Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,889,441 Δ1 bp 1.3% intergenic (+13/‑198) holC → / → thiB DNA polymerase III subunit chi/thiamine ABC transporter periplasmic thiamin‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,889,4410C→. 1.3% 6.0 0.0 ‑0.3392intergenic (+13/‑198)holC/thiBDNA polymerase III subunit chi/thiamine ABC transporter periplasmic thiamin‑binding protein
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (209/178):  total (212/180)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.31e-01

Only 100 of 464 total aligned reads displayed.

TCAGTGGAAGGCGCTGACCGGGGCGGGCACCGCGGCGCAATACTGGCGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGTTTTCG  >  NC_014034/2889341‑2889541
                                                                                                    |                                                                                                    
 cAGTGGAAGGCGCTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGcc                                                                                                     >  2:1796473/1‑101 (MQ=255)
  aGTGGAAGGCGCTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGccc                                                                                                    >  2:5254777/1‑101 (MQ=255)
     ggAAGGCGCTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGTAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGcccccc                                                                                                 >  1:2725451/1‑101 (MQ=255)
        aGGCGCTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCg                                                                                              <  1:8276794/101‑1 (MQ=255)
         ggCGCTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGc                                                                                             <  2:5706255/101‑1 (MQ=255)
          gcgcTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCa                                                                                            <  1:4448223/101‑1 (MQ=255)
          gcgcTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGAAGCGCCCCCTCGCGCCCCCCCCGCa                                                                                            >  2:3001110/1‑101 (MQ=255)
           cgcTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCaa                                                                                           <  2:6475964/101‑1 (MQ=255)
            gcTGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCaaa                                                                                          >  1:5781904/1‑101 (MQ=255)
             cTGCCCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCCCGCGCCCCCCCCGCaaaa                                                                                         <  2:3511080/101‑1 (MQ=255)
              tGACCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAac                                                                                        >  2:5363356/1‑101 (MQ=255)
                aCCGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAacac                                                                                      <  2:2235903/101‑1 (MQ=255)
                 ccGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCCCGCCCCCCCCGCAAAAcaca                                                                                     >  1:7245874/1‑101 (MQ=255)
                  cGGGGCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAg                                                                                    >  1:1904840/1‑101 (MQ=255)
                   ggggCGGGCACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGt                                                                                   <  2:1896836/101‑1 (MQ=255)
                     ggcgggcACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGttt                                                                                 <  1:5009077/101‑1 (MQ=255)
                      gcgggcACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGtttt                                                                                <  2:8706053/101‑1 (MQ=255)
                        gggcACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCg                                                                              <  2:8447736/101‑1 (MQ=255)
                           cACCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCtt                                                                           <  1:2327985/101‑1 (MQ=255)
                            aCCGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTg                                                                          >  1:5507734/1‑101 (MQ=255)
                             ccGCGGCGCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGc                                                                         <  2:5963004/101‑1 (MQ=255)
                               gcggcgCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAg                                                                       <  1:794976/101‑1 (MQ=255)
                               gcggcgCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAg                                                                       <  2:2098748/101‑1 (MQ=255)
                                cggcgCAAGACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGc                                                                      <  2:2446131/101‑1 (MQ=255)
                                 ggcgCAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCt                                                                     <  1:6634954/101‑1 (MQ=255)
                                  gcgcAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCtt                                                                    >  2:8850703/1‑101 (MQ=255)
                                    gcAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTg                                                                  <  1:4647646/101‑1 (MQ=255)
                                     cAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCACAACACAGTTTTCGCTTGCAGCTTTgg                                                                 >  2:3592140/1‑101 (MQ=255)
                                     cAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTgg                                                                 <  1:5254961/101‑1 (MQ=255)
                                     cAATACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTgg                                                                 <  2:3918421/101‑1 (MQ=255)
                                      aaTACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGgc                                                                >  2:2316715/1‑101 (MQ=255)
                                      aaTACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGgc                                                                <  1:6811079/101‑1 (MQ=255)
                                      aaTACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGgc                                                                >  1:386253/1‑101 (MQ=255)
                                       aTACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGgcg                                                               >  2:33028/1‑101 (MQ=255)
                                       aTACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGgcg                                                               >  2:7561193/1‑101 (MQ=255)
                                        tACTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGgcgc                                                              <  2:6086450/101‑1 (MQ=255)
                                          cTGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCgg                                                            >  2:6674658/1‑101 (MQ=255)
                                           tGGAGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCggg                                                           >  2:8355448/1‑101 (MQ=255)
                                            ggagcgAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCgggg                                                          >  2:5798208/4‑101 (MQ=255)
                                             gagcgAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGc                                                         <  1:4899161/99‑1 (MQ=255)
                                              agcgAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGcc                                                        <  1:9176532/100‑1 (MQ=255)
                                                ggAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCtt                                                      <  1:9016070/100‑1 (MQ=255)
                                                cgAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGTGGCCtt                                                      <  2:338349/101‑1 (MQ=255)
                                                 gAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCG.CCCCCCCGCCAAACACCGGTTTCGCTTGCAGCCTTGGCGCGGGGCCTTAt                                                    >  1:1735399/1‑101 (MQ=255)
                                                  aGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTAt                                                    <  2:6943877/101‑1 (MQ=255)
                                                  aGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGAAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTCTTCGCTTGCAGCTTTGGCGCGGGGCCTTAt                                                    >  1:3487781/1‑101 (MQ=255)
                                                   ggAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATg                                                   >  2:5502891/1‑101 (MQ=255)
                                                   ggAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATg                                                   >  1:1202151/1‑101 (MQ=255)
                                                   ggAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATg                                                   <  1:4121255/101‑1 (MQ=255)
                                                    gAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATgg                                                  >  1:4030913/1‑101 (MQ=255)
                                                     aaaGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCTGGGCCTTATGGt                                                 >  1:3176268/1‑101 (MQ=255)
                                                     aaaGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGt                                                 >  1:1459750/1‑101 (MQ=255)
                                                     aaaGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGt                                                 >  1:1605860/1‑101 (MQ=255)
                                                       aGCGGGCGCTGGCAGAAGAAGGCGGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTcc                                               >  2:7800168/1‑101 (MQ=255)
                                                       aGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTcc                                               <  1:4337120/101‑1 (MQ=255)
                                                        gCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCg                                              <  2:5096394/101‑1 (MQ=255)
                                                          gggCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGt                                            <  2:1115998/101‑1 (MQ=255)
                                                          gggCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGt                                            <  1:3049291/101‑1 (MQ=255)
                                                           ggCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGtc                                           >  2:3792667/1‑101 (MQ=255)
                                                            gcgcTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGtct                                          <  2:7033323/101‑1 (MQ=255)
                                                             cgcTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGtctc                                         >  1:1004149/1‑101 (MQ=255)
                                                              gcTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGtctcc                                        >  2:7315401/1‑100 (MQ=255)
                                                              gcTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCa                                        <  1:2147308/101‑1 (MQ=255)
                                                              gcTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCa                                        <  1:2477202/101‑1 (MQ=255)
                                                               cTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCAc                                       >  2:2571016/1‑101 (MQ=255)
                                                                tGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCAcc                                      >  2:8192867/1‑101 (MQ=255)
                                                                tGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCAcc                                      >  1:5895068/1‑101 (MQ=255)
                                                                tGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCAcc                                      >  1:3745935/1‑101 (MQ=255)
                                                                tGGCAGAAGAAGACCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCAcc                                      >  1:8075845/1‑101 (MQ=255)
                                                                 ggCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCt                                     <  2:4596359/101‑1 (MQ=255)
                                                                   cAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCg                                   >  1:8585581/1‑101 (MQ=255)
                                                                   cAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCCCACCCCg                                   >  2:2913981/1‑101 (MQ=255)
                                                                    agaagaAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCgg                                  >  2:265859/1‑101 (MQ=255)
                                                                    agaagaAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGGCCGGGCTCACCCCgg                                  >  2:4223565/1‑101 (MQ=255)
                                                                        gaagGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCCGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTg                              <  2:4364744/101‑1 (MQ=255)
                                                                        gaagGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTg                              >  1:6410739/1‑101 (MQ=255)
                                                                         aagGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGc                             >  2:8047633/1‑101 (MQ=255)
                                                                            gCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGctct                          <  2:8567324/101‑1 (MQ=255)
                                                                             ccGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTg                         <  2:3162869/101‑1 (MQ=255)
                                                                             acgaaccgTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTg                         <  1:2931733/94‑1 (MQ=255)
                                                                               gAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTc                       <  1:7159265/101‑1 (MQ=255)
                                                                               gAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTc                       >  2:7134687/1‑101 (MQ=255)
                                                                                 aaaGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAg                     <  2:5668255/101‑1 (MQ=255)
                                                                                 aaaGTAGCGCCCCCTCGCG.CCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAgg                    >  1:7691886/1‑101 (MQ=255)
                                                                                  aaGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAgg                    >  2:5339830/1‑101 (MQ=255)
                                                                                   aGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAggg                   >  1:600222/1‑101 (MQ=255)
                                                                                       gcgcCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTg               >  2:4983347/1‑101 (MQ=255)
                                                                                         gcCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTgag             >  2:1371371/1‑101 (MQ=255)
                                                                                           ccccTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGAt           <  1:6209144/101‑1 (MQ=255)
                                                                                              cTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCg        >  1:8186174/1‑101 (MQ=255)
                                                                                              cTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCg        <  1:2583264/101‑1 (MQ=255)
                                                                                               tCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGt       <  2:1256012/101‑1 (MQ=255)
                                                                                                cgcgCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGtt      >  2:9084499/1‑101 (MQ=255)
                                                                                                  cgcCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGtttt    <  1:341895/101‑1 (MQ=255)
                                                                                                  cgcCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGtttt    <  2:3986530/101‑1 (MQ=255)
                                                                                                  cgcCCCCCCCGCAAAACACAGGTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGtttt    <  2:4856717/101‑1 (MQ=255)
                                                                                                   gcCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGTTTTc   >  2:6215032/1‑101 (MQ=255)
                                                                                                   gcCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGTTTTc   <  1:1544367/101‑1 (MQ=255)
                                                                                                   gcCCCCCCCGCAAAACACAGTTTTCGCTTGCAGATTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGTTTTc   >  1:4766164/1‑101 (MQ=255)
                                                                                                    ccccccccGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGTTTTCg  >  2:1562878/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCAGTGGAAGGCGCTGACCGGGGCGGGCACCGCGGCGCAATACTGGCGCGAGGAAAGCGGGCGCTGGCAGAAGAAGGCCGAAAAGTAGCGCCCCCTCGCGCCCCCCCCGCAAAACACAGTTTTCGCTTGCAGCTTTGGCGCGGGGCCTTATGGTCCGGTCTCACCTCGGGGTGCTCTGTCAGGGGCTGAGATGCGTTTTCG  >  NC_014034/2889341‑2889541

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG