Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,024,766 Δ1 bp 100% intergenic (‑176/‑152) chvI ← / → pckA transcriptional regulator ChvI/phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,024,7660T→.100.0% 2376.0 536intergenic (‑176/‑152)chvI/pckAtranscriptional regulator ChvI/phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  new base (256/280):  ref base (0/0):  total (256/280)

Only 100 of 579 total aligned reads displayed.

TGCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCCC  >  NC_014034/3024666‑3024862
                                                                                                    |                                                                                                
 gCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtt                                                                                                 <  1:8565128/101‑1 (MQ=255)
  cGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGttt                                                                                                <  2:1882970/101‑1 (MQ=255)
   gAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttg                                                                                               <  2:4580267/101‑2 (MQ=255)
    aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                              <  1:6811581/101‑3 (MQ=255)
    aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                              >  2:3962309/1‑99 (MQ=255)
    aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                              >  2:7226730/1‑99 (MQ=255)
       cGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCt                                                                                          <  2:6533226/101‑1 (MQ=255)
       cGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCt                                                                                          <  2:3517284/101‑1 (MQ=255)
        gCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTa                                                                                         <  2:5973807/101‑1 (MQ=255)
        gCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTa                                                                                         <  1:8236515/101‑1 (MQ=255)
           cACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCt                                                                                      >  2:4753807/1‑101 (MQ=255)
           cACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCt                                                                                      <  1:1137685/101‑1 (MQ=255)
           cACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCt                                                                                      >  1:3273691/1‑101 (MQ=255)
           cACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCt                                                                                      <  1:7105475/101‑1 (MQ=255)
            aCGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTg                                                                                     <  1:1208537/101‑1 (MQ=255)
            aCGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTg                                                                                     >  1:4916992/1‑101 (MQ=255)
             cGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGc                                                                                    >  2:2501720/1‑101 (MQ=255)
              gTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCg                                                                                   >  2:5349050/1‑101 (MQ=255)
              gTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCg                                                                                   <  1:4388064/101‑1 (MQ=255)
                gATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGAc                                                                                 >  2:3667211/1‑101 (MQ=255)
                gATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGAc                                                                                 <  1:8912685/101‑1 (MQ=255)
                      aaTCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAccc                                                                           >  1:5822679/1‑101 (MQ=255)
                      aaTCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAccc                                                                           <  1:5643895/101‑1 (MQ=255)
                      aaTCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAccc                                                                           <  2:7095982/101‑1 (MQ=255)
                      aaTCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAccc                                                                           >  2:2708984/1‑101 (MQ=255)
                         catcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTc                                                                        <  1:1877117/101‑1 (MQ=255)
                           tcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTg                                                                      >  2:1035586/1‑101 (MQ=255)
                            ttggtccgaaaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGt                                                                     <  2:2969043/93‑1 (MQ=255)
                             tGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGtt                                                                    <  2:3794337/101‑1 (MQ=255)
                             tGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGtt                                                                    <  1:4086242/101‑1 (MQ=255)
                             tGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGtt                                                                    >  1:1340853/1‑101 (MQ=255)
                             tGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGtt                                                                    >  2:5123867/1‑101 (MQ=255)
                               aGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTcc                                                                  <  2:1774679/101‑1 (MQ=255)
                                 cgcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCtt                                                                <  1:6166183/101‑1 (MQ=255)
                                  gcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTc                                                               <  1:7227034/101‑1 (MQ=255)
                                   cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                              <  2:170054/101‑1 (MQ=255)
                                   cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                              <  1:6306422/101‑1 (MQ=255)
                                   cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                              <  2:8106157/101‑1 (MQ=255)
                                     aaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCa                                                            <  1:5123683/101‑1 (MQ=255)
                                       aaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGTTACGTGCGACAAACCCTTCTGGTCCTTCGCact                                                          >  1:502/1‑99 (MQ=255)
                                       aaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAt                                                          >  1:8443522/1‑101 (MQ=255)
                                       aaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAt                                                          >  1:3518115/1‑101 (MQ=255)
                                        aaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAtt                                                         >  2:8174062/1‑101 (MQ=255)
                                        aaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAtt                                                         >  1:1659696/1‑101 (MQ=255)
                                        aaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAtt                                                         <  1:3416141/101‑1 (MQ=255)
                                        aaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAtt                                                         <  1:1522401/101‑1 (MQ=255)
                                        aaaCGAAGGTTTGACCAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGCTCCTTCGCAAtt                                                         <  2:6692604/101‑1 (MQ=255)
                                         aaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTg                                                        <  2:3456311/101‑1 (MQ=255)
                                         aaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTg                                                        <  2:8797101/101‑1 (MQ=255)
                                          aCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgc                                                       <  1:2267427/101‑1 (MQ=255)
                                            gAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgcgc                                                     >  1:2395749/1‑101 (MQ=255)
                                            gAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgcgc                                                     >  2:4128799/1‑101 (MQ=255)
                                              aGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCa                                                   <  1:6641800/101‑1 (MQ=255)
                                               ggTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCAt                                                  <  1:2920592/101‑1 (MQ=255)
                                               ggTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCAt                                                  <  1:4492925/101‑1 (MQ=255)
                                                gTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATg                                                 >  2:489953/1‑101 (MQ=255)
                                                 tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCAtag                                                >  1:3927067/1‑99 (MQ=255)
                                                 tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATgg                                                >  2:560335/1‑101 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             <  1:1004865/101‑1 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             <  1:3114228/101‑1 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             <  1:8054693/101‑1 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             <  1:7885178/101‑1 (MQ=255)
                                                      cTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAATTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCAGTTCCTTCGCAATTGTGCCATGGTATAg                                           <  1:6515735/101‑1 (MQ=255)
                                                      cTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAg                                           <  2:898164/101‑1 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          >  1:199614/1‑101 (MQ=255)
                                                        aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                         >  2:5080225/1‑101 (MQ=255)
                                                        aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                         >  2:4416221/1‑101 (MQ=255)
                                                         aTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgc                                        >  1:1753269/1‑101 (MQ=255)
                                                         aTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgc                                        <  1:9198532/101‑1 (MQ=255)
                                                          tttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcg                                       >  2:8390258/1‑101 (MQ=255)
                                                          tttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcg                                       >  1:4163109/1‑101 (MQ=255)
                                                            tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGcc                                     >  1:5362094/1‑101 (MQ=255)
                                                               ctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATc                                  <  2:5816316/101‑1 (MQ=255)
                                                               ctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATc                                  >  1:2188457/1‑101 (MQ=255)
                                                                tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATcc                                 <  2:7919405/101‑1 (MQ=255)
                                                                tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATcc                                 >  2:3341803/1‑101 (MQ=255)
                                                                tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATcc                                 <  2:2120058/101‑1 (MQ=255)
                                                                 cACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCg                                >  2:2060106/1‑101 (MQ=255)
                                                                     tctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAAc                            <  1:3793670/101‑1 (MQ=255)
                                                                      ctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                           >  2:8597252/1‑101 (MQ=255)
                                                                      ctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                           >  2:7814355/1‑101 (MQ=255)
                                                                       ttCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAt                          <  2:1428993/101‑1 (MQ=255)
                                                                        tCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAtt                         <  2:631807/101‑1 (MQ=255)
                                                                           cgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCtt                      <  2:2617744/101‑1 (MQ=255)
                                                                            gatgatGGGGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAATCCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTg                     <  2:1303285/101‑1 (MQ=255)
                                                                            gatgatGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTg                     >  2:689436/1‑101 (MQ=255)
                                                                             atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCTCCATGGTATAGTGCGCCATCCGCAACATTCTTgc                    >  2:1188844/1‑101 (MQ=255)
                                                                             atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgc                    <  2:2267529/101‑1 (MQ=255)
                                                                             atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgc                    <  1:8712040/101‑1 (MQ=255)
                                                                             atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgc                    >  1:4737981/1‑101 (MQ=255)
                                                                               gatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcgc                  >  2:7602958/1‑101 (MQ=255)
                                                                                atgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGcgcg                 >  1:2849701/1‑101 (MQ=255)
                                                                                atgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGAATAGGCCGCCATCCGCAACCTTCTTgcggg                 >  2:1538169/1‑99 (MQ=255)
                                                                                 tgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCgg                <  2:9070162/101‑1 (MQ=255)
                                                                                    cgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATc             <  1:2649279/101‑1 (MQ=255)
                                                                                       tAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGt          >  2:4341847/1‑101 (MQ=255)
                                                                                          aTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcc       <  2:8691447/101‑1 (MQ=255)
                                                                                            cGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccg     >  2:7448184/1‑101 (MQ=255)
                                                                                            cGGCCCT.TTTTGCGCTAGCTCCGACAAACCCGTCTTTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccg     <  1:7347942/101‑1 (MQ=255)
                                                                                             ggCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgc    >  1:7573416/1‑101 (MQ=255)
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TGCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCCC  >  NC_014034/3024666‑3024862

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG