                                     >  2:610445/1‑101 (MQ=255)


                 tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAg                                                                                    >  1:7764089/1‑101 (MQ=255)


                  gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAgg                                                                                   >  2:2091460/1‑101 (MQ=255)


                  gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAgg                                                                                   >  1:8635788/1‑101 (MQ=255)


                  gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAgg                                                                                   >  2:5608431/1‑101 (MQ=255)


                  gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAgg                                                                                   <  2:6729354/101‑1 (MQ=255)


                   tgtccccgccgccgcGGCGGCGCCCCTTTTTCCGGGGGGAAA.CGCGCGGGGGGTTGG.CGCGGCGGCG.AAGGGGGGGGGCGCCCCCCCCCGAAGCGCCTGAg                                                                                    <  2:4397578/92‑1 (MQ=255)


                   tgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGgc                                                                                  >  2:3338567/1‑101 (MQ=255)


                       cTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcgg                                                                              >  2:3766523/1‑101 (MQ=255)


                       cTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcgg                                                                              >  2:1769306/1‑101 (MQ=255)


                        ttCGCGCCGGGGCAGGCCGGCTTTTCCTGGGGCGAAA.CGC.GGGGGGGGG.G.CGCGTCGCCGAAGGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcggc                                                                             <  1:4166901/101‑1 (MQ=255)


                          cgcgCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGcggcgg                                                                           >  2:7272983/1‑101 (MQ=255)


                              ccgcGGCAGCCCCGTTTTTCCTTGGGCGAAA.CGC.GGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCtt                                                                       <  1:8309615/101‑1 (MQ=255)


                              ccgcAGCGGGCCCGCTTTTCCTTGGGCGAAC.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCtt                                                                       <  1:2715848/101‑1 (MQ=255)


                               cgcAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCGTg                                                                      >  2:911621/1‑101 (MQ=255)


                                gcagcaGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgc                                                                     >  1:1297022/1‑101 (MQ=255)


                                gcagcaGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgc                                                                     >  1:6061576/1‑101 (MQ=255)


                                 cggcgggCCCGTTTTCCCTGGGGCGAAC.CGC.GGGGGGGTG.G.CGCGCCGGCGCAGGGGGGGGGCGGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                                    <  1:703489/96‑1 (MQ=255)


                                 cagcagGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                                    >  1:7937093/1‑101 (MQ=255)


                                 cagcagGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                                    >  1:3827127/1‑101 (MQ=255)


                                  cgcagGGCCGCCTCTGCATGGGCCAAACCGC.GGGGGGGTC.C.CGGGT.GGCGCAAAGGGGGGGCAAGCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                                    <  1:6659043/100‑1 (MQ=12)


                                   gcagGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcccg                                                                  <  2:8088470/101‑1 (MQ=255)


                                   gcagGCCCGCTTTTCCTTGGGCGAAA.CG..CGGGGGGTT.GCCGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcccg                                                                  <  1:5283461/101‑1 (MQ=37)


                                    cagGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                                 >  2:7562987/1‑101 (MQ=255)


                                     agGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGGT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCGTgcccgcc                                                                >  2:6964642/1‑101 (MQ=255)


                                             ttttCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGGTTGCCCGCCCCACGAAg                                                        >  1:3512125/1‑101 (MQ=38)


                                              tttCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGCAgg                                                       >  1:5093434/1‑101 (MQ=255)


                                                tCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGTAGCGCCTGGGGCGCGGCGGGCTGCGCCGCCCAACGAAGgcg                                                     >  2:2930881/1‑101 (MQ=37)


                                                  cTTGGGCGAAC.CGC.CGGGGGGTG.G.CGCGTCAGCGCAAGCGGGGGCCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCa                                                   <  2:2447945/101‑1 (MQ=25)


                                                  cTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCa                                                   <  2:7126872/101‑1 (MQ=255)


                                                  cTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCACGCCCAACGCAGGCGCa                                                   >  2:8243253/1‑101 (MQ=255)


                                                        cGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAaccacc                                             >  1:8850441/1‑101 (MQ=255)


                                                         gAAC.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                                            <  2:6778750/101‑1 (MQ=255)


                                                         gAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCt                                            >  1:74411/1‑101 (MQ=255)


                                                          aaa.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCtt                                           >  2:2897084/1‑101 (MQ=255)


                                                           aa.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTc                                          >  1:2385132/1‑101 (MQ=255)


                                                            acccgggggggggTG.G.CGCGCCCGCGCGGGGGGGGGCCCGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCtt                                           <  2:3015662/94‑1 (MQ=18)


                                                            accGC.GGGGGGGTT.G.CGCGTCGGCGCAAGCGGGGGGCAGCG.CCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTc                                          <  2:1449592/99‑1 (MQ=21)


                                                            accGC.GGGGGGGTG.G.CGGGCCGGCG.AAGGGGGGGGGCGCCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTc                                          <  1:6329805/99‑1 (MQ=18)


                                                             ccGC.GGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                                         <  1:5429299/100‑1 (MQ=255)


                                                              cGC.GGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                                        <  1:8175647/101‑1 (MQ=255)


                                                              cGC.GGGGGGGTT.G.CGCGGCGGGGCAGGGGGGGGGCAGCG.CCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                                        <  2:7873198/101‑1 (MQ=21)


                                                              cGC.CGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                                        >  1:4285807/1‑101 (MQ=255)


                                                                  gggggggTT.G.CGGGCCAGCGAAGGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                                     <  1:2665866/100‑1 (MQ=21)


                                                                  cGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                                     >  2:3883364/1‑101 (MQ=255)


                                                                   ggggggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                                    >  1:3998550/1‑101 (MQ=255)


                                                                      gggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCtt                                 >  2:8778412/1‑101 (MQ=255)


                                                                       ggTT.G.CGCGTCGGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTc                                <  2:1296990/101‑1 (MQ=255)


                                                                        gTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                               >  2:6022883/1‑101 (MQ=255)


                                                                        gTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCCGCTTCg                               >  2:169079/1‑101 (MQ=255)


                                                                          t.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGAt                             >  2:1525049/1‑101 (MQ=255)


                                                                          gtggtgcgCCAGCGCAGGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                               <  2:5823658/96‑1 (MQ=21)


                                                                              cgggTCAGCGCAAGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcc                           <  1:2249060/98‑1 (MQ=255)


                                                                              cgcgTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcc                           >  2:3548441/1‑101 (MQ=255)


                                                                               gctccggcgcAAGCGGGGGCCA.CCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATccc                          <  1:7333324/95‑1 (MQ=25)


                                                                               gcgTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGCTccc                          >  2:2292304/1‑101 (MQ=25)


                                                                               gcgTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATccc                          >  1:4617013/1‑101 (MQ=255)


                                                                               gcgTCAGCGCAAGCGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATccc                          <  2:1206348/101‑1 (MQ=255)


                                                                                  gcAGCGCAAGCGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac                       <  2:3392635/100‑1 (MQ=255)


                                                                                   cggcccagggggggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacc                      <  1:7563311/93‑1 (MQ=25)


                                                                                      cgcAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgc                   >  2:7488944/1‑101 (MQ=255)


                                                                                       gcAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcg                  >  2:4782634/1‑101 (MQ=255)


                                                                                         caGGGGGCGGCCCCCCCCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgc                 <  2:6240650/100‑1 (MQ=37)


                                                                                         aaGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGcgcg                >  1:4109741/1‑101 (MQ=255)


                                                                                          agcggggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgccg               >  1:168304/4‑99 (MQ=255)


                                                                                             ggggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcgg            >  1:3306557/1‑101 (MQ=255)


                                                                                             ggggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcgg            >  1:2874509/1‑101 (MQ=255)


                                                                                             ggggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcgg            >  1:5899327/1‑101 (MQ=255)


                                                                                              gggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcggc           >  2:5154600/1‑101 (MQ=255)


                                                                                              gggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcggc           >  2:4483196/1‑101 (MQ=255)


                                                                                               ggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcggcg          >  2:844910/1‑101 (MQ=255)


                                                                                                gggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcgacac         <  2:3983700/101‑5 (MQ=255)


                                                                                                gggCAGCG.CCCCACGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACGTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGa         >  1:6505332/1‑101 (MQ=255)


                                                                                                  gCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAAt       >  2:8933954/1‑101 (MQ=255)


                                                                                                     gcccccccccGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTc     <  2:4098152/98‑1 (MQ=255)


                                                                                                       gcCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGACGGCGAATTCCt   <  1:8070965/101‑1 (MQ=255)


                                                                                                       ccccccccGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTCCt   <  1:3389390/100‑1 (MQ=255)


CGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGC.CGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGCGCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTCCTC  >  NC_014034/2559835‑2560035

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG