Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,205 Δ1 bp 0.9% intergenic (‑181/+95) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2050G→. 0.9% 2.7 ‑2.1 ‑1.2455intergenic (‑181/+95)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (221/230):  total (221/234)
Fisher's exact test strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.34e-02

Only 100 of 481 total aligned reads displayed.

TCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAA  >  NC_014034/2640109‑2640305
                                                                                                   |                                                                                                      
 cAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGGCCCCCTCTTT.GC.CTTCGGCCAAGTCACCCCCCCAGGGATTTTGAGGCAGATTAAAA.ACCAt                                                                                                   >  1:6555930/1‑101 (MQ=255)
  aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCACGATG.AAAGACCATg                                                                                                  >  2:346621/1‑101 (MQ=21)
   aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..g                                                                                               <  2:8519836/101‑1 (MQ=25)
   aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..g                                                                                               <  2:8268564/101‑1 (MQ=25)
       tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGG.CTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCCTG..GGGTc                                                                                           >  1:2771956/1‑101 (MQ=21)
           gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCt                                                                                       <  2:7145618/101‑1 (MQ=21)
            cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCtt                                                                                      <  1:8549792/101‑1 (MQ=21)
             ttttGCGCAGGCAAACGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCACG..GGTTCATCTTg                                                                                     <  2:6693678/101‑1 (MQ=21)
              tttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGc                                                                                    <  2:7390367/101‑1 (MQ=25)
               ttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGcc                                                                                   >  2:2694469/1‑101 (MQ=21)
               ttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGcc                                                                                   <  2:4710028/101‑1 (MQ=25)
                tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGCGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCt                                                                                  <  2:4441134/101‑1 (MQ=21)
                 gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                                 <  1:4900924/101‑1 (MQ=25)
                 gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                                 <  1:5205488/101‑1 (MQ=25)
                  cgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCa                                                                                <  1:911566/101‑1 (MQ=25)
                     aGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAat                                                                             <  2:2580534/101‑1 (MQ=21)
                       ccgaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATc                                                                          >  1:3090450/4‑101 (MQ=17)
                        cgaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                                                         >  2:3793075/3‑101 (MQ=17)
                         gaaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGAC..GG..GGTTTATCTTGCCCCAAATATccc                                                                        >  1:3313207/2‑101 (MQ=17)
                          aaaGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                                        <  1:6121047/101‑1 (MQ=21)
                          aaaGGGGGGCGCTGCCCCCCCC.TT.GGCCTTCGGCCAATTTACCCCCCGAGGCATTTGGAGCAAGATGAAAA.ACCATG..GGTTCATCTTTCCTCAAATATccc                                                                        >  1:5773598/1‑101 (MQ=21)
                          aaaAGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                                       >  1:3685126/1‑101 (MQ=17)
                           aaGGGGGGCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCg                                                                       <  2:7566000/101‑1 (MQ=21)
                             agggggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                                    >  1:99828/2‑101 (MQ=18)
                             agggggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                                    >  2:1322702/2‑101 (MQ=18)
                              gggggCGCTG.CCCCCTC.TT.GGCCTTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggg                                                                  <  1:7814630/101‑1 (MQ=2)
                               ggggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCggggg                                                                   <  1:4324482/101‑1 (MQ=25)
                               ggggCGCTG.CCCCCTC.TT.GGCCTTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGa                                                                 <  2:3115691/101‑1 (MQ=2)
                                gggCGCTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                                 <  2:8851178/101‑3 (MQ=18)
                                  gcgcTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                               <  1:3905923/101‑5 (MQ=21)
                                  gcgcTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                               <  1:4542479/101‑5 (MQ=21)
                                   cgcTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                               >  2:3912708/1‑101 (MQ=25)
                                    gcTGCCCCCCTC.TT.GGCCTTCGGGCCAATCAACCCCCCCGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTTCTCTTCGCTCAAACATTCCGGGGGGAggc                                                              >  2:3021254/1‑99 (MQ=9)
                                    gcTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGGGCAAGATG.AAAGACCATG..GGCTCATCTGGCGGCACATATCCCaagggggctc                                                              >  2:4261927/1‑97 (MQ=12)
                                    gcTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                              >  1:7960366/1‑101 (MQ=25)
                                     cTGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                             <  2:1909774/101‑1 (MQ=25)
                                      tGCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                            <  2:7912331/101‑1 (MQ=25)
                                       gCCCCCCTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                           <  2:550863/101‑1 (MQ=37)
                                         cccccTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                         >  2:3224775/1‑101 (MQ=25)
                                         cccccTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                         >  2:4132451/1‑101 (MQ=37)
                                         atctctc.tT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                         <  2:5119532/97‑1 (MQ=25)
                                          ccccTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                        >  1:1422510/1‑101 (MQ=37)
                                           cccTC.TT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                       <  2:7960026/101‑1 (MQ=37)
                                              tc.tT.GGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                    <  1:4157754/101‑1 (MQ=37)
                                                  ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                  >  1:762615/6‑101 (MQ=37)
                                                   tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGNACggg                                                 >  1:2043381/5‑101 (MQ=37)
                                                   tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                 >  1:8167670/5‑101 (MQ=37)
                                                     gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                               >  1:4711634/3‑101 (MQ=37)
                                                     gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                               <  2:528230/99‑1 (MQ=37)
                                                       cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                             >  2:1159893/1‑101 (MQ=37)
                                                       cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                             <  2:5663938/101‑1 (MQ=255)
                                                       cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                             >  2:4542654/1‑101 (MQ=37)
                                                       cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                             >  1:644422/1‑101 (MQ=37)
                                                       cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTAATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                             >  1:544283/1‑101 (MQ=37)
                                                         tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                           <  2:151925/101‑1 (MQ=255)
                                                         tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                           <  2:2350588/101‑1 (MQ=255)
                                                         tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                           <  1:1764971/101‑1 (MQ=255)
                                                         tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                           >  2:2820391/1‑101 (MQ=37)
                                                         tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                           >  1:5089636/1‑101 (MQ=37)
                                                           ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                         <  1:346629/101‑1 (MQ=255)
                                                              cAATTCACCCCCCGAGGTATTTGGGGAAAGATG.AAAGACCATG..GGCTCATCTTGCCTCAAATATCCCCGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcca                                      >  1:62559/1‑100 (MQ=25)
                                                              cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                      >  2:1882129/1‑101 (MQ=37)
                                                                aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                    >  1:1093358/1‑101 (MQ=37)
                                                                 ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                   >  2:9118210/1‑101 (MQ=25)
                                                                  tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                  >  1:1196910/4‑101 (MQ=25)
                                                                   caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                 >  1:2242472/3‑101 (MQ=255)
                                                                   caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                 >  1:5593669/3‑101 (MQ=255)
                                                                    cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                <  1:2820490/100‑1 (MQ=255)
                                                                     ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                               <  1:3037537/101‑1 (MQ=255)
                                                                       ccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                             >  2:7793099/1‑101 (MQ=255)
                                                                         ccGACGTAGTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTTCCTCAAATACCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                           <  1:2929819/101‑1 (MQ=37)
                                                                             ggTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                       >  2:8158060/1‑101 (MQ=255)
                                                                               tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCGTtcg                     >  1:7341864/1‑100 (MQ=25)
                                                                                aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                    >  1:5318047/1‑101 (MQ=255)
                                                                                aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                    <  2:8754053/101‑1 (MQ=255)
                                                                                aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                    >  2:4996385/1‑101 (MQ=255)
                                                                                aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                    >  1:866456/1‑101 (MQ=255)
                                                                                  ttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                  >  2:7012064/1‑101 (MQ=255)
                                                                                    ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                >  1:4642810/1‑101 (MQ=255)
                                                                                     gagcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg               >  1:3727543/4‑101 (MQ=255)
                                                                                       gcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAGATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt             <  2:5899131/100‑1 (MQ=255)
                                                                                       gcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt             <  1:8291119/100‑1 (MQ=255)
                                                                                        cAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc            >  2:2080902/1‑101 (MQ=255)
                                                                                        cAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc            >  2:6815769/1‑101 (MQ=255)
                                                                                         aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc           >  2:5403565/1‑101 (MQ=255)
                                                                                         aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc           >  1:2570351/1‑101 (MQ=255)
                                                                                         aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc           >  1:1341909/1‑101 (MQ=255)
                                                                                         aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc           >  1:8865344/1‑101 (MQ=255)
                                                                                         aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCCCCCGGGCCTTTCTCAAGCGtccg           >  1:6655238/1‑98 (MQ=25)
                                                                                          aGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg          >  1:6882222/1‑101 (MQ=255)
                                                                                             atgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc        >  2:6156111/4‑101 (MQ=255)
                                                                                             atgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc        >  2:4802669/4‑101 (MQ=255)
                                                                                             atgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc        >  2:2485886/4‑101 (MQ=255)
                                                                                              tgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt       >  2:3385616/3‑101 (MQ=255)
                                                                                                 aaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg    <  2:644412/101‑1 (MQ=255)
                                                                                                 aaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg    >  1:7061158/1‑101 (MQ=255)
                                                                                                 aaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCACGGGTCTTTCTCAAGCGTTCCGGCTCAg    >  2:6269522/1‑101 (MQ=255)
                                                                                                   gACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa  <  1:7141329/101‑1 (MQ=255)
                                                                                                   gACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa  <  2:3557848/101‑1 (MQ=255)
                                                                                                   gACCATG..GGTTCATCTTGACTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa  <  2:6555670/101‑1 (MQ=255)
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TCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAA  >  NC_014034/2640109‑2640305

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG