Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 290,303 Δ1 bp 1.9% intergenic (‑36/+14) RCAP_rcc00253 ← / ← RCAP_rcc00254 major facilitator superfamily protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034290,3030G→. 1.9% 11.9 ‑2.8 ‑0.7359intergenic (‑36/+14)RCAP_rcc00253/RCAP_rcc00254major facilitator superfamily protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (166/186):  total (172/187)
Fisher's exact test strand distribution p-value = 5.81e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.81e-01

Only 100 of 428 total aligned reads displayed.

AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403
                                                                                                    |                                                                                                    
aaGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCg                                                                                                      <  2:7011899/101‑1 (MQ=255)
 aGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgg                                                                                                     <  2:8008481/101‑1 (MQ=255)
  gCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggg                                                                                                    >  1:1985128/1‑101 (MQ=255)
      aGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggggg                                                                                                <  2:3837448/101‑1 (MQ=255)
       gCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggggg                                                                                               <  1:6598901/101‑1 (MQ=255)
       gCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggggg                                                                                               >  1:9079780/1‑101 (MQ=255)
           cAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTg                                                                                           >  1:3710755/1‑101 (MQ=255)
            aaGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGc                                                                                          >  1:1486797/1‑101 (MQ=255)
            aaGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGc                                                                                          <  1:247310/101‑1 (MQ=255)
            aaGATGAGGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGc                                                                                          <  2:4604974/101‑1 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      <  2:1302994/101‑1 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      <  1:4839879/101‑1 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      >  2:502512/1‑101 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      <  1:277693/101‑1 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      >  1:1191266/1‑101 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     >  1:8305839/1‑101 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     >  1:7812969/1‑101 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     >  2:4021145/1‑101 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     >  2:8873218/1‑101 (MQ=255)
                  atgGCGCCGCCCACAAGGACGGGGGTGATAAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAAt                                                                                    >  2:6237563/1‑101 (MQ=255)
                   tgNCGCCGCCCACAAGGNCGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATc                                                                                   <  2:1026026/101‑1 (MQ=255)
                       gccgccCACAAGGACGGGGTTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  2:2666566/101‑1 (MQ=255)
                       gccgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  1:1089888/101‑1 (MQ=255)
                       gccgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  2:7752982/101‑1 (MQ=255)
                       cccgccCGCAAGGACGGGGGTGATCGGGCGGGTCTGGGGCATTTGGCCTCCCTCGCAAGGCGGCCAGCTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  1:6462728/100‑1 (MQ=255)
                          gccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAt                                                                            <  1:7709525/101‑1 (MQ=255)
                           cccACAAGGGCGGGGGTTATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAtt                                                                           <  2:8458366/101‑1 (MQ=255)
                            ccACAAGGACGGGGGTGATCGGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtg                                                                          <  2:2842211/101‑1 (MQ=255)
                            ccACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtg                                                                          >  2:6850489/1‑101 (MQ=255)
                             cacaAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtgt                                                                         <  1:6882473/101‑1 (MQ=255)
                              acaAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtgtg                                                                        >  1:5908034/1‑101 (MQ=255)
                              acaAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtgtg                                                                        >  1:5457595/1‑101 (MQ=255)
                                aaGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCATCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCGGCGTCAATCTGTCGATTGCGcc                                                                      >  2:4425345/1‑101 (MQ=255)
                                  ggACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGcccc                                                                    <  1:7262652/101‑1 (MQ=255)
                                  ggACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCg                                                                   >  2:2902080/1‑101 (MQ=255)
                                    aCGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTTCCCCGa                                                                  >  2:1168984/1‑101 (MQ=255)
                                    aCGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGaa                                                                 >  1:3982611/1‑101 (MQ=255)
                                      gggggTTATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                <  1:4563459/101‑1 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                <  1:8122408/101‑1 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                >  1:1014483/1‑101 (MQ=255)
                                        gggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACaa                                                              <  1:1286227/101‑1 (MQ=255)
                                         ggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAAt                                                             <  1:6246890/101‑1 (MQ=255)
                                           tGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcg                                                           >  1:8757652/1‑101 (MQ=255)
                                           tGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcg                                                           >  2:1683109/1‑101 (MQ=255)
                                           ggATCATGCGGGTCTGGGTCATTTTGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcg                                                           <  1:4248799/100‑1 (MQ=255)
                                            gATCATGCGGGTCTGGGTCATTGGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcgc                                                          <  1:7090036/101‑1 (MQ=255)
                                            gATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcgc                                                          >  1:2275052/1‑101 (MQ=255)
                                            gATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcgc                                                          <  1:8873624/101‑1 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       >  1:3249776/1‑101 (MQ=255)
                                                 ggCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAGCAATCGcgccgc                                                     >  2:5760743/1‑101 (MQ=255)
                                                  gCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgcc                                                    <  2:7574367/101‑1 (MQ=255)
                                                  gCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgcc                                                    <  2:6467258/101‑1 (MQ=255)
                                                  gCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgcc                                                    <  2:3242803/101‑1 (MQ=255)
                                                   cGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgccg                                                   <  2:1628797/101‑1 (MQ=255)
                                                      gTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                <  1:6054842/101‑1 (MQ=255)
                                                      gTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                >  1:2179824/1‑101 (MQ=255)
                                                      gTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                >  1:1781911/1‑101 (MQ=255)
                                                      gTCTGGGCCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                <  2:1843088/101‑1 (MQ=255)
                                                       tCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTg                                               <  2:7812636/101‑1 (MQ=255)
                                                       tCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTg                                               <  1:8622073/101‑1 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGTCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             <  2:3934452/101‑1 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             >  2:222278/1‑101 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             >  2:8183104/1‑101 (MQ=255)
                                                          gggTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGccc                                            >  1:2970473/1‑101 (MQ=255)
                                                          gggTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGccc                                            >  1:3353911/1‑101 (MQ=255)
                                                           ggTCATTTGGTCCCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCg                                           <  2:865526/101‑1 (MQ=255)
                                                            gTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCgg                                          <  1:4108721/101‑1 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        <  2:3387728/101‑1 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        >  1:1928090/1‑101 (MQ=255)
                                                                tttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAc                                      >  1:834884/1‑101 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcg                                     <  2:1932233/101‑1 (MQ=255)
                                                                  tGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgc                                    >  1:6703252/1‑101 (MQ=255)
                                                                  tGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgc                                    <  2:4827969/101‑1 (MQ=255)
                                                                  tGGTCCCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgc                                    <  1:5302412/101‑1 (MQ=255)
                                                                      ctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGcc                                >  1:2535586/1‑101 (MQ=255)
                                                                      ctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGcc                                <  2:5664727/101‑1 (MQ=255)
                                                                      ctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGCcc                                >  1:4018406/1‑101 (MQ=255)
                                                                       tcCCTCTCAAGGCGGCCAGTTCGCCATGCGGGCGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGccc                               <  2:6762421/101‑1 (MQ=255)
                                                                          cTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTc                            >  1:5649356/1‑101 (MQ=255)
                                                                            cGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCNGNCgn                          >  1:2095940/1‑100 (MQ=255)
                                                                            cGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGc                          >  2:7411954/1‑101 (MQ=255)
                                                                             gCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGcc                         >  1:5090596/1‑101 (MQ=255)
                                                                               aaGGCGGCCCGTTGGCCATGCGGGGGCGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAg                       <  2:5353654/101‑1 (MQ=255)
                                                                               aaGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAg                       <  2:2533478/101‑1 (MQ=255)
                                                                                aGGCGGCCAGTTCGCCATGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGa                      <  2:3710646/101‑1 (MQ=255)
                                                                                 ggcggcCATTTGGCCGGGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGAc                     <  2:9079358/101‑1 (MQ=255)
                                                                                  gcggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACa                    <  2:2082046/101‑1 (MQ=255)
                                                                                   cggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAg                   <  2:8849882/101‑1 (MQ=255)
                                                                                    ggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGc                  <  1:5252717/101‑1 (MQ=255)
                                                                                     gcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGcc                 <  2:4192972/101‑1 (MQ=255)
                                                                                       cAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCgg               <  2:6584790/101‑1 (MQ=255)
                                                                                        aGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGt              >  1:7459101/1‑101 (MQ=255)
                                                                                         gTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTc             <  2:5907799/101‑1 (MQ=255)
                                                                                         gTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTc             <  2:356610/101‑1 (MQ=255)
                                                                                         gTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTc             <  1:7563192/101‑1 (MQ=255)
                                                                                            cGCCAGGCGGGGGGGGGCTGGGTCCATGGGGCGATGGTGCCCGGAACACCGACGCCGCCGCCTGCCCGGCGACGCGGCGCGTCGCCAGACAGCCGGTcagc          <  2:3775945/101‑1 (MQ=255)
                                                                                             gCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagca         <  2:2274985/101‑1 (MQ=255)
                                                                                                aTGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGAt      <  1:8486812/101‑1 (MQ=255)
                                                                                                 tGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATa     <  2:5452573/101‑1 (MQ=255)
                                                                                                   cGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAgc   >  1:4646445/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG