Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,208 Δ1 bp 11.0% intergenic (‑184/+92) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2080C→.11.0% 156.1 ‑3.3 ‑0.0463intergenic (‑184/+92)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (32/19):  ref base (190/219):  total (223/240)
Fisher's exact test strand distribution p-value = 3.68e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.81e-01

Only 100 of 499 total aligned reads displayed.

TCAACCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGG.CTT.CGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCG  >  NC_014034/2640109‑2640308
                                                                                                      |                                                                                                      
tCAACCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                                                                       <  1:712022/101‑1 (MQ=25)
 cAACCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGGCCCCCTCCTGGCCTT.CGGCCCATTCACCCCCCGAGGTATTTGGGGCAAAAAGAAAA.ACCAt                                                                                                      >  2:5733147/1‑101 (MQ=255)
 cAACCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAt                                                                                                      <  2:2383370/101‑1 (MQ=21)
   aCCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..g                                                                                                  <  2:6494830/101‑1 (MQ=25)
   aCCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..g                                                                                                  <  2:189009/101‑1 (MQ=25)
   aCCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..g                                                                                                  <  2:8519836/101‑1 (MQ=25)
    ccGTGGCGCTTTTGCGCAGGCAAAA.CGGGGGCGCTGCCCCCCTCTTGGCCCT.CCGCCAATTCACCCCCCCAGGCATTTGGAGCAAGATG.AAAGAGCATg..gg                                                                                                 >  2:3852988/1‑101 (MQ=21)
      gTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGGCGCCCCCCCCCTTGC.CTTCCGGCCAA.TCACCCCCCCCGGCAGTTTGAGCGAGATCAAAA.ACCATG..GGtt                                                                                               >  1:7501151/1‑101 (MQ=255)
        ggCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCa                                                                                             <  2:6308337/101‑1 (MQ=25)
        ggCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCa                                                                                             <  2:5540963/101‑1 (MQ=25)
                tGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCt                                                                                     <  1:6159898/101‑1 (MQ=25)
                    cAGGCAAAA.GGGGGGGGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCAAGGTATTTGGAGGAAGATGAAGA.ACCTTG..GGTTCATCTTGCCTCaaa                                                                                 >  2:5297274/1‑101 (MQ=21)
                      cccgaaa.AGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAatat                                                                              <  2:5972331/97‑1 (MQ=17)
                       ccgaaa.AGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATc                                                                             >  1:3090450/4‑101 (MQ=17)
                         gaaa.AGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGAC..GG..GGTTTATCTTGCCCCAAATATccc                                                                           >  1:3313207/2‑101 (MQ=17)
                         aaaa.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATcc                                                                            >  1:4907522/1‑101 (MQ=21)
                          aaa.GGGGGGCGCTGCCCCCCTCTTGGCCTT.CCGCCAATTCACCCCCCGAGGTATTTGGCGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                                           >  2:7403315/1‑101 (MQ=21)
                          aaa.GGGGGGCGCTGCCACCCTCTTGGCCTT.CGGACAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                                           <  2:6958684/101‑1 (MQ=25)
                          aaa.AGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                                          >  2:3996887/1‑101 (MQ=17)
                          aaa.AGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                                          >  1:7898947/1‑101 (MQ=17)
                          aaa.AGGGGGCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                                          >  1:3685126/1‑101 (MQ=17)
                            aaagggggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgg                                                                         >  1:1414557/4‑101 (MQ=17)
                            aaagggggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgg                                                                         >  2:2655349/4‑101 (MQ=17)
                            aaagggggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgg                                                                         >  2:6181212/4‑101 (MQ=17)
                             aagggggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCggg                                                                        >  1:133504/3‑101 (MQ=18)
                              agggggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                                       >  2:7574840/2‑101 (MQ=18)
                                ggggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCggggg                                                                      <  1:4324482/101‑1 (MQ=25)
                                ggggCGCTG.CCCCCTCTTGGCCTT.TGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGa                                                                    <  2:3525400/101‑1 (MQ=2)
                                 gggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                                    <  2:1067756/101‑3 (MQ=18)
                                 gggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                                    <  2:8851178/101‑3 (MQ=18)
                                  ggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                                                                   >  1:6834317/1‑98 (MQ=21)
                                  ggCGCTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                    <  2:4645515/101‑1 (MQ=25)
                                   gcgcTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                  <  1:3905923/101‑5 (MQ=21)
                                   gcgcTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                  <  1:4542479/101‑5 (MQ=21)
                                     gcTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGATGA....GGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                                <  2:3269523/101‑7 (MQ=21)
                                     gcTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                                 <  2:4758862/101‑1 (MQ=25)
                                     gcTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                                 <  2:5072898/101‑1 (MQ=25)
                                     gcTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                                 <  2:453995/101‑1 (MQ=25)
                                      cTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                <  1:8871747/101‑1 (MQ=25)
                                      cTGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                <  1:6588502/101‑1 (MQ=25)
                                       tGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                               <  2:7912331/101‑1 (MQ=25)
                                       tGCCCCCCTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                               >  2:4162317/1‑101 (MQ=25)
                                          cccccTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                            >  2:2574345/1‑101 (MQ=25)
                                          cccccACCTGGCCTT.CGGCCAATTCACCCCACGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                            <  1:2927739/101‑1 (MQ=25)
                                            cccTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                          >  1:281226/1‑101 (MQ=37)
                                            cccTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                          <  2:7960026/101‑1 (MQ=37)
                                            cccTCTTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                          <  1:5114776/101‑1 (MQ=37)
                                              ctctTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                        <  1:8371097/101‑1 (MQ=37)
                                              ctctTGGCCTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCATCAAATATCCCGGGGGGAGTCCTGAAAgat                                                        >  1:651777/1‑99 (MQ=25)
                                               tctTGGCCTT.CGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                       <  1:4157754/101‑1 (MQ=37)
                                                    gccTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                  >  1:2032158/3‑101 (MQ=37)
                                                      cTT.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                <  1:4739177/101‑1 (MQ=255)
                                                        t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                              >  1:941515/1‑101 (MQ=37)
                                                        t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                              <  1:5227328/101‑1 (MQ=255)
                                                        t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                              <  1:5809196/101‑1 (MQ=255)
                                                        t.CGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                              >  1:7725284/1‑101 (MQ=37)
                                                          cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                             <  1:3780685/101‑1 (MQ=255)
                                                          cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                             >  1:171767/1‑101 (MQ=255)
                                                           ggCCAATTCACCCCCCGNGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                            <  2:2783888/101‑1 (MQ=37)
                                                           ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                            <  1:346629/101‑1 (MQ=255)
                                                             ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                          <  2:1933213/101‑1 (MQ=255)
                                                              cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                         >  2:7067820/1‑101 (MQ=37)
                                                               aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGAACATG..GGTTTATCTTGCCTCAAATATCTCGGGGGGAAACAGGAAAGGAGGGGGGGCACGGccca                                        >  2:2931737/1‑100 (MQ=16)
                                                                aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                       >  1:5471368/1‑101 (MQ=37)
                                                                aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                       >  1:873633/1‑101 (MQ=37)
                                                                aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                       >  1:1093358/1‑101 (MQ=37)
                                                                 ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                      >  2:9118210/1‑101 (MQ=25)
                                                                 ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                      <  2:3188428/101‑1 (MQ=255)
                                                                  tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGCCGGGGGGCAGAGCCCCCCt                                     >  2:2299669/4‑101 (MQ=25)
                                                                  tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                     >  1:1196910/4‑101 (MQ=25)
                                                                  tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                     <  1:5725110/98‑1 (MQ=255)
                                                                   caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                    >  2:6797044/3‑101 (MQ=255)
                                                                   caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                    >  1:2242472/3‑101 (MQ=255)
                                                                    accccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                   >  1:4615711/2‑101 (MQ=255)
                                                                    accccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                   <  2:3433082/100‑1 (MQ=255)
                                                                     ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                  <  2:8331401/101‑1 (MQ=255)
                                                                     ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                  <  1:3037537/101‑1 (MQ=255)
                                                                       ccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                >  2:163233/1‑101 (MQ=255)
                                                                       ccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                >  2:7793099/1‑101 (MQ=255)
                                                                          cGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                             <  1:6341211/101‑1 (MQ=255)
                                                                            aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                           >  1:8184897/1‑101 (MQ=255)
                                                                             ggTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                          >  2:8158060/1‑101 (MQ=255)
                                                                             ggTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                          <  2:135509/101‑1 (MQ=255)
                                                                               tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                        >  1:8591302/1‑101 (MQ=255)
                                                                                aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCNCCTCCCGGGTCTTtctc                       >  2:3675953/1‑101 (MQ=255)
                                                                                aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                       <  2:8754053/101‑1 (MQ=255)
                                                                                aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                       >  2:4338923/1‑101 (MQ=255)
                                                                                  ttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                     >  1:8001882/1‑101 (MQ=255)
                                                                                  ttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                     <  2:5407743/101‑1 (MQ=255)
                                                                                   tGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                    <  2:7226558/101‑1 (MQ=255)
                                                                                         aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc              >  1:2570351/1‑101 (MQ=255)
                                                                                            gatgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg            <  2:3105355/97‑1 (MQ=255)
                                                                                             atgaaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc           >  2:516617/4‑101 (MQ=255)
                                                                                                aaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa        <  2:6620821/101‑1 (MQ=255)
                                                                                                aaaGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa        <  1:7039886/101‑1 (MQ=255)
                                                                                                  aGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa      <  1:5331427/101‑1 (MQ=255)
                                                                                                    aCCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc    >  2:1188982/1‑101 (MQ=255)
                                                                                                    aCCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc    <  1:2761264/101‑1 (MQ=255)
                                                                                                     ccATG..GGTTCATCTTGCCTCAAATATCGCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAcc   <  2:4903441/101‑1 (MQ=255)
                                                                                                      cATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg  >  1:7321501/1‑101 (MQ=255)
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TCAACCGTGGCGCTTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGG.CTT.CGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCG  >  NC_014034/2640109‑2640308

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG