Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,207 Δ1 bp 11.0% intergenic (‑183/+93) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2070C→.11.0% 156.1 ‑2.9 ‑0.0465intergenic (‑183/+93)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (32/19):  ref base (192/214):  total (225/240)
Fisher's exact test strand distribution p-value = 5.25e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.56e-01

Only 100 of 494 total aligned reads displayed.

TCAACCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GG.CTT.CGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACC  >  NC_014034/2640109‑2640307
                                                                                                      |                                                                                                      
   aCCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCCCCCCCCGAGGTATTTGGAGCAATAT.TAAAGACCAAgg                                                                                                   >  2:4157635/1‑101 (MQ=21)
   aCCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                   <  2:6289534/101‑1 (MQ=21)
   aCCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                   <  2:8519836/101‑1 (MQ=25)
   aCCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                   <  2:8268564/101‑1 (MQ=25)
      gTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGGCGCCCCCCCCCTT.GC.CTTCCGGCCAA.TCACCCCCCCCGGCAGTTTGAGCGAGATCAAAA.ACCATGGG..tt                                                                                              >  1:7501151/1‑101 (MQ=255)
      gTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..tt                                                                                              <  2:6832851/101‑1 (MQ=25)
       tGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGG.CTT.CGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCCTGGG..GTc                                                                                             >  1:2771956/1‑101 (MQ=21)
         gcgcTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCAt                                                                                           <  1:6736064/101‑1 (MQ=25)
         gcgcTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCAt                                                                                           <  2:789557/101‑1 (MQ=25)
           gcTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCt                                                                                         <  2:7145618/101‑1 (MQ=21)
              tttGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGc                                                                                      <  2:7390367/101‑1 (MQ=25)
               ttGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGcc                                                                                     <  2:6732890/101‑1 (MQ=25)
                tGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCt                                                                                    <  1:6159898/101‑1 (MQ=25)
                ggcgaAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCC.ATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTc                                                                                   <  2:6172181/100‑1 (MQ=21)
                    cAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCaaa                                                                                <  2:3178298/101‑1 (MQ=25)
                     cccctaaa.AGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAata                                                                              <  2:2124653/96‑1 (MQ=17)
                       gCAAAA.GGGGGGCCCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAatat                                                                             <  1:3327943/101‑1 (MQ=21)
                        cAAAA.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATc                                                                            <  2:8102212/101‑1 (MQ=21)
                         gaaa.AGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATccc                                                                          <  2:4617937/100‑1 (MQ=17)
                         aaaa.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATcc                                                                           >  1:4907522/1‑101 (MQ=21)
                          aaa.AGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATCCCg                                                                         >  1:7898947/1‑101 (MQ=17)
                          aaa.AGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATCCCg                                                                         <  2:5918091/101‑1 (MQ=17)
                           aa.GGGGGGCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCg                                                                         <  2:7566000/101‑1 (MQ=21)
                            aaagggggCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCCAATATTCCgg                                                                        >  2:4485477/4‑101 (MQ=17)
                                ggggCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCggggg                                                                     >  2:4307349/1‑101 (MQ=25)
                                ggggCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCggggg                                                                     <  1:4324482/101‑1 (MQ=25)
                                ggggCGCTG.CCCCCTCTT.GGCCTT.TGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGa                                                                   <  2:343243/101‑1 (MQ=2)
                                 gggCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATCCCgggggtg                                                                   <  2:1067756/101‑3 (MQ=18)
                                 gggCGCTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATCCCgggggtg                                                                   <  2:8851178/101‑3 (MQ=18)
                                   gcgcTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATCCCgggggtgag                                                                 <  1:3905923/101‑5 (MQ=21)
                                   gcgcTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATCCCgggggtgag                                                                 <  1:4542479/101‑5 (MQ=21)
                                   gcgcTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAg                                                                  >  1:3700564/1‑101 (MQ=25)
                                   gcgcTGCCCCCCTCGT.GGCCTT.CGGCCAATTCGCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATCCCgggggtgag                                                                 <  1:6932700/101‑5 (MQ=18)
                                     gcTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGG..TTTATCTTGCCCCAAATATCCCgggggtgaggc                                                               >  1:359358/1‑95 (MQ=21)
                                      cTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                               <  1:6588502/101‑1 (MQ=25)
                                      cTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                               >  2:647789/1‑101 (MQ=25)
                                      cTGCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                               <  1:8871747/101‑1 (MQ=25)
                                        gCCCCCCTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                             <  2:550863/101‑1 (MQ=37)
                                           ccccTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                          >  1:1422510/1‑101 (MQ=37)
                                            cccTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                         <  1:5114776/101‑1 (MQ=37)
                                             ccTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                        <  2:3066294/101‑1 (MQ=37)
                                             ccTCTT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                        <  2:3988275/101‑1 (MQ=37)
                                              ctctT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                       <  1:8371097/101‑1 (MQ=37)
                                              ctctT.GGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCATCAAATATCCCGGGGGGAGTCCTGAAAgat                                                       >  1:651777/1‑99 (MQ=25)
                                               tctT.GGCCTT.CGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                      <  1:4157754/101‑1 (MQ=37)
                                                   tggccTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGNACggg                                                   >  1:2043381/5‑101 (MQ=37)
                                                     gccTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                 <  2:528230/99‑1 (MQ=37)
                                                       cTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                               <  1:4739177/101‑1 (MQ=255)
                                                         t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                             >  1:941515/1‑101 (MQ=37)
                                                         t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                             <  1:5227328/101‑1 (MQ=255)
                                                         t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                             <  2:151925/101‑1 (MQ=255)
                                                         t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                             >  1:7725284/1‑101 (MQ=37)
                                                         t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                             <  1:1764971/101‑1 (MQ=255)
                                                         t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                             <  1:5809196/101‑1 (MQ=255)
                                                           cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                            >  2:5140365/1‑101 (MQ=255)
                                                               cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                        >  2:1882129/1‑101 (MQ=37)
                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                      >  1:1093358/1‑101 (MQ=37)
                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                      >  2:3266863/1‑101 (MQ=37)
                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                      >  1:5471368/1‑101 (MQ=37)
                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                      >  1:873633/1‑101 (MQ=37)
                                                                  ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                     >  2:9118210/1‑101 (MQ=25)
                                                                  ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                     >  2:5052234/1‑101 (MQ=255)
                                                                   tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                    >  1:1196910/4‑101 (MQ=25)
                                                                   tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                    <  2:5697428/98‑1 (MQ=255)
                                                                   tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                    <  1:5725110/98‑1 (MQ=255)
                                                                   tcaccccacGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                    >  2:3503572/4‑101 (MQ=25)
                                                                    caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                   >  1:7289234/3‑101 (MQ=255)
                                                                    caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                   >  1:3129534/3‑101 (MQ=255)
                                                                     cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                  <  1:2820490/100‑1 (MQ=255)
                                                                     accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                  <  2:2782350/100‑1 (MQ=255)
                                                                     accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                  <  1:2577230/100‑1 (MQ=255)
                                                                     accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                  >  1:3041456/2‑101 (MQ=255)
                                                                        ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                               <  2:2601930/101‑1 (MQ=255)
                                                                          ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                             <  2:7947076/101‑1 (MQ=255)
                                                                          ccGACGTAGTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTTCCTCAAATACCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                             <  1:2929819/101‑1 (MQ=37)
                                                                           cGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                            >  2:4821013/1‑101 (MQ=255)
                                                                           cGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                            <  2:192220/101‑1 (MQ=255)
                                                                           cGAGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                            <  1:6341211/101‑1 (MQ=255)
                                                                             aGGTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                          >  1:8184897/1‑101 (MQ=255)
                                                                              ggTATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                         <  2:135509/101‑1 (MQ=255)
                                                                                tATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                       >  1:8591302/1‑101 (MQ=255)
                                                                                tATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                       >  2:3848884/1‑101 (MQ=255)
                                                                                tATTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTtct                       >  1:7112251/1‑101 (MQ=255)
                                                                                 aTTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                      <  2:8754053/101‑1 (MQ=255)
                                                                                 aTTTGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                      >  2:2564527/1‑101 (MQ=255)
                                                                                   ttGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                    >  1:8001882/1‑101 (MQ=255)
                                                                                   ttGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                    >  2:7012064/1‑101 (MQ=255)
                                                                                    tGGAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                   >  2:2906232/1‑101 (MQ=255)
                                                                                     ggAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                  >  1:3805831/1‑101 (MQ=255)
                                                                                     ggAGCAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                  >  1:4642810/1‑101 (MQ=255)
                                                                                         cAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc              >  2:237332/1‑101 (MQ=255)
                                                                                         cAAGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc              >  2:3568439/1‑101 (MQ=255)
                                                                                          aaGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc             >  2:5403565/1‑101 (MQ=255)
                                                                                          aaGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc             >  1:1341909/1‑101 (MQ=255)
                                                                                           aGATG.AAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg            <  1:2398946/101‑1 (MQ=255)
                                                                                                  aaGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg      <  2:4711485/101‑1 (MQ=255)
                                                                                                   aGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa     <  1:5331427/101‑1 (MQ=255)
                                                                                                    gACCATGGG..TTCATCTTGACTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa    <  2:6555670/101‑1 (MQ=255)
                                                                                                     aCCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc   >  2:1188982/1‑101 (MQ=255)
                                                                                                      ccATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAcc  <  2:7793053/101‑1 (MQ=255)
                                                                                                      |                                                                                                      
TCAACCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTT.GG.CTT.CGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGG..TTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACC  >  NC_014034/2640109‑2640307

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG