Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,617,778 Δ1 bp 100% intergenic (‑152/‑462) RCAP_rcc02439 ← / → RCAP_rcc02440 hypothetical protein/AsnC/Lrp family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,617,7780C→.100.0% 25.6 342intergenic (‑152/‑462)RCAP_rcc02439/RCAP_rcc02440hypothetical protein/AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (192/137):  ref base (2/11):  total (194/148)
Fisher's exact test strand distribution p-value = 2.94e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.70e-10

Only 100 of 409 total aligned reads displayed.

GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAG  >  NC_014034/2617678‑2617877
                                                                                                    |                                                                                                   
gcggggATTAAGGCCGGAATTCGGGCAGCAGACACAGCGCAGCTGACAGCTGCCTACGATGTCGAAAAGCAGCGTGGACCCAGACAGCGGCCTCCCGGCTc                                                                                                     >  2:1645369/1‑101 (MQ=255)
 cggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcc                                                                                                    <  2:6291953/101‑1 (MQ=255)
  ggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGGCAGCGGCCTCCCGGCTccc                                                                                                   >  1:4174780/1‑101 (MQ=255)
  ggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccc                                                                                                   <  1:6973398/101‑1 (MQ=255)
  ggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccc                                                                                                   <  2:657177/101‑1 (MQ=255)
  ggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccc                                                                                                   >  1:7630794/1‑101 (MQ=255)
   gggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccc                                                                                                  <  2:5714916/101‑1 (MQ=255)
   gggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACACCGACAGCGGCCTCCCGGCTcccc                                                                                                  >  2:4032543/1‑101 (MQ=255)
     gATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccc                                                                                                <  1:3627395/101‑1 (MQ=255)
      aTCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccc                                                                                               >  1:1001406/1‑101 (MQ=255)
       tCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccccc                                                                                              <  1:7846471/101‑1 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                             >  1:2457338/1‑100 (MQ=255)
         aaGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCTACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgg                                                                                            >  1:897254/1‑99 (MQ=255)
         aaGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgg                                                                                            >  1:3828689/1‑99 (MQ=255)
         aaGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgc                                                                                            >  2:2197853/1‑99 (MQ=255)
           ggCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggcc                                                                                          >  1:6723115/1‑97 (MQ=255)
           ggCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggcc                                                                                          >  1:1505778/1‑97 (MQ=255)
              cGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaaa                                                                                      >  1:8532332/1‑101 (MQ=255)
              cGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaaa                                                                                      >  1:3017910/1‑101 (MQ=255)
              cGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaaa                                                                                      <  2:4948338/101‑1 (MQ=255)
               ggAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT..CCCCCCCGGCCAAAGc                                                                                    >  1:1426363/1‑101 (MQ=255)
                 aaTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcc                                                                                   >  2:4624155/1‑101 (MQ=255)
                 aaTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcc                                                                                   >  1:4031809/1‑101 (MQ=255)
                  aTTCGGGCAGCCGACACAGCGCAGCTGACAGCCACCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGccc                                                                                  >  1:6439872/1‑101 (MQ=255)
                   ttCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcccc                                                                                 <  1:8426192/101‑1 (MQ=255)
                    tCGGGCAGCGGGCACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCg                                                                                <  1:5391533/101‑1 (MQ=255)
                    tCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCg                                                                                <  2:7966830/101‑1 (MQ=255)
                     cGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCgg                                                                               >  2:6944263/1‑101 (MQ=255)
                      gggCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGc                                                                              >  2:1823178/1‑101 (MQ=255)
                       ggCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGcc                                                                             <  2:1950657/101‑1 (MQ=255)
                       ggCAGCCGACACAGCGCAGCTGACAGCCCCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGcc                                                                             <  1:7957400/101‑1 (MQ=255)
                         cAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCg                                                                           <  1:7759706/101‑1 (MQ=255)
                             cGACACAGCGCAGCTGGCAGCCGCGGACGAGGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTc                                                                       <  1:1741850/101‑1 (MQ=255)
                             cGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTc                                                                       <  2:8034618/101‑1 (MQ=255)
                               acacAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGt                                                                     >  1:1611548/1‑101 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGCCGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                    <  2:1475887/101‑1 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                    >  2:8151365/1‑101 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                    <  1:4699567/101‑1 (MQ=255)
                                         gCTGACAGCCGCAGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCACCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcggc                                                           >  1:8191213/1‑101 (MQ=255)
                                          cTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcggcg                                                          <  1:2002832/101‑1 (MQ=255)
                                          cTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcggcg                                                          <  2:4342470/101‑1 (MQ=255)
                                           tGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcggcgc                                                         >  2:8769384/1‑100 (MQ=255)
                                           tGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcggcgc                                                         >  1:1806086/1‑100 (MQ=255)
                                           tGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGa                                                         >  2:51207/1‑101 (MQ=255)
                                            gACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGAc                                                        >  2:6214008/1‑101 (MQ=255)
                                            gACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGAc                                                        <  1:2327285/101‑1 (MQ=255)
                                              cAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACgg                                                      <  1:5644799/101‑1 (MQ=255)
                                               aGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGa                                                     >  1:7296678/1‑101 (MQ=255)
                                               aGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGa                                                     >  2:1045/1‑101 (MQ=255)
                                                gccgccGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAt                                                    >  1:7507167/1‑101 (MQ=255)
                                                gccgccGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAt                                                    <  1:1280735/101‑1 (MQ=255)
                                                   gccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtgtg                                                 >  2:5066504/1‑101 (MQ=255)
                                                   gccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtgtg                                                 <  1:9090288/101‑1 (MQ=255)
                                                   gccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtgtg                                                 <  2:1197674/101‑1 (MQ=255)
                                                    ccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATgtgt                                                <  2:3242149/101‑1 (MQ=255)
                                                     cgacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTc                                               <  1:5134982/101‑1 (MQ=255)
                                                     cgacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTc                                               >  2:3977757/1‑101 (MQ=255)
                                                      gacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCa                                              >  1:6603942/1‑101 (MQ=255)
                                                      gacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCa                                              >  2:774182/1‑101 (MQ=255)
                                                       acgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCAt                                             <  1:4219469/101‑1 (MQ=255)
                                                        cgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATg                                            >  1:8084135/1‑101 (MQ=255)
                                                          aTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGTGCCGGACGGGTCATggg                                          >  2:7304977/1‑101 (MQ=255)
                                                          aTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATggg                                          >  2:5535043/1‑101 (MQ=255)
                                                            gTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCt                                        >  1:3229503/1‑101 (MQ=255)
                                                               gAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTc                                     <  2:1705354/101‑1 (MQ=255)
                                                                aaaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCg                                    <  1:6253718/101‑1 (MQ=255)
                                                                  aTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCa                                  <  1:5883305/101‑1 (MQ=255)
                                                                   tGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAg                                 <  2:720318/101‑1 (MQ=255)
                                                                       gggTGGACAGCGACCGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTc                             <  1:2197915/99‑1 (MQ=255)
                                                                        cGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTcc                            <  1:8708625/101‑1 (MQ=255)
                                                                         ggggACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTccc                           <  2:9085301/99‑1 (MQ=255)
                                                                         gTGGACAGCGTCAGCGGCCTCCCGGCTCCCCCCCCC.GCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGTTGGGCCATGGGCGTTCGCAGCCTccc                           >  2:5942331/1‑101 (MQ=255)
                                                                         gTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTccc                           <  2:7857240/101‑1 (MQ=255)
                                                                          tGGACAGCGACAGCGGCCCCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCCGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCg                          <  2:2559285/101‑1 (MQ=255)
                                                                           ggtcagcctgagcgGCCTCCCGG.TCCCCCCCCCGGCCAAAGCCGCGGCCCGCCTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGa                         <  2:5443027/91‑1 (MQ=255)
                                                                              cagcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGt                      >  1:392373/1‑101 (MQ=255)
                                                                                  gacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTTATGGGCTTTCGCAGCCTCCCGAAGTTgcg                  >  1:4556442/1‑101 (MQ=255)
                                                                                  gacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcg                  >  2:8280302/1‑101 (MQ=255)
                                                                                  gacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcg                  >  2:2424515/1‑101 (MQ=255)
                                                                                    cagggGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCa                >  1:8823512/1‑101 (MQ=255)
                                                                                    cagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCa                >  2:301509/1‑101 (MQ=255)
                                                                                    cagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCa                <  2:1281424/101‑1 (MQ=255)
                                                                                    cagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCa                >  1:6855331/1‑101 (MQ=255)
                                                                                      gcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAc              >  2:7887339/1‑101 (MQ=255)
                                                                                      gcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAc              <  2:6861084/101‑1 (MQ=255)
                                                                                       cgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTTGCAGCCTCCCGAAGTTGCGCAAcc             >  1:2614695/1‑101 (MQ=255)
                                                                                       cgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAcc             >  1:934809/1‑101 (MQ=255)
                                                                                       cgGACTCGCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGGTGCGCAAcc             >  2:3331164/1‑101 (MQ=255)
                                                                                        ggCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc            <  1:690114/101‑1 (MQ=255)
                                                                                        ggCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc            >  1:6357353/1‑101 (MQ=255)
                                                                                        ggCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAcac            >  2:8585582/1‑99 (MQ=255)
                                                                                         gCCTCCCGGC.CCCCCCCCCGCCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcg           <  1:7202164/101‑1 (MQ=255)
                                                                                          ccTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcgc          <  2:7624799/101‑1 (MQ=255)
                                                                                          ccTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcgc          >  2:5289925/1‑101 (MQ=255)
                                                                                             cccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGc       >  1:607770/1‑101 (MQ=255)
                                                                                             cccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGc       >  1:836206/1‑101 (MQ=255)
                                                                                             cccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGc       >  1:54904/1‑101 (MQ=255)
                                                                                               cGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTg     >  2:3527749/1‑101 (MQ=255)
                                                                                                  gccccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCa   <  2:2787667/99‑1 (MQ=255)
                                                                                                  gccccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCa   <  1:2791922/99‑1 (MQ=255)
                                                                                                    |                                                                                                   
GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAG  >  NC_014034/2617678‑2617877

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG